Evolutionary and Transcriptional Exploration of Karyopherin β superfamily proteins

β核转运蛋白家族是真核细胞中广泛存在的一类蛋白质，在生物大分子核质转运这一细胞过程中扮演着关键角色。由于β核转运蛋白家族各成员间序列组成及结构上的差异，其所携带的底物及工作机制也有所不同。目前国际上对该家族的研究尚浅，对其家族的形成及功能演化也尚未明晰。在本研究中，我们利用系统发育分析，表达谱芯片分析，转录因子调控位点预测等方法，对酿酒酵母，秀丽隐杆线虫，黑腹果蝇，斑马鱼，爪蟾，原鸡，小鼠和人类基因组中β核转运蛋白家族成员开展了进化、表达和基因调控模式分析。在酵母的14个家族成员中，8个成员（KAP95,KAP114,KAP120,KAP123,CSE1,MSN5,CRM1和LOS1）直接与它...Karyopherin β superfamily is a group of proteins which widely spread in eukaryotic cells. They play a key role in mediating nucleocytoplasmic transport of macromolecules. Because of their diversity in sequence compositions and structure, they bind varied cargos and function in different ways. Currently karyopherin β superfamily is not well studied worldwide, and it’s still unclear about the origin...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：2172006115224

Analysis and estimation of learning style in Library

根据南京师范大学社科专业图书馆2016年4.22—5.22的在馆读者信息,以可反映学习状态的参数——在位人数、总占位数及吃零食、玩手机、用电脑、睡觉、交谈、学习等的人数,对该馆内读者的实时学习状态进行了抽样分析。首先,通过读者出入馆信息确定了状态参数的抽样时间为上午10—11时,并定义学风指标为学习人数除以实际在位人数;再对抽样时间点的学习状态参数及天气情况建立模型。通过建立负二项回归模型、广义线性模型,并应用稳健性检验方法得到了在馆读者的实时学习状态。结果表明：学风指标区间为60%-80%,处于良好状态;8—11时的进馆人数与学习氛围呈正相关关系,天气因素对馆内读书人数有较大影响,其是无法改变的外生变量,可通过改善图书馆的硬件设施与服务质量提升进馆人数;模型估计的学风指标与实际情况基本一致。Based on the present readers＇ information from Nanjing Normal University professional library between Apr 22 th and May 22 th in 2016, the readers＇ real-time learning style are analyzed by some parameters reflecting learning status such as the present number, the total number of occupied seats, and the numbers of who are eating snack, playing phones, using computer, sleeping, talking and learning. Firstly, the readers＇ entrance information are used to ensure that the sampling time of status parameters is 10—11 a.m. each day, and we define the learning style index as the number of who are learning divided by the real number of who are present seated. Secondly, a model is put forward based on the learning status parameters and weather conditions from sampling time point. By the Negative Binomial Regression, Generalized Linear Model and robust test, the real-time learning status of present readers is put forward. The results show that the interval of the learning style is 60%-80%, which is good; there is a positive correlation between the entrance number of 8—11 o＇clock and the learning atmosphere. The weather condition is an unchanged exogenous variable that affects the number of readers. The improved library＇s hardware facilities and service quality can increase the entrance number. The index made by the model is basically consistence with the actual circumstance.国家自然科学基金（61374080）; 江苏省自然科学基金（BR20161552

基于枯草芽孢杆菌重组菌株构建技术制备海洋源金属硫蛋白

为构建安全高效表达海洋源金属硫蛋白的益生菌重组菌株,以海洋源褶牡蛎内脏团为模板,PCR克隆得到褶牡蛎金属硫蛋白基因(Op-MT),大小为324 bp,经pMD19-T载体亚克隆到融合表达载体pHT43-SUMO中,然后通过电转化法将重组质粒转入八种蛋白酶缺陷的宿主枯草芽孢杆菌(Bacillus subtilis)WB800N中。SDS-PAGE电泳显示诱导表达的融合蛋白SUMO-Op-MT分子量约为23 kD,与预期一致,说明可溶性融合蛋白SUMO-Op-MT在宿主菌中得到了表达,且通过Western blotting进一步得到验证。同时,HPLC检测重组枯草芽孢杆菌发酵液,结果也表明重组枯草芽孢杆菌成功表达了可溶性目的蛋白Op-MT。通过对重组工程菌中MT活性检测发现,其对重金属镉耐受力增强,说明重组工程菌成功表达出有活性的金属硫蛋白。这为以后开发天然、高效海洋源重金属解毒剂提供理论基础。福建省科技计划项目(2017N0016);;国家海洋局第三海洋研究所基本科研业务费专项资金资助项目(海三科2016031)共同资

分泌AFP的胃肝样腺癌(光镜、电镜和免疫组化观察)

分泌AfP的胃肝样腺癌（光镜、电镜和免疫组化观察）江显毅1杨善民2张思宇1方庆全1关键词胃癌；肝癌细胞样分化；电镜；免疫组化1.厦门市第一医院（厦门361003）2.厦门大学抗癌研究中心自1982年PrATH［1］等首次在卵巢的卵黄囊肿瘤发现肝癌细胞..

Effects of NaCl Stress on Photosynthesis of Three Bamboos

采用盆栽试验法,对花叶唐竹(SInObAMbuSA TOOTSIk f.luTEOlO-AlbO-STrIATA)、小琴丝竹(bAMbuSA MulTIPlEX‘AlPHOnSE-kArr’)和刺黑竹(CHIMOnObAMbuSA nEOPurPurEA)进行不同浓度盐(nACl)胁迫(Ck、0.1%、0.3%、0.5%、0.7%、1.0%)处理,用美国lI-COr公司生产的lI-6400便携式光合作用分析系统测定其各项光合作用相关指标,分析研究其中重要的4项指标:净光合速率(Pn)、气孔导度(gS)、胞间二氧化碳浓度(CI)和蒸腾速率(E),以探究nACl胁迫对3种竹子叶片光合作用的影响。结果表明:低盐胁迫(0.1%nACl处理)提高了小琴丝竹和刺黑竹叶片净光合速率、气孔导度和蒸腾速率,而对花叶唐竹影响不大,但随着盐胁迫浓度的增加,3种竹类植物叶片的净光合速率、气孔导度和蒸腾速率呈逐渐下降的趋势;3种竹类植物胞间二氧化碳浓度随盐胁迫浓度的提高变化规律不明显。Four important indices of photosynthesis,including net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(E) and intercellular CO2(Ci) of the three bamboos of Sinobambusa tootsik f.luteolo-albostriata,Bambusa multiplex ‘Alphonse-karr’ and Chimonobambusa neopurpurea were studied by LI-6400 Portable Photosynthesis System and treatments by different concentrations of NaCl(CK-0.0%,S1-0.1%,S2-0.3%,S3-0.5%,S4-0.7% and S5-1.0%) in order to discuss the effects of NaCl stress on the photosynthesis of bamboos.The results showed that: low concentrations of NaCl stress(0.1%) promoted the Pn,Gs and E of Bambusa multiplex ‘Alphonse-karr’ and Chimonobambusa neopurpurea,but little effect on Sinobambusa tootsik f.luteolo-albo-striata;from 0.3% NaCl stress to 1.0% NaCl stress,with the increasing of salinity,Pn,Gs and E of all three bamboos were decreased;Ci of all three bamboos was not in good relationship with the increasing of salinity.厦门市科学技术局科研项目(No.3502Z20102003); 厦门市市政园林局科研项目(No.YK-2000-02

Electrogenic and Anti-Methanogenic Properties of Bacillus cereus for Enhanced Power Generation in Anaerobic Sludge Driven Microbial Fuel Cell

Mutual interactions between microorganisms play a vital role in the formation of electroactive biofilms, which is a key element in the longevity and success of bioelectrochemical systems. The present study was intended to examine both the electrogenic properties of B. cereus and its ability to inhibit methanogenesis in microbial fuel cells (MFCs). The potential influence of the incorporation of B. cereus into anaerobic sludge (AS) on the electrochemical activity was assessed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) analyses. The CV of MFCs with B. cereus showed a strong redox peak, suggesting that B. cereus has electrogenetic properties. Moreover, the incorporation of B. cereus into AS provided an enhancement in the power generation (4.83 W/m3) and the CE (22%) of the MFC compared to the corresponding values for an MFC inoculated solely with AS (1.82 W/m3, 12%). The increase in power generation could be due to the antimethanogenic property of B. cereus, which was evident from the 54% reduction in methane production. The results of this study suggest that the incorporation of microorganisms with electrogenic and antimethanogenic properties into AS promotes the formation of electroactive biofilms and maximizes the power generation of MFCs by suppressing the methanogenesis

多种手势对应同一语义的柔性映射交互算法的研究

针对智能交互界面中手势识别错误导致交互界面变化错误和手势不识别两个基本问题,本文设计并实现了基于手势交互的智能教学界面,该系统可以通过获取教师的手势信息与教师进行交互.主要创新点在于提出了多种手势对应同一语义的柔性映射交互算法.本文选取了14种自然交互手势,分析了对应同一语义的多种手势之间的共同特征.实验结果显示,该算法能够有效降低用户负荷.该算法已经用于一个基于手势交互的智能教学系统界面中.国家重点研发计划(No.2018YFB1004901)国家自然科学基金(No.61472163,No.61603151)山东省重点研发计划(No.2017GGX10146

江西南昌西汉海昏侯刘贺墓出土玉器

江西南昌西汉海昏侯刘贺墓（M1）出土玉器[1]约400件（套）,包括约40种器形。全部玉器分散存放在墓室的不同区域（墓室平面图参见本期第5页图一）,表现为不同区域器形有别,具有不同的功能属性。据此特点,本文按出土玉器的不同区域划分,共甄选出具有代表性的38件玉器介绍如下。一西藏椁（一）娱乐用器库共出土玉器13件,其中舞人玉佩、双龙首国家社科基金重大委托项目“海昏侯墓考古发掘与历史文化资料整理研究”(项目编号:16@ZH022)子课题“海昏侯墓出土文物研究”成果之

Controlling Reversible Expansion of Li2O2 Formation and Decomposition by Modifying Electrolyte in Li-O2 Batteries

锂空电池分别使用空气中的氧气和金属锂作为正负极活性材料，具有极高的能量密度。但是，这一体系尚不能实现商业化的应用，其中一些关键问题未能解决。由于其正极活性材料是气体，使得电化学反应涉及气-液-固三相界面，电极过程十分复杂。与其它二次电池相比，空气电极需要考虑结构因素和催化因素。不仅要改善氧气电化学反应的动力学迟缓问题，还要考虑放电产物的驻留空间问题。董全峰教授课题组在前期开展了基于空气电极固相表面电催化研究，并结合电极结构方面的问题，构筑了有利于氧气发生反应的仿生开放式结构电极。 该研究工作主要由化学化工学院2015级iChEM直博生林晓东（第一作者）在董全峰教授、郑明森副教授和龚磊副教授的共同指导下完成，理论计算由袁汝明助理教授（共同第一作者）完成，曹勇、丁晓兵、蔡森荣、韩博闻等学生参与了部分工作。周志有教授和洪宇浩博士生在电化学微分质谱方面给予大力的帮助与支持。【Abstract】The aprotic lithium-oxygen (Li-O2) battery has attracted worldwide attention because of its ultrahigh theoretical energy density. However, its practical application is critically hindered by cathode passivation, large polarization, and severe parasitic reactions. Here, we demonstrate an originally designed Ru(Ⅱ) polypyridyl complex (RuPC) though which the reversible expansion of Li2O2 formation and decomposition can be achieved in Li-O2 batteries. Experimental and theoretical results revealed that the RuPC can not only expand the formation of Li2O2 in electrolyte but also suppress the reactivity of LiO2 intermediate during discharge, thus alleviating the cathode passivation and parasitic reactions significantly. In addition, an initial delithiation pathway can be achieved when charging in turn; thus, the Li2O2 products can be decomposed reversibly with a low overpotential. Consequently, the RuPC-catalyzed Li-O2 batteries exhibited a high discharge capacity, a low charge overpotential, and an ultralong cycle life. This work provides an alternative way of designing the soluble organic catalysts for metal-O2 batteries.This work was supported by the National 973 Program (2015CB251102), the Key Project of National Natural Science Foundation of China (21673196, 21621091, 21703186, 21773192),and the Fundamental Research Funds for the Central Universities (20720150042,20720150043). The authors thank Prof. Eric Meggers at Philipps-Univeristaet Marburg for his discussion about the synthesis of RuPC complex; Prof. Gang Fu at Xiamen University for his instructive discussions in DFT calculations; Lajia Yu and Dandan Tao at Xiamen University for their assistance in EPR experiments and UV-Vis spectroscopy experiments, respectively; and Yu Gu and Tao Wang at Xiamen University for their discussions in XPS results and CV data,respectively. 该工作得到科技部重大基础研究计划（项目批准号：2015CB251102）、国家自然科学基金（项目批准号：21673196、21621091、21703186、21773192）和中央高校基本科研业务费专项资金（项目批准号：20720150042、20720150043）的资助。 此外，感谢傅钢教授在理论计算方面的讨论和建议，Eric Meggers教授在配合物合成上的讨论，泉州师范学院吴启辉教授和化学化工学院谷宇博士生在X射线光电子能谱方面的帮助，于腊佳老师在电子顺磁共振实验上的帮助，陶丹丹博士生在紫外可见光谱测试上的帮助以及王韬博士生在循环伏安方面的讨论