Preparation of Monoclonal Antibody against Stevioside and Development of ELISA Method for Stevioside

甜菊苷(stevioside，ST)为甜叶菊中所含的主要甜味成分，是理想的高甜度、低热量，对糖尿病和高血糖脂有很好疗效的一种新型高安全性甜味剂，也可作为一些药品的品位改良剂和矫味剂，从而广泛应用于食品和药物。目前，甜菊叶中甜菊苷的提取工艺已经日趋成熟，然而甜菊苷的准确含量的检测方法主要是使用HPLC，还存在不足之处，因此，建立简单，快速，准确的甜菊苷定量方法对于甜菊苷的生产和应用都具有极其重要的意义。 本研究利用高碘酸纳法将甜菊苷（ST）分别与人血清白蛋白（HSA）和牛血清白蛋白（BSA）进行偶联，成功制备了免疫抗原和包被抗原，经MALDI-TOFMS鉴定得HSA，BSA上分别挂13，8个S...Stevioside, as the major sweet ingredient of Stevia rebaudiana, is a new kind of natural high sweetness, low-calorie and high safety sweetener, which has physiological functions including anti-hyperglycemia and anti-diabetes. It’s also can be used as improving the tastes of medicine. Its stable chemical and physical properties and non-fermentability lead to long shelf life and widely using in food...学位：理学硕士院系专业：生命科学学院_生物化学与分子生物学学号：2162007115201

Analysis of the Approaches of the Feminist Critique of Science

女性主义以“性别分析“为核心展开其科学批判与科学研究,在传统科学观、建构论与科学实践论三种视域中形成三条不同的研究进路:(1)在传统科学观视域中,女性主义主要以“概念分析“为进路,试图通过对理性、客观性等概念的男性中心主义批判,揭示科学中的性别歧视与男性气质,并以女性气质、女性主义新客观性等概念构建其理论(;2)在建构论视域中,女性主义以“理论建构“为进路,试图通过对建构表征与反身性困境的批判和反思来构建女性主义科学衍射新模式与科学主客体交互关系理论;(3)在科学实践论视域中,女性主义以“异质性因素的动态分析“为其进路,使其科学研究视域转向性别、种族、阶级等异质性因素与科学的动态作用研究,揭示性别差异的多层次性与科学文化的多样性。The feminists conduct their critique of science and scientific research focused on gender analysis.In the following three domains, that is, in the traditional view of science, in constructivism, and in the scientific practice theory, three different approaches have formed: (1) In the perspective of the traditional view of science, the feminists mainly appeal to the approach of ìthe conceptual analysisī.It attempts to criticize such androcentric concepts as rationality and objectivity, and then reveal the gender discrimination and the masculinity in science, and construct its theory with the femininity and the new objectivity of feminist concept; (2) In t constructivism, the feminists appeal to the approaches of ìthe theoretical constructionsī, attempt to construct the new feminist mode of science diffraction and the theory of host-guest interaction of science by the critique and reflection on the dilemmas of construction representation and reflexivity.(3) In the scientific practice theory, the feminists appeal to the approaches of ìthe dynamic analysis of the heterogeneity factorī, turn to the research into such heterogeneity factors as gender, race, class, etc, and the dynamic role of science, and reveal the multi-level differences of gender and the diversity of scientific culture.教育部人文社会科学研究青年基金项目:技术与性别:女性主义技术哲学研究(12YJC720049

From Gender Culture of Science to Diversity of Science Culture——the Science Culture from the Perspective of Feminism

传统女性主义从性别维度分析传统科学文化,揭示出传统科学文化中存在的性别歧视与科学的“菲勒斯中心主义“。受后现代主义与后殖民主义理论的影响,新时期女性主义解构传统科学文化的一元结构,从阶级、民族、地域等方面坚持科学文化的多元立场,女性主义科学文化观从科学的性别文化转向了科学文化的多样性研究。后现代主义对女性身份的消解与后殖民主义对差异性与多元性的强调为女性主义多元文化发展带来新动力与巨大挑战,唯有建立一种使后现代主义的解构、多元主义的差异与女性主义批判完美结合的女性主义文化,女性主义多元文化才能继续发展。From the perspective of feminism,science culture changes with the development of feminism.Based on gender analysis,traditional feminism holds that there are gender discrimination and "Phallus Centrism" of science culture views in traditional science culture.With the development of feminism,the new feminism deconstructs traditional monistical science culture under the influence of postmodernism and postcolonialism,holding that science culture is plural in such aspects as classes,nations,regions and so on.Thus,the study of science culture views turn from gender culture of science to diversity of science culture.There is doubtless that the study will keep developing with the inspiration of new theories in future

基于微纳制造的第三代基因测序系统的基础理论研究年度报告

本研究属于微纳制造与生命科学的交叉领域，面向第三代基因测序系统的研制，系统地开展微纳制造、微纳流体动力学和超灵敏单分子检测的基础理论、关键问题的研究，研制出具有自主知识产权的第三代基因测序仪的原型系统，推动我国科研重大装备的国产化进程。本研究2013年度在以下三方面取得重要进展： （1）协同利用多种微纳制造和跨尺度制造手段（双束系统、高分辨电镜、原子层沉积和毛细管拉伸系统等），针对多种不同材料（氧化铝、氮化硼和有机生物孔）采用多种手段（压力驱动、复合结构和纳米电极设计）开展纳米孔器件制造和DNA 高灵敏检测研究，在硅基和碳基纳米孔的轴向和径向尺寸可控制造方面取得重要突破。 （2）深入探讨DNA 过纳米孔的关键问题离子电流的形成与调控机理以及DNA 分子与壁面的特异性作用，进一步发展了粗粒化分子动力学模拟模型，开展跨尺度流体动力学建模和计算，并且利用单分子力谱直接探测DNA的单分子界面摩擦力。对于DNA等生物分子在受限空间内受力的科学认识，为DNA分子过孔测序技术的发展提供理论依据和设计指导。 （3）综合运用生物分子在纳通道中的输运过程和规律，发展纳通道中生物分子信号的超灵敏检测方法与原理。研制出基于金属场效应管的纳通道单分子传感器，建立多模式检测的实验方案，实现检测信号的多元化，提高检测容量，增强检测体系的分辩率与区分度

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies