Study on Bioactivity of Endophytic Fungi from Mangrove Plants and Secondary Metabolites Produced by Fungus A-1-2-3

近年来，随着研究热点的转移，新药的研究开发已突破了陆生资源的束缚，拓展到了生态和物种远比陆地生境复杂多样的海洋。红树植物生长于海岸潮间带，其独特的生存环境赋予它丰富的微生物资源，是当前倍受重视的药用新资源。本文对福建省九龙江口浮宫镇草埔头红树林区木榄(Bruguieragymnorrhiza)和秋茄(Kandeliacandel)2种红树植物的内生真菌资源进行为期1年的种群动态调查并对这些菌株的抗菌、抗肿瘤、乙酰胆碱酯酶及胰蛋白酶抑制剂活性进行了初步研究。对活性菌株A-1-2-3的代谢产物进行分离纯化并对分离得到的化合物进行结构鉴定和生理活性研究。为深入开发和保护红树植物内生真菌药用资源提供理...The hot spot for searching novel drugs has been focused on the much more complex marine resources instead of the traditional terrene habitat in recent years. Mangrove plants as important new resources for potential pharmaceutical because of their ecosystems that straddled the land and the sea, from freshwater to seawater, often with distinct microorganism species, have gradually attracted more and...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_微生物学学号：20032604

西藏不同居群大花黄牡丹的种子油脂成分分析

【目的】对不同居群大花黄牡丹油脂成分进行评价,揭示该种是否适合作为油用牡丹开发的新材料。【方法】使用气质联用色谱法(GC-MS)对采自西藏林芝和山南地区5个居群的混合种子以及2个居群的单株进行了油脂成分分析。【结果】大花黄牡丹籽油富含不饱和脂肪酸(86.32%),油酸>α-亚麻酸>亚油酸;林芝地区4个居群油脂成分接近,各脂肪酸成分显著高于山南居群;居群内单株油脂成分林芝1号居群内部无差异,山南居群内部差异明显。【结论】野生大花黄牡丹油脂成分和组成与其他栽培种油用牡丹差异较大,油酸含量较高,亚麻酸含量较低,更有利于保存,大花黄牡丹可以作为油用牡丹开发的新材料

The Population Fluctuation and Bioactivity of Endophytic Fungi from Mangrove Plants in Different Seasons

2003年10月至2004年8月对福建龙海浮宫2种红树植物秋茄、木榄的内生真菌进行连续6次的分离,得到290株内生真菌.研究结果显示,木榄(Bruguiera gymnorrhiza)和秋茄(Kandelia candel)内生真菌数量高峰期分别为4月份和10~12月份;青霉(Penicillium)、交链孢(Alternaria)、Dothiorella和无孢目是秋茄中的主要优势属;木榄内生真菌主要优势属为头孢霉(Cephalosporium)、交链孢(Alternaria)、青霉(Penicillium)等.不同宿主对内生真菌的季节分布有重要的影响.抗菌测定共筛选得到活性菌株40株,占总菌株数的13.8%;17株内生真菌对KB(人口腔上皮癌)和/或Raji(人B淋巴瘤)细胞具有抑制作用,占总菌株数的5.9%.生物活性菌株分布于青霉、交链孢、拟青霉、无孢目等10个不同的分类单元中,2种宿主植物内生真菌抗菌及抗肿瘤活性均有不同程度的季节差异性.From December 2003 to August 2004,the endophytic fungi of Kandelia cande and Bruguiera gymnorrhiza,which were collected from Fugong in Fujian Province,were isolated for six times.290 strains endophytic fungi were obtained.The results showed the number fastigium of endophytic fungi from Kandelia candel and Bruguiera gymnorrhiza were in April and October to December respectively.Penicillium?Alternaria?Dothiorell and the nonsporulating groups were the dominant genuses of the endophytic fungi from Kandelia cande,while the endophytic fungi from Bruguiera gymnorrhiza mainly belonged to Cephalosporium?Alternaria?Penicillium,and so on.The differences of the host played an important role in the attribution of the temporal variations of different endophytic fungi.In the investigation of antimicrobial activities,40 strains(13.8% of the total isolates) with antimicrobial activities were screened out.17 strains(5.9% of the total isolates) could inhibit the growth of Raji and/or KB tumor cell lines.The active strains obtained mostly belonged to Penicillium,Alternaria,Paecilomyces and other 7 genuses.The endophytic fungi from 2 mangrove plants also displayed seasonal variations in their antimicrobial and antitumor activities.国家863计划(2003AA624010);; 福建省科技计划项目(2004N003)资

Fine root morphology of different branch orders of three dominant subalpine tree species in western Sichuan

林木细根(直