A study of the influence of film-formation additive and surface coating on the performance of Lithium Nickel Manganese Cathode Material

摘要 随着能源和环境问题的日益凸显，锂离子电池作为新的储能方式越来越广泛地应用在电动汽车(EV)，混合动力电动汽车(HEV)和插入式混合动力电动汽车(PHEV)等领域。为了获得更高的能量密度和功率密度，开发高电压正极材料如5V尖晶石LiNi0.5Mn1.5O4（LNMO）和与之相关的基础研究成为锂离子电池研究的重要方向之一。然而，造成LNMO容量衰减的因素主要有两个方面：LNMO的工作电压超出了电解液的电化学窗口（Abstract With the energy and environmental issues becoming increasingly serious, Lithium-ion batteries (LIBs) are now widely used in portable electronic devices, hybrid electric vehicles (HEVs), plug-in hybrid electric vehicles (PHEVs) and pure electric vehicles (PEVs), etc.. The application of high-voltage cathode material like LiMn1.5Ni0.5O4 (LNMO) with a 4.7 V voltage platform has been conside...学位：理学硕士院系专业：化学化工学院_物理化学学号：2052012115156

A Study on Development Strategies of Shunfun Chain Co.,Ltd.

[]于波，范从来.我国先进制造业发展战略的PEST嵌入式SWOT分析[J].南京社会科学，2011(7):34-40. []牛晓帆.西方产业组织理论的演化与新发展[J].经济研究，2004(3):116-123. []刘长军.基于产业组织理论的我国装备制造业产业竞争力研究[D].哈尔滨工业大学，2008. []刘益.波特“五力模型”的缺陷及其改进[J].管理工程学报，1999(S1). []张光明，吴燕.基于波特五力模型的我国造船产业结构分析[J].江苏科技大学学报:社会科学版，2009，9(1):58-61. []龚小军.作为战略研究一般分析方法的SWOT分析[J].西安电子科技大...无学位：工商管理硕士院系专业：管理学院_工商管理硕士(高级管理人员工商管理硕士)学号：X201415636

Research on Automated Batch Cells Injection System Based on Visual Control

细胞注射作为基本的生物微操作技术，在转基因、克隆、人工受精、医药研究等方面发挥着重要作用。当前，细胞注射主要以手动或者半自动化的注射方式来完成，但是细胞注射的高度技巧性、复杂性和不稳定性使手动和半自动化注射方式效率低下、重复性差。本文研究一套能够自动批量完成细胞注射的系统，以提高细胞的注射效率和成功率，减轻操作人员的负担。本文的主要工作有以下几个方面： 1．细胞自动批量注射系统的综述。对细胞注射在生物研究中的作用和现有细胞注射系统以及采用的技术进行综述，引出细胞批量注射系统的研究意义。 2．细胞批量注射系统的体系结构。介绍系统中各个设备的性能参数以及细胞批量固定芯片的设计和制作。 3．细...As a basic technology of bio-micromanipulation, cells injection plays an important role in many research fields, such as transgenics, clonning, artificial insemination, drug development, and so on. Currently, the cells injection is mainly implemented manually or semi-automatically, which results in low efficiency and poor reproducibility, because of cell injection's high technique, complexity, and...学位：工学硕士院系专业：物理与机电工程学院机电工程系_机械电子工程学号：1992007115118

检测细胞角蛋白在食管癌外周血和骨髓微转移中的意义

目的:探讨食管癌患者外周血和骨髓中CK18mRNA基因表达及临床意义。方法:采用巢式RT-PCR方法检测50例M0、10例M1的食管癌患者及10例食管良性病变患者外周血及肋骨骨髓中细胞角蛋白CK18mRNA基因的表达。结果:50例M0食管癌患者外周血、骨髓中CK18mRNA阳性表达率分别为38.0%(19/50)和42.0%(21/50);10例M1的食管癌患者外周血、骨髓中CK18mRNA阳性表达率分别为90.0%(9/10)、100%(10/10);食管良性病变患者的外周血和骨髓中K18mRNA阳性表达率均为0%(0/10),CK18mRNA阳性表达在三组间差异有显著性(P0.05),但呈正相关性(r=0.704,P<0.01)。结论:应用RT-PCR法检测食管癌患者外周血或骨髓中CK18mRNA微转移有助于食管癌的分期和预后的判断

Expression profile of voltage-gated potassium channel Kv1.3 in aorta of atherosclerosis rats

目的探讨动脉粥样硬化(AS)大鼠主动脉病变局部离子通道kV1.3的表达水平及作用。方法雄性WISTAr大鼠16只,随机分为两组:正常组(8只,予普通饮食+0.9%氯化钠溶液)和AS组(8只,予高脂饮食+维生素d3负荷)。采用组织病理学检查,观察主动脉粥样硬化病变。采用实时定量反转录-聚合酶链反应(rT-PCr)和WESTErn印迹法检测主动脉病变局部kV1.3和白细胞介素(Il)-2、干扰素(Ifn)-γ的表达水平。结果组织病理学检查证实纤维增生性AS斑块。AS组的kV1.3MrnA为(31.48±8.64)x10-3,显著高于正常组的(3.28±0.79)x10-3(P=0.012)。AS组的kV1.3、Il-2、Ifn-γ蛋白表达量分别为0.691±0.067、0.611±0.055、0.759±0.050,均显著高于正常组的0.490±0.052、0.299±0.058、0.273±0.052(P值均<0.01)。结论 kV1.3在主动脉粥样硬化病变局部的表达增高。kV1.3可能在AS的发生和发展过程中发挥着重要的作用。Objective To investigate the expression of voltage-gated potassium channel Kv1.3 in the aorta of a rat model of atherosclerosis and its role in the progress of atherosclerotic plaque formation.Methods A total of 16 male Wistar rats were randomly divided into normal control(normal diet and saline)and atherosclerosis group(high lipid diet+Vitamin D3 overload),with 8 rats in each group.In 16 weeks later,all rats were killed after weighing,and their blood samples and aorta were collected.Pathological changes of the rat aortic artery were observed with HE staining.Real time RT-PCR and Western blotting analysis were used to determine the mRNA and protein expression of Kv1.3 and the protein expressions of interleukin(IL)-2 and interfereron(IFN)-γ.Results Pathological changes showed that fiber proliferative atherosclerotic plaques were found in the aorta of atherosclerosis group,with inflammatory cells infiltrating in the local lesion.Real time RT-PCR analysis showed that the expression of Kv1.3 mRNA in the aorta was increased significantly in the atherosclerosis rats than that in the controls([31.48±8.64]×10-3 vs.[3.28±0.79]×10-3,P<0.05).Western blotting analysis showed that the protein expression of Kv1.3,IL-2 and IFN-γ in the aorta were also increased significantly in the atherosclerotic rats than that in the controls(Kv1.3[0.691±0.067] vs.[0.490±0.052],IL-2 [0.611±0.055] vs.[0.299±0.058],IFN-γ [0.759±0.050] vs.[0.273±0.052],P<0.01 n=8).Conclusion The expression of Kv1.3 potassium channels is increased in the plaques of atherosclerotic rats.Kv1.3 may play an important role in the development and progression of atherosclerosis.国家自然科学基金资助项目(30871045

Prevalence of hepatitis B virus infection and phylogenetic analysis of HBV S gene among high school students

目的了解江苏省海门市高中生乙型肝炎感染情况及其HbV S基因的变异情况。方法采用整群抽样方法抽取江苏省海门市职业中心校的407名高三学生进行乙肝血清学调查;采用酶联免疫吸附法(ElISA)和巢氏聚合酶链式反应(PCr)扩增方法检测血清样本的乙型肝炎表面抗原(HbSAg)、乙型肝炎病毒表面抗体(抗-HbS)和乙型肝炎病毒(HbV)S基因并进行进化分析。结果 407名高中生中乙肝表面抗原(HbSAg)阳性率为6.39%;在家中、乡镇卫生院和县级及以上医院出生学生的HbSAg阳性率分别为11.76%、8.02%和2.27%,差异有统计学意义(P<0.05);抗-HbS滴度≥10u/l者占72.5%,且呈u形分布;不同乡镇、不同性别学生抗-HbS滴度差异有统计学意义(P<0.01);407名高中生HbV S基因均为C基因型,主要变异在蛋白疏水区,抗原决定簇A区未发生免疫原性改变。结论出生时接受的医疗水平对HbV感染有影响;海门市高中生HbV S基因受到正选择作用而出现变异,但未发生免疫原性改变。Objective To understand the hepatitis B infection and HBV S gene variation among high school students in Haimen city,Jiangsu province.Methods A serological survey of hepatitis B was conducted in a high school with cluster sampling method.HBsAg and anti-HBs were detected with ELISA,and phylogenetic analysis for HBV S gene with nested PCR.Results HBsAg positive rate was 6.39% and the rate for the students born at home,township health centers,and hospital above the county level was 11.76%,8.02%,and 2.27%,respectively,with significant difference(P<0.05).The percentage of the students with anti-HBs titer ≥ 10U/l was 72.5%,and the titer distribution was U-shaped.The difference in anti-HBs titer between boys and girls,as well as different towns,was statistically significant(P<0.01).All genotype of HBV S gene was C-type,and the main variation was in protein hydrophobic area.The change of immunogenicity in antigenic determinant "a" zone was not observed.Conclusion The level of health care at birth can affect the HBV infection among high school students in Haimen.S gene variation occurs through positive selection,but not antigenicity.国家科技支撑计划(2006BAI02A03);传染病重大专项(2008ZX10002-012);上海市重点学科项目(B118

献血者中戊型肝炎病毒血症研究

目的　了解湖州地区献血者戊型肝炎病毒感染情况。方法　采用ELISA法检测 30 4 7名无偿献血者血清抗 HEVIgM和IgG抗体 ,巢式RT PCR检测抗 HEVIgM阳性血清中HEVRNA。 结果　 30 4 7名无偿献血者中抗 HEVIgG的检出率为 4 1.7% ,抗 HEVIgM检出率为 1.5 %。 4 6份抗 HEVIgM阳性中发现 6份HEVRNA阳性。献血者中病毒血症阳性率为 0 .2 %。 6名HEV病毒血症者中 4名为HEV基因Ⅰ型 ,另 2例为HEV基因Ⅳ型。结论　在献血者中HEV病毒血症并不罕见 ,有必要对输血后戊肝的潜在危险进行评估