A Rapid Screening Assay for Insecticidal Antibiotic Produced by Marine Actinomycetes

报道了以卤虫为指示动物，采用双层琼脂扩散方法构建的一种杀虫微生物筛选模型。该模型具有灵敏度高，快速简便等优点，适合于海洋微生物杀虫活性物质的早期大量初筛工作。利用该模型对１２４０株分离于厦门海区潮间带的放线菌进行了初筛，杀虫阳性率为２．３％，展示了海洋微生物是杀虫活性物质研究和开发的重要来源。A rapid screening assay for insecticidal microorganisms is described in this paper. The double-layer agar and brine shrimp (Artemia salina ) used as bioassay, are involved. The assay is highly sensitive, and easy to operate, and is suitable for the early screening for insecticidal activity materials from marine microorganisms. 1240 cultures, isolated in the tidal zone of the Xiamen sea area, have been examined, and 2.3% is given as producing inseticidal activity materials. The present work indicates that marine microorganisms could be an important source of inseticidal activity substances

Screening of marine bacterial strain with immunoactive exopolysaccharide

从厦门海区潮间带的动植物体及底泥分离到的177株海洋细菌中筛选出一株高产胞外多糖的1202菌株.其产生的胞外多糖可显著地促进昆明种小鼠脾淋巴细胞IL 2(白介素 2)的合成,并对小鼠S180肉瘤具有较强的抑制作用.经初步分类鉴定,确定该菌株归属于土壤杆菌属(Agrobacteriumsp.).An exopolysaccharide-producing strain 1202 was obtained by screening 117 marinebacteria isolates, which were from the flora ,the fauna and the sediments in the intertidal zone of the Xiamen coast. The exopolysaccharides produced by the bacterial strain 1202 could stimulate interleukin 2 (IL-2) secretion from mouse spleen lymphocytes in vitro, and exhibit significant inhibitory effects on sarcoma 180 solid tumors in vivo. The strain 1202 was tentatively identifiedas a species of the genus Agrobacterium, based on its morphology, growth features, physiological and biochemical characteristics.国家自然科学基金资助项目(30070024

Suitable Culture Conditions for Exopolysaccharides Produced by Marine Bacterial Strain 1202

应用响应面法(ResponseSurfaceMethod,RSM)对海洋细菌1202菌株胞外多糖发酵培养基进行优化.结果表明,适宜的发酵培养基组成为:蔗糖4%,酵母膏0.05%,玉米浆1.5%,CaCO30.15%,KH2PO40.08%,MgSO4·7H2O0.05%,FeSO4·7H2O0.01%,NaCl0.1%,pH7.5.在装液量为50mL(500mL三角瓶),接种量5%时,于28℃摇瓶培养7d,胞外多糖的产量可达到8.3mg/mL,转化率为20.75%.The response surface method (RSM) was used to optimize the medium for production of exopolysaccharides by marine bacterium 1202. The optimal fermentation conditions were established with 5% inoculum size and 500 mL Erlenmeyer flask containing 50 mL medium, which consisted of 4% sucrose,1.5% corn steep liquor,0.05% yeast extract,0.15% CaCO3,0.05% MgSO4·7H2O,0.08% KH2PO4,0.01% FeSO4·7H2O,0.1% NaCl,pH7.5.The fermentation was carried out at 28℃ for 7 days on a rotary shaker.Under these conditions, the yield of exopolysaccharide reached 8.3 g/L,and the conversion rate of sucrose was 20.75%.国家"863"计划(2001AA620401);; 国家自然2100.651.30.25科学基金(30070024)资

极化电位对铜系防污涂层性能的影响

采用电化学阻抗谱研究了外加0.0、-0.7和-0.9 V(相对于饱和甘汞电极)极化电位对铜系防污涂层在青岛海域海水中防护性能的影响,采用三维电子显微镜对涂层腐蚀形貌进行观察,以原子吸收分光光度计测量介质中铜元素的含量来表征防污剂(氧化亚铜)的溶解速率,并进行了6个月的实海试验。结果表明,极化电位加速了涂层的阴极剥离过程,明显降低了其防腐和防污性能