A Rapid Screening Assay for Insecticidal Antibiotic Produced by Marine Actinomycetes

Abstract

报道了以卤虫为指示动物,采用双层琼脂扩散方法构建的一种杀虫微生物筛选模型。该模型具有灵敏度高,快速简便等优点,适合于海洋微生物杀虫活性物质的早期大量初筛工作。利用该模型对1240株分离于厦门海区潮间带的放线菌进行了初筛,杀虫阳性率为2.3%,展示了海洋微生物是杀虫活性物质研究和开发的重要来源。A rapid screening assay for insecticidal microorganisms is described in this paper. The double-layer agar and brine shrimp (Artemia salina ) used as bioassay, are involved. The assay is highly sensitive, and easy to operate, and is suitable for the early screening for insecticidal activity materials from marine microorganisms. 1240 cultures, isolated in the tidal zone of the Xiamen sea area, have been examined, and 2.3% is given as producing inseticidal activity materials. The present work indicates that marine microorganisms could be an important source of inseticidal activity substances

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