中医头针结合综合康复疗法在小儿脑性瘫痪中的应用

目的探讨中医头针结合综合康复疗法在小儿脑性瘫痪中的应用效果。方法研究对象为某院收治的脑瘫患儿97例,收集时间为2015年1月至2016年6月。按照随机数字表法分为观察组和对照组。对照组给予头针进行治疗,观察组在对照组头针治疗基础上,结合EMGBF治疗仪、按摩、作业训练等综合康复疗法。观察两组治疗效果。结果观察组总有效率93.9%明显优于对照组81.3%,组间比较,差异有统计学意义,P<0.05;两组粗大运动功能测试量表(GMFM-66)、痉挛评定量表(Ashworth)评分治疗后均明显改善,这说明两组粗大运动功能和肌张力明显改善,且观察组治疗后GMFM-66、Ashworth评分均明显优于对照组,组间比较,P<0.05;观察组生活活动能力量表(ADL)评分结果示,观察组日常生活能力、精神状态、社会活动能力因子评分均明显高于对照组,组间比较,P<0.05。结论中医头针结合综合康复疗法治疗小儿脑性瘫痪能够明显提高整体治疗效果,改善患儿的运动功能、肌张力以及生活活动能力

Characterization and application of nucleocapsid protein of porcine reproductive and respiratory syndrome virus expressed in E.coli

从已构建的PRRSV ORF7重组质粒pUCm-T-ORF7中用PCR扩增ORF7基因亚克隆至表达载体pGEX-4T-3,构建重组表达质粒pGEX-4T-3-ORF7并转化大肠杆菌。经SDS-PAGE及Western blotting鉴定,成功表达了谷胱苷肽转移酶(GST)融合的核衣壳蛋白(N),重组N蛋白表达量约为菌体总蛋白的35%,主要以可溶的形式存在,且能形成同源二聚体。重组N蛋白经谷胱苷肽凝胶(glutathione sepharose 4B)亲和层析后得到高度纯化,并将该蛋白作为抗原建立了间接ELISA检测方法。利用该方法对某猪场76份猪血清进行检测并将结果与IDEXX公司ELISA试剂盒检测结果作比较,2种方法的总符合率达93.4%,检测结果之间差异不显著(P>0.05)。结果表明大肠杆菌表达的重组GST融合N蛋白具有良好的抗原性,因而有望利用该重组蛋白开发为试剂盒应用于临床PRRSV抗体的检测。Porcine reproductive and respiratory syndrome virus(PRRSV) FJ-1 was a newly identified virus isolate in Fujian province.The ORF7 gene of FJ-1 was amplified by RT-PCR and cloned into vector pUCm-T,then subcloned into expression vector of pGEX-4T-3.The recombinant GST-tagged nucleocapsid protein(rN) was expressed in E.coli and the molecular weight was approximately 38 000 as identified by SDS-PAGE and Western blotting.Expression level of the rN protein was approximately 35% of the total bacterial protein and mostly soluble.The rN protein was purified to homogeneity using GST affinity chromatography.Analysis of the rN protein under nonreducing conditions revealed that similar to native protein,the rN protein also possesses homo-dimerization property.An indirect enzyme-linked immunosorbent assay(ELISA) for detecting PRRSV antibody was developed using the purified rN protein as antigen.76 serum samples were detected by the method and the result was compared with that using IDEXX PRRS HerdChek ELISA kit.An identity of 93.4% was revealed between the two ELISA kits and no significant difference(P>0.05) was detected.The data indicate the rN protein has the potential usefulness for detection of the PRRSV antibodies.福建省科技攻关计划重点资助项目(2003N083);; 厦门市科技攻关计划重点资助项目(3502Z20031054

In vitro study of cholesterol succinyl chitosan anchored liposomes as a carrier for epirubicin

背景:多糖"锚定"脂质体在抗肿瘤药物、蛋白以及基因的传输领域有着极其重要的理论及应用价值,国外已有较多机构在进行相关的研究。目的:通过"锚定"的方式制备胆甾醇琥珀酰基壳聚糖锚定脂质体,并以表阿霉素作为模型药物,考察其对包载药物体外释放性质的影响。设计、时间及地点:体外实验,于2006-09/2008-05在天津市生物医学材料重点实验室完成。材料:以壳聚糖为原料,合成胆甾醇琥珀酰基壳聚糖,并采用胶体滴定法测定其胆甾醇基取代度。方法:采用pH梯度法制备表阿霉素脂质体,然后通过共孵育的方法合成了取代度为2.80%,5.58%和8.00%的载药胆甾醇琥珀酰基壳聚糖锚定脂质体。主要观察指标:荧光分光光度计检测药物浓度;透射电镜观察脂质体形态;亚微米粒度及电位分析仪检测脂质体的粒径大小、分布和电位;动态透析法考察包载药物表阿霉素在胆甾醇琥珀酰基壳聚糖锚定脂质体中的体外释放特征。结果:胆甾醇琥珀酰基壳聚糖锚定脂质体为规则球状形态,呈现典型的核壳结构,粒径为245.4~279.7nm,zeta电位为+21.09~+25.48mV;和载药脂质体及壳聚糖包衣脂质体相比,CHCS锚定脂质体能明显延缓表阿霉素的体外释放,在胆甾醇基取... 【英文摘要】 BACKGROUND: Polysaccharides anchored liposomes play an extremely important role in the fields of antitumor drug, protein and gene transmission. Related research is also present abroad. OBJECTIVE: To prepare cholesterol succinyl chitosan (CHCS) anchored liposomes, and to investigate the effect of CHCS anchored liposomes on the release property in vitro of loading drugs taking epirubicin as a model drug. DESIGN, TIME AND SETTING: A study in vitro was performed in the Key Laboratory of Biomedical Materials o

软件定义FiWi网络中网络编码控制方法

针对传统FiWi（光纤无线混合接入）网络中网络编码控制过程中大量Gate、Report信息交换导致的网络信息复杂、编码时延增大等问题,设计了一种支持编码功能的软件定义FiWi网络架构和基于Openflow协议的网络编码控制方法。仿真结果表明,所提出的软件定义FiWi网络架构中的集中编码控制方法及其实现方案能够减少网络中控制信息数量,提高带宽利用率,有效降低数据传输时延并提高网络吞吐量

黑龙江扎龙国家级自然保护区鼓藻类植物中国新记录

于2011年5月(春季)、7月(夏季)和10月(秋季),先后3次对黑龙江扎龙国家级自然保护区进行鼓藻类植物调查,并对采集到的57份标本进行鉴定。结果发现,有6个分类单位为鼓藻类植物中国新记录,隶属于5个属,分别为:微小胶球鼓藻(Cosmocladium pusillum Hilse),中凹鼓藻(Cosmarium medioretusum Coesel),近前膨胀鼓藻锥形变种(Cosmarium subprotumidum var.pyramidale Coesel),双孢辐射鼓藻美国变种Actinotaenium diplosporum var.americanum(West et West)Teilin

黑龙江扎龙国家级自然保护区鼓藻类植物中国新记录

于2011年5月(春季)、7月(夏季)和10月(秋季),先后3次对黑龙江扎龙国家级自然保护区进行鼓藻类植物调查,并对采集到的57份标本进行鉴定。结果发现,有6个分类单位为鼓藻类植物中国新记录,隶属于5个属,分别为:微小胶球鼓藻(Cosmocladium pusillum Hilse),中凹鼓藻(Cosmarium medioretusum Coesel),近前膨胀鼓藻锥形变种(Cosmarium subprotumidum var.pyramidale Coesel),双孢辐射鼓藻美国变种[Actinotaenium diplosporum var.americanum(West et West)Teiling],双臂角星鼓藻优美变种[Staurastrum bibrachiatum var.elegans(West et West)Prescott],冠毛多棘鼓藻钩状变种波兰变型(Xanthidium cristatumvar.uncinatumf.polonicumGutwingski)

Amplification and Sequence Analysis of ORF7 Gene of Porcine Reproductive and Respiratory Syndrome Virus

根据猪繁殖和呼吸综合征病毒(PRRSV)已发表的核苷酸序列,设计了1对特异性引物P1/P2,用RT-PCR扩增了PRRSV福建毒株FJ-1和FJ-2的全长核衣壳蛋白基因(ORF7),将扩增产物连接到pUCm-T载体并转化大肠杆菌DH10B,提取阳性重组质粒进行序列测定与分析.结果表明:FJ-1和FJ-2毒株ORF7基因均为372 bp,编码123个氨基酸,与美洲型参考毒株VR-2332及欧洲型参考毒株LV的核苷酸同源性分别为95.16%,94.35%和61.40%,60.65%,其推导的氨基酸同源性分别为95.93%,95.12%和56.49%,55.73%.研究表明,福建流行的FJ-1和FJ-2属于美洲型PRRSV毒株.Porcine Reproductive and Respiratory Syndrome Virus(PRRSV) strains FJ-1 and FJ-2 were isolated from a pig farm in Fujian province of China during an outbreak of PRRS.The full-length of ORF7 gene encoding the nucleocapsid protein(N) protein of FJ-1 and FJ-2 was amplified by RT-PCR with a pair of primers(P1/P2) and cloned into pUCm-T vector,and transformed to E.coli DH10B competent cell.The purified recombinants were subjected to sequencing and analysis.The ORF7 genes of FJ-1 and FJ-2 were both 372 bp and encoding 123 amino acids.They displayed 95.16 %,94.35 % and 61.40 %,60.65 % nucleotide identities to strains VR-2332(a reference strain of America) and LV(European genotype),respectively.The deduced amino acids of FJ-1 and FJ-2 showed 95.93 %,95.12 % and 56.49 %,55.73 % identities to strains VR-2332 and LV.The results shown here strongly suggested that PRRSV strains FJ-1 and FJ-2 belong to American genotype.福建省科技攻关计划重点项目(2003N083);; 厦门市科技攻关计划重点项目(3502Z20031054

Prokaryotic Expression and Diagnostic Application of ORF2 of Porcine Circovirus Type Ⅱ

II型猪圆环病毒(PCV2)是引起断奶仔猪多系统衰竭综合症(PMW S)的重要病原.本研究用PCR以构建的PCV2ORF2重组质粒pBS-T-ORF2为模板,扩增截短的ORF2(tORF2)基因亚克隆至表达载体pGEX-4T-3,构建重组表达质粒pGEX-tORF2并转化大肠杆菌BL21(DE3).经SDS-PAGE及W estern b lot鉴定,成功表达了谷胱甘肽S转移酶(GST)融合的tORF2,重组蛋白分子量约为45ku,表达量约为菌体总蛋白的20%,重组蛋白具有良好的免疫学活性.用tORF2重组蛋白对20份临床猪繁殖与呼吸障碍综合征(PRRS)阳性猪血清进行W estern b lot检测,有4份(20%)血清显示PCV2抗体阳性,说明PCV2与PRRSV存在共同感染现象.本研究初步证明表达的tORF2重组融合蛋白可以应用于临床检测.Porcine circovirus type Ⅱ(PCV2) plays a crucial role in the pathogenesis of post-weaning multisystemic wasting syndrome(PMWS) in swine.The ORF2 gene encoding the capsid protein of PCV2 was amplified by PCR and cloned into plasmid pBS-T to construct recombinant plasmid pBS-T-ORF2,which was used as template to amplify the truncated ORF2(tORF2).The tORF2 gene was consequently cloned into the expression vector of pGEX-4T-3 for prokaryotic expression in E.coli.Expression level of the recombinant GST-tagged tORF2 protein reached approximately 20% of the total bacterial proteins and its molecular weight was approximately 45 ku identified by SDS-PAGE and Western blot.The recombinant protein was used as antigen to establish a Western blot diagnostic assay.Twenty PRRS positive swine serum samples were detected by this Western blot assay.The positive rate of these sera was 20%(4/20),indicating that coinfection of PRRSV and PCV2 exist in swine.Taken together,the recombinant tORF2 protein reporteded in the present study may play a critical role in the detection of PCV2 antibodies immunologically.福建省科技攻关计划重点项目(2003N083);; 厦门市科技攻关计划重点项目(3502Z20031054)资