评价现场混装炸药爆热稳定性的实验研究

为了在现场条件下了解孔内混装炸药的做功能力,根据水下爆炸气泡脉动周期与炸药爆热之间的定量关系,提出一种快速、方便地评价炸药爆热稳定性的方法。取20?30克现场混装炸药,置于盛满水的直径2.0米、高1.6米的圆柱形金属桶中,以6发雷管起爆炸药,通过PVDF膜片测试水中自由场压力,得到水下爆炸气泡脉动周期。测试表明,对同一批次的炸药,5次测试的周期值离散度低于3%,相应地爆热值的离散度低于10%。文中所建立的爆热稳定性测试方法有可靠的精度,所需设备及操作方法简易,适于在现场应用

液态CO_2多致裂管爆破同步性及爆破效果优化试验研究

现场进行爆破作业时,为提升爆破效果,往往布设多个钻孔,同时起爆。实际上,多致裂管起爆的时间差较大,同步性较难控制,直接影响爆破效果。本文提出一种提升液态CO2多致裂管爆破同步性方法,保持现有致裂管主体结构不变,将药包直接粘贴在防爆片上。通过开展致裂管爆破对比试验,对爆破全过程进行压力数据采集。经数据对比分析发现,原有致裂管内药包与防爆片分置在致裂管两端,药包点燃后,需通过致裂管内的液态CO2传递压力,击破防爆片共需30-60ms,优化后不足20ms,可明显降低爆破时间的不确定性,这种条件下多管串并联爆破更容易实现同步性。另外,通过开展对比试验研究,在其他条件不变的前提下,增大致裂管出气口面积20%,可有效增大总冲量值10%,提升爆破效果

Feasibility study on contact stiffness acquisition by hammering tests and numerical back-analysis for parallel structural plane of rock mass

针对相对均匀的平行结构面,基于结构面的线性接触假设,提出了一种利用锤击试验及数值反演获得结构面接触刚度的方法。该方法实施时,在结构面两侧分别布设若干支振动传感器,在远处利用激震锤进行激震,通过结构面两侧垂直于结构面方向和平行于结构面方向振动传感器的起跳时间,计算出纵波和横波通过结构面的耗时,而后利用数值计算进行反分析,通过不断调整结构面的法向和切向接触刚度,实现与现场实测相同的耗时,从而获得该结构面的法向和切向接触刚度特性。对该测试方法的可行性进行了理论、数值方面的论证,结果表明纵波过缝耗时仅受法向接触刚度控制,横波过缝耗时仅受切向接触刚度控制,并存在一一对应的关系。而后,通过对花岗岩岩块的试验及数值分析,证明了通过锤击试验快速测试岩体结构面接触刚度的方法是可行的,且具有较好的稳定性

1,3-取代吲唑类低氧诱导因子l抑制剂的设计合成及其抗肝癌活性

低氧诱导因子l（HIF-1）与肿瘤细胞的生长、侵袭和耐药密切相关,在肿瘤细胞内HIF-1高度表达,因此新型的HIF-1抑制剂可作为潜在的抗肿瘤药物。本文合成了9个1,3-取代吲唑衍生物。通过蛋白质印迹（Western Blot）法及实时定量荧光PCR（Real time-PCR）等方法检测了其对HIF-1及其靶基因血管内皮生长因子（VEGF）表达水平的影响,并以3-（5’-羟甲基-2’-呋喃基）-1-苯甲基吲唑（YC-1）为阳性对照药物初步评价了其体外抗肝癌细胞增殖的生物活性。实验发现化合物7b可显著抑制HIF-1及其下游靶基因VEGF的表达,且体外抗肝癌增殖生物活性优于YC-1,半抑制浓度(IC50)值为10.37μmol/L。研究结果表明,3-（5’-羟甲基-2’-呋喃）-1-（1″-对甲苯磺酰基）吲唑具有靶向抑制HIF及良好的抗肝癌活性作用。福建省科技厅项目(2015Y0081,2015J01350);;厦门大学大学生创新创业训练计划项目(2016X0644,20720152005)~

PURIFICATION of P49 ANTIGEN GENE PRODUCT of TRICHINELLA SPIRALLS EXPRESSED IN ESCHERICHIA COLI

目的建立旋毛虫P49抗原基因原校表达产物的纯化方法。方法菌体经冻融、超声破菌及提取包涵体后，用离子交换和凝胶过滤层析纯化蛋白质。结果纯化后的融合蛋白P49/gST经SdS-PAgE电冰鉴定达电泳纯。双抗体夹心ElISA法检测结果表明纯化的P49/gST融合蛋白能与旋毛虫感染的鼠血清和纯化融合蛋白免疫的兔血清反应，而不与正常民和兔血清反应。结论纯化后的融合蛋白P49/gST具有较高的纯度及较强的免疫活性，可望作为旅毛虫病的免疫诊断抗原。Aim To establish a purification procedure for p49 antigen gene product of Trichinella spiralis expressed inEschedchia coli.Methods After centrifugation of the bacterial culture, the bacterial pellet was resuspended and lysed byfreezing-thawing treatment and ultrasonication.Ion exchanged and gel filtration chromatography were used to purify the fu-sion protein p49/GST from the inclusion bodies- Re8ultS The purity of the fusion protein p49/GST was verified using SDS-PAGE.The purified p49/GST protein could react with mouse antisera against Tricinella sPiralis and rabbit antisera againstpurified p49/GST protein, but not with normal mouse and rabbit sera by sandwich EL1SA.Concluslon The purified fusionprotein p49/GST with high purity and good immune activity could be used for the immune aasay of trichinellosis.福建省重点（农医）项目!95－Z－15

皱纹盘鲍内脏脂质对人肝癌细胞HepG2脂质调节作用研究

本研究探讨了皱纹盘鲍(Haliotis discus hannai)内脏脂质(Haliotis discus hannai visceral lipid,HDHL)对人肝癌细胞HepG2的脂质代谢的影响,对HDHL脂肪酸成分、HepG2细胞活性、细胞内胆固醇和甘油三酯含量以及脂肪...海洋公益性行业科研专项(201405016

Experiment on micron-sized particle production of iron ore by rapid unloading of liquid CO2

The average iron content of iron ore is &lt;30%; therefore, crushing, grinding, milling and other processing techniques must be executed before smelting. Currently, it is expensive to break iron ores using mechanical grinding. Experiments have been carried out to develop a novel approach of producing iron ore powder. First, the iron ore is placed in a high-pressure chamber, and then liquid CO2 is injected into the chamber. Second, considering energy recycling, after the iron ore pores are filled with liquid CO2, dissociative liquid CO2 is substituted and collected for cyclic utilization. Third, an initial high pressure is applied inside the chamber using a water pump in order to increase the energy input. Fourth, the pressure is rapidly unloaded. After penetration and gasification expansion, the iron ore will immediately be converted into micron-sized particles. Laser grain size analysis indicated that the grain size of the iron ore particles ranges between 30 and 50 pm, which will satisfy the requirements of gravity separation, magnetic separation and the flotation process. This is a highly efficient and low-cost method that has excellent industrial promotion value. (C) 2017 Elsevier B.V. All rights reserved.</p

皱纹盘鲍内脏脂质对人肝癌细胞HepG2脂质调节作用研究

本研究探讨了皱纹盘鲍(Haliotis discus hannai)内脏脂质(Haliotis discus hannai visceral lipid,HDHL)对人肝癌细胞HepG2的脂质代谢的影响,对HDHL脂肪酸成分、HepG2细胞活性、细胞内胆固醇和甘油三酯含量以及脂肪酸代谢相关mRNA基因的表达情况进行了研究。研究发现HDHL中不饱和脂肪酸占总脂肪酸含量59.50%,其中多不饱和脂肪酸占29.71%。HDHL与HepG2细胞共孵培养24 h及48 h后,浓度为0～240μg/mL的HDHL对HepG2细胞无毒性影响。30～240μg/mL HDHL可显著降低HepG2总胆固醇含量,30～120μg/mL HDHL共孵HepG2细胞后,细胞内甘油三酯含量显著降低。qPCR结果显示HDHL可显著降低HepG2细胞脂肪酸合成相关基因SREBP1c、ACC1、FAS和脂肪酸转运吸收相关基因CD36 mRNA水平的表达,提高线粒体脂肪酸氧化基因CPT1的表达。因此,HDHL可能通过抑制脂肪酸合成和脂肪酸转运,增强线粒体中脂肪酸氧化等途径有效调节细胞脂质代谢,该研究为脂质调节功能食品的开发提供理论依据。海洋公益性行业科研专项(201405016

经内窥镜大隐静脉获取术在冠状动脉旁路移植术中应用的学习曲线

目的探讨内窥镜获取大隐静脉在冠状动脉旁路移植术中的应用并探讨学习曲线，重点关注初学者易发生的问题及对早期临床结果的影响。方法回顾性分析2013年7月至2014年4月在郑州大学第一附属医院心外科接受不停跳冠状动脉旁路移植术并使用内窥镜技术获取大隐静脉的83例患者的临床资料，按照手术时间的先后顺序分为A组（初学组20例）、B组（熟悉组20例）、C组（进步组20例）、D组（成熟组23例），分析各组之间患者围术期及随访结果差异，明确学习曲线周期。结果该组患者年龄为（60.22±8.06）岁，体质量为（69.77±11.66） kg，其中合并高血压24例、糖尿病26例、亚急性脑梗14例。A组相对于后三组获取大隐静脉长度与时间比值明显较小（P&lt;0.001），静脉主干损伤数量明显较多（P=0.006），并且随访1年时静脉桥通畅率较低，但差异无统计学意义。结论内窥镜获取大隐静脉之前技术操作培训是必要的，能有效规避初学者造成的血管损伤，实际获取的过程中大概需要亲自操作20例，并认真总结技术技巧就可以较为熟练地进行相关操作

Antioxidant enzymes from the crab Scylla paramamosain: Gene cloning and gene/protein expression profiles against LPS challenge

Recent studies revealed that antioxidant enzymes play important roles in antioxidant responses caused by metabolic process or pathogen invasion. Catalase is one of these key enzymes which has been characterized and highly conserved from invertebrates to vertebrates. In the present study, a full-length cDNA sequence of catalase was cloned from the hemocyte suppression subtractive hybridization library of the crab Scylla paramamosain. The Sp-catalase (Sp-CAT) cDNA sequence contained 2551 bp with an open reading frame of 1551 bp encoding 517 amino acid residues. The conserved catalytic active residues His-71, Asn-144 and Tyr-354 were predicted in the amino acid sequence of Sp-CAT. The deduced Sp-CAT protein had a calculated molecular mass of 59 kDa with an estimated isoelectric point of 6.4. Multiple alignment analysis revealed that the deduced amino acid sequence of Sp-CAT shared high identity (75.4%) with those of other species. The Sp-CAT mRNA transcripts were demonstrated in multiple tissues of normal S. paramamosain. After LPS challenge, the expression level of Sp-CAT gene was increased significantly in hemocyte at 3 and 6 h, and in hepatopancreas at 6 h, respectively, determined by quantitative real-time PCR. Furthermore, the activities of CAT and SOD were also measured in different tissues and serum after LPS challenge. The CAT activity was significantly increased at 3, 6, 24 and 48 h in hemocyte lysate, at 3 h in serum, and at 24 and 48 h in hepatopancreas after LPS challenge. In addition, the SOD activity was significantly induced at 3 and 6 h in hemocyte lysate, 3 and 12 h in serum, 12 and 48 h in hepatopancreas post LPS stimulation, indicating a tissue and time-dependent antioxidant response in the crab. Taken together, these data demonstrated that a strong antioxidant response occurred in the LPS-challenged crab, which might be involved in the protection of host against microbial infections. (C) 2010 Elsevier Ltd. All rights reserved.National Natural Science Foundation of China (NSFC) [40676083]; National High Technology Research and Development Program of China [2007AA091406]; Minjiang Scholars Program [2009]; Natural Science Foundation of Fujian Province China (NSFC-FPC) [2009J05084