Functions and associated mechanisms of Human tumor necrosis factor-alpha-induced protein 8 family during gastric cancer carcinogenesis

胃癌是消化系统最常见的恶性肿瘤，在世界范围内，胃癌的发病率居恶性肿瘤的第四位，死亡率居恶性肿瘤的第二位。胃癌发生、发展涉及多因素、多步骤、多基因参与的复杂过程，迄今为止，胃癌的病因和发病机制尚未完全阐明。因此，对胃癌发病机制、在不同分子途径在肿瘤发生发展中的作用机制的研究，寻找特异性的生物标志分子，对于早期发现胃癌和探寻新的治疗方法，进而提高胃癌患者生存率和提升生活质量具有重要意义。 TNFAIP8家族是由肿瘤坏死因子α诱导产生的一个蛋白质家族，参与多种生物学过程，并在许多疾病特别是自身免疫性疾病和肿瘤的发生、发展中发挥重要的作用，TNFAIP8、TIPE2、TIPE3是其中的重要成员。TN...Gastric cancer(GC) is a leading cause of global cancer mortality responsible for 700000 deaths annually, and still highly prevalent in many parts of Asia, Eastern Europe and South America. Most patients with GC are diagnosed at advanced disease stages, and overall 5-year survival rates for patients with resectable GC remain low at 10-30% despite clinical advances in surgery and therapy.The elucida...学位：理学博士院系专业：医学院_生理学学号：2452013015431

Quantitative Detection of Genetically Modified Insecticidal Maize by ELISA

应用纯化的Bt1杀虫晶体蛋白质作为标准蛋白和免疫抗原 ,通过抗体 抗原 酶标抗体反应 ,建立了间接酶联免疫吸附测定法 (ELISA) ,以快速检测转基因抗虫玉米中的Bt1表达蛋白 .用建立的ELISA法对 4种进口玉米实物样品进行了测定 ,实验结果得到了免疫印迹分析的验证 ,并与进口试剂盒方法的定量分析结果相一致Based on the reaction among antibody antigen enzyme antibody, using purified Bt1 insecticide crystal protein as standard protein and immunity antigen, a quantitative indirect enzyme linked immunosorbent assay (ELISA) method was established to detect Bt1 protein expressed in genetically modified maize.4 import maize samples were detected by the ELISA, compared to western blotting & KIT assay methods. and results were agreeable.厦门市科技计划资助项目 (35 0 2Z2 0 0 110 9

Hardware Design for the Lifting Based Wavelet Transform in JPEG2000

离散小波变换是当今许多图像处理和压缩技术的基础 ,并被最新的ISO/IEC静态图像压缩标准JPEG2 0 0 0所采用 .基于提升方法的离散小波变换比传统的基于卷积的运算量小 .我们为JPEG2 0 0 0中的小波提升算法提出一个硬件结构 ,该结构整体运算速度高 ,存储需求低 ,硬件资源耗费少 .我们提出在数据通道之外实现边界扩展 ,以降低数据通道的复杂性 ,提高运算效率 .我们通过采用流水线技术 ,进一步提高了硬件设计的运算效率 .Discrete Wavelet Transform(DWT) has become very popular as the basis of many recent image processing and compression techniques and has been adopted by new image compression standard,i.e.,JPEG2000.The conventional convolution based DWT is computationally intensive.Such drawbacks can be overcome by using the lifting based scheme for the DWT.We presented a novel hardware structure for lifting based wavelet transform in JPEG2000.The hardware structure improves memory/speed tradeoff.Its computation speed is high and memory requirement is low.The complexity of datapath is reduced and the efficency of the system is improved by implementing the function of edge extension outside of data path.In the datapath design,we used pipeline technique to further improve the efficiency of the design.中国人民解放军总装备部预研项目 (No .41 31 60 60 1 ) ;; “十五”国防基础微电子预研项目 (No .41 30 80 1 0 4 0 8

智能网的多微机并行推理系统的研究

智能网络(intellegent network,IN)是计算机网络研究领域的一个新方向, 它要求计算机网络能够提供“ 操作 智能化”和“ 服务智能化” 。 A1型IN是由智能工作站(Intellgent workstation,IW)构成的智能网络 。 本文以中日国际合 作开发研究的A1型为背景, 重点讨论IW的多微机并行推理系统MMPIS的设计与实现航天基金资

Method Research of Improving Milling Precision of Industrial Robot

工业机器人普遍采用示教编程的方法,该方法利用操作者的观察获得精度,是制约工业机器人铣削精度的主要因素。为了提高工业机器人的铣削精度,提出了利用软件编程的方法,利用标定工具对工业机器人、零件夹具、铣刀夹具进行标定,求取它们之间的转换矩阵;利用标定时获得的转换矩阵,调整软件中的零件夹具、铣刀夹具的位置与姿态,使实际空间中的工业机器人、零件夹具和铣刀夹具的位置、姿态与软件空间中的工业机器人、零件夹具和铣刀夹具模型的位置、姿态关系一致;将软件空间编制的工业机器人铣削加工程序映射到实际机器人空间。由于建立的软件空间与实际空间具有一致性,所以工业机器人的标定精度即为铣削精度,实验结果表明:在标定后的软件空间编程,其铣削的位置精度可以达到0.2 MM,姿态精度可以达到0.1°,而示教编程一般的位置精度为0.5 MM,姿态精度为0.5°。At present,teaching programming is commonly used in industrial robot.The precision was obtained in this way through the watching of operator,which was the main reason that restricted industrial robot's milling precision.The software programming is proposed to use in order to improving the industrial robot's milling precision.First,calibration tool was used to calibrate industrial robot,parts fixture and milling cutter fixture to get the transition matrix among them.Then,the matrix was used to adjust parts fixture's and milling cutter fixture's position and posture in the software so that the industrial robot's,parts fixture's and milling cutter fixture's position and gesture in actual space were consistent with them in software space.At last,the industrial robot's milling procedure programmed in software space was mapped in actual space.Because the software space was consistent with the actual space,the industrial robot's calibration precision was the milling precision.The experimental results show that if the procedure is programmed in calibration software space,the positional accuracy can be 0.2 mm and the posture accuracy can be 0.1°.But if the procedure is programmed by teaching programming,the positional accuracy is common 0.5 mm,and the posture accuracy is common 0.5°

原子团簇P_(13)结构的密度泛函研究

使用分子建模软件设计出多种可能的P13团簇结构后,再用B3LYP密度泛函方法(6 31G 基组)进行几何优化和振动频率计算,得到了15种势能面上局域最小点结构(所有的振动频率均为正值),它们分别具有Cs和C2v对称性,原子团簇中的磷原子采用二或三配位成键.由楔状P8派生出的结构(Cs对称性)是最稳定的同分异体.从分子结构的几何形状上看,P8的楔状构型经常包含在P13的模型中,它是组成大分子磷原子的重要结构基元,可用来构造能量相对较低的大分子磷原子团簇

Development of Complex Probiotics

[目的]研制益生菌复合制剂。[方法]分离、筛选纳豆芽孢杆菌XW1、乳酸菌XW2、酵母菌XW3,进行动物灌胃试验与肠道菌群检测试验。[结果]通过饲喂益生菌复合制剂,小鼠肠道厌氧菌菌群有不同程度地增多,且一些致病肠杆菌受到抑制。综合分析,得到1个益生菌复合制剂的适宜配方(108 Cfu/M l):纳豆芽孢杆菌XW1:乳酸菌XW2:酵母菌XW3为2∶1∶1。[结论]益生菌复合制剂有促进动物生长,维持肠道微生态平衡的功效。[Objective] To develop complex probiotics.[Method] Probiotic strains including bacillus natto xw1,laciobacillus xw2,microzyme xw3 were isolated.Animal feeding experiment and intestinal flora detecting experiment were carried out.[Result] After feeding by complex probiotics,the counts of anaerobic bacteria in intestine of rats significantly increased,and the counts of some pathogenic E.coli significantly declined.Througy comprehensive analysis,a appropriate scheme of complex probiotics(108 cfu/ ml)was abtained∶bacillus natto xw1∶laciobacillus xw2∶microzyme xw3 was 2∶1∶1.[Conclusion]The growth of rats and balance of bacteria in rats intestine were promoted by complex probiotics

硫原子团簇负离子的螺旋结构

在使用B3LYP密度泛函进行几何构型优化和振动频率计算得到的硫原子团簇负离子的结构中,分子的总能量最低的S9- 到 S13-的同分异构体呈螺旋状构型。另外也计算了螺旋状的Sn- (n = 14~20)的结构。大多数的的硫负离子是链状结构,这与相应中性硫原子团簇的环状构型完全不同

Mechanism of retinoic acid receptor α-mediated growth inhibition of gastric cancer cells by all-trans retinoic acid

目的　探讨维甲酸α受体 (RARα)介导全反式维甲酸 (ATRA)抑制胃癌细胞生长的作用机理。方法　应用Northernblot分析维甲酸受体在胃癌细胞中的表达水平 ,通过脂质体转染方法将senseRARα和antisenseRARα分别转染到细胞中 ,通过MTT方法和软琼脂集落形成实验分析ATRA对转染细胞生长和恶性程度的影响 ,瞬时转染和测定氯霉素乙酰转移酶 (CAT)活性 ,分析维甲酸应答元件 (RARE)的转录活性。结果　ATRA诱导BGC 82 3细胞RARα表达 ,但不能诱导MKN 45细胞RARα表达。在转染细胞中 ,ATRA可以抑制转senseRARα的MKN细胞生长 ,但不能抑制转antisenseRARα的BGC细胞生长。与MKN 45细胞比较 ,ATRA诱导MKN/RARα细胞 βRARE的转录活性较高 ,但与BGC 82 3细胞比较 ,ATRA诱导BGC/aRARα细胞βRARE的转录活性则较低。 结论　足够量的RARα对于介导ATRA抑制胃癌细胞生长作用是必需的 【英文摘要】 Objective To study the mechanism of retinoic acid receptor α(RARα) in mediating growth inhibition of gastric cancer cells by all trans retinoic acid(ATRA).Methods Expression of RAR s was detected by Northern blot. After anti sense RARα or sense RARα had been transfected into gastric cancer cell lines BGC 823 and MKN 45 respectively, the inhibitory effect of ATRA on cell growth in stable clones was analyzed using MTT assay and colony forming assay in soft agar. The transcriptional activation of retin...国家自然科学基金资助项目(39880015)；国家杰出青年科学基金(B类)资助项目(39825502

The Organizational Control Strategy against Employee Counterproductive Work Behavior:An Empirical Research from Social Cognitive Perspective

本文基于社会认知的视角探讨中国企业员工反生产行为(CWb)的组织控制策略,采用跨层次分析的方法对27家企业的426名员工进行了实证研究。结果发现:虽然中国企业员工的尽责感和神经质人格特质对其CWb具有显著影响。但是,由于自利和规则导向的组织伦理气氛以及领导公正和信息公正作为重要的组织情境,不仅可以直接对CWb产生显著影响,而且还对人格特质与CWb之间的关系起到复杂的调节作用。因此,中国企业可以综合采用员工人格特质测试、组织伦理气氛培育以及组织公正环境建设等策略来进行员工CWb的组织控制。This article investigate the organizational control strategy of Chinese enterprises employee's counterproductive work behavior( CWB) from the perspective of social cognitive,using cross-level analysis method to do empirical research based on 426 employees from 26 enterprises.The results show that although Chinese employee's conscientiousness and neuroticism personality trait have the significant personality,but instrumentalism and rule oriented ethical climate,leader justice and information justice are very important organizational context,they not only have significant affect to CWB respectively,but also complex moderate the relationships between personality trait and CWB.So,Chinese enterprises can use a variety of strategies to control the employee's CWB such as employee personality test,organizational ethical climate cultivation,organizational justice environment construction,and so on.国家社会科学基金项目(14CGL018); 教育部人文社会科学规划基金项目(11YJA630057); 国家自然科学基金项目(71302175