TGF-β1 induces activation of HSC-T6 cells and epithelial-mesenchymal transition in rats

目的探讨转化生长因子-β1（TGF-β1）在大鼠肝星状细胞系（HSC-T6）活化及上皮间质转换（EMT）中的作用。方法体外培养HSC-T6，用MTT法筛选TGF-β1对HSC-T6作用的最佳浓度；用10μg／L TGF-β1处理HSC-T624h，相差倒置显微镜下观察细胞形态改变，免疫荧光染色法检测细胞骨架结构F-actin蛋白的表达，RT-qPCR法检测肌动蛋白α-SMA及代表上皮间质转换的神经黏附素（N-cadherin）、波形蛋白（vimentin）和上皮黏附素（E-cadherin）基因表达；用不同浓度（0、5和10μg/L）的TGF-β1处理HSC-T624h，Western blot检测α-SMA、N-cadherin、vimentin和E-cadherin蛋白表达。结果10μg/L TGF-β1干预HSC-T624h有最好的细胞存活率；TGF-β1刺激HSC-T6后，细胞拉伸，伪足增多呈星形，细胞间连接疏松，呈显著活化状态；F-actin聚集形成应力纤维丝，沿细胞长轴分布；实验组α-SMA mRNA及vimentin mRNA的表达量明显高于对照组（P〈0．05），而E-cadherin mRNA的表达量明显降低（P〈0．05）；在不同浓度的TGF-β1呈剂量依赖性致α-SMA及N-cadherin和vimentin的蛋白表达量增多，而E-cadherin的蛋白表达量减少。结论TGF-β1可诱导HSC-T6活化及上皮间质转换。Objective To observe the effect of TGF-β1 on activation and epithelial mesenchymal transition （EMT） in rat hepatic stellate cell-T6. Methods Adopt the MTT method to screen the optimum concentration of TGF-β1 in vitro HSC-T6 cultured. After the HSC-T6 stimulation by TGF-β1 of 10 μg/L for 24 hours, the morphology of the cells was observed under inverted phase contrast microscope, the expression of F-actin which on behalf of cotoskeletal structure was detected by immunofluorescence staining; the expression of α-SMA and N-cadherin, vimentin, E-eadherin was measured by RT-qPCR ; The changes of α-SMA, N-cadherin, vimentin and E-eadherin were assessed by Western blot after different concentrations （0,5 and 10 μg/L） of TGF-β1 interventing HSC-T6 for 24 h. Results The optimal cell survival rate was recorded when 10 μg/L TGF-β1 dealt with cells for 24 h. After HSC-T6 were treated with TGF-β1 ,cells stretched, pseudopodia increased and turn into stellate, ceils connections were looser, so that represented a significantly activated state. F-actin filaments gathered to form stress and distributed along the long axis of the cells ; The expression of α-SMA mRNA and vimentin mRNA in experimental group was significantly higher while E-cadherin mRNA was obviously lower than the control group （P〈 0. 05）. TGF-β1 made the protein expression of α-SMA and N-cadherin, vimentin in dose-dependent increased while E-cadherin was decreased.Conclusions TGF-β1 may induce activation and epithelial-mesenchymal transition of HSC-T6.福建省科技计划（2015Y01010224）;福州市科技计划（2014-S-137-1

Mechanisms and Prevention of Skin Photoaging

皮肤老化是一个复杂、多因素所致的过程,其结果是皮肤弹性和功能发生改变。引起皮肤老化的原因包括内源性(如年龄等)和外源性(如紫外线等)因素,后者所致的皮肤老化即皮肤光老化。本文以国外文献为基础,着重介绍皮肤光老化的发生机制,描述与之相关的主要信号通路,总结皮肤光老化的预防措施,以指导进一步的研究。Skin aging is a very complicated process with many factors involved, which will lead to changes in skin elasticity and functions.Skin aging can be triggered by both intrinsic(e.g., age) and extrinsic(e.g., ultraviolet light) factors.The latter is also called skin photoaging.This paper reviewed the mechanisms of photoaging and related prevention approaches based on international literature.It also described the major signaling pathways involved in the process of photoaging so as to inform further studies.国家自然科学基金青年项目(编号:81101562); 广东省科技计划项目(编号:2012B060300005); 广东省自然科学基金面上项目(编号:S2012010009633

Advances for the Ruthenium Complexes-Based Homogeneous Catalytic Hydrogenation of Oxalates to Ethylene Glycol

草酸酯加氢是重要的有机化学反应,在工业制乙二醇生产中有着重要的应用.对钌金属配合物均相催化反应的研究进行了综述.以催化反应体系为焦点,探讨了多种; 因素如温度、氢气压力、催化剂浓度、反应时间、添加剂等对底物转化率以及产物选择性的影响,并讨论了催化反应机理,其中金属-配体协同促进的H_2分子异; 裂,进而完成对底物分子中酯基依次加氢的反应机理是探讨的重点,这为新型催化剂的设计和应用提供参考.Hydrogenation of oxalates is one of the important organic reactions, which has an ultimate use for the industrial production of ethylene glycol. The studies on the ruthenium complexes-based homogeneous catalytic reaction systems are herein summarized. With the focus on the catalytic reaction systems, the important factors with significant influences on the oxalate transformation efficiency as well as the product selectivity are discussed, including temperature, H-2 pressure, catalyst concentration, reaction duration, additives, and so on. The catalytic reaction mechanisms are also discussed in detail, where the mechanism for the H-2-heterolysis promoted under the metal-ligand cooperation for the oxalate hydrogenation to ethylene glycol is enhanced. This study would be useful for designing the new catalyst applicable in industry.National Natural Science Foundation of China [21473142, 91545115,; 21473145]; Innovative Research Team of China [IRT_14R31, J1310024

D-loop Sequence Variation of Mitochondrial DNA in Captive Chinese Alligator

扬子鳄 (Alligatorsinensis)是中国特有的珍稀爬行动物 ,至 2 0 0 0年 ,野生扬子鳄的个体数已不足 15 0条 ,作为保护这一物种的措施之一 ,先后于 80年代初建起了 2个养殖场 ,现人工繁殖的扬子鳄总数已达 90 0 0余条。为揭示扬子鳄种群遗传多样性 ,从两个饲养种群中采集了 42个个体的样品 ,其中宣州样品 33个 (XZSP) ,长兴样品 9个(CXSP) ,用PCR方法扩增mtDNA控制区 ,扩增产物纯化后直接用ABI 310全自动遗传分析仪荧光标记测序 ,得到其中 39个个体的mtDNA控制区 5′端 46 2bp的序列。经比对发现 ,39个个体间的 5′端mtDNA控制区没有任何变异位点 ,共享一种单元型 ,提示扬子鳄饲养种群的遗传多样性非常贫乏 ,造成这一结果的主要原因是近 5 0年来 ,扬子鳄种群衰退和数量迅速减少导致的遗传多样性丢失 ,其次是人工繁殖的群体同时受到始创者数量较少产生的瓶颈效应影响。针对扬子鳄遗传多样性的现状 ,作者最后就这一濒危动物遗传多样性的保护对策提出 3点建议。Chinese alligator,Alligator sinensis,is a critically endangered endemic species under legislative protection in China.Result of recent investigations revealed that number of the alligators was continuously declining in the past 50 years and less than 150 individuals were surviving in the wild until 2000 years.In order to prevent the extinguishing of this species,two breeding farms were set up in early 1980s at Xuanzhou county,Anhui Province and Changxing County, Zhejiang Province respectively.After twenty years of breeding efforts,the number of captive individuals has been brought up to more than 9 000 in total, forming two separate captive subpopulations,Xuanzhou subpopulation (XZSP) and Changxing subpopulation (CXSP).Because of lack of the information regarding genetic diversity of the captive populations, 42 captive individuals including 33 individuals from XZSP and 9 from CXSP were sampled randomly to investigate their genetic status for the strategy in the next protection action.PCR method was adopted for amplification of mitochodrial DNA control region using primers designed in this research.After purification of PCR products,all of amplicons were sequenced directly with ABI BigDye TM Terminator Cycle Sequencing Ready Reaction Kit and ABI 310 genetic analyzer.Consequently, 5′end of control region with length of 462 base pair was obtained from 39 samples.Sequence alignment shows there is no any variation site in this range of control region among the individuals assayed here, namely only one haplotype of the region shared by these alligators.This result strongly indicates that the population of captive Chinese alligator is in very poor genetic diversity status.Reasons for the losing of genetic diversity in the population are mainly attributed to population depression and number of individual decreasing sharply in the past 50 years.Another factor accounting for the phenomena is the limit of founder number of captive population.Finally,authors proposed three pieces of advice for the genetic conservation of Chinese alligator.教育部骨干教师资助计划项目 (GG -180 - 2 10 0 2 40 3 -1740 );; 江苏省“333工程”人才培养基金资助~

Spatial sampling method for crop acreage based on remote sensing in hilly area in Minhou County,Fujian Province

以福建闽侯县作为研究区,采用传统抽样(简单随机抽样、系统抽样、分层抽样); 、空间抽样(空间随机抽样、空间系统抽样、空间分层抽样)等方法对研究区农作物种植面积进行样本抽选、总体推算及误差估计,结果表明: 1 500; m*1 500; m格网为最优抽样单元尺寸,空间分层抽样方法的相对误差为3.86%,变异系数为6.03%,抽样成本为6.03,抽样效率高.与传统抽样方法相比,空间; 抽样方法显著减少样本容量,节约调查成本.Traditional sampling methods of simple random sampling,systematic; sampling,stratified sampling,and spatial sampling methods of spatial; random sampling,spatial system sampling and spatial stratified sampling; were applied to conduct sample extraction,population extrapolation and; error estimation in crop acreage in Minhou County. The results showed; that spatial stratified sampling had the highest sampling efficiency; with 1 500 m*1 500 m square grid as the optimal sampling unit size. And; relative error,variation coefficient and sampling cost was 3.86%,6.03%; and 6.03,respectively. Compared to traditional sampling method,spatial; sampling method significantly reduced sampling size and saved cost.全国统计科学研究项

FICM干预对急性运动大鼠抗氧化能力的影响

目的:探讨远红外陶瓷微珠对大鼠一次性力竭运动后血清SOD、GSH-Px、MDA含量的影响。方法:将88只雄性SD大鼠分为安静对照组(A组)、模型组(B组)、模型+热水组(C组)、(模型+陶瓷微珠组D组)。B、C、D组大鼠利用下坡跑跑台一次性运动至力竭。各组大鼠分别于安静时与运动后即刻、24h、48h、72h取心脏血,分离血清测定SOD、GSH-Px、MDA含量。结果:D组运动后各时相血清SOD、GSH-Px含量均高于B组与C组同时相(P <0. 05),D组运动后各时相血清MDA含量均低于B组与C组同时相(P <0. 05)。结论:陶瓷微珠干预手段具有抑制急性运动后机体氧自由基生成,减少过氧化损伤的作用。福建省科技厅省自然科学基金项目(2016J01404);;莆田学院育苗基金项目(2017014);莆田学院国家基金预研项目(2016076);;国家体育总局科技服务项目(2017HT008

间歇式轴向压应力对组织工程骨种子细胞的黏附增殖与成骨分化促进作用的研究

目的探究间歇式轴向压应力对组织工程骨种子细胞黏附、增殖与成骨分化能力的影响。方法构建表达绿色荧光蛋白的兔骨髓间充质干细胞(rBMSCs)作为示踪种子细胞,运用旋转细胞培养仪将松质骨支架和种子细胞共培养7 d获得组织工程骨(TEB),实验组在第7～14天施加大小10 N、频率1 Hz、4 h/d的间歇式轴向压应力刺激,对照组常规培养,14 d后胰酶消化法获取两组种子细胞并比较其黏附、增殖和成骨分化能力。采用两组独立样本t检验进行统计学分析。结果 (1)流式细胞术显示rBMSCs被成功提取分离。(2)倒置荧光显微镜及扫描电镜显示TEB中种子细胞与支架相容性良好。(3)活体荧光成像系统及扫描电镜显示应力刺激组种子细胞的生长状况要优于非应力刺激组,前者平均荧光密度及细胞数/500倍视野均大于后者,差异均具有统计学意义(平均荧光密度:(3.75±0.34)×108 vs(2.91±0.22)×108,t=2.90,P=0.04;细胞数/500倍视野:30.50±4.43 vs 21.00±5.13,t=3.14,P=0.01)。(4)细胞黏附实验显示,应力刺激组种子细胞的75﹪细胞贴壁时间短于非应力刺激组,两组时间分别为(3.00±0.41) h、(13.33±1.70)h,差异具有统计学意义(t=8.20,P <0.01),前者的最终细胞贴壁率高于后者(99.97﹪±0.34﹪vs 85.83﹪±1.18﹪),差异具有统计学意义(t=11.31,P <0.01)。(5) CCK-8检测显示,在培养第48～96 h,应力刺激组种子细胞的增殖能力优于非应力刺激组,将两者的450 nm吸光度值在第48小时(0.49±0.02、0.40±0.02)、72 h(0.76±0.07、0.64±0.04)和96 h(1.58±0.07、1.34±0.13)分别进行比较,差异均具有统计学意义(t=5.15、2.57、2.86,P均<0.01)。(6)在成骨诱导14 d后,应力刺激组种子细胞的ALP和Ca结节染色阳性率要强于非应力刺激组:两组ALP染色阳性率分别为26.73﹪±4.56﹪、16.68﹪±3.89﹪,差异具有统计学意义(t=3.33,P=0.03);两组Ca结节染色阳性率分别为41.81﹪±3.56﹪、27.40﹪±2.35﹪,差异具有统计学意义(t=3.68,P=0.02)。结论间歇性轴向压应力可促进组织工程骨种子细胞的黏附、增殖与成骨分化

远红外陶瓷微珠干预对急性损伤大鼠LZM、MPO、IL-8和IL-10指标影响的研究

利用远红外陶瓷微珠作为干预手段,对大鼠肌肉急性损伤后进行恢复治疗,探讨其对骨骼肌血清炎症指标LZM、MPO和免疫学IL-10、IL-8指标的影响.将88只SD雄性大鼠分为A组安静对照组; B组模型组; C组模型+热水组; D组模型+陶瓷微珠组,大鼠急性损伤造模成功后自然休息48 h后,各组实验大鼠按照设计分别取干预后第3 d、4 d、6 d、7 d时相点数据进行组内、组间分析.结果:(1)干预后第3 d,D组血LZM、IL-10恢复后各时相水平均显著高于自然恢复B组与模型C组(P <0. 05);(2)干预后7 d,D组血MPO、IL-8恢复水平均显著低于自然恢复B组与模型C组(P <0. 01).结论:(1)远红外陶瓷微珠干预下,发现降低了MPO水平而提升了LZM水平,说明干预后急性损伤炎性细胞对损伤肌肉的破坏进一步减小;(2)远红外陶瓷微珠干预可提升IL-10浓度水平和降低IL-8的浓度水平,验证其影响炎症反应过程,促进急性损伤的快速恢复.福建省自然科学基金资助项目(2016J01404);;莆田学院育苗基金资助项目(2017014);莆田学院国家基金预研资助项目(2016076);;国家体育总局科技服务资助项目(2017HT008

旋转玻碳电极上二茂铁的电化学阻抗行为及其与DNA的相互作用

应用旋转圆盘电极和电化学阻抗法研究了二茂铁在Tris-NaC l(pH=7.2)缓冲溶液中于旋转玻碳电极上的电化学阻抗行为及其与DNA的相互作用.结果表明,二茂铁于旋转电极的伏安曲线呈现明显的极限电流平阶,而其交流阻抗谱则出现两个电容弧.二茂铁与DNA的作用,若受扩散过程控制则其极限扩散电流随DNA浓度增大而减小,而在电化学控制过程中则表现为电化学反应电阻随DNA浓度增大而增大.根据旋转圆盘电极和电化学阻抗谱测试,表明由这两种方法数据拟合求得的二茂铁条件电位速率常数能够很好地相互吻合,但如存在DNA时,则其条件电位速率常数有一定程度的减小

Discussion on the Participation of Infantile Massage in Community Health Care

小儿推拿具有取穴少、经济安全、操作简单、家长易学等优点,适合在社区中推广。本文就其参与社区卫生服务的方式、内容、优势、问题和解决方法等方面,对实现小儿推拿“家庭化“进行论述,认为小儿推拿参与社区卫生服务最理想的方式是实现其“家庭化“,且“家庭化“的建立将是医疗技术进入社区的重要措施,是家庭卫生服务中最具特色的形式之一。Infantile massage has many advantages,for example,less acupoint selection,economic,safe,simple operation and easy to learn.It's suitable for promoting in the community.From aspects of methods,details,advantages,problems and solutions of infantile massage participating in community health service,the paper discussed infantile massage "family oriented"and thought that achieving its "family oriented"was the best way of infantile massage participating in community health service.The building of its"family oriented"was the key of medical technology entering into the community,and was one of the most unique forms of the family health service