提高我国资产管理公司处置不良资产效率的再思考

由厦门大学财政系提供的2003年国有资产监管体制与公共财政制度改革国际会议论文集26我国四大金融资产管理公司在处置银行不良资产方面作出了有目共睹的贡献，但在实践中也存在一些制约因素导致其不能发挥应有的效率。本文认为要提高金融资产管理公司处置银行不良资产的效率，应着力建立支持资产管理公司运作的法律体系；拓宽不良资产处置手段和方式；建立合理的治理结构和激励约束机制，尤其是首次提出要将金融资产管理公司纳入到国有资产管理体系中来

塔里木河下游地下水位对柽柳叶绿素荧光特性的影响

选取塔里木河下游3处地下水埋深>6m的监测井位作为研究点,结合典型生态监测断面的地下水位监测数据,分析不同地下水埋深处柽柳的叶绿素荧光特性和光系统的光合活性.结果表明:随着地下水埋深加大和干旱胁迫加剧,柽柳叶片的实际光化学效率、电子传输速率和光化学猝灭等参数普遍下降;非光化学猝灭和调节性能量耗散量子产量等参数显著升高,而最大光量子产量总体处于相对适宜状态.干旱胁迫下柽柳的PSII光合活性随地下水埋深增大而下降,捕获光能的过剩程度加剧,发生光抑制的几率增大,其自身良好的抗旱性和自我调节机制,使光系统II尚未发生显著光损伤

Essay：Looking back, the world of arts in 1994 回望，94中国美术圈

This essay is scripted in Chinese. The article reviews Zhu Fa-dong’s work ‘Person for sale, price for negotiation’. （Hao Yang 25\u27)https://digital.kenyon.edu/zhoudocs/1290/thumbnail.jp

Extracting condition and the activity admeasurement for pectinesterase in achenes of Ficus awkeotsang Makino

研究了爱玉子果胶酯酶的优化提取方法,并测定了不同条件下爱玉子果胶酯酶的活性及热稳定性。结果表明:爱玉子果胶酯酶抽提液中NaCl的最佳浓度是5%,最佳抽提温度是30℃,最佳抽提时间是24 h,最佳保存时间24 h;酶活测定时作用液中NaCl最适浓度范围为0.6%~1.0%,作用液的最适pH为7.0,温度30~50℃,爱玉子果胶酯酶的热稳定性良好。A method of extraction and assay for the pectinesterase were investigated and proposed as follows: the best concentration of NaCl in extracting mixtrure is 5%,the best extracting temperature is 30℃,the best extracting time is 24 hours,the best conservating time is 24 hours,the best concentration of NaCl in mixture is 0.6% to 1.0%,and maintain the pH on 7.0.Between 30-50℃,the pectinesterase in achenes of Ficus awkeotsang has a good thermal stability.福建省教育厅基金资助项目(K04029);; 福建省科技厅重点资助项目(2007S0015

Preparation of monoclonal antibodies against 3D protein of EV71 based on HBc particles as expression vector

目的:预测肠道病毒71型(EV71)非结构蛋白3D的表位,以HBc蛋白为载体展示多肽,制备并鉴定抗EV71-3D的特异性单克隆抗体(mAb); 。方法:应用生物信息学方法分析预测出EV71; 3D蛋白亲水性和免疫原性指数较高的多肽片段,并运用HBc颗粒型蛋白载体展示肽段,构建多肽融合蛋白,免疫BALB/c雌鼠,通过杂交瘤技术和亲和层析; 技术制备和纯化抗EV71-3D蛋白的特异性mAb,用间接ELISA、ELISPOT、IFA和IHC对mAb的性质进行初步鉴定。结果:构建表达分别; 嵌合3D蛋白34~ 43位氨基酸残基、61~ 76位氨基酸残基、151~; 164位氨基酸残基的HBc重组蛋白,免疫并经过多轮克隆化筛选,获得抗EV71-3D单克隆抗体3E1,其亚类为IgG2a;免疫荧光试验、ELISP; OT法和免疫组织化学染色结果显示其可与EV71特异性结合。结论:成功制备可特异性识别EV71的单克隆抗体3E1,为病毒的检测及进一步研究3D蛋白; 的功能奠定了基础,同时还验证了生物信息学技术与HBc颗粒型载体展示多肽技术相结合可快速高效地制备单克隆抗体。Objective: To prepare and preliminarily identify the monoclonal; antibodies(mAbs) specifically against 3D protein of Enterovirus; 71(EV71),using bioinformatics to predict the epitopes of 3D,with HBc; protein as a carrier.Methods: Artificial screening of 3D protein epitope; sequences by bioinformatic method,inserted into the major immunodominant; region(MIR) area of Hepatitis B virus core protein(HBc),to construct the; recombinant protein.BALB/c mice were immunized with the recombinant; virus like particles(VLPs),to prepare the mAbs against 3D protein of; EV71.Affinity chromatography technology was used to purify the mAb.The; indirect ELISA,ELISPOT,immunofluorescence and immunohistochemistry; staining methods were used to identify the characteristic of the; mAb.Results: We displayed 3D(aa34-43),3D(aa61-76) and 3D(aa151-164); epitopes by constructing fusion protein using HBc VLPs as a vector,after; hybridization,one positive hybridoma cell line(3E1) was selected by; ELISA.The isotype of 3E1 was IgG2a.The results of immunofluorescence and; immunohistochemistry staining assay showed that the mAb 3E1 could; specifically recognize EV71.Conclusion: The prepared mAb 3E1 can; specifically recognizes the EV71,which laid the foundation for the; detection of virus and further study on 3D protein,and verified the; bioinformatics technology combined with HBc carrier displaying peptides; could prepare mAb quickly and efficiently.国家自然科学基金项

什么是好的教育政策研究

为进一步加强教育政策研究,更好服务新时代中国特色社会主义教育事业发展,在教育部-上海市人民政府共建的教育经济宏观政策研究院和华东师范大学国家宏观教育政策研究院的支持下,我们特别邀请全国知名教育政策研究专家围绕\"什么是好的教育政策研究\"展开笔谈。笔谈按作者姓氏笔画排序。华东师范大学国家宏观教育政策研究院委托项目“学界有影响力研究成果评判

监测流动水体污染程度的生物标志物研究进展

利用生物监测流动水体污染程度一直是环境科学研究领域中的热点课题之一。本文综述酶、蛋白细胞和核酸等生物标记物在监测流动水体污染程度方面的研究进展,描述铁蛋白反应器监测流动海水中重金属离子污染的特点,并介绍近年来蛋白组学技术在筛选监测环境污染程度生物标志物中的应用进展

A New Mathematics Model of the Temperature Programmed Analysis Technology

以表面作用包括表面吸/脱附以及表面反应本征速率方程为基础,提出新的程序升温分析技术(TPAT)的数学模型。与经典的理论模型相比,这种新的TPAT理论模型更接近实际的程序升温分析实验过程。设计和进行特定催化剂的TPd、TPr及TPO实验,得到相应的程序升温谱图,采用新的TPAT理论模型模拟上述谱图,计算出相应的表面作用活化能等重要热力学参数。结果表明,新的理论模型具有良好的模拟性能,平均相对误差(Ard)小于1%。Based on the surface effect including surface adsorption/desorption and intrinsic kinetics rate equation,a new mathematical model of the temperature programmed analysis technology was proposed.This model is different from the classical TPAT theory models,which indicates much more coincidence with the actual reactions than other models.TPAT(TPD,TPR and TPO) experiments were designed and carried out to receive their profiles.Based on these profiles of TPAT and the simulated experiments,the novel theory model was designed and the thermodynamics parameters(such as activation energy,etc.) were deduced and calculated by this model.The results show that this model has excellent simulation with the actual experiments,and the average relative errors are easily controlled less than 1%

Effect of Flue-gas Cleaning Devices on Mercury Emission From Coal-fired Boiler

为研究燃煤锅炉烟气净化设施对汞排放特性的影响,采用Ontario-Hydro方法,对设有催化脱硝、静电除尘、海水脱硫的300MW燃煤锅炉排放烟气中汞的含量与形态进行分析,同时测定锅炉的煤、底渣、飞灰等固体样品以及脱硫塔前后、曝气之后海水样品中的汞含量。实验结果为:烟气中的气态汞占总汞的79.1%以上,脱硝催化剂对汞的价态具有强烈的转化作用,烟气中83.4%的气态Hg0被氧化成气态Hg2+;静电除尘对颗粒态汞的去除率几乎达到100%;在脱硫塔中,海水对烟气中汞的洗脱率高达73.6%,曝气后排放前的海水中含汞量是新鲜海水的5.5倍。研究表明锅炉烟气净化设施对汞的排放特性有着重要的影响。In order to study the effect of flue-gas cleaning devices on mercury emission from coal-fired boiler, Ontario-Hydro method had been applied to determine the mercury concentration and speciation in the flue-gas emitted from a 300MW coal-fired boiler, which was equipped with various pollution control devices, including selective catalyst reduction (SCR) De-NOx system, electrostatic precipitator (ESP), and flue-gas seawater De-SO2 system (FGD). Mercury concentration in raw coal, bottom ash and fly ash of the boiler, seawater at the inlet and outlet of SO2 absorption reactor and the drainage of aeration sink, were also analyzed. The results indicate that the percentage of gaseous mercury in total mercury discharged is more than 79.1%. De-NOx catalyst strongly affects the mercury speciation transformation, showing a conversion rate of 83.4% for Hg0 to Hg2+. The removal efficiency of particulate mercury by ESP is close to 100%. With seawater FGD, the removal efficiency of mercury is as high as 73.6%. The mercury concentration in the seawater of drainage from aeration sink is 5.5 times higher than that in fresh seawater. The study shows that the flue-gas cleaning devices in coal-fired power plant play an important role on mercury emission characterization

Atomic structures of enterovirus D68 in complex with two monoclonal antibodies define distinct mechanisms of viral neutralization

11月5日，《自然》子刊《自然•微生物学》（Nature Microbiology）在线刊出了我校夏宁邵教授团队发表的题为“Atomic Structures of Enterovirus D68 in Complex with Two Monoclonal Antibodies Define Distinct Mechanisms of Viral Neutralization”的研究论文。这是夏宁邵教授团队在《自然•通讯》（Nature Communications，2017）、《科学•进展》（Science Advances，2018）上发表手足口病重要病原体CVA6、CVA10研究论文之后的又一项关于肠道病毒的重要研究成果。该研究通过解析肠道病毒D组68型（EV-D68）不同类型病毒颗粒及其免疫复合物的高分辨率结构，系统阐明了EV-D68病毒的生活周期及各时期的病毒中和机制，进一步完善了小RNA病毒的吸附入胞及感染机制理论，为EV-D68新型疫苗、抗病毒治疗药物的研发提供重要的理论指导。该研究依托电镜技术平台，解析了EV-D68病毒生活周期中的三种代表性颗粒成熟颗粒、脱衣壳中间态和前体病毒衣壳的近原子分辨率结构，阐明了三种病毒颗粒间的结构差异，以及成熟颗粒转变为脱衣壳中间态的分子机制。夏宁邵教授、李少伟教授、程通副教授和美国国立卫生研究院（NIH）高级研究员Barney Graham博士为该论文的共同通讯作者。郑清炳工程师、博士生朱瑞、博士后徐龙发、博士生何茂洲和美国加州大学圣地亚哥分校颜晓东博士为该论文共同第一作者。【Abstract】Enterovirus D68 (EV-D68) undergoes structural transformation between mature, cell-entry intermediate (A-particle) and empty forms throughout its life cycle. Structural information for the various forms and antibody-bound capsids will facilitate the development of effective vaccines and therapeutics against EV-D68 infection, which causes childhood respiratory and paralytic diseases worldwide. Here, we report the structures of three EV-D68 capsid states representing the virus at major phases. We further describe two original monoclonal antibodies (15C5 and 11G1) with distinct structurally defined mechanisms for virus neutralization. 15C5 and 11G1 engage the capsid loci at icosahedral three-fold and five-fold axes, respectively. To block viral attachment, 15C5 binds three forms of capsids, and triggers mature virions to transform into A-particles, mimicking engagement by the functional receptor ICAM-5, whereas 11G1 exclusively recognizes the A-particle. Our data provide a structural and molecular explanation for the transition of picornavirus capsid conformations and demonstrate distinct mechanisms for antibody-mediated neutralization.This work was supported by a grant from the National Science and Technology Major Projects for Major New Drugs Innovation and Development (no. 2018ZX09711003-005-003), the National Science and Technology Major Project of Infectious Diseases (no. 2017ZX10304402-002-003), the National Natural Science Foundation of China (no. 81401669 and 81801646) and the Natural Science Foundation of Fujian Province (no. 2015J05073). This work was supported in part by funding by the National Institutes of Health (grants R37-GM33050, GM071940, DE025567 and AI094386). We acknowledge the use of instruments at the Electron Imaging Center for Nanomachines supported by UCLA and by instrumentation grants from the NIH (1S10RR23057 and 1U24GM116792) and NSF (DBI-1338135 and DMR-1548924). 该研究获得了国家自然科学基金、新药创制国家科技重大专项、传染病防治国家科技重大专项和美国国立卫生研究院基金的资助