Development of aquantitative ELISA detection method for Varicella Zoster Virus(VZV) antigen

目的:建立水痘-带状疱疹病毒(VzV)抗原的双抗体夹心ElISA定量检测方法,用于质控VzV灭活疫苗研发和生产中抗原含量。方法:以VzV中和单抗5f6C8为包被抗体、8H5d1为酶标抗体,构建定量检测VzV抗原的双抗体夹心ElISA方法,并对本方法的特异性、灵敏度、准确性、线性和稳定性等性能进行分析。结果:建立的双抗体夹心定量检测VzV抗原的ElISA方法,线性范围为0.4μg~13μg/Ml,相关系数为r2=0.994,定量限度为0.4μg/Ml;变异系数CV80%。与VzV以外的相关病毒样本没有交叉反应。结论:构建的VzV抗原ElISA定量检测方法的各项性能符合定量检测需要,可用于VzV灭活疫苗的研发和生产过程的抗原含量检测。Objective:To develop a quantitative enzyme linked immunosorbent assay(Q-ELISA) to determine the concentration of Varicella Zoster Virus(VZV) antigen.This method was used to determine VZV antigen content at each stage of VZV inactived vaccine developing and manufacturing process.Methods: A double antibody sandwich Q-ELISA was developed to determine concentration of VZV antigen,which was based on the the high-affinity neutralizing monoclonal antibodies 5F6C8 as capture antibodies,and 8H5D1 as HRP-labeled antibody.The performance of reagent were evaluated.Results: The Q-ELISA for VZV antigen content was successfully developed.The reagent had good performance.The quantitation scope was 0.4 μg~13 μg/ml,The coefficient correlation was 0.994,the limit of detection was 0.4 μg /ml,the recovery was between 87.5% and 111.6%.The stability was up to 80% after reagent was heated for 6 days at 37℃.The variation coefficient was lower than 15%,and the reagent was no reaction with other sample except VZV antigen.Conclusion: The Q-ELISA for VZV antigen was developed with good specificity,accuracy and stability.The method can be used to determine VZV antigen content during development and production of VZV inactived vaccine

Originality and Vitality

陈耕(厦门市闽南文化研究会常务副会长):闽南语原创歌曲大奖赛分为三个阶段,5月份到7月10日,征集歌词322首入围100首,现在进入音乐创作阶段,将选拔出25首进入决赛,届时还将举办专门的演唱会。举办这次研讨会有两个主题,大陆的闽南语歌曲为何落后?如何提高我们的创作水平。我认为目前我们的闽南语原创歌曲有四个方面的问题:一是作曲重旋律,忽略了编曲(配器);二是样

Optimized Parameters for Electrochemical Fabrication of P-type Silicon-Based Two-dimensional Photonic Crystals

利用光刻技术与碱性腐蚀等工艺预写晶格图样,采用电化学腐蚀方法在P〈100〉型硅基底制备二维大孔硅光子禁带结构.结果表明:在预写有晶格图样的P〈100〉型硅基底上由电化学阳极氧化制备的二维大孔硅,其孔洞的生长速率、深宽比及表/侧面形貌与电解质配比方案及阳极电流密度均密切相关.在优化的电化学工艺参数下得到的空气洞阵列,具有近乎完美的二维四方晶格,晶格常数为3.8μm,孔洞直径约3.0μm,孔洞深宽约90μm,深宽比达30.该方法可用于制备在中红外或近红外波段具有完全二维光子带隙的光子晶体.A two-dimensional photonic band structure based on macroporous silicon with a gap centered at 20μm has been achieved for the first time.A medium doped p-type(100) silicon substrate was patterned by the standard photolithograpgy and alkaline efthing.And this pre-patterned sample was then etched 90 μm deep by electrochemical pore formation in the mixture of hydrofluoric-acid and dimethylformamide(DMF) to produce a quare lattice of circular air rods with a lattice constant of 3.8 μm and the very high aspect ratio up to 30.The optimized electrochemical parameters such as eletrolytical components and the current density were 5 % HF(by mass)+7 % H_(2)O(by mass)+DMF and 12 mA/cm~(2) respectively.the pore formation technique should allow the fabrication of photonic lattices with a complete two-dimensional photonic band gap in the middle and near infrared.作者联系地址：国防科技大学光子/声子晶体研究中心,国防科技大学光子/声子晶体研究中心,国防科技大学光子/声子晶体研究中心,国防科技大学光子/声子晶体研究中心,国防科技大学光子/声子晶体研究中心 湖南长沙410073,湖南长沙410073,湖南长沙410073,湖南长沙410073,湖南长沙410073Author's Address: Research Center of Photon and Phonon,National Univ.of Defense Technology,Changsha 410073,Chin

过滤、吸附及灭活病毒的纳米催化材料的快速筛选方法

本发明涉及催化材料，具体地说是一种过滤、吸附及灭活病毒的纳米催化材料的快速筛选方法，具体操作为：1)以100～1000个碱基的核酸分子片段作为探针，采用喷洒或浸泡的方式将核酸分子吸附于催化剂上，然后用不少于探针原液9倍的水进行洗脱，筛选出吸附能力强的、即吸附量为原液重量60％)以上的催化剂；2)将上述步骤选取的催化剂对目标病毒进行吸附、洗脱，对催化剂用水或生理盐水一次或多次洗脱，然后对洗脱液中的病毒进行传统的活性检测；筛选出对病毒的过滤、吸附及灭活作用好的、即吸附量为原液重量95％的催化剂。本发明首先以核酸分子作为探针，建立了对纳米催化材料吸附性能和抗水性能进行快速筛选和评价的方法。带填

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies