铜绿微囊藻高温胁迫后的超补偿生长

将在40℃条件下培养5d和10d的铜绿微囊藻(Microcystis aeruginosa FACHB905)转接至新鲜培养基,以25℃培养条件下转接的相同细胞密度的培养物为对照,然后均置于相同的培养条件下恢复生长.研究了各培养物的生长、叶绿素a浓度、光系统Ⅱ的光化学效率(Fv/Fm)、净光合速率和呼吸速率的变化.结果表明,在40℃高温下分别培养5d和10d,铜绿微囊藻的比生长速率、Fv/Fm等指标均受到显著抑制(p<0.01).但在胁迫解除后的中后期,40℃培养5d组的比生长速率为0.362,显著高于

高效液相色谱法测定母乳中唾液酸含量

建立荧光高效液相色谱(fluorescence detector-high performance liquid chromatography,HPLC-FLD)测定母乳中唾液酸N-乙酰神经氨酸(N-acetylneuraminic acid,Neu5Ac)和N-羟乙酰神经氨酸(N-glycolyl neuraminic acid,Neu5Gc)含量的分析方法。利用酸水解法释放出母乳中的唾液酸,以4,5-亚甲二氧基-1,2-邻苯二胺盐(4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride,DMB)为衍生化试剂,50℃避光衍生150min,采用荧光高效液相色谱仪检测。色谱条件:LiChrosorb RP-18柱(250mm×4mm,5μm),流动相为甲醇-乙腈-超纯水(7:8:85),流速0.9mL/min,进样体积10μL,柱温30℃,荧光检测器激发波长373nm,发射波长448nm。结果表明:唾液酸在50～400μmol/L范围内与唾液酸峰面积的线性关系良好,平均回收率为94.0%,精密度的相对标准偏差(relative standard deviation,RSD)为0.4%,稳定性RSD为1.0%,重复性RSD为0.8%,Neu5Ac的最低检出限为0.02μmol/L,Neu5Gc的最低检出限位0.03μmol/L。该方法简单、重复性好、灵敏度高,可广泛用于奶粉、牛奶及母乳中唾液酸含量测定

Culture medium optimization for algicidal strain Y35 and preparation of algicidal bacterial agents

塔玛亚历山大藻是一种有毒甲藻,常引发赤潮,严重威胁海洋生态的稳定和人类的健康,细菌Deinococcus sp.Y35表现出对塔玛亚历山大藻的杀藻能力,为促进菌株生长、提高杀藻效果并方便保存,对菌株Y35培养条件进行优化,并制备杀藻菌剂.分别确定菌株Y35生长所需的最适氮源、碳源、无机盐,并确定其最适添加量,在优化的基础上完成冻干菌剂的制备和最适冻干保护剂的选择.菌株Y35生长的最适培养基成分是1.0%胰蛋白胨和0.5%酵母粉.在优化的培养基基础上对菌株Y35进行发酵,培养至对数期后进行冷冻干燥,制备杀藻菌剂.菌株Y35需要添加1.0 g/L的蔗糖作为冻干保护剂.杀藻菌剂的杀藻添加量为2.0 mg/m L.本研究可为下一步将细菌应用于赤潮治理奠定基础.Alexandrium tamarense is a toxic dinoflagellate,which causes harmful algal blooms(HABs),posing a serious threat on marine ecology and human health.Deinococcus sp.Y35 shows algicidal activity on A.tamarense.This research aimed to optimize its culture medium and improve the preparation of bacterial agents.We determined the optimum nitrogen source,carbon source and inorganic salts of strain Y35,then decided the optimum adding amount,prepared the freeze drying bacterial agents,and chose the optimum freeze-drying protective agent.The optimum culture medium composition of strain Y35 was found to be 1% tryptone and 0.5% yeast extracts.We needed to add 1.0 g/L sucrose as a protective agent for bacterial culture of strain Y35 before freeze-drying.The optimum amount of algicidal agent was 2.0 mg/m L.The optimization of culture conditions could ensure the bacterial growth and algicidal activity.The results of this research provide some foundation for HABs control in the future.国家自然科学基金项目(41576109,31500095);; 河南师范大学博士科研启动基金(5101049170160)资助~

Function of a methyl-accepting chemotaxis protein Tlp1 in Azorhizobium caulinodans ORS571

【目的】考察茎瘤固氮根瘤菌中趋化基因簇上游的受体蛋白Tlp1编码基因的突变表型,初步探究其功能机理。【方法】利用同源重组和三亲本接合转移的方法构建突变株,在TY培养基中测定生长情况,半固体平板法观察趋化圈,刚果红固体培养基观察胞外多糖和次生代谢产物的分泌,乙炔还原法测定菌株的固氮酶活性。【结果】与野生型菌株相比,tlp1突变株的生长速率没有影响。在以甘油为碳源的L3半固体平板上突变株的趋化圈变小,其回补菌株能部分回补趋化能力。突变株的胞外多糖分泌与野生型没有区别,但其次生代谢产物黑色素出现的时间比野生型稍早。在固氮酶活性测定中,发现突变株酶活性明显比野生型降低,回补菌株能够部分回补。【结论】茎..

厦门地区乳母产褥期膳食营养状况调查

目的调查评价厦门地区乳母产褥期饮食结构和膳食营养状况,并与正常妇女比较,分析营养素摄入满足机体需要的程度以及存在的营养问题。方法采用24h膳食回顾法调查厦门地区40名乳母产后第2天、第7天和第30天的饮食状况,同样方法调查58名同龄健康非孕非产妇女的1日膳食做对照。参考中国食物成分表计算营养素的摄入,与相应人群的膳食营养参考摄入量(DRIs)比较进行评价。结果乳母产后第2天、第7天和第30天能量平均摄入分别达到推荐摄入量(RNI)的86.2%、75.8%和75.4%,对照组妇女能量摄入仅达到RNI的61.9%。乳母蛋白质平均摄入量分别高于RNI(85g)的55.8%、31.5%和22.6%,碳水化物摄入较少。乳母铁摄入量达到RNI标准,烟酸、维生素E和硒的摄入高于RNI水平,维生素C和钙的摄入低于RNI水平。乳母维生素A、维生素E和硒的摄入达RNI的比例高于对照组妇女比例,但维生素C、钙和锌的摄入比例较对照组低。结论乳母能量、蛋白质和脂肪的摄入基本符合DRIs的标准,但三大营养素供能比例不平衡,部分微量营养素摄入偏低,乳母3个时间点大部分营养素摄入变化趋势不明显,乳母大部分的营养素摄入水平较对照组妇女好

柴郁地仙方治疗围绝经期抑郁症体会

围绝经期抑郁症的发病基础是肾虚精血亏少,七情过极是诱因,病程中多表现为肝郁气滞、痰瘀交阻、闭塞脑窍。肾虚肝郁又常累及心脾二脏,故以补肾疏肝、化痰祛瘀、补益心脾立法,自拟柴郁地仙方治疗本病取得良好效果。从实验及临床研究结果来看,补肾疏肝、化痰祛瘀、补益心脾这种多脏腑整体调治,虚实兼顾治疗围绝经期抑郁症的思路较有效,且值得进一步推广和深入探讨。福建省卫生厅资助项目(WZPW101307);; 厦门市科技局资助项目(3502Z20133007

基于两步退火法提升Al/n~+Ge欧姆接触及Ge n~+/p结二极管性能

锗（Ge）中高激活浓度、低扩散深度的n型掺杂是实现高性能Ge n-MOSFET的重要前提条件.本文采用低温预退火与脉冲激光退火相结合的两步退火法,结合磷离子注入,制备Al/n+Ge的欧姆接触以及Ge n+/p结二极管.通过电流-电压特性测试来研究Al/n+Ge的欧姆接触以及Ge n+/p结二极管的性能,测试结果表明:低温预退火可初步修复注入损伤,并降低激光退火时杂质的扩散深度;结合离子注入工艺和两步退火工艺,Al/n+Ge欧姆接触的比接触电阻率降至2.61×10-6Ω·cm2,Ge n+/p结二极管在±1V的整流比提高到8.35×106,欧姆接触及二极管性能均得到了显著提升.福建省自然科学基金(批准号:2018J05115);;\n厦门理工学院高层次人才项目(批准号:YKJ16012R);厦门理工学院科研攀登计划项目(批准号:XPDKQ18027);;\n国家自然科学基金青年科学基金(批准号:61704142)资助的课题~

Diversity of culturable endophytic fungi of common reed ( Phragmites australis) in coastal wetland

内生真菌在植物生长以及抵御环境胁迫过程中起着非常重要的生态作用.本研究从黄河三角洲滨海湿地1350个芦苇组织切片中分离得到318株内生真菌,通过对rDNA ITS的分型、测序及系统进化分析,研究了该地区芦苇植株根、茎、叶中可培养内生真菌的种类组成及在不同盐度、不同组织中的分布情况.根据序列相似性(以98%为阈值),共获得12个真菌分类操作单元(OTUs).在门级分类水平上,子囊菌门为绝对优势菌群,在各组织和站位中均有分布.芦苇根组织中分离得到的内生真菌OTU数相对较多,叶组织和茎组织中分离到的OTU数相同,且叶中的OTU在根中均存在.潮上区(低盐区)内生真菌OTU数最多,高潮区次之.根中可培养..

Phytoplankton biomarkers in surface seawater from the northern South China Sea in summer 2009 and their potential as indicators of biomass/community structure

生物标志物已被广泛应用于重建浮游植物生产力和群落结构变化。该方法假设之一是生标的含量可以反映表层海水的浮游植物生物量,但这个假设还缺乏现场观测的充分验证。对2009年冬季南海北部表层海水颗粒物中主要生标做了分析,利用其含量及比值研究浮游植物的生物量及群落结构的分布。生标含量表明硅藻、甲藻、颗石藻的高值区位于珠江口南部及广东沿岸,在陆坡区也有高值。生标比值显示硅藻在3个类群中的相对比例最高。其中,甲藻/硅藻比值高值区位于陆坡区,这与大洋水(黑潮)的入侵,带来大量暖水性甲藻有很大关系;颗石藻/硅藻比值总体趋势与生物量的分布相反,在近岸少数站位有高值,向外海逐渐增加,主要是因为颗石藻更适于寡营养盐的环境。生标结果所指示的生物量及群落结构的空间分布与前人的调查结果类似,为利用生标重建此区域的浮游植物生产力和群落结构变化提供了依据。Biomarkers have been widely used to reconstruct phytoplankton productivity based on the assumption that biomarker contents could reflect phytoplankton productivity in the surface seawater.However,this hypothesis has not been validated with modern survey.In this study,the contents and ratios of three phytoplankton biomarkers in the surface seawater of the northern South China Sea in winter 2009 were analyzed,to indicate spatial distributions of phytoplankton biomass and community structure.The results show high values for diatoms,dinoflagellates and coccolithophorids near the Pearl River Estuary and Guangdong coastal areas.The community structure indicates diatoms are the dominant phytoplankton species in winter.High values of dinoflagellate/diatom ratio occur near the slope area owing to intrusion of the Kuroshio,which favors the growth of dinoflagellates.The coccolithophorid/diatom ratio displays an increasing trend from the Pearl River Estuary to offshore region due to the advantage of coccolithophorids in oligotrophic environment,which is opposite to the productivity pattern of all biomarkers.These results are consistent with previous studies using phytoplankton cell and pigments,which provides support for the use of biomarker to reconstruct phytoplankton productivity and community structure in the SCS.国家重点基础研究发展计划项目(2007CB815934、2009CB421201);国家自然科学基金项目(40776029

Generating High-concentration Solution of Reactive Oxygen Species by Strong-field Ionization Discharge

为优化氧活性粒子(rOS)在水中的生成条件,并为rOS溶液生成装置提供优化系统参数的依据,研究了rOS质量浓度在水温、PH值、O2给气体积流量、rOS投加体积质量,以及系统气压(混溶压力)等因素作用下的变化规律。实验中,气态rOS在强电离条件下通过介质阻挡放电生成,以O3计,通过O3检测仪测定其浓度;水中rOS质量浓度采用dPd分光光度法测定,用CrS来表示。实验结果表明:水温、PH值与CrS呈极显著负相关(相关系数P0.05);CrS在水温分别为16℃与24.5℃之间、PH=6.5与PH=7.0之间、O2给气体积流量为2 l/MIn与3 l/MIn之间均为差异不显著(P>0.05),其余各水温、PH值、O2给气体积流量之间均为差异显著(PO2给气体积流量>系统气压与O2给气体积流量交互作用。To optimize the generating condition of reactive oxygen species(ROS) in solution, and to provide a reference for improving ROS preparation system, we investigated the effects of several parameters, including solution temperature, solution pH, O2 input, ROS dosages, and system pressure, on the concentration of obtained ROS solution.Gaseous ROS was generated in a strong-field ionization condition induced by under dielectric barrier discharge(DBD), and its dosage represented by O3 was measured by ozone monitor.ROS in solution was caught by DPD(N, N-diethyl-p-pHenylenediamine), which was measured by DPD spectrophotometry, and the concentration of ROS solution was denoted by CRS.According to the experiments, both water temperature and pH have significant negative correlation with CRS(relativefactor P0.05).The differences between CRS are insignificant(P>0.05) under conditions of solution temperature of 16 ℃, 20 ℃, 24.5 ℃, solution pH of 6.5 and 7.0, as well as O2 input of 2 L/min and 3 L/min(P>0.05), but they are highly significant(P<0.01) or significant(P<0.05) under other tested conditions.Moreover, CRS significantly increases with the decrease of miscibility pressure(P<0.01), and it is significantly affected by the interaction between miscibility pressure and O2 input under higher ROS dosages(P<0.01).Lower water temperature, lower pH, higher ROS dosages, and lower miscibility pressure are all beneficial to increasing the concentration of ROS(CRS), which is significantly affected by the change of several parameters including solution temperature in the lower range, pH around 7, O2 input in the range of larger amount, etc.On the condition of high ROS dosage input, CRS is influenced by miscibility pressure, O2 input, and the interaction between miscibility pressure and O2 input in a descending order.国家高技术研究发展计划(863计划)(2012AA062609); 国家科技支撑计划(2013BAC06B02); 国家杰出青年科学基金(61025001)~