采用盆栽试验法,对铁线子属果树人心果[MAnIlkArA zAPOTA(l.)VAn rOyEn]和古巴牛乳树[MAnIlkArA rOXburgHIAnA(WIgHT)dubArd]进行nACl胁迫处理,测定叶片的叶绿素、超氧化物歧化酶(SOd)、过氧化物酶(POd)、过氧化氢酶(CAT)、游离脯氨酸、可溶性蛋白等指标及盆土的实际盐度。结果表明,人心果在盐度1.98‰以内、古巴牛乳树在盐度2.42‰以内没有盐害;在nACl胁迫下,两树种的叶绿素合成受到明显抑制;随着处理浓度增加,人心果叶片的SOd活性升高,各处理浓度下的活性都显著高于对照,但POd和CAT活性呈现先升后降趋势,古巴牛乳树叶片的CAT活性升高,但SOd和POd活性降低,三者活性在各处理浓度下都显著高于对照;人心果叶片的脯氨酸含量在各处理浓度均显著高于对照,可溶性蛋白含量在处理浓度为4‰及以上时显著高于对照,古巴牛乳树叶片的脯氨酸、可溶性蛋白在处理浓度为4‰及以上时显著高于对照,推测脯氨酸、可溶性蛋白作为渗透调节物质在人心果、古巴牛乳树受nACl胁迫过程中起着重要的作用。The physiological characteristics of Manilkara zapota(L.)van Royen and Manilkara roxburghiana(Wight)Dubard were studied by watering the potted plants with NaCl solution.The characteristics include chlorophyll,proline,soluble protein,SOD,POD,CAT and so on.The results showed that Manilkara zapota(L.)van Royen grew well below 1.98‰ NaCl stress condition,so did Manilkara roxburghiana(Wight)Dubard below 2.42‰ NaCl stress condition.Under NaCl stress,chlorophyll synthesis was significantly restricted;For Manilkara zapota(L.)van Royen,the activity of the SOD increased,however the activity of POD or CAT increased first and then dropped.The content of proline was higher significantly than that of the control,so did the content of soluble protein when the concentration of NaCl was between 4‰--8‰.For Manilkara roxburghiana(Wight)Dubard,the activity of CAT increased and that of SOD or POD decreased.The activities of the SOD,POD and CAT differed significantly from that of the control.The content of proline or soluble protein was higher than that of the control when the concentration of NaCl was between 4‰--8‰.The small organic molecules working as osmotic potentials in cells played a key role in salt tolerance for the two species.厦门市科技计划项目(No.3502Z20092023
