李学麟教授善用桂枝汤的临床举隅

李学麟,福建中医药大学教授,主任医师,被国家中医药管理局授予第一批“全国优秀中医临床人才“的称号。李师幼承庭训,熟读经文,博采众方,首推内经,亦重伤寒,善用桂枝,验之临床,其效桴鼓,今愚揣鄙陋,愿笔下点滴,供同道指正。桂枝汤源自汉代医圣张仲景的《伤寒杂病论》,具辛温解表之功,适用于头痛发热,汗出恶风,鼻鸣干呕,苔白不渴,脉浮缓或浮弱者的外感风寒表虚证。李师在临床实践中,体会桂枝汤,具滋阴和阳,解肌发汗,

Synthesis and Biological Activity of Novel Bis-heterocyclic Compounds Containing 1H-Pyrazole and Thiazole

以苯肼、乙酰乙酸乙酯和氨基杂环为原料 ,合成了两类含双杂环的新型吡唑甲酰胺衍生物 ,其结构经元素分析、1HNMR ,MS及IR确证 .初步的生物活性测试结果表明 :部分化合物对水稻纹枯病菌、小麦赤霉病菌和苹果轮纹病菌具有良好的抑制效果 .In order to search for novel fungicides with high activity and low toxicity, a series of bis-heterocyclic compounds containing 1H-pyrazole and thiazole were designed and synthesized from phenyl hydrazine, ethyl acetoacetate and aromatic aminoheterolcycles. The structures of all new compounds were confirmed by elemental analysis, 1H NMR, IR and MS spectra. Preliminary bioassays indicated that some compounds showed fungicidal activity against Pellicularia sasakii, Gibberella zeave and Physalospora piricola.科技部国家“九五”重点科技攻关 (No .97 563 0 2 0 5)资助项

Effect of NaCl Stress on Physiological Characteristics of Two Manilkara Species Leaves

采用盆栽试验法,对铁线子属果树人心果[MAnIlkArA zAPOTA(l.)VAn rOyEn]和古巴牛乳树[MAnIlkArA rOXburgHIAnA(WIgHT)dubArd]进行nACl胁迫处理,测定叶片的叶绿素、超氧化物歧化酶(SOd)、过氧化物酶(POd)、过氧化氢酶(CAT)、游离脯氨酸、可溶性蛋白等指标及盆土的实际盐度。结果表明,人心果在盐度1.98‰以内、古巴牛乳树在盐度2.42‰以内没有盐害;在nACl胁迫下,两树种的叶绿素合成受到明显抑制;随着处理浓度增加,人心果叶片的SOd活性升高,各处理浓度下的活性都显著高于对照,但POd和CAT活性呈现先升后降趋势,古巴牛乳树叶片的CAT活性升高,但SOd和POd活性降低,三者活性在各处理浓度下都显著高于对照;人心果叶片的脯氨酸含量在各处理浓度均显著高于对照,可溶性蛋白含量在处理浓度为4‰及以上时显著高于对照,古巴牛乳树叶片的脯氨酸、可溶性蛋白在处理浓度为4‰及以上时显著高于对照,推测脯氨酸、可溶性蛋白作为渗透调节物质在人心果、古巴牛乳树受nACl胁迫过程中起着重要的作用。The physiological characteristics of Manilkara zapota(L.)van Royen and Manilkara roxburghiana(Wight)Dubard were studied by watering the potted plants with NaCl solution.The characteristics include chlorophyll,proline,soluble protein,SOD,POD,CAT and so on.The results showed that Manilkara zapota(L.)van Royen grew well below 1.98‰ NaCl stress condition,so did Manilkara roxburghiana(Wight)Dubard below 2.42‰ NaCl stress condition.Under NaCl stress,chlorophyll synthesis was significantly restricted;For Manilkara zapota(L.)van Royen,the activity of the SOD increased,however the activity of POD or CAT increased first and then dropped.The content of proline was higher significantly than that of the control,so did the content of soluble protein when the concentration of NaCl was between 4‰--8‰.For Manilkara roxburghiana(Wight)Dubard,the activity of CAT increased and that of SOD or POD decreased.The activities of the SOD,POD and CAT differed significantly from that of the control.The content of proline or soluble protein was higher than that of the control when the concentration of NaCl was between 4‰--8‰.The small organic molecules working as osmotic potentials in cells played a key role in salt tolerance for the two species.厦门市科技计划项目(No.3502Z20092023

Synthesis and biological activity of novel bis-heterocyclic compounds containing 1H-pyrazole and thiazole

In order to search for novel fungicides with high activity and low toxicity, a series of bis-heterocyclic compounds containing 1H-pyrazole and thiazole were designed and synthesized from phenyl hydrazine, ethyl acetoacetate and aromatic aminoheterolcycles. The structures of all new compounds were confirmed by elemental analysis, H-1 NMR, IR and MS spectra. Preliminary bioassays indicated that some compounds showed fungicidal activity against Pellicularia sasakii, Gibberella zeave and Physalospora piricola

学校流感暴发疫情防控措施动力学模型效果分析

目的探讨学校流感暴发疫情的最佳控制方式,为制定停课标准提供参考。方法通过SEIAR模型对流感暴发疫情隔离和停课效果进行分析。结果本起流感暴发疫情基本再生数R0为7.6180,无干预措施下疫情会持续20天,1 169名学生和教职工（99.74%）会感染流感。第5～10天开始隔离措施,疫情持续时间介于35～44天,第10天开始隔离感染人数是第5天开始隔离的6.9倍。与单独采取隔离措施相比,停课1天、3天、5天、7天可以减少4.51%～10.92%、13.41%～29.31%、19.46%～45.40%和24.82%～57.47%的感染人数,持续时间并没有太大变化;停课14天疫情持续时间都会明显下降,感染人数下降33.43%～65.52%。结论隔离措施越早实施对疫情控制效果越好,显性感染人数达到20%以上时可以停课,出现重症或死亡病例时要将停课时间延长

Detection of point mutations of Axin gene and its expression in gliomas

目的　检测胶质瘤中Axin基因的点突变及其表达情况 ,初步探讨Axin与胶质瘤发生的关系。方法　采用聚合酶链反应 单链构象多态性 (PCR SSCP)技术及DNA测序方法检测Axin基因外显子 8,9及 10在 2 8例胶质瘤中的突变情况 ;同时对上述胶质瘤及正常脑组织进行免疫组化染色。结果　在 2 8例胶质瘤组织中Axin的第 10个外显子共有 6例样本 (2 1.4 % ) 3处发生了错义突变 ;3例 3处发生了同义突变 ;2 8例胶质瘤中 8例 (2 8.6 % )Axin表达阳性 ,正常脑组织中神经元表达阳性 ,神经胶质细胞表达阴性 ,检测到突变的样本中 1例表达阳性。结论　Axin基因的点突变可能参与胶质瘤的发生 【英文摘要】 Objective To detect the point mutations of Axin gene and its expression in glioma and explore the relationship between Axin gene and the occurrence of human glioma.Methods The point mutations of exon 8,9,10 of Axin gene were analyzed in 28 cases of glioma by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) analysis, silver staining and DNA sequencing. Immunohistochemistry was used to detect the Axin expression in these cases and normal brain tissues.Results Three missense poi...高等学校骨干教师计划资助项目;; 归国留学人员科研启动基金资助项目 (1 999747

Accumulation of Rare Earth Elements in Spinach and Soil under Condition of Using REE and Acid Rain Stress

The content and distribution characteristics of REE in spinach and soil under using REE and acid rain stress were studied by pot experiments. The results show that the content of REE is 0.527～0.696 (μg·g - 1) in the above2ground portion of spinach , 21668～31003 (μg·g - 1) in the under2ground portion of spinach and 229109～ 250130 (μg·g - 1) in the soil . With the acidity of acid rain increasing , the leaching of REE in plants and soil is strengthened and the amount of REE reduces with decreasing of pH value . After REE are used , though plants show the selective absorption to Ce group elements (especially spraying on leaves) , regardless under acid rain stress or using REE or not , the distribution model of REE in the above2ground and under2ground portion of plants is basical2 ly the same with the control . Plants also follow the Oddo2Harkins rule of the REE of distribution abundance , light rare earth elements is enriched , the minus of Eu is abnormal and admeasure of Ce is a rich model . The results show that REE in plants mainly come from soil and are affected by it

酸雨胁迫及稀土农用条件下菠菜及其土壤中稀土元素的赋存

利用盆栽实验研究了酸雨胁迫、稀土农用条件下 ,菠菜及其土壤中稀土元素的含量及分布特征。结果表明 :菠菜地上部分的REE含量为 0 .5 2 7～ 0 .696μg·g- 1 之间 ,地下部 2 .668～ 3 .0 0 3 μg·g- 1 。土壤 2 2 9.0 9～ 2 5 0 .3 μg·g- 1 。酸雨明显地影响作物对REE的吸收和利用 ,酸度越大 ,影响越明显。随着酸雨酸度的增大、植株体内、土壤中的REE受淋洗的作用加强而表现出REE的总量随着pH的减小而逐渐减少。施用稀土后 ,植株的地上或地下部分及其土壤中的REE含量均与对照的土壤中的REE分配模式基本相同 ,遵循稀土元素分布丰度的奇偶 (Oddo Harkins)规则、轻稀土富集 ,Eu弱负异常 ,富铈配分型 ,表明稀土元素仍然主要来自土壤并受其影响