Construction of Klebsiella oxytoca HP1 Mutants by Homologous Recombination to Enhance Hydrogen Evolution

产氢细菌的产氢能力不高是限制生物制氢技术发展的重要因素。运用分子生物学的手段对菌种进行改造,是提高生物产氢效率的重要方法。乙醇和乳酸是产酸克雷伯氏菌KlebsiellaoxytocaHP1厌氧发酵产H2的主要副产物，切断无益副产物能提高H2产量和底物转化率。本研究以K.oxytocaHP1作为受体，构建了同源双臂整合载体pTA-Str和pLDH-Tr。利用内源平台双交换原理，将质粒pMHE6上携带的氨基葡萄糖苷腺苷转移酶基因（Aminoglycoside-3'-adenyltransferase，aadA）外源表达盒序列（含T7启动子，aadA编码框，T4终止子）定点插入到受体菌细胞染色体乙醇...The low bacterial H2-production ability would be the choke point on the development of bio-hydrogen technology. It is a potential method for enhancing H2-production by using molecular biological technology. Ethanol and lactatic acid are the main byproduct of anaerobic H2-producing fermentation in Klebsiella oxytoca HP1.This can be effectively solved by blocking the pathways leading to the byproduc...学位：工学硕士院系专业：化学化工学院化学工程与生物工程系_生物化工学号：20043301

A Study on Agglomeration Efficiency and Rationality for Manufacturing Industry of the Pan-PRD

产业集聚是指某一产业在某个地理区域集中，同时伴随生产要素在空间上不断集中的过程。改革开放以来，珠三角地区经济发展迅速，成为我国最具战略意义的区域经济之一，制造业部门也不断在广东等地区集聚，促进了珠三角经济增长。随着经济活动集聚程度的加强和区域间交流层次的加深，珠三角地区扩展为今日的泛珠三角地区。但是，制造业集聚也伴随着要素集中和成本上升，可能会削弱由集聚带来的利润增长，因此研究集聚效率及其合理性具有重要的意义。 本文在对产业集聚、规模经济、生产要素拥挤等相关理论研究回顾的基础上，总结了集聚适度和过度的内涵。然后利用各类集聚指标阐述泛珠三角区20个制造业部门的集聚现状和集聚态势。接着利用一系列...Industrial agglomeration refers to a particular industry focusing to a geographical region, along with the factors of production continue to focus on the process. Since the Reform and Opening up, the Pearl River Delta is becoming one of the most important economic regions of China, seeing the rapid development of economics. Manufacturing also continue to gather in Guangdong to promote economic gro...学位：经济学硕士院系专业：经济学院_政治经济学学号：1532011115205

多元化监管制度构建与事业组织公共责任的实现

事业单位是准公共组织,承担着重要的公共责任,其公共责任能否有效实现,有赖于监管制度和机制的构建。我国事业组织存在的失灵失范现象一定程度上是监管制度短缺和监督机制不够健全的产物。为确保事业单位切实履行公共责任,关键在于实施有效且体系化的监管,要将监管法律建设、政府监管、社会监管和事业组织自身监管与行业互律有机结合起来,建立多元一体、系统高效的多元化、多层次监管体系,形成监管合力

Thinking About the Construction of Institutional Repository in Xiamen University Library

目前,如何有效管理不断增多的学术资源且提高学术典藏库利用率,已成为厦门大学学术典藏库急需解决的问题。随着厦门大学科研事业的发展,各学科科研团队作为科研主体,提出了在已建设的厦门大学学术典藏平台基础上,由图书馆学科馆员以科研团队为单位有效管理学术典藏库的意见。因此,本文对厦门大学图书馆学术典藏库建设与服务进行思考,以期能更好地为科研团队提供各种有效的信息服务。Now it's urgent for Xiamen University Institutional Repository(IR) that how to manage the academic resource in an effective way and increase resource utilization rate.With the development of research of Xiamen University, as the main part of scientific research, different kinds of research teams have suggested that the subject librarians should divide resources into several parts based on their teams.Therefore the paper mainly raised the thinking about the constructions of Xiamen University IR to service effectively for research team

学术型图书馆联盟知识服务营销的探索与实践——以开世览文(CASHL)知识服务营销为例

以CASHL设计开发的"特藏++"项目、"名师讲堂"和微信知识服务专栏这三个知识服务产品为例,探讨CASHL知识服务营销在设计营销产品、组织营销主体及选择营销平台这三个方面的特点,提出学术型图书馆联盟在进行知识服务营销时,应围绕知识获取的效率与场景设计知识服务产品、充分挖掘联盟各方力量组织营销主体、考虑多平台和富媒体的知识交付方式,以及重视营销活动的可持续性发展。2017年福建省中青年教师教育科研项目“文献信息资源共享与服务研究专项”“‘双一流'背景下学科知识服务营销体系的理论构建与实证研究”(项目编号:JZ170309)的研究成果之

Klebsiella oxytoca HP1 adhE基因插入失活法构建产氢重组菌

乙醇是产酸克雷伯氏菌(Klebsiella oxytoca)HP1厌氧发酵产H2的主要副产物,每生成1.0 mol的乙醇需要消耗2.0 mol NAD(P)H,从而降低了H2的产量.本研究以编码乙醇脱氢酶系(含乙醛脱氢酶和乙醇脱氢酶活性)的adhE基因为改造目标,利用同源重组技术获得了以提高产氢为目标的K.oxytoca重组菌.构建工作包括:根据adhE基因保守序列框克隆K.oxytoca HP1 adhE基因片段,以质粒pMHE6为模板进行链霉素抗性基因表达盒的扩增,表达链霉素抗性的aadA基因片段和adhE基因片段分别与载体pMD18-T相连构建重组质粒,同源整合质粒pTA-Str的构建,以链霉素作为筛选标记筛选重组菌.菌落PCR鉴定结果表明,aadA基因表达盒通过质粒pTA-Str的介导已定点插入K.oxytoca HP1基因组中,成功地构建了adhE基因部分片段缺失的重组菌.葡萄糖发酵实验结果表明,相同发酵条件下,重组菌比野生菌的产氢量提高了16.07%,乙醇产量下降了70.47%.利用基因工程技术提高产氢初步获得成功.国家自然科学基金(批准号:30470395);; 福建省重点科技项目(批准号:2005I106

Cloning and expression analysis of lipopolysaccharide-induced TNF-α(LITAF) of Japanese scallop (Mizuhopecten yessoensis)

脂多糖诱导的肿瘤坏死因子(lIPOPOlySACCHArIdE-InduCEd Tnf-AlPHA fACTOr,lITAf)是一类重要的炎症细胞因子,在先天性免疫系统中发挥重要的介质作用。文章根据虾夷扇贝lITAf基因EST序列,应用rACE技术克隆了虾夷扇贝lITAf全长CdnA,对序列及编码的氨基酸进行生物信息学分析。结果显示,该基因CdnA全长1 551 bP,其5′非编码区包含76 bP,3′非编码区包含1 001 bP;开放阅读框(Orf)为474 bP,编码157个氨基酸,氨基酸序列中存在一个保守的lITAf结构域;理论分子量16.99 kdA,等电点为6.24。lITAf基因序列为3 698 bP,由3个外显子和两个内含子组成。利用实时荧光定量PCr技术分析lITAf在虾夷扇贝不同组织、不同胚胎发育阶段以及鳗弧菌(VIbrIO AnguIllAruM)刺激后各时间段的表达情况。结果表明:lITAf基因在所检测的6个成体组织中均有表达,其中肾脏的表达量最高;胚胎发育的7个时期中,担轮幼体时期表达量最高;菌刺激36 H实验组与对照组的表达量差异大。lITAf基因是lITAf家族的一员,推测lITAf基因参与虾夷扇贝的先天性免疫反应。The lipopolysaccharide-induced TNF-alpha factor(LITAF) is an inflammatory cytokine,which plays an important role in innate immunity system.Based on the expressed sequence tag(EST) of Japanese scallop(Mizuhopecten yessoensis),the cDNA of LITAF gene was amplified using rapid amplification of cDNA ends(RACE) approach.Results showed that the full-length cDNA of LITAF is 1 551 bp consisting of a 5′ untranslated region(UTR) of 76 bp,a 3' UTR of 1 001 bp,and an open reading frame(ORF) of 474 bp encoding a polypeptide of 157 amino acids,and there is a conserved LITAF domain in amino acid sequences.The estimated molecular mass is 16.99 kDa and the theoretical isoelectric point is 6.24.The total length of LITAF is 3 698 bp,which includes three exons and two introns.Real-time quantitative PCR was carried out to measure LITAF mRNA expression in adult tissues and monitor mRNA expression patterns during embryonic development after bacteria(Vibrio anguillarum) challenged.The expression level of LITAF mRNA was detected in all the adult tissues with the highest in the kidneys.The trochophore owns the highest expression level of LITAF in embryonic development.LITAF expression showed significant difference(P<0.01)between the control and bacteria challenged specimens at 36 h.These results suggest that the LITAF should be a member of the LITAF family that perhaps involved in the innate immune response of Japanese scallop.国家海洋公益性行业科研专项(编号:200805037);国家自然科学基金项目(编号:31140073)资

Functional Data Mining: Application in the Analysis of Chinese Consumption Function

以数据挖掘的思想,提出了利用Bernstein基构建一般函数数据的方法。在此基础上,根据中国31个省(自治区、直辖市)城镇居民的人均年收入和消费性支出的数据,构建了消费函数数据,并进行误差分析,求出消费函数的一阶和二阶导数,进一步挖掘消费函数的发展速率,取得良好的效果。Based on the idea of data mining,a method of using Bernstein bases to construct general functional data is given in this article.With the data of urban per capita annual income and consumptive expenditure in 31 provinces(Autonomous regions,Municipalities) of China,consumption functional data is constructed and error analysis is done based on this study.In order to further mining the developing velocity of consumption function,first and second order derivatives of the function are computed and good effect achieved.教育部“新世纪优秀人才支持计划项目”《数据挖掘中最新统计方法研究》(NCET-04-0608);; 教育部社科研究规划项目《数据挖掘中关联规则的统计研究及应用》(06JA91003