Experimental effects study of total flavonoids of Ajuga on nuclear transcriptional factor of kappa B in mesangial proliferative glomerulonephritis rats

目的探讨筋骨草总黄酮(TfA)治疗系膜增生性肾小球肾炎(MSPgn)大鼠后核转录因子-κb(nf-κb)及下游转化生长因子-β1(Tgf-β1)的变化。方法采用改良慢性血清病MSPgn大鼠模型,于造模第5周末检测尿蛋白,将尿蛋白阳性者随机分为模型组、雷公藤多甙(TWP)组、不同剂量TfA组。于造模第6周开始给药,6周后,测定各组大鼠的24 H尿蛋白定量、血生化和肾组织病理变化,MAXVISIOnTM即用型快速免疫组化一步法检测大鼠肾组织中nf-κb P65的表达,双抗体夹心法检测血清中Tgf-β1的变化。结果 TfA可显著降低尿蛋白,明显减小系膜区面积占毛细血管丛面积的百分比(P0.05)。模型对照组大鼠肾组织nf-κb P65、血清Tgf-β1的表达明显高于正常对照组[nf-κb P65:28.93±4.45 VS.4.51±0.96,P0.05).The renal tissue NF-κB(Integral optical density, IOD) and serum TGF-β1(pg/ml) in model control was significantly higher than that in normal control group, NF-κB p65 28.93±4.45 vs.4.51±0.96, P<0.01; TGF-β1:(247.63±51.55)pg/ml vs.(127.52±27.84)pg/ml, P<0.01; With the intervention trerapy of high-dosage TFA, middle-dosage TFA and TWP, the expression of NF-κB in renal tissue and TGF-β1 in serum was significantly decreased compared with model control group, NF-κB p65: 10.35±2.81, 15.21±4.71,11.70±3.97, P<0.01; TGF-β1:(181.96±52.93)pg/ml,(204.33±38.30)pg/ml,(188.07±42.92)pg/ml, P<0.05.Conclusion Reducing the expression of NF-κB and successive TGF-β1 may be one of the mechanisms of total flavonoids of Ajuga treatment on MsPGN rats.福建省卫生厅中医药科研重点课题(wzzsb0906); 陈可冀中西医结合发展基金·福建省中西医结合老年性疾病重点实验室开放课题资助项目(CKJ2008088); 福建中医药大学校管课题(X2009069

Experimental Effects Study of Total Flavonoids of Ajuga on Interleukin-1 and Tumor Necrosis Factor-α in Mesangial Proliferative Glomerulonephritis Rats

目的:探讨筋骨草总黄酮对系膜增生性肾小球肾炎(MSPgn)大鼠的干预作用机制。方法:采用改良慢性血清病MSPgn大鼠模型,于造模第5W末检测尿蛋白,将尿蛋白阳性者随机分为模型组、雷公藤多甙(TWP)组、不同剂量筋骨草总黄酮(TfA)组,另设正常对照组。于造模第6W起开始给药,6W后,测定各组大鼠的24 H尿蛋白定量、血生化和肾组织病理变化,放射免疫分析法观察TfA对MSPgn模型大鼠血清中白细胞介素-1(Il-1)、肿瘤坏死因子-α(Tnf-α)的影响。结果:TfA可显著降低尿蛋白,明显减小系膜区面积占毛细血管丛面积的百分比、减轻肾脏损害(P0.05)。模型对照组大鼠血清中Il-1(ng.Ml-1)、Tnf-α(ng.Ml-1)的表达明显高于正常对照组(Il-1 0.829±0.251 VS0.238±0.081,P0.05)。结论:筋骨草总黄酮对MSPgn大鼠有较好的治疗作用,降低Il-1和Tnf-α的水平,可能是其治疗作用机制之一。Objective:To investigate the effects of total flavonoids of Ajuga(TFA) in mesangial proliferative glomerulonephritis(MsPGN) rats and its mechanism.Methods:MsPGN rat was induced by the improved chronic serum sickness model after five weeks.The rats with positive urinary protein rats were randomly divided into model group,triptergium wilfordii polyglycosidium(TWP) group and TFA group with different dosage,normal rats wasere considered as the control group.MsPGN rats were treated with drugs since the sixth week;six weeks after succussfully modelingeeding six weeks later,all rats were detected 24 h urinary protein,and blood biochemistry,pathomorphological change in renal tissue,serum Interleukin-1(IL-1) and tumor necrosis factor-α(TNF-α) wasere measured by radioimmunoassay.Results:Compared with model group,TFA can significantly reduce the urinary protein,decrease BUN,Scr,TG,Tch,mitigate the morphological damage,and significantly increase TP(P0.05).The serum levels of IL-1 and TNF-α(ng·mL-1) in model control wasere significantly higher than that in normal control group;.With the intervention trherapy of high-dosage TFA,middle-dosage TFA and TWP,the expressions of serum IL-1 and TNF-α wasere significantly decreased compared with model control group(.The expressions of IL-1 and TNF-α in high-dosage TFA,and middle-dosage TFA groups wasere slightly higher than those in TWP group,with no significant differences(P>0.05).Conclusion:Total flavonoids of Ajuga has a therapeutic effect on MsPGN rats;Rreducing the expressions of serum IL-1 and TNF-α,which may be one of the mechanisms of MsPGN treatment.福建省卫生厅中医药科研重点课题资助项目(wzzsb0906); 陈可冀中西医结合发展基金·福建省中西医结合老年性疾病重点实验室开放课题资助项目(CKJ2008088); 福建中医药大学校管课题资助项目(X2009069

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研究了铁锰复合氧化物(fMbO)吸附去除AS(Ⅲ)、AS(V)的性能。结果表明fMbO对AS(Ⅲ)、AS(V)均具有较好的吸附能力,其饱和吸附量分别为111.10、71.40 Mg·g-1。AS(Ⅲ)和AS(V)是通过与fMbO表面的fE—OH基团进行交换并形成内层络合物的形式被fMbO吸附,且AS(Ⅲ)的吸附是吸附和氧化共同作用的结果。另外,沼液中共存离子对AS(Ⅲ)和AS(V)的吸附有不同的影响。zn2+能够增加fMbO对AS(Ⅲ)、AS(V)的吸附量,且增加幅度随着zn2+浓度的增加而增加;磷酸根对AS(Ⅲ)、AS(V)的吸附有明显的抑制作用,当磷与砷的分子摩尔比为1时,fMbO对AS(Ⅲ)、AS(V)的吸附量分别降低了34.70%、31.50%;但是有机物(腐殖酸、动物蛋白及尿素)对fMbO吸附AS(Ⅲ)、AS(V)的影响不大。利用fMbO对实际沼液中的砷进行吸附,结果表明砷的去除率平均达到65%左右,使吸附后某些沼液中砷的浓度达到生活饮用水标准和地表水排放标准。因此,将fMbO用于砷污染的沼液及水体的治理具有很好的应用前景。The adsorption behavior of arsenite(As(Ⅲ)) and arsenate(As(Ⅴ)) by Fe-Mn binary oxide(FMBO) was studied.The results indicated that FMBO had strong adsorption ability to both As(Ⅲ) and As(Ⅴ) and the maximum adsorption capacity was 111.10, 71.40 mg·g-1 respectively.As(Ⅲ) and As(Ⅴ) were adsorbed on FMBO surface through forming inner-sphere surface complexes by ligand exchange with hydroxyl groups, and As(Ⅲ) removal by FMBO was through an oxidation and adsorption combined process.In addition, the influences of co-existing substances generally present in biogas slurry were examined.Zinc ion could promote As(Ⅲ) and As(Ⅴ) adsorption on FMBO and the adsorption capacity increased with increasing zinc ion concentration.Phosphate had significant effect on As(Ⅲ) and As(Ⅴ) removal.When P/As ratio was equal to 1, the adsorption capacity of As(Ⅲ) and As(Ⅴ) was reduced by 34.70%, 31.50%, respectively.However, organics, such as humic acid, animal protein and carbamide had no significant effect on As(Ⅲ) and As(Ⅴ) removal.Moreover, FMBO as adsorbent for removal arsenic of actual biogas slurry was investigated.The average removal rate of arsenic of actual biogas slurry was about 65%, decreasing the arsenic concentration of some biogas slurry to the drinking water and surface water discharge standard.Therefore, FMBO could be an attractive adsorbent for both As(Ⅴ) and As(Ⅲ) removal from biogas slurry.国家重点基础研究发展计划项目(2013CB733505); 国家自然科学基金项目(21077086)~

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