Degradation of 2-methylisoborneol in water by hydroxyl radical

二-甲基异莰醇(2-MIB)是一种由蓝绿藻以及放线菌等微生物产生,具有桥环结构的饱和叔醇,在水中具有令人厌恶的土霉味,常规水处理工艺难以对其氧化; 降解.利用大气压强电离放电生成羟基自由基(·OH),对2-MIB进行氧化降解,确定了其氧化剂剂量效应、时间效应关系,并利用GC-MS对2-MIB; 氧化降解过程中间产物的分析,推断其氧化降解机制.结果表明:对初始浓度为150,300ng/L的2-MIB,分别投加总氧化剂TRO1.8,2.3m; g/L,接触反应6s去除率分别为96%和97.6%,处理后残余浓度低于10ng/L; (低于人类嗅阈值).在2-MIB水样中加入·OH淬灭剂叔丁醇(TBA)后,2-MIB的去除效果明显降低,证明氧化降解2-MIB的主要为·OH.另; 外通过对氧化降解过程中间产物分析表明,·OH能破坏2-MIB的桥环结构,并最终矿化生成CO_2和H_2O.2-methylisoborneol (2-MIB) produced by cyanobacteria and actinomycetes; is a saturated bicyclic-tertiary alcohol, which can cause earthy/musty; taste and odor in surface water. Moreover, 2-MIB is usually difficult to; be decomposed and removed by conventional water treatment process. In; this paper, hydroxyl radical (·OH) generated by a strong ionization; discharge process at atmosphere pressure was used to degrade 2-MIB in; water, of which the removal efficiency including dose effects and; contact reaction time were investigated. The intermediate products; formed in ·OH treatment process were analyzed by GC-MS, and the; oxidative degradation mechanism of 2-MIB by ·OH was discussed. Results; show that the removal rate for 2-MIB with initial concentration of 150; and 300ng/L could reach 96% and 97.6% within 6.0s, while the total; reactive oxidant (TRO) dose were 1.8 and 2.3mg/L, respectively. After; ·OH treatment, the concentration of 2-MIB in water was lower than 10ng/L; (lower than the human olfactory threshold). The degradation effects of; 2-MIB were obviously reduced by the ·OH scavengers tertiary butyl; alcohol (TBA), indicating that ·OH should be the main oxidant for 2-MIB; oxidative degradation. By analyzing the intermediates produced in the; oxidative degradation process, it was found that the bridge ring; structures of 2-MIB could be destroyed by ·OH and finally mineralized to; CO_2 and H_2O.国家科技支撑计划项目; 国家重大科研仪器研制项目; 科技部创新人才推进计划重点领域创新团队; 辽宁省重点实验室基础研究项

文蛤多肽粉对人肝癌细胞SMMC-7721的抑制作用及免疫调节作用

将文蛤肉以复合胰蛋白酶酶解,经喷雾干燥工艺得到了文蛤多肽粉,研究文蛤多肽粉对肝癌细胞的抑制作用和对昆明种小鼠的急性毒理作用和免疫调节作用,结果显示文蛤多肽粉对体外培养的肝癌细胞SMMC-7721具有明显的杀伤作用,使细胞变形以至破裂从而抑制癌细胞的生长,抗肿瘤作用明显;文蛤多肽粉给药组的胸腺指数和脾脏指数较空白对照组明显升高,提示文蛤多肽粉能促进小鼠胸腺和脾脏的生长发育,增强免疫力

300kg/a规模流化床制碳纳米管中试研究

采用Ni-Mg-O复合氧化物催化剂进行了流化床甲烷催化裂解法制碳纳米管的中试实验,研究了主要操作变量对甲烷转化率、催化剂产碳率、产品团聚率及催化剂损失率的影响,得到了适宜的操作条件为:甲烷进气流速16～19 cm/s、催化剂粒径150～220μm、催化剂加入量50～60 g、反应温度650～700℃、反应时间120～140 min。多批次重复性实验表明,在选定的操作条件下,甲烷转化率约为30%,催化剂产碳率约为10 gCNTs/gCAT。对纯化后的产品进行SEM及TEM形貌表征显示,制得的碳纳米管管径均匀,中空结构明显,碳纳米管的外径为10～30 nm,内径为2～5 nm。中国海洋石油总公司科技项目(CNOOC-KJ125FZDXM00TJY001-2014

聚苯胺对纳米CdS的光致发光增强效应

利用电化学脉冲沉积法在聚苯胺 ( PANI)膜上制备了纳米 Cd S/PANI复合膜 ,并利用扫描电镜光谱、紫外可见光谱、红外光谱、拉曼和荧光等光谱技术表征复合膜的形貌、结构及性质 .Cd S/PANI复合膜中Cd S微粒呈现量子尺寸效应 ;Cd S和 PANI间存在相互作用 ;由于聚苯胺和 Cd S能级的合适匹配 ,聚苯胺对Cd S的光致发光 ( PL)有增强效应 ,增强机理为光生载流子的传递机

苦参碱对人食管癌细胞株Eca-109增殖和凋亡的影响

目的探讨中药提取物苦参碱单体(Matrine)对人食管癌细胞株Eca-109的诱导凋亡和抑制增殖作用。方法体外培养人食管癌细胞株(Eca-109),分别给以不同浓度的苦参碱对体外培养的Eca-109细胞株进行干预。以MTT比色法、Hoechst33342染色法以及流式细胞术测定苦参碱对Eca-109细胞株的诱导凋亡及抑制增殖的作用。结果 MTT实验显示苦参碱可以明显抑制Eca-109细胞株的增殖,流式细胞术检测细胞周期显示G2期细胞明显增多,S期细胞显著减少。结论苦参碱能明显抑制Eca-109细胞株的增殖,促进其凋亡,其作用机制可能与细胞周期阻滞于G2期有关

食管癌组织中CuZn-SOD活性和表达的研究

[目的]通过对食管癌组织和癌旁组织中CuZn-SOD活性和表达检测,探讨CuZn-SOD活性和表达在食管癌发生、发展中的作用。[方法]CuZn-SOD活性和表达的检测分别采用DTNB直接法和Western Bloting方法。[结果]总SOD和CuZn-SOD的活性在食管癌组织和相应癌旁组织中的差异尚不能认为有统计学意义。30%食管癌组织中CuZn-SOD的表达明显高于癌旁组织。[结论]不同病例中表达水平结果不一致,CuZn-SOD的表达水平与肿瘤的发生或发展存在一定的联系

聚苯胺纳米管在阳极氧化铝模板中电聚合的生长机理

利用阶跃电位、循环伏安等电化学方法,应用金属电沉积理论,研究聚苯胺纳米管在阳极氧化铝模板(AAO)内电聚合的生长机理.结果表明:电聚合初始,苯胺二维非扩散控制的瞬时成核生长形成聚苯胺晶胞链段单层,单层形成以后,聚苯胺的生长过程变为线性扩散控制.XRD实验进一步证实电聚合的聚苯胺同时包含结晶相和无定型相结构

The effect of elemene on reversal multidurg resistance and the expression of P-glyco-protein in A549/DDP

目的:探讨榄香烯乳(ElEMEnE,ElE)逆转人肺腺癌A549/ddP细胞株耐药性及其机制。方法:采用MTT法检测榄香烯乳单用的细胞毒作用及与顺铂(CISPlATIn,ddP)合用时耐药逆转作用;采用流式细胞术检测榄香烯乳对A549/ddP细胞内罗丹明-123(rHOdAMInE-123,rH123)蓄积的影响;采用WESTErn blOT检测榄香烯乳对耐药细胞A549/ddP细胞膜上P-gP蛋白表达的影响。结果:不同浓度榄香烯对A549/ddP细胞均有一定的抑制作用,呈时间-剂量依赖性效应。单用ddP的IC50为15.46μg/Ml,合用20μg/Ml榄香烯24H,IC50为4.15μg/Ml,逆转耐药倍数为3.63。同时,榄香烯增加A549/ddP细胞内rH123的蓄集,降低细胞膜上PgP的表达,且呈剂量依赖性,表明榄香烯能减少A5 4 9/ddP细胞对药物的外排和抑制P-gP蛋白的表达。结论:榄香烯在体外逆转肿瘤细胞耐药性可能与其抑制P-gP的功能和表达有关。Objective: To investigate the elemene( ELE) reversal of human lung adenocarcinoma A549/DDP cell resistance and its mechanism.Methods: Cytotoxicity of ELE alone or in combination with DDP in A549 / DDP cells was determined by MTT assay.Based on flow cytometric technology,the effect of ELE on the uptake of rhodamine-1 2 3( Rho1 2 3) in A5 4 9 / DDP cells was detected by measuring Rho123 mean fluorescence intensity( MFI).P- gp protein expression on the A549 / DDP cell membrane were detected by Western blot.Results: Different concentration elemene inhibited the A549 / DDP cell proliferation in a time- dose- dependent manner( P < 0.05).Alonely used DDP,IC 50 was 15.46μg / ml; combined 20μg / ml elemene for 24h,DDP IC 50 was 4.15μg / ml,the reversal activity was 3.63 times higher than that of DDP.Elemene increased intracellular accumulation of Rh123 of the A549 / DDP cells, reduced the expression of P- gp on the cell membrane,in a dose- dependent manner,indicating that elemene reduce A549 / DDP cells for drug efflux and inhibition of P- gp protein pump function.Conclusion: The reversal activity of DDP is possible related to inhibition of P- gp function and expression in vitro,which lead to an increased intracellular accumulation of anticancer drugs.福建省自然科学基金面上项目(编号:2010D014

黑龙江省扎赉特旗牛羊胰阔盘吸虫病流行病学及病原生物学的研究

一、前言 牛羊胰脏吸虫病是我国各农牧区中常见而且危害严重的一种吸虫病,国内此吸虫病的病原主要有胰阔盘吸虫(Eurytrema pancreaticum(Janson)、腔阔盘吸虫(E.coelomaticum(Giard et Billet)及枝睾阔盘吸虫(E.cladorchis Chin,Li et Wei)三种,其中以胰阔盘吸虫的分布区最大。关于本类吸虫的生物学问题近年来经国内外蠕虫学者的探讨而逐渐被了解(唐仲璋,1950;唐仲璋、唐崇惕,1975、1977;Basch,1965;Ksembaeva,1967;1973等)。国内在福建已详细观察了腔阔盘吸虫和枝睾阔盘吸虫生活史全过程,并经试验证实了它们的贝类宿主及不同的昆虫宿主的种类。胰阔盘吸虫的生活史虽亦经阐明,