巴西橡胶树若干品系叶切片的超微结构观察

本文对巴西橡胶的若干品系叶片进行了显微和超微结构的观察。实验结果表明：巴西橡胶不同品系间的叶片解剖结构存在明显差异：古农９６－２８，ＲＲＩＭ６００和ＩＡＮ８７３叶片维管束鞘细胞含有丰富的叶绿体，基粒片层较发达，且韧皮部薄壁细胞，木质部薄壁细胞和射线细胞也含有叶绿体，但没有典型的“花环型”结构；ＩＡＮ８７３叶片维管束鞘细胞里富含两种类型的光合膜、线粒体和发达的内质网等多种细胞器。而天任 ３１－４５等的叶片鞘细胞仅含少量叶绿体，其片层不发达，且无基粒

Construction of Donor Plasmid in the Gene Integration Platform System for Oryza sativa L.

提高水稻产量，改良稻米品质是育种学家广泛研究的课题.随着现代生物技术的发展，水稻已成为植物基因工程的重要研究对象.许多实验室已成功地建立了一系列供外源基因转化水稻的系统.但是这些转化系统主要应用TI质粒衍生的载体，通过T-dnA左右两端的序列将目的基...Many laboratories have set up several foreign gene transformation systems for rice.But these transformation systems were all used T DNA system of Ti plasmid, then the foreign gene integrated into plant genomes DNA by means of the insertion of both ends of T DNA.The integrated target of T DNA in plant genome DNA is random, while the thoroughly random integration would interfere with self stable system of plant genome, which could lead to pernicious mutation.In this study, according to the mechanism of homologous recombination, we had constructed a new donor plasmid for indica rice.It would cause the foreign gene intergated into chromsomal rDNA locus. According to the known sequence of ribosomal DNA, we cloned the 2.5kb fragment of it by PCR, and used the fragment as the integrated homologous sequence.After subcloning foreign DNA including nptII gene and metallothionein gene at the end of homologous fragment, the donor plasmid pURKMT which will integrate into the genomes DNA of indica rice was constructed.Then, donor plasmid pURKMT was introduced into calli of indica rice (Jiahe NO.7) by electroporation.Screened by kanamycin, the transformant calli which was integrated the foreign DNA were selected.Dot blotting data showed that the foreign DNA including nptII gene and MT gene has integrated into rice genome DNA

Application of immunofluorescence to C_3/C_4 attribute identification of a tropical crop, Coffea arabica

用从烟草提纯的 1,5-二磷酸核酮糖 ( Ru BP)羧化酶制备兔抗 Ru BP羧化酶抗体 ,并以异硫氰酸盐荧光素 ( FITC)标记抗体 .采用直接免疫荧光法对典型 C3植物水稻、C4 植物甘蔗和小粒种咖啡等进行了 Ru BP羧化酶的组织化学定位 .结果表明 :C3和 C4 植物叶切片中 Ru BP羧化酶的分布明显不同 ,C3植物的特异荧光位于叶肉细胞 ,C4 植物的特异荧光绝大部分位于维管束鞘细胞 ;小粒种咖啡的特异荧光仅分布在叶肉细胞 .因此认为 ,小粒种咖啡应属 C3植物Rabbit antiserum raised purified ribulose 1,5 bisphosphate carboxylase (RuBPCase) from tobacco was used to locate RuBPCase in leaf blade transection of classical C 3, C 4 plants and Coffea arabica by direct immunofluorescence method. The antibody was labelled by fluoresecin isothiocyanate (FITC). It was discovered that in classical C 4 plant (sugarcane), the specific fluorescence was located almost exclusively in bundle sheath cell chloroplasts, while in C 3 plant (rice), the specific fluorescence was in mesophyllous cell chloroplasts, which proved the difference in RuBPCase location between classical C 4 and C 3 plants. The specific fluorescence of C.arabica was located only in mesophyllous cell chloroplasts, so it was concluded that C.arabica belongs to C 3 plant

Rapid Propagation of Anoectochilus roxburghii

以金线莲(Anoectochilus roxburghii)试管苗带节茎段和顶芽为外植体,用正交试验筛选芽快速增殖的培养基配方,并研究生根粉(ABT)对促根壮苗的作用。结果表明,丛生芽增殖最佳配方为MS+BA 3.5mg/L+KT 1.5mg/L+NAA 0.6mg/L,两个月增殖倍数达3.5;3mg/L 3号生根粉对金线莲生根壮苗效果最佳,使植株生根条数、最长根、植株增高和增叶数分别比对照增加12.8%、34.1%、13.2%和22.4%,从而提高移栽成活率,3种不同基质均达到94%以上。Taking stem segments and apical buds as explants, the optimal culture medium for rapid propagation of Anoectochilus roxburghii were carried out by orthogonal screening experiment. MS + BA 3.5mg/L + KT 1.5mg/L + NAA 0.6mg/L gave the best result for multiplication of adventitious buds and proliferated buds for 3.5 times within 2 months. The growth media supplemented with ABT3 3mg/L increased the number of roots, the length of roots, the height of seedlings and the number of leaves by 12.8%, 34.1%, 13.2% and 22.4%, respectively.福建省漳州市科委项目(Z02028)资

福建省实验动物学学科发展研究报告

1实验动物科学概述1.1实验动物科学的概念实验动物科学(lAbOrATOry AnIMAl SCIEnCES)是以实验动物资源研究、质量控制和利用实验动物进行科学实验的一门综合性学科。在《学科分类与代码标准》(gb/T13745-1992)中,与实验动物科学有关的学科分别是“实验动物学“、“医学实验动物学“、“比较病理学“等

吉西他滨联合化疗一线治疗晚期胰腺癌生存结果的亚组meta分析

【目的】Meta分析提示吉西他滨（GEM）联合化疗一线治疗晚期胰腺癌优于标准的GEM单药化疗,在此基础上进行亚组生存结果的meta分析及资料更新,旨在寻找确切有效的化疗方案。【方法】通过MEDLINE、EMBASE、ASCO、ECCO等数据库及论文集检索相关文献。按纳入标准筛选新增文献并进行资料更新。主要对各亚组进行半年生存率、其次是1年生存率的meta分析。【结果】17个随机对照临床试验（RCT）共3 821例患者纳入分析,按化疗方案分为GEM联合顺铂（GEMDDP）、GEM固定剂量率输注联合奥沙利铂（GEMOX）、联合5FU（GEMFU）、联合卡培他滨（GEMCAP）以及联合伊立替康（GEMIRI）等5个亚组,各亚组半年生存率的治疗优势（RD）分别为5%（P=0.24）、9%（P=0.005）、2%（P=0.46）、7%（P=0.03）和-1%（P=0.88）;1年生存率RD为6%（P=0.11）、5%（P=0.07）、4%（P=0.19）、5%（P=0.08）和0（P=0.97）。【结论】现有的证据提示GEMOX、GEMCAP联合化疗方案一线治疗晚期胰腺癌,有较好的应用前景,值得进一步的临床试验

Analysis on the epidemiological characteristics of the sporadic cases with hepatitis E infection in the rural area in Jiangsu province

目的了解江苏省农村散发性戊型肝炎的流行病学特征。方法通过建立覆盖市、镇、村三级医疗卫生服务机构的疑似肝炎主动监测网络,系统全面地监测戊型肝炎病例的发病情况。结果主动监测网络的敏感性明显高于网络报告系统,能更加准确、全面地掌握戊肝的发病规律。结果显示戊型肝炎病例占疑似急性肝炎病例的26.7%,男性戊肝发病率高于女性(P<0.01);发病随着年龄增长而上升,多见35岁以上人群;全年均有发病,冬春季节较高;戊型肝炎病毒株HEV1、4型并存,但以HEV4型为主(92.5%)。结论疑似肝炎主动监测系统数据显示目前戊肝发病率有被低估的风险。To understand the epidemiological characteristics of the sporadic cases with hepatitis E infection in the rural area of Jiangsu province,the prevalence of hepatitis E infection was surveyed systemically and completely through the initiatively monitoring system for the suspected cases of hepatitis E covering the city,town and village levels of medical and health service organizations.It was found that the initiatively monitoring system for suspected hepatitis E cases reflected the incidence of hepatitis E more accurately and comprehensively and was proved to be more sensitive than the network reporting system.The cases with hepatitis E accounted to 26.7% of the suspected cases with acute hepatitis and they were more frequent in male than in female.The incidence of hepatitis E increased with advance of age and most of cases occurred with age over 35.Estimated incidence was stable on the whole year,but rose up in winter and spring.The HEV type 1 and type 4 co-existed,but the HEV type 4 was proved to be the main type(92.5%).From the above observations,it seems that the initiatively monitoring system for the suspected cases of hepatitis E may be under-estimated for the risk of the hepatitis E infection in rural area of Jiangsu province.国家高技术研究发展计划(863计划)(2006AA02A209)资

散发型戊型肝炎与急性乙肝临床特征比较

目的比较散发型急性戊型肝炎与乙肝临床特征。方法分析戊肝和急性乙肝间年龄、性别、肝脏损伤、症状体征以及病程的差异。结果急性肝炎病例中,戊型肝炎最常见(28.0%),急性乙肝次之(9.2%);戊肝平均发病年龄为56.3±13.1岁,急性乙肝为43.0±12.5岁,平均发病年龄戊肝较急性乙肝大(t=4.4723,P<0.0001),均为男性多发;两者常见的临床症状和体征基本相当,但戊肝患者黄疸症状更多见(P<0.05);戊肝病程较急性乙肝长(t=3.7249,P=0.0003);通过年龄性别进行1:1匹配分析,戊肝比急性乙肝对肝脏的损伤程度严重(t=3.5978,P=0.0019)。结论戊肝多见于中老年,急性乙肝多见于中青年,均为男性多于女性。临床特征比较戊肝较急性乙肝更为严重

PHYSIOLOGICAL RESPONSES of HEVEA BRASILIENSIS DURING THE CHILLING INJURY

在人工零上低温下,巴西橡胶树(HEVEAbrASIlIEnSIS)叶质膜透性随低温处理时间的延长而持续上升,抗冷品系的上升速率比不抗冷品系慢。呼吸强度和ATP含量均随处理时间的延长而持续下降,抗冷品系下降速率比不抗冷品系慢。叶绿体Mg-(++)—ATPASE活性也表现出明显抗性差异。可见,低温下叶组织的质膜透性、呼吸强度、ATP含量以及Mg-(++)—ATPASE活性的变化与品系抗冷性有关。低温下呼吸强度、ATP含量与质膜透性变化呈负相关,质膜透性的变化与供能有关。Under the reFrigerating conditions ( low temperatures of above oC ) , the permeability of plasma membrane in Hevea brasiliensis was rising steadily with the prolongation of low temperature treatment, and the rising rates of chilling resistant clones were slower than those of chilling-sensitive clones, The respiratory intensity and ATP content were dropping with the prolongation of treatment time, and the dropping rates of chilling resistant clones were slower than those of chilling sensitive clones.The resistant clones alones also displayed a noticeable change in the activities of chloroplasts Mg ++-ATPase, obviously diFFerent From those of the sensitive clones.It is thus seen that the changes of the permeability of plasma membrane, respiratory intensity, ATP content and Mg++-ATPase activity are related to the chilling resistance of clones.The respiratory intensity and ATP content under low temperature negatively related to .the change of plasma membrane permeability which has a bearing on the supply of energy