Classification method of diabetes based on integration of characteristic classifier

目的:结合医用电子鼻技术,探讨糖尿病患者及其口腔呼气的气味图谱特征。方法:选择180例糖尿病患者和100例健康者,用医用电子鼻采集280例口腔呼气的气味图谱,采用基于数据特征划分的方法,用支持向量机和随机森林集成模型对糖尿病患者进行分类预测。结果:1线性核函数的支持向量机(SVM1)分类结果不是很理想,低于多项式核(SVM2)、径向基函数核(SVM3)和随机森林(RF)3种分类器,说明分类超平面显然是非线性的;2集成分类器对糖尿病患者和健康者的气味图谱特征的识别准确率可达88.04%。结论:基于特征划分的分类器集成方法预测性能明显好于单一分类器,为使用医用电子鼻进行糖尿病诊断分析提供了一种有效手段。Objective: To discuss the proi le features of oral odor of diabetic patients based on medical electronic nose technology. Methods: 180 patients of diabetes and 100 healthy people were selected, and the proi le features of oral odor of 280 volunteers were collected by using medical electronic nose. The classii cation forecasting was carried out on diabetic patients by using support vector machine(SVM) and random forest integration model based on partitioning method of data characteristics. Results: 1The classii cation result of SVM1 was not very good, which was lower than that of SVM2, SVM3 and RF, and the result showed that the classii cation hyperplane is nonlinear. 2The accurate rate of recognition of integrated classii er on diabetic patients and healthy people is 88.04%. Conclusion: The forecasting performance of classii er integration method based on feature division is superior to that of single classii er signii cantly, which provided an ef ective means for the diagnostic analysis of diabetes based on medical electronic nose.国家自然科学基金项目(No.81373552);; 福建省教育厅A类项目(No.JA14212);; 福建工程学院科研启动项目(No.GY-Z12079)~

Effect of Exogenous Nitric Oxide Donor Sodium Nitroprusside on Stomatal Movement in Leaves of Mangrove Plant Aegiceras corniculatum

探索一种用材简单、操作方便、真实性强的观察红树植物桐花树叶片气孔的制片技术,并利用该技术研究不同浓度、不同处理时间的一氧化氮(nO)供体硝普钠(SOdIuM nITrOPruSSIdE,SnP)对桐花树气孔开闭的影响,探讨了nO调控的气孔运动与外源CA2+的关系以及nO与H2O2在调节气孔运动过程中的关系。结果表明:在搅碎法、指甲油印迹法、牛皮胶印迹法三种观察气孔方法中,牛皮胶印迹法是观察气孔开度变化的最佳方法。nO能够诱导桐花树气孔快速关闭,且表现出明显的时间效应与浓度效应。nO导致的气孔关闭与CA2+的参与有密切关系,nO与H2O2存在明显的协同效应,可以促进气孔关闭。The aim of the present paper was to find out a simple and easy-to-go method to observe the stomatal movement of a mangrove plant Aegiceras corniculatum.The new method was used to study the following areas, inculing the effect of exogenous nitric oxide (NO) on the leaf stomatal movement under different concentrations and different treated time, the effect of Ca2+ in the regulation of stomatal movement induced by exogenous NO, the interactional effect between NO and H2O2 in the regulationb of stomatal movement.The results suggested that the oxhide pastern print was the best way to observe stomatal movement of A.corniculatum compared with the blender method and the nail enamel print method.The stomatal closure induced by NO was associated with Ca2+ in dose dependent manner.It was also proved that a rapid stomatal closure could be co-induced by NO and H2O2 in A.corniculatum guard cells.国家自然科学基金项目(30770192;30670317);厦门大学“新世纪优秀人才支持计划”项目(X07115);湖北民族学院博士启动基金项

基于电子鼻的慢性胃炎气滞证患者常见病位的口腔呼气气味图谱研究

目的:运用电子鼻探讨慢性胃炎气滞证患者常见病位的口腔呼气气味图谱特征。方法:采用证素辨证的方法,筛选出397例慢性胃炎气滞证患者并判断病位证素,同时运用基于阵列式气体传感器技术的医用电子鼻(EN0l1103-A)采集其口腔呼气的气味图谱,选择气味图谱响应曲线的振幅、斜率作为图谱特征参数,借助分类器算法对慢性胃炎气滞证与非气滞证的口腔呼气气味图谱特征进行模式识别,比较慢性胃炎气滞证患者常见病位的口腔呼气气味图谱特征。结果:慢性胃炎气滞证的主要病位证素分布是胃(91.18%)、脾(38.29%)、肝(23.68%);主要病位为胃、脾胃、肝脾胃、肝胃;采用分类器算法对慢性胃炎气滞证的气味图谱进行模式识别时,运用随机森林算法,对气滞证的准确率可以达到65.85%;病位胃组、脾胃组的气味图谱响应曲线B、C、D、E、F、I、J的振幅均显著低于病位肝脾胃组(P<0.01);病位胃组、脾胃组的气味图谱响应曲线C、D、E、F、I的斜率均显著低于病位肝脾胃组(P<0.01),脾胃组曲线A的斜率低于病位肝脾胃组(P<0.05)。结论:运用电子鼻结合模式识别方法可初步判断慢性胃炎气滞证及其不同病位间的口腔呼气气味差异。国家自然科学基金项目(No.81373552);;福建省自然科学基金项目(No.2014J01362);;福建省中医药科研项目(No.wzpw201313);;福建省教育厅A类项目(No.JA14212);;载人航天领域预先研究项目(No.020104)~

Effects of Exogenous Nitric Oxide Donor SNP on Contents of Chlorophyll and Free Proline, Activity of Antioxidative Enzyme in Rice Seedlings under NaCl Stress

作者简介: 肖强(1970–), 男, 湖北恩施人, 博士, 研究方向为植物生理生化。Tel: 15971698508, E-mail: [email protected]通讯作者(Corresponding author): 郑海雷。E-mail: [email protected][中文文摘]在100mmolL-1NaCl胁迫下,研究了不同浓度一氧化氮供体硝普钠(sodium nitro prusside,SNP)处理对水稻叶片叶绿素、游离脯氨酸含量,叶片及幼根中愈创木酚过氧化酶(guaiacol peroxidase,GPX)、超氧化物歧化酶(super oxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性以及超氧阴离子产生速率等生理指标的影响。结果表明,适当低浓度SNP处理可以显著提高盐胁迫下水稻叶片中叶绿素和脯氨酸含量,并明显缓解盐胁迫下叶片和幼根受到的氧化性损伤;但在水稻幼苗不同器官,SNP调节的主要靶酶有所不同,在叶片中促进SOD和CAT活性,而在幼根中除SOD和CAT活性外,还提高GPX活性。[英文文摘] In this study, the contents of chlorophyll, free proline and the activities of GPX, SOD, CAT, and the producing rate of superoxide radicals in rice seedlings treated with a varying concentration of SNP under 100 mmol L-1 NaCl stress were investigated. The results showed that the contents of chlorophyll and free proline increased by treatment with low SNP concentration under salt stress. SNP alleviated significantly the oxidative damage caused by salt stress in leaf and root of rice seedlings. Different enzyme activities were regulated by SNP between leaf and root in rice seedlings under salt stress, SNP alleviated significantly the oxidative damage via promoting SOD and CAT activities in rice leaf, whereas, via regulating GPX activity mainly besides promoting SOD and CAT activities in root.国家自然科学基金项目(30770192,30670317,30271065);厦门大学“新世纪优秀人才支持计划”项目(X07115);湖北民族学院博士启动基金项

用流式细胞仪研究秋茄叶柄薄壁细胞大小及叶绿素含量与海水盐度的关系

采用快速离析方法和流式细胞仪研究了红树植物秋茄成熟叶柄薄壁细胞的大小和叶绿素含量与海水盐度之间的关系。结果表明 ,海水盐度为 0 .8%～ 2 .0 %时 ,随着盐度的升高 ,叶柄薄壁细胞大小和单位体积叶绿素含量的变化趋势与叶肉细胞一致。海水盐度为 0 .8%～ 1.0 %时 ,随着盐度升高 ,向有利于提高光合作用强度的方向发展 ;海水盐度在 1.0 %～ 2 .0 %之间时 ,随着盐度的升高 ,向加强生理干旱适应性方向发展。海水盐度高于2 .0 %时 ,叶柄薄壁细胞的大小和单位体积薄壁组织叶绿素含量随盐度的变化趋势与叶肉细胞不同 ,原因尚待进一步研究。实验结果表明 ,借助快速离析方法 ,使用流式细胞仪研究器官细胞相关特性是可行的 ,为从形态学角度研究红树植物耐盐机理提供了可能 ,扩大了流式细胞仪的应用领

Identification and analysis of odor of patients with exterior syndrome based on medical electronic nose

目的:运用自主研发的医用电子鼻探讨表证患者口腔呼气的气味图谱特征及气味识别准确率。方法:选择表证青年患者150例和健康青年人200名,运用基于薄膜型气体传感器阵列技术的医用电子鼻(nAnOfIlM-EnOSE MEdICAl001)采集口腔呼气的气味图谱,以响应曲线的振幅和上升斜率作为曲线特征,分析表证患者及其寒热病性的图谱特征,并进行判别分析。结果:表寒证和表热证患者的气味图谱响应曲线A、b、d、E、g、H的振幅和上升斜率均显著高于健康学生(P<0.01)。表热证患者的气味图谱响应曲线A、b、d、E、H的振幅和上升斜率均显著高于表寒证患者(P<0.05,P<0.01),表热证患者的气味图谱响应曲线g的振幅显著高于表寒证患者(P<0.01)。判别分析显示对健康青年人、表寒证青年患者、表热证青年患者口腔呼气的识别准确率分别为90.5%、86.5%、88.0%,对全部案例的识别准确率为89.1%。结论:表证青年患者口腔呼气的气味较健康青年人浓烈,且表热证青年患者气味变化较表寒证青年患者更明显,本医用电子鼻能够较为敏感和准确地辨识表证青年患者口腔呼气的气味特征及其寒热病性。医用电子鼻技术的发展可为中医嗅诊客观化研究提供手段和方法。Objective: to explore the odor response pattern characteristics of oral breath of patients with exterior syndrome and accuracy rates of odor identiication by medical electronic nose which was independent research and development.Methods: 150 young patients with exterior syndrome and 200 healthy young students were observed.Odor response patterns of oral breath were collected by the electronic nose(Nanoilm-Enose Medical001) based on a ilm of gas sensor array.The amplitude and rising slope of response curves were selected as curve characteristics to analyze pattern characteristics of exterior syndrome including exterior cold syndrome and exterior heat syndrome,and discriminant analysis was done.Results: In odor response patterns of patients with exterior cold syndrome and with exterior heat syndrome,the amplitudes and rising slopes of response curve A,B,D,E,G and H were signiicantly higher than those of healthy students(P<0.01).In odor response patterns of patients with exterior heat syndrome,the amplitudes and rising slopes of response curve A,B,D,E and H were signiicantly higher than those of patients with exterior cold syndrome(P<0.05,P<0.01),and the amplitude of response curve G was signiicantly higher than that of patients with exterior cold syndrome(P<0.01).Discriminant analysis showed that the accuracy rate of oral breath identiication of healthy students,patients with exterior cold syndrome and patients with exterior heat syndrome was 90.5%,86.5% and 88.0% respectively,and the accuracy rate of all cases was 89.1%.Conclusion: The odors of oral breath of patients with exterior syndrome were signiicantly stronger than those of healthy people.The odors of patients with exterior heat syndrome were signiicantly stronger than those of patients with exterior cold syndrome.This medical electronic nose could distinguish odor characteristic of oral breath of patients with exterior syndrome sensitively and accurately;and it could also distinguish odor characteristic of exterior cold syndrome from exterior heat syndrome.The development of medical electronic nose could provide a kind of new means for the study on the objectiication of TCM smelling examination.国家自然科学基金项目(No.30901899); 福建省教育厅A类项目(No.JA09132)~

Study on odor response patterns of patients with exterior syndrome based on electronic nose technology

目的:运用电子鼻技术探讨表证患者口腔呼气的气味图谱特征。方法:选择表证患者(青年学生)114例和健康青年学生255名,运用基于薄膜型气体传感器阵列技术的医用电子鼻(nAnOfIlM-EnOSE MEdICAl001)采集口腔呼气的气味图谱,以响应曲线的振幅和上升斜率作为曲线特征,分析表证患者及其寒热病性的图谱特征。结果:1表证患者的气味图谱响应曲线A、b、d、E、g、H的振幅和上升斜率均显著高于健康学生(P<0.05,P<0.01)。2表寒证和表热证患者的气味图谱响应曲线A、b、d、E、g、H的振幅均显著高于健康学生(P<0.05,P<0.01)。3表寒证患者的气味图谱响应曲线A、b、d、g的上升斜率显著高于健康学生(P<0.05,P<0.01)。表热证患者的气味图谱响应曲线A、b、d、E、g、H的上升斜率显著高于健康学生(P<0.05,P<0.01)。4表热证患者的气味图谱响应曲线A、b、d、E、H的振幅和上升斜率均显著高于表寒证患者(P<0.05)。结论:表证患者口腔呼气的气味较健康人浓烈,且表热证患者气味变化较表寒证患者更明显,本电子鼻能够较为敏感和准确地辨识表证患者口腔呼气的气味特征及其寒热病性,电子鼻技术的发展可为中医嗅诊客观化研究提供手段和方法。Objective: To explore the odor response pattern characteristics of oral breath of patients with exterior syndrome by electronic nose.Methods: 114 patients with exterior syndrome(young students) and 255 healthy young students were observed.Odor response patterns of oral breath were collected by the electronic nose(Nanofilm-Enose Medical001) based on a thin film of gas sensors array.The amplitude and rising slope of response curves were selected as curve characteristics to analyze pattern characteristics of exterior syndrome including exterior cold syndrome and exterior heat syndrome.Results: ①In odor response patterns of patients with exterior syndrome,the amplitudes and rising slopes of response curve A,B,D,E,G and H were significantly higher than those of healthy students(P<0.05,P<0.01).②In odor response patterns of patients with exterior cold syndrome and with exterior heat syndrome,the amplitudes of response curve A,B,D,E,G and H were significantly higher than those of healthy students(P<0.05,P<0.01).③In odor response patterns of patients with exterior cold syndrome,the rising slopes of response curve A,B,D and G were significantly higher than those of healthy students(P<0.05,P<0.01).In odor response patterns of patients with exterior heat syndrome,the rising slopes of response curve A,B,D,E,G and H were significantly higher than those of healthy students(P<0.05,P<0.01).④In odor response patterns of patients with exterior heat syndrome,the amplitudes and rising slopes of response curve A,B,D,E and H were significantly higher than those of patients with exterior cold syndrome(P<0.05).Conclusion: The odors of oral breath of patients with exterior syndrome were significantly stronger than those of healthy people.The odors of patients with exterior heat syndrome were significantly stronger than those of patients with exterior cold syndrome.This electronic nose could distinguish odor characteristics of oral breath of patients with exterior syndrome sensitively and accurately;and it could also distinguish odor characteristics of exterior cold syndrome from exterior heat syndrome.The development of electronic nose could provide a kind of new means for the study on the objectification of TCM smelling examination.国家自然科学基金项目(No.30901899); 福建省教育厅A类项目(No.JA09132)~

实时荧光PCR同时检测金黄色葡萄球菌和大肠杆菌O157:H7

目的建立改良分子信标-双重实时荧光PCR同时检测金黄色葡萄球菌和大肠杆菌O157:H7,应用于细菌性食物中毒的快速诊断。方法根据GeneBank公布的金黄色葡萄球菌nuc基因序列和大肠杆菌O157:H7rfbE基因序列,设计引物和改良分子信标探针,建立改良分子信标-双重实时PCR检测体系。结果双重荧光PCR反应体系检测151株金黄色葡萄球菌和27株大肠杆菌O157:H7,均出现特异的荧光信号,两种细菌检测互不干扰。对8762份大便、食品等标本进行检测,315份标本金黄色葡萄球菌实时荧光PCR阳性,其中286份金黄色葡萄球菌培养阳性;31份标本大肠杆菌O157:H7实时荧光PCR阳性,其中26份大肠杆菌O157:H7培养阳性。从样品处理到检测结果仅需要时间2h~1d。结论改良分子信标-多重实时荧光PCR检测体系快速、灵敏度高,特异性强,可用于金黄色葡萄球菌和大肠杆菌O157:H7食物中毒的快速诊断和肠道传染病的初筛,为食源性疾病的分子流行病学调查提供新的检测手段

半边旗二萜类化合物5F对人翼状胬肉成纤维细胞结缔组织生长因子表达和胶原合成的影响

[目的]观察半边旗二萜类化合物5F（5F）对体外培养的人翼状胬肉成纤维细胞（HPF）增殖和胶原合成及结缔组织生长因子（CTGF）表达的影响.[方法]利用手术切除的人翼状胬肉组织进行原代细胞培养,即得到HPF.将不同质量不同浓度（0、8、32、128）μg/mL的5F作用于HPF 24 h,通过噻唑蓝比色法（MTT）检测5F对细胞增殖的作用;用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹（Western blot）检测5F对细胞中CTGFmRNA及蛋白表达水平的影响;用化学比色法检测5F对细胞胶原蛋白含量的影响.[结果]在HPF中加入不同质量浓度（0、8、32、128）μg/mL的5F作用24 h,各组剂量的5F均可以明显抑制HPF的增殖（P＜0.01）,其半数抑制率（IC50）为32.12μg/mL.在8～128μg/mL的剂量范围内,5F能显著地下调CTGF mRNA（0.69±0.05,0.38±0.06,0.19±0.04,与对照组0.82±0.09比较,P＜0.05或P＜0.01）和CTGF蛋白（82.34±5.16,31.46±4.98,23.28±2.65,与对照组98.12±7.20比较,P＜0.05或P＜0.01）的表达水平,能显著地降低胶原蛋白的含量（13.95±1.49,12.53±0.51,5.89±0.80,与对照组16.19±1.02比较,P＜0.05或P＜0.01）,且均有明显的剂量-效应关系.[结论]半边旗二萜类化合物5F能显著地抑制体外培养的HPF的增殖和胶原蛋白的含量,使细胞的CTGF mRNA及蛋白表达明显下调,并且呈显著的量效关系,表明半边旗5F具有体外抗纤维化作用,这为寻找有效治疗HPF的药物提供了新的思路

Designable ultra-smooth ultra-thin solid-electrolyte interphases of three alkali metal anodes

该研究是在毛秉伟教授和董全峰教授共同指导下，由2014级博士生谷宇、2016级博士生王卫伟、2013级博士生李艺娟等同学通力合作的成果。泉州师范学院吴启辉教授和上海应用物理研究所樊春海研究员分别在X射线光电子能谱表征和同步辐射表征中提供了大力支持。化学化工学院郑明森副教授、颜佳伟教授、吴德印教授、iChEM研究员丁松园博士在实验和理论计算方面给予了大力帮助；田中群教授和郑南峰教授等对此工作提出了宝贵意见。 金属锂是下一代高能量密度二次电池最理想的负极材料之一。在以金属锂作为负极的电池中，锂电沉积过程中枝晶的生长及溶解过程的死锂现象是制约电池库仑效率、稳定性和安全性的重要因素，而金属锂表面的微观平整性及固态电解质界面（SEI）膜的组成和结构及由其所决定的电学和力学性质对锂电沉积和溶解行为有重要影响。 课题组所发展的电化学调控方法可进一步拓展到钠、钾碱金属负极上，形成大范围原子平整的钠、钾表面和超薄超光滑的SEI膜。特别是金属钠平面电极可在2 mA cm–2（1 mAh cm–2）100% Na DOD下稳定循环550周以上且库伦效率近100%。这一基于电化学调控的碱金属表面抛光和SEI膜构筑的方法及多尺度联合表征，为解决碱金属负极问题提供了新的思路，也为开展表面科学基础研究提供高质量的碱金属表面。【Abstract】Dendrite growth of alkali metal anodes limited their lifetime for charge/discharge cycling. Here, we report near-perfect anodes of lithium, sodium, and potassium metals achieved by electrochemical polishing, which removes microscopic defects and creates ultra-smooth ultra-thin solid-electrolyte interphase layers at metal surfaces for providing a homogeneous environment. Precise characterizations by AFM force probing with corroborative in-depth XPS profile analysis reveal that the ultra-smooth ultra-thin solid-electrolyte interphase can be designed to have alternating inorganic-rich and organic-rich/mixed multi-layered structure, which offers mechanical property of coupled rigidity and elasticity. The polished metal anodes exhibit significantly enhanced cycling stability, specifically the lithium anodes can cycle for over 200 times at a real current density of 2 mA cm-2 with 100% depth of discharge. Our work illustrates that an ultra-smooth ultra-thin solid-electrolyte interphase may be robust enough to suppress dendrite growth and thus serve as an initial layer for further improved protection of alkali metal anodes.This work was supported by the MOST projects (2015CB251102, 2012CB932902) and the NSFC projects (U1305246, 21621091, 21473147, 21533006, 21673193). 研究工作得到科技部973计划（项目批准号：2015CB251102、2012CB932902），国家自然科学基金（项目批准号：U1305246、21621091、21473147、21533006、21673193）等项目的资助