Empirical Research on Comprehensive Evaluation of Independent Innovation Capability of the Economic Zone of Taiwan Strait's Western Area

运用基于熵的模糊综合评价法和因子分析法,通过构建海峡西岸经济区自主创新能力评价指标体系,对该经济区自主创新能力进行综合评价。评价结果表明,海峡西岸经济区中厦门自主创新能力好,福州、温州、泉州、漳州自主创新能力一般,其他地区自主创新能力差,区域内部自主创新能力发展极不平衡。海峡西岸经济区要提高自主创新能力需进一步加大研发投入力度,强化自主创新的支撑和管理能力,提高自主创新产出效率。The paper evaluates the independent innovation capability of the economic zone of Taiwan Strait's western shore by establishing an evaluation index system and using fuzzy comprehensive evaluation method based on entropy weight and factor analysis method.The results show that the independent innovation capability of Xiamen is good.Fuzhou,Wenzhou,Quanzhou and Zhangzhou are in average level.The other areas are not good.The development of different areas' innovation capability in this economic zone is extremely unbalanced.The economic zone should further increase the momentum of R&D input,strengthen innovation support capacity and management capacity and improve innovation output efficiency.福建省软科学项目“海峡西岸经济区主导产业技术创新战略与路径研究”(2012R0086); 福建省社会科学规划课题“海峡西岸经济区自主创新与产业结构升级研究”(2011B228); 国家社会科学基金一般项目“全球生产网络背景下中国汽车产业升级研究”(12BJY073

Study on quality standard of Minghuanggao gel

目的:研究明黄膏质量标准。方法:采用薄层色谱(TLC)法对明黄膏中的主要成分大黄、黄连、苦参进行定性鉴别;高效液相色谱(HPLC)法测定明黄膏中大黄酚的含量。结果:TLC色谱中均能明显地检出大黄、黄连、苦参;HPLC法测得本品中大黄酚的含量为0.267~0.308 mg·g-1;在10.02~100.16 mg·L-1的范围内,溶液的浓度与峰面积呈良好线性关系,r=0.999 9;加样平均回收率为98.48% (n=6),RSD为1.20%。结论:本品定性、定量方法简便、准确,专属性强,质量标准能够控制该制剂的内在质量。OBJECTIVE To study the quality standard of Minghuanggao gel. METHODS Radix et Rhizoma Rhei, Rhizoma Coptidis and Radix Sophorae Flavescentis in Minghuanggao gel were identified by TLC. The content of chrysophanol in this preparation was determined by HPLC. RESULTS Radix and Rhizoma Rhei, Rhizoma Coptidis and Radix Sophorae Flavescentis in Minghuang-gao gel could be detected obviously by TLC. The content of chrysophanol in this gel was 0.267 - 0.308 mg·g -1 . The linear ranges were 10.02 - 100.16 mg·L -1 with good positive correlation. The average recovery of chrysophanol was 98.48% ( n =6),RSD= 1.20% . CONCLUSION TLC is a simple, method HPLC is accurate and reliable method. The quality standard can be used for quality control of this product.甘肃省科技厅中青年科技基金资助项目(编号:YS 011 A23 015

Distribution and polluting effects on Capitella capitata, Nephtys oligobranchia, Tharyx sp. on the intertidal mudflats in Shenzhen Estuary

在深圳河治理工程环境影响评估研究中 ,将 1 995年 7月至 1 998年 4月在 3个断面获得的 3种多毛类的数量进行分析 ,得出深圳河口泥滩 A断面和 RB断面的小头虫有明显的季节变化 ,即冬季数量较其他季节高 ,秋季数量较其他季节低 ,ET断面的小头虫数量没有明显的季节变化。RB断面寡鳃齿吻沙蚕数量随时间推移有增加的趋势 ,与有机质含量的变化是相似的。寡鳃齿吻沙蚕和独毛虫的水平分布明显受盐度限制 ,盐度较低的 ET断面寡鳃齿吻沙蚕数量较盐度较高的 A断面和 RB断面的低 ,独毛虫没有分布到 ET断面。 A断面的独毛虫在养猪场废水注入时大量繁殖。文中探讨了溶解氧和有机质含量与 3种小个体多毛类数量的关系。There are two famous Natural Reserve, Mai Po Marshes Reserve and Futian Natural Reserve, in Shenzhen (Deep) Bay. Deep Bay is an internationally important site for waterfowl and mangrove. Environmental stress in Mai Po and Fu Tian mudflats, induced by the accumulation of anthropogenic contaminants, such as heavy metals, poly-chlorinated biphenyl (PCB) and PAHs from industrial effluents, has aroused great concern after the Hong Kong Government declared Mai Po a wetland of international importance under the Ramsar Convention in September 1995.;High clay content, rich organic matter and high density of macrofauna are three ecological characteristics on intertidal mudflats in Shenzhen estuary. Capitella capitata, Nephtys oligobranchia and Tharyx sp. are commom species of small individual polychaete of the intertidal mudflats in Shenzhen Estuary. Capitella capitata is a worldwide species and it is considered as indicator of pollution. ;The data of three species of small individual polychaete got from July 1995 to April 1998 seasonly. Three transects including nine stations were designed for macrobenthic investigation. The study area was located in 114°00′18″E ～114°01′48″E, 22°29′51″N～22°31′66″N. Transect A includes stations A1, A2, A3 which are on the Shenzhen side of Shenzhen Bay. A3 is located near mangrove and the distances between A1 and A2, or between A2 and A3, were about 150 m. Transect RB and ET are on the Hong Kong side of the study area. Transect RB includes stations RBO, RB3 and 45O; Transect ET includes stations ET1, ET2 and ET4. Seasonal sampling was conducted in January (winter), April (spring), July (summer) and October (autumn). To collect benthic samples, a plastic core of 10 cm diameter and 20 cm length was inserted into the sediment to a depth 20cm. The sediment collected was transferred to a plastic bag and labeled with sample number. A total of five replicates were collected at each station. To wash the samples, they were transferred to a bucket and water was added. The slurry was then swung manually and then poured gently through a 0 5 mm sieve. The benthos and sand retained on the sieve were then transferred into bottle with formalin and Rose Bengal by forceps. The specimens were taken to laboratory for identification and counting under a microscope. After counting, three species of polychaete were dried in an oven at 60℃ for 48 hours. Regression analysis used the software SPSS 10.0. The seasonal variations of Capitella capitata, Nephtys oligobranchia and Tharyx sp. vary at three transects. Capitella capitata at transect A and transect RB had clearly seasonal variation. The quantities of Capitella capitata in winter were higher than that in other seasons in the same year. But there was no quantitative patterns for Capitella capitata at transect ET. There were increasing trends for the quantities of Nephtys oligobranchia at transect A and transect RB from July 1995 to April 1998, which was similar to the temporal patterns of organic matter. Regression analysis showed there was significant interrelation between the temporal patterns of organic matter and time. There was no any trend for the quantities of Nephtys oligobranchia at transect ET with the time going. The density and biomass of Tharyx sp. at transect A increased from July 1995 to April 1997, which may related to pig-cultural farms. There were many pig-culture farms near transect A before January 1997. The sewage of the pig-culture farms was discharged into the intertidal mudflat around transect A. There was no seasonal patterns for the density and biomass of Tharyx sp. at transect RB. Tharyx sp. was not found at transect ET during investigative period. ;The distributions of Capitella capitata, Nephtys oligobranchia and Tharyx sp. on the intertidal mudflats in Shenzhen Estuary were different in various transects. The average density and biomass of Capitella capitata (average value of three years) at transect A three years were lower than those at transect ET. But the average density and biomass of Nephtys oligobranchia at transec国家自然科学基金资助项目 (3 9670 1 3 5

壳聚糖絮凝法处理明黄膏提取液的研究

甘肃省自然科学基金暨中青年科技基金项目 (项目编号YS 0 11 A2 3 0 15

藏药镰形棘豆的化学成分、药理作用及毒理学研究进展

目的:为进一步研究和开发利用藏药镰形棘豆提供参考。方法:以"镰形棘豆""莪达夏""化学成分""药理作用""毒理学""Oxytropis""Oxytropis falcata""Oxytropis falcata Bunge""Chemical constituents""Chemical composition""Pharmacologic actions""Toxicology"等为关键词,组合查询2010年1月-2016年2月在Pub Med、中国知网、万方、维普等数据库中的相关文献,对镰形棘豆的化学成分、药理作用、毒理学研究及临床应用进行综述。结果与结论:共检索到相关文献81篇,其中有效文献46篇。目前从镰形棘豆中分离鉴定的化学成分主要包括黄酮类、生物碱类、甾体及萜类等化合物,其具有抗炎镇痛、抗脓毒症、抗氧化、防紫外线损伤、抗缺血缺氧性损伤、抗肿瘤、免疫调节、抗纤维化、促凝止血、抑菌、止咳祛痰等广泛的药理作用;毒性研究报道并不多见;镰形棘豆除在藏医中常被用来治疗疫疠、麻风、感冒、便血、骨伤、刀伤等之外,现已有一些以镰形棘豆为主要组成的上市药品和医院制剂应用于临床。今后应加强其药理、药效、作用机制和毒理学研究。原南京军区医学科技创新项目(No.14MS085

Polymorphism of Fatty Acid of Ralstonia solanacearum in Fujian Province

【目的】利用气相色谱技术检测福建省的40株青枯雷尔氏菌(Ralstonia solanacearum)菌株细胞内的脂肪酸,分析其脂肪酸分布的多态性;研究青枯雷尔氏菌脂肪酸多态性与青枯雷尔氏菌现有种下分化方法之间的关系。【方法】对40株青枯雷尔氏菌脂肪酸进行气相色谱分析,比较同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌脂肪酸的分布;对40株青枯雷尔氏菌脂肪酸进行聚类分析,分析聚成的各类青枯雷尔氏菌脂肪酸的特点以及脂肪酸多态性与其生理小种、生化型和致病性之间的关系。【结果】同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌,其脂肪酸都存在着明显的多态性;对40株青枯雷尔氏菌的脂肪酸进行聚类分析,可以聚成3类,即groupⅠ、groupⅡ和groupⅢ;青枯雷尔氏菌生理小种1存在着不同的脂肪酸类群,青枯雷尔氏菌脂肪酸多态性与其生化型之间不存在相关性,但是脂肪酸和致病性之间存在一定的相关性:groupⅠ为无致病性菌株,groupⅡ为过渡性菌株,groupⅢ为强致病性菌株。【结论】福建省青枯雷尔氏菌脂肪酸分布存在着明显的多态性;青枯雷尔氏菌脂肪酸多态性与致病性之间存在一定的相关性,脂肪酸有望成为青枯雷尔氏菌小种鉴定的新指标。【Objective】The fatty acids of 40 strains of Ralstonia solanacearum isolated from different hosts in the fields in Fujian Province were detected by gas chromatography (GC). The polymorphism of R. solanacearum fatty acids relating to the pathogenicity was observed. 【Method】 The MIDI system and cluster analysis were introduced in analyzing fatty acids to display the relations among the polymorphism, race, biovartype and pathogenicity. 【Result】 The results showed that the patterns of fatty acids were significant different in R. solanacearum strains both isolated from the different hosts and the different body parts of the same hosts. According to the fatty acids the strains were clustered into three groups, e.g. group Ⅰ relating to the strains with non-pathogenicity, group Ⅱ in which the strain pathogenicity was changeable with some virulent and avirulent ones, and group Ⅲ respondent to high pathogenicity. It was proved that the model of fatty acids has no relations to races and biovartypes in R. solanacearum. 【Conclusion】It is the fist time to describe the polymorphism of fatty acids in R. solanacearum in this paper. The pathogenicity could be grouped by the models of fatty acids to distinguish the pathogenicity, which could be used in the identification of R. solanacearum under species differentiation.国家“863”项目(2002AA244031);; 福建省青年科技人才创新项目(2003.J046

Application of biomarkers in marine environment monitoring

总结了国内外近十几年可应用于海洋环境污染监测常见的生物标记物及其主要的检测方法,对各自的应用特点进行了阐述,并展望了其应用的前景。其中对DNA损伤的检测作了较为详细的介绍。This paper sums up the recent-year application of biomarkers in marine environment motoring and the main detection methods of them.The features and the future prospects of these biomarkers are also discussed. The introduction of the DNA damage is the most detailed part in this paper.国家自然科学基金资助项目(A20077023,C40106012

Analysis of the nutrient distribution features and affecting factors in the Jiulongjiang estuary

根据2009、2010年“丰水期“和“枯水期“四航次九龙江河口混合区的调查资料,且结合历史资料对营养盐含量及分布特征、周日变化特征进行了统计和相关分析,研究了九龙江流域营养盐输入海洋的变化过程,探讨九龙江河口营养盐伴随潮汐变化,以及河口混合过程中的生物地球化学行为。调查期间溶解无机氮、硅和磷含量的平面分布呈现出由径流冲淡水高值向河口外海端递减的变化趋势;在涨潮时,河口区感潮段高溶解无机氮、硅、磷营养盐的陆源冲淡水与低溶解无机氮、硅、磷营养盐外海水相遇,随着外海水的侵入,外海水的作用逐渐加强,在稀释混合过程中呈现出无机营养盐逐步降低的变化趋势,退潮时则相反;营养盐在这复杂的河口过程中往往表现出在水动力的作用下稀释混合是主要过程,无机氮和活性硅酸盐在河口稀释混合过程中呈现保守性特征,活性磷酸盐在河口转移(补充)过程的行为复杂化,呈现缓冲作用为主。Based on the historical data and the data from four surveys in the wet season and dry season of 2009 and 2010 in the Jiulongjiang estuary,the concentration and distribution of nutrient were discussed in the mixing process.Furthermore,the daily variation of nutrient concentration with the tide was also discussed.The results showed that:(1) the concentration of total dissolved inorganic nitrogen,silicate and phosphate all decreased gradually from the river runoff to seawater;(2) in high tide,in the mixing process of the runoff with high nutrient concentration and the seawater with low concentration of nutrient,the concentration of nutrient decreased due to seawater.On the contrary,during the ebb,the concentration of nutrient increased due to the runoff;(3) in the mixing process in Jiulongjiang estuary,nitrogen and silicate were both conservatively diluted,but phosphate was buffered because of the complicated transfer and complement of phosphate.国家海洋局公益性项目(200805064);国家海洋局第三海洋研究所基本科研业务费项目(海三科2009021);海三科200900

Fingerprint analysis on methyl fatty acid and its applications in microbial study

脂肪酸甲酯谱图分析方法(Fatty Acid Methyl Ester,FAME)是鉴于脂肪酸可作为生物标记物而发展起来的分析技术。本文介绍了FAME谱图分析方法及其在微生物学领域的应用,包括在微生物检测、鉴定和微生物多样性研究中的应用。Fatty acid is an important biomarker.Its application in biology research has become increasingly popular.The method of FAME fingerprint analysis is based on fatty acid's utilization as a biomarker.This paper describes the FAME fingerprint analysis and its applications for microbial studies,including identification of unknown microbes and assessment of microbial diversity.国家863计划项目(2006AA10A211);; 福建省发展和改革委员会重点项目[闽农产(2006)10号];; 福建省青年科技人才创新项目(2003.J046