乙醇对斜生栅藻叶绿素荧光特性以及油脂积累的影响研究

为了研究乙醇对斜生栅藻（Scenedesmus obliquus XJ002）藻株光合效率以及胞内油脂积累的影响,首先采用酶标仪作为检测仪器,研究了尼罗红终浓度、二甲基亚砜（DMSO）体积分数、尼罗红染色时间对藻株油脂测定的影响,分析了藻株胞内油脂含量与尼罗红染色相对荧光信号强度之间的线性关系,为XJ002藻株胞内油脂含量的测定提供了一个简便、快速的方法.其次研究了不同体积分数的乙醇添加对XJ002藻株生物量、叶绿素荧光特性以及胞内总脂含量的影响.结果表明:XJ002藻细胞尼罗红染色最佳条件是:二甲基亚砜体积分数为2%,尼罗红的终质量浓度为2.0μg·m L-1,尼罗红的染色时间为5 min.添加高浓度乙醇导致藻细胞生物量与光合色素含量显著下降,影响了藻细胞光系统Ⅱ反应中心能量分配,抑制光系统Ⅱ反应中心受体侧电子传递活性.当乙醇处理浓度为3%时,XJ002藻株胞内总脂含量较对照组提高了5.19%,该研究表明乙醇可以作为XJ002藻株油脂积累的潜在诱导试剂.国家自然科学基金项目(41666007);;内蒙古自治区自然科学基金项目(2015MS0335);;包头市应用研发资金科技计划项目(2015Z2010-3

The Historical and Realistic Interaction between Puyuma and The Neighboring Ethnic Groups in Taiwan

如今的卑南族是台湾第六大原住民族群，其聚落主要分布在台湾东部的山海平原之间。自古以来，与卑南族毗邻并且互动较多的原住民族群有阿美族、排湾族、鲁凯族和布农族，这几个族群在相当长的历史时期内共享台东地区的资源，日渐形成一种自然状态下的较为稳定的族群关系格局。然而，随着汉人族群在台湾的扩张，以及台湾数次被外来殖民者统治的特殊历史发展轨迹，使得台东地区以卑南族为主体的原住民族群互动呈现出更为复杂的样貌。 由于无文字民族历史记忆的传递以口碑传说为主要方式，因此考察卑南族与周边族群的互动唯有从荷领台湾时期文字记载增加后方才渐次明晰，卑南等族原住民也正是从这个时候开始以更加鲜明的形象进入历史的视野。自此以...Pinuyumayian is the sixth largest indigenous group in Taiwan, whose settlements mainly scatter on the plains and mountains in the eastern part of Taiwan. Since ancient times, there are Amis, Paiwan, Rukai and Bunun living around with the Puyuma, who have shared resources in Taitung area for quite a long time and gradually formed a relatively stable pattern of ethnic relations under the natural sta...学位：法学硕士院系专业：人文学院_中国少数民族史学号：1062013115236

全球治理语境下主权国家的国际责任

全球治理理论强调国际事务的多元主体共治,但并不否认主权国家仍在其中扮演着主导性角色,尤其是在承担国际责任方面的作用更是无可替代。倡导主权国家的国际责任不仅有利于维护其自身日益扩展的利益需要,而且也有利于维护世界的和平与发展。尽管面临着一些困境,但无论在经济、政治、生态还是安全领域,主权国家都应该克服困难,践行自己的国际责任。崛起中的中国应当坚持力所能及的均衡责任观,塑造负责任的大国形象,有选择性地担当国际责任。国家社会科学基金项目《转型期的中国政治稳定机制研究》(11BZZ018); 江苏省高校优势学科建设工程项目《中国和平崛起的国际环境研究》(1812200001A174)的研究成

代谢中间物对斜生栅藻叶绿素荧光特性以及油脂积累的研究

为了研究代谢中间物(柠檬酸与苹果酸)的添加对斜生栅藻(Scenedesmus obliquus XJ002)光合效率以及胞内油脂积累的影响,研究了代谢中间物的添加对XJ002藻株生物量、光合色素、叶绿素荧光特性以及胞内总脂含量的影响。结果表明:添加高浓度代谢中间物导致藻细胞生物量与光合色素含量显著下降,损坏藻细胞光系统II反应中心供体侧的放氧复合体(OEC)结构,抑制光系统II反应中心受体侧电子传递活性。当柠檬酸与苹果酸处理浓度为0.15 g/L时,XJ002藻株胞内总脂含量是对照组的1.85倍和1.34倍。高浓度代谢中间物的添加,显著提高了XJ002藻株胞内总脂含量,与苹果酸相比,柠檬酸的添加效果更佳。国家自然科学基金(41666007);;内蒙古自治区科技计划项目(201601061);;内蒙古科技大学大学生创新基

Using of Wet Process to Remove of Heavy Metals from Waste Incineration Fly Ash

以福建和湖北垃圾焚烧发电厂采集的飞灰为研究对象,比较了单独水洗、水洗结合酸洗等工艺对飞灰重金属的洗脱效果,考察了不同酸洗工艺条件下飞灰重金属浸出毒性的变化。结果表明:在水灰比为10时水洗效果最佳,水洗结合酸洗处理可有效降低飞灰重金属浸出毒性,部分飞灰经湿法脱除后可作为普通废物排放。Taking the fly ash samples obtained from Fujian and Hubei waste incineration plants as the research subjects,the effects of water washing and wash+acid washing for heavy metal extraction from fly ash were compared.The changes of leachimg toxicity of heavy metals from fly ash were investigated under different acid washing conditions.The results showed that the effect of water washing was best when the ratio of water to fly ash was 10 ∶ 1(L/kg).Water+acid washing process can effectively reduce the leachimg toxicity of heavy metals.Part of fly ash can be disposed as ordinary waste after treated by wetextraction process

Identification of hepatitis C or/and G virus RNA in one tube by reverse transcription nested polymerase chain reaction

目的庚型肝炎病毒（HgV）与丙型肝炎病毒（HCV）同属黄病毒科，且传播途径相似，重叠感染率高，本研究旨在建立一种同时检测HgV与HCV感染的方法。方法根据HCV与HgV的基因序列分别选取5’-uTr（HCV）与nS3（HgV）的两套引物，在同一管内进行逆转录-巢式聚合酶链反应，并初步应用于153例标本。结果该方法能同时检出HgV与HCV感染，扩增片段大小与设计相符。结论该方法简便特异，适用于临床检测Hepatitis C and G viruses belong to the flaviviridae family.They have similar modes of transmission.The dual infection of HGV and HCV were reported.A simultaneous detection for HCV or/and HGV was established by reverse transcription nested polymerase chain reaction(RT-nested PCR).The primers were derived from the 5'non-coding region of HCV and NS3 region of HGV ,and the length of the PCR product was between 170bp and 300bp.RT-nested PCR can be finished in one test tube and HGV and /or HCV can be detected simultaneously in one RT-nest PCR.153 samples were detected by his method.Our results showed that the established assay is useful for screening and identification of HCV or/and HGV RNA.This method is simple and specific practicable.国家九五课

中国河南两株庚型肝炎病毒(HGV)NS5区部分cDNA的克隆与序列分析

通过逆转录－聚合酶链反应从我国河南省２例重叠感染ＨＣＶ或ＨＢＶ／ＨＤＶ的献血员中，分离到ＨＧＶＮＳ５区的部分ｃＤＮＡ，对其进行序列分析比较，结果表明：河南株ＨＧＶＮＳ５区核苷酸与两株中国ＨＧＶ株的同源性（＞９１．９％）高于国外代表株（８８．５％－９０．６％），但由核苷酸推导的氨基酸的同源性都无明显的地区性区别。ＨＧＶＮＳ５区氨基酸序列较保守（同源性＞９１％），缺乏明显高变区。中国４株ＨＧＶ在７３８４位发生了由Ｃ→Ｔ的变异，从而导致一个共同的保守位点的产生（由Ｆ→Ａ）。重叠感染其他肝炎病毒时，河南株ＨＧＶＮＳ５基因结构并无特异性改

第十八届美国理论与应用力学大会总结

1会议概况2018年6月5—9日,第18届美国理论与应用力学大会(18th U.S. National Congress of Theoretical and Applied Mechanics, USNCTAM2018)在美国芝加哥召开.本次大会由美国力学国家委员会和中国力学学会联合主办,旨在探讨和交流近四年世界范围内在理论和应用力学领域的基础研究、创新技术的最新进展,吸引了来自世界各地的近千名专家学

Analysis on the epidemiological characteristics of the sporadic cases with hepatitis E infection in the rural area in Jiangsu province

目的了解江苏省农村散发性戊型肝炎的流行病学特征。方法通过建立覆盖市、镇、村三级医疗卫生服务机构的疑似肝炎主动监测网络,系统全面地监测戊型肝炎病例的发病情况。结果主动监测网络的敏感性明显高于网络报告系统,能更加准确、全面地掌握戊肝的发病规律。结果显示戊型肝炎病例占疑似急性肝炎病例的26.7%,男性戊肝发病率高于女性(P<0.01);发病随着年龄增长而上升,多见35岁以上人群;全年均有发病,冬春季节较高;戊型肝炎病毒株HEV1、4型并存,但以HEV4型为主(92.5%)。结论疑似肝炎主动监测系统数据显示目前戊肝发病率有被低估的风险。To understand the epidemiological characteristics of the sporadic cases with hepatitis E infection in the rural area of Jiangsu province,the prevalence of hepatitis E infection was surveyed systemically and completely through the initiatively monitoring system for the suspected cases of hepatitis E covering the city,town and village levels of medical and health service organizations.It was found that the initiatively monitoring system for suspected hepatitis E cases reflected the incidence of hepatitis E more accurately and comprehensively and was proved to be more sensitive than the network reporting system.The cases with hepatitis E accounted to 26.7% of the suspected cases with acute hepatitis and they were more frequent in male than in female.The incidence of hepatitis E increased with advance of age and most of cases occurred with age over 35.Estimated incidence was stable on the whole year,but rose up in winter and spring.The HEV type 1 and type 4 co-existed,but the HEV type 4 was proved to be the main type(92.5%).From the above observations,it seems that the initiatively monitoring system for the suspected cases of hepatitis E may be under-estimated for the risk of the hepatitis E infection in rural area of Jiangsu province.国家高技术研究发展计划(863计划)(2006AA02A209)资