Immunocytochemical analysis of bifid trichomes in Aldrovanda vesiculosa l. traps

The two-armed bifids (bifid trichomes) occur on the external (abaxial) trap surface, petiole, and stem of the aquatic carnivorous plant Aldrovanda vesiculosa (Droseracee). These trichomes play the role of mucilage trichomes. This study aimed to fill the gap in the literature concerning the immunocytochemistry of the bifid trichomes and compare them with digestive trichomes. Light and electron microscopy was used to show the trichome structure. Fluorescence microscopy revealed the localization of carbohydrate epitopes associated with the major cell wall polysaccharides and glycoproteins. The stalk cells and the basal cells of the trichomes were differentiated as endodermal cells. Cell wall ingrowths occurred in all cell types of the bifid trichomes. Trichome cells differed in the composition of their cell walls. The cell walls of the head cells and stalk cells were enriched with arabinogalactan proteins (AGPs); however, they were generally poor in both low- and highly-esterified homogalacturonans (HGs). The cell walls in the trichome cells were rich in hemicelluloses: xyloglucan and galactoxyloglucan. The cell wall ingrowths in the basal cells were significantly enriched with hemicelluloses. The presence of endodermal cells and transfer cells supports the idea that bifid trichomes actively transport solutes, which are polysaccharide in nature. The presence of AGPs (which are considered plant signaling molecules) in the cell walls in these trichome cells indicates the active and important role of these trichomes in plant function. Future research should focus on the question of how the molecular architecture of trap cell walls changes in cells during trap development and prey capture and digestion in A. vesiculosa and other carnivorous plants

Morphology and ultrastructure of the midgut in Piscicola geometra (Annelida, Hirudinea)

This paper presents information on the organization of the midgut and its epithelium ultrastructure in juvenile and adult specimens of Piscicola geometra (Annelida, Hirudinea), a species which is a widespread ectoparasite found on the body and gills and in the mouth of many types of fish. The analysis of juvenile nonfeeding specimens helped in the explanation of all alterations in the midgut epithelium which are connected with digestion. The endodermal portion (midgut) of the digestive system is composed of four regions: the esophagus, the crop, the posterior crop caecum, and the intestine. Their epithelia are formed by flat, cuboidal, or columnar digestive cells; however, single small cells which do not contact the midgut lumen were also observed. The ultrastructure of all of the regions of the midgut are described and discussed with a special emphasis on their functions in the digestion of blood. In P. geometra, the part of the midgut that is devoid of microvilli is responsible for the accumulation of blood, while the epithelium of the remaining part of the midgut, which has a distinct regionalization in the distribution of organelles, plays a role in its absorption and secretion. Glycogen granules in the intestinal epithelium indicate its role in the accumulation of sugar. The comparison of the ultrastructure of midgut epithelium in juvenile and adult specimens suggests that electron-dense granules observed in the apical cytoplasm of digestive cells take part in enzyme accumulation. Numerous microorganisms were observed in the mycetome, which is composed of two large oval diverticles that connect with the esophagus via thin ducts. Similar microorganisms also occurred in the cytoplasm of the epithelium in the esophagus, the crop, the intestine, and in their lumen. Microorganisms were observed both in fed adult and unfed juvenile specimens of P. geometra, which strongly suggests that vertical transmission occurs from parent to offspring

Multiple allosteric effectors control the affinity of DasR for its target sites

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Podstawy teoretyczne koncepcji WAZO, cz. 1

W serii Metody Organizacji i Zarządzania (MOiZ) publikowane są cyklicznie opracowania, których celem jest upowszechnienie metod (technik, narzędzi) z zakresu organizacji i zarządzania oraz ułatwienie ich transferu z nauki do biznesu. Seria jest wydawana w ramach Centrum Metod Organizacji i Zarządzania działającego na Wydziale Zarządzania Uniwersytetu Łódzkiego.Metodyka WAZO powstała w ramach projektu finansowanego ze środków Narodowego Centrum Nauki przyznanych na podstawie decyzji numer DEC-2013/09/B/HS4/02722

Alcohol in minors - a harmless fad or a serious problem?

Introduction and purpose: Alcohol has been known for centuries. In recent years, awareness of the effects of its consumption has been growing and acceptance of the drink has been declining. Despite this, there is an increase in young alcohol consumption and a decrease in the age of alcohol initiation. In this work, the problem of alcohol consumption by minors will be reviewed based on the evaluation of hospitalizations in the Clinical Department of Toxicology and Cardiology of the Stefan Cardinal Wyszynski Regional Hospital in Lublin in 2022. The aim of the study is to draw attention to this important, although often downplayed, problem. Material and method: The study was retrospective in nature. It used an analysis of the records of 120 intoxications in people under the age of 18, from which a group of alcohol-poisoned people was distinguished, consisting of 37 cases. Results: Hospitalization of 7 patients aged 13 to 18 years was reported due to alcohol intoxication. Of these, 22 (59.5%) female and 15 (40.5%) male patients were singled out. Most were urban residents 29 (78.4%). Isolated alcohol intoxication was the cause of 18 hospitalizations(48.6%). The remaining cases were accompanied by drug intoxication 11 hospitalizations (29.7%) and drug intoxication 8 hospitalizations (21.6%). All analyzed intoxications were intentional, of which 9 (24.3%) were suicide attempts. In 14 cases (37.9%), patients were burdened with mental illness, the most common of which was depressive disorders. There were no deaths in the study group. Conclusions: Alcohol should be forbidden and unacceptable in the family and young people's environment. Parents and young people should be made aware of the harmful effects, psychological, social and health consequences of its consumption

Analysis of poisonings in patients up to 18 years old hospitalized in the Clinical Department of Toxicology and Cardiology in Stefan Wyszyński Regional Specialist Hospital in Lublin in 2022

Introduction and purpose: Poisoning in children and adolescents remain a serious medical, psychological and social problem. The aim of the study was to analyse poisonings in patients up to 18 years old admitted to the Clinical Department of Toxicology and Cardiology in Stefan Wyszyński Regional Specialist Hospital in Lublin in 2022. Material and method: The research was retrospective. Medical records of 120 cases of poisoning in patients up to the age of 18 were analysed. Results: In the studied period 120 patients from 13 to 18 years old were hospitalized due to intoxication,  84 (70%) of them were female and 36 (30%) of them were male.  Majority of  cases came from urban areas (69,2%). The most frequent causes of poisonings were medicaments (55%), drugs (29,2%) and alcohol (28,3%). The intentional intoxications were found in 113 (94,2%) cases, 45 (39,8%) of them were suicide attempt. Mental diseases were found in 71 (59,1%) cases, the most common one was depressive disorder. There were no deaths in the researched group. Conclusions: Because of the relevance of child patients poisoning young people should be provided with psychological care. Female teenagers must get special kind of support. The availability of child psychiatrists and psychologists is still too poor. Prohibition of selling alcohol to minors should be enforced stricly. It seems that acces to OTC drugs is way much too easy for people. There is a necessity of parents’education about children poisoning

Premenstrual syndrome - theories and facts - something everyone should know

Premenstrual syndrome (PMS- Premenstrual Syndrome) is a group of cyclic somatic or psychological symptoms occurring during the luteal phase of the cycle and disappearing with the onset of menstruation. The purpose of this work is to draw attention to this important but often underestimated problem, trace its pathomechanism and identify treatment methods. The interaction of many factors and predispositions is responsible for the occurrence of PMS/PMDD (PMDD- premenstrual dysphoric disorder). One of the most important is ovulatory cycles and cyclically repeated changes in ovarian hormone secretion- fluctuations in progesterone, estradiol and prolactin levels during the various phases of the cycle. The association of emotional disturbances, severe stress and disturbances in the neurotransmitter system of the central nervous system, as well as many other factors, mentioned in the article below, is described. Various methods are used to treat PMS/PMSS, ranging from less to more invasive, including lifestyle changes, cognitive-behavioral therapy, and pharmacotherapy and surgical treatment.  Currently, SSRIs-serotonin reuptake inhibitors-are considered the main therapy, as well as hormonal treatment-the two-pill. A review of the literature shows that PMS/PMDD is a real problem that reduces quality of life and has a negative impact on family and social relationships. Increased public awareness and interest by physicians of many specialties in this problem will enable faster diagnosis and implementation of effective therapy, thereby minimizing the negative social impact

Taxonomic classification of algae by the use of chlorophyll a fluorescence

Natural water reservoirs are very important ecosystems thus they should be under continuous monitoring and protection. In water of low quality, the algal blooms develop develops vastly. The knowledge of algal species composition is necessary for understanding this process. There are a few traditional group-specific methods of microalgae classification, but they are often labour-intense and time-consuming. Moreover, the samples must be prepared and/or collected before getting any results. Non-invasive chlorophyll fluorescence analysis offers an alternative approach and potentially allows in situ estimation of algal concentration. In this work the fluorometric methods to estimate algae content in water and to differentiate algal populations is presented

Konsekwencje zmian dla zachowań organizacyjnych

Przyjęcie zróżnicowanych perspektyw w procesie analizy pozwala autorkom na sformułowanie wniosków na temat najbardziej symptomatycznych konsekwencji zmian charakterystyki organizacji, które z pewnością nie wyczerpują całości zagadnienia, pozwalają jednak na ukazanie kierunku, w jakim ewaluują zachowania ludzi w organizacji.Udostępnienie publikacji Wydawnictwa Uniwersytetu Łódzkiego finansowane w ramach projektu „Doskonałość naukowa kluczem do doskonałości kształcenia”. Projekt realizowany jest ze środków Europejskiego Funduszu Społecznego w ramach Programu Operacyjnego Wiedza Edukacja Rozwój; nr umowy: POWER.03.05.00-00-Z092/17-00