51 research outputs found

    Corrigendum

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    Evaluation of genetic diversity: Which of the varieties can be named ,Rebula' (Vitis vinifera L.)?Vitis 49 (4), 129-136 (2010

    Evaluation of genetic diversity: Which o f the v arieties can be named 'Rebula' (Vitis vinifera L.)?

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    The variety 'Rebula' is an indigenous but also ancient variety in the Slovenian and Italian world-renowned winegrowing district Collio dating back to the ages of the Roman Empire. The DNA analyses of the variety ‘Rebula’ and its potential related varieties were conducted to evaluate its biodiversity. The variety 'Rebula' showed a very low similarity (16 %) with other analysed varieties, also with 'Rebula briška' and 'Rebula-old'. The varieties called 'Prosecco', 'Števerjana', 'Beli teran' and 'Briška Glera' revealed identical genotypes in all 11 SSR microsatellites analysed, therefore are regarded as synonyms. The varieties 'Rebula' and 'Ribolla gialla' revealed the identical SSR profile at 8 out of 9 SSR loci. 'Vitovska Grganja' share with 'Vitouska' only 55 % of analysed alleles but show their first degree relationship. The DNA analysis still showed existing deviations in synonyms and homonyms linked to the variety 'Rebula' what impedes its standardisation and revitalization.

    Microsatellite genotyping of old Slovenian grapevine varieties (Vitis vinifera L.) of the Primorje (coastal) winegrowing region

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    Thirty-three grapevine varieties from Slovenia were genotyped at 21 microsatellite loci in order accurately to identify varieties and to evaluate their synonyms and homonyms, including varieties cultivated in neighbouring countries. Among Slovenian varieties some previously assumed synonyms were confirmed and some new ones were discovered: ‘Poljšakica Drnovk’ = ‘Istrska Malvazija’, ‘Pikolit Italy’ = ‘Pikolit Vienna’, ‘Vitovska grganja’ = ‘Racuk’ and ‘Prosecco’ = ‘Glera’ = ‘Števerjana’. Types of Zelen (‘Zelen Pokov’, ‘Zelen 66’ and ‘Zelen 2.4’) were distinct at several microsatellite loci so can only be considered to be homonyms. Two ‘Picolit’ types were considered to be ‘true-to-type’ on the basis of comparison with ‘Picolit’ clones from Italy. Synonymy between ‘Heunisch’ and ‘Belina’ was not confirmed in our study, since ‘Belina Pleterje’ differentiated from ‘Heunisch weiss’ at 13 out of 19 loci. Comparison of ‘Vitovska grganja’ from Slovenia with ‘Vitouska’ from Italy also showed dissimilarities at the majority of the analysed loci.

    Screening of drought oxidative stress tolerance in Serbian melliferous plant species

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    This study was designed to examine and compare antioxidant and free-radical scavenging activities of leaves of six different melliferous plant species (Populus alba, Robinia pseudoacacia, Sophora japonica, Euodia hupehensis, Tilia sp., Fraxinus sp.) from Serbia in order to evaluate their drought oxidative stress tolerance. Experiment was conducted during June, July and August. In this study, we reported the results concerning proline accumulation, soluble protein content, quantities of malonyldialdehyde,total antioxidant capacity determined by FRAP method and scavenger activity determined by DPPH method. According to our results, all melliferous plant species were subjected to drought oxidative stress during July when soil humidity decreased. During July, proline content and MDA quantity increased and soluble proteins decreased in all investigated species. High and permanent antioxidant activity during the whole investigated period was observed in P. alba, but insufficient to protect itsleaves from oxidative injury during the period of drought in July. The highest ability to accumulate proline and highest protein content under severe drought stress in July was observed in Fraxinus sp. Other investigated antioxidant parameters (total antioxidant and DPPH radical scavenger capacities) were high and accumulation of MDA was low which indicate high drought oxidative stress tolerance. Therefore, highest ability to adapt under severe drought stress and highest drought oxidative stresstolerance were observed in Fraxinus sp.Key words: Melliferous trees, lipid peroxidation, DPPH, FRAP, proline accumulation

    Israeli germplasm: phenotyping and genotyping of native grapevines (Vitis vinifera L.)

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    In this study 32 grapevine accessions from Israel were phenotyped according to O.I.V descriptors and phyllometry, furthermore genotyped with seven microsatellite loci for the first time for further clarification of their potential biodiversity and phylogenetic grapevine analysis. The results according to the SSR microsatellites suggest that the majority of the studied Israeli grapevine accessions, compared to the international reference varieties, show considerably different genotypes - nine accessions showed a unique (individual) genotype, further three accessions represented a first group, four accessions represent a second group and eight groups contained two different accessions. The significant differences among the studied grapevine accessions were observed also in most of the 17 phyllometric measurements of significant leaf characteristics, especially in the leaf area, vein length (L1, L2, L3 and L4), depth of lateral sinuses (OS, OI), petiol length (Lp) and vein angles (β, β’). The observed grapevine characteristics according to the O.I.V. descriptors suggested that more than three-quarters of the studied accessions showed characteristics of table grapes

    Ampelographic characterisation of grapevine accessions denominated 'Refošk', 'Refosco', 'Teran' and 'Terrano' (Vitis vinifera L.) from Slovenia, Croatia and Italy

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    Grapevines denominated 'Refošk', 'Refosco', 'Teran' and 'Terrano have been cultivated in the area of western Slovenia, north-western Croatia and north-eastern Italy for centuries. Despite historical documents reporting the longstanding tradition of grapevine cultivation and winemaking, the denomination and origin of these varieties is still questionable. The aims of this work were to study the genetic identity and relationship of the grapevine accessions denominated 'Refošk', 'Refosco', 'Teran' and 'Teranno' that have been traditionally cultivated in Slovenia, Croatia and Italy. For this purpose, 9 SSR loci were analysed to fingerprint 53 accessions with denominations or similar true-to-type morphologies of 'Refošk' and 'Teran'. The grapevine variety 'Refošk' cultivated in Slovenia and most accessions denominated 'Teran' in Croatia showed identical genotypes in all analysed SSR markers, and can therefore be used as synonyms. Five accessions showed identical genotypes to 'Refosco dal peduncolo rosso' variety, however five other genotyped accessions suggested individual profiles, and can be characterized as clonal mutants of true-to-type 'Refošk'/'Teran'. Accessions 'Sladki Teran' and 'Ref5/31' shared 56 % and 61 % of the alleles with true-to-type profiles of 'Refošk'/'Teran' and their parentage analysis strongly suggested a full-siblings relationship. Obtained results contribute to the understanding of the genetic diversity of grapevine varieties cultivated in this part of Europe

    Chemical parameters of oxidative stress adaptability in beech

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    e antioxidant activity, lipid peroxidation, and contents of free proline and soluble proteins were investigated on six-year-old beech plants. Provenance Avala, in Serbia, had the best adaptability to environmental factors on locality Fruska Gora due to low lipid peroxidation, high FRAP value, and free proline and soluble proteins contents. Provenances Scharnstein and Mitterndorf, in Austria, had the best adaptability to environmental factors on locality Debeli Lug due to high FRAP value and free proline and soluble proteins contents. FRAP values in majority of provenances from locality Debeli Lug were higher. Correlations parameters were much higher between provenances in locality Debeli Lug, situated at higher altitude, which is the consequence of better adaption to environmental factors in�uence

    Measurement of polarization-transfer to bound protons in carbon and its virtuality dependence

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    We measured the ratio Px/PzP_{x}/P_{z} of the transverse to longitudinal components of polarization transferred from electrons to bound protons in 12C^{12}\mathrm{C} by the 12C(e,ep)^{12}\mathrm{C}(\vec{e},e'\vec{p}) process at the Mainz Microtron (MAMI). We observed consistent deviations from unity of this ratio normalized to the free-proton ratio, (Px/Pz)12C/(Px/Pz)1H(P_{x}/P_{z})_{^{12}\mathrm{C}}/(P_{x}/P_{z})_{^{1}\mathrm{H}}, for both ss- and pp-shell knocked out protons, even though they are embedded in averaged local densities that differ by about a factor of two. The dependence of the double ratio on proton virtuality is similar to the one for knocked out protons from 2H^{2}\mathrm{H} and 4He^{4}\mathrm{He}, suggesting a universal behavior. It further implies no dependence on average local nuclear density

    Fuzzy modelling of tourist motivation: An age-related model for sustainable, multi-attraction, urban destinations

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    Tourist motivation, as a core of travel behavior, significantly influences consumer intentions and has attracted academic attention for decades. A plethora of studies analyse sets of internal and external motivators, while methodologies that exclusively focus on a single factor, such as age, that can sometimes have a determining influence in multi-attraction destinations, are less prevalent. This study introduces a fuzzy logic approach to develop a new model for analysing the internal motivations of different-aged consumers in multi-attraction urban destinations. Fuzzy models, as a mathematical means of representing vagueness and imprecise information, have the capability of recognizing, representing, manipulating, interpreting, and utilizing data and information, which typically for urban tourist motivations, are vague and lack certainty. This research tests the model in a real-life setting, using the example of Novi Sad, a mid-sized European city, which is typical of many similar cities who are attempting to develop sustainable tourism by attracting older tourists. The new model shows how tourist motivations for multi-attraction destinations are affected by age, through a specially developed m-file for MATLAB, so that it can be applied and tested in other tourism contexts. Theoretical and practical implications for sustainable destination management and marketing are described
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