329 research outputs found
Comparison of Participation Constraints in Recreational Physical Activity of the Teachers Working Iğdir and Erzurum Provinces
The aim of the study is to compare the participation constraint for leisure activities of teachers who working In the Ministry of National Education (MNE). In order to determine the leisure time constraints leisure constraints scales (LCS) were applied to the participants. A total of 413 teachers (258 male and 155 female) working in the Ministry of National Education participated in the study in Iğdır and Erzurum provinces. No statistically significant difference was found at the level of p <0,05 in any of the sub-dimensions as a result of the inter-city comparison of the female participants. While there was a significant difference in the lack of information, facilities and time sub-dimensions of the male participants in the study, p <0.05 was not significant difference in the other sub-dimensions. It is seen that the difference is in favor of the participants from the province of Erzurum. There was no statistically significant difference between the two cities in terms of lack of information, facilities and time (p <0,05) in the subscales, as in male participants. The female teachers who working Iğdır and Erzurum province there was no difference between the scores in the LCS and the mean scores of both groups were close to each other. For the male participants working in Iğdır province, it was determined that the lack of information, facilities and time constraints were more effective in participation of recreational physical activities than male participants working in Erzurum province
A computational approach to decipher chromatosome structure determinants
In eukaryotic cells, DNA transcription, replication and repair events are controlled by the regulation of DNA compaction mechanisms that determine the open and closed chromatin states. Nucleosomes are the basic DNA packaging units of chromatin. The nucleosome core (NC) consists of a core histone protein octamer with approximately two tight superhelical turns of DNA wrapped around it. The NC is extended at its entry and exit points by linker DNA (L-DNA) and a linker histone (LH) protein binds between the two L-DNA arms to form a chromatosome. The dyad is the single DNA base pair between the nucleosome entry and exit points determining the symmetry axis and is used to define the position of LH binding to a nucleosome. For LH - nucleosome binding, previous studies indicate both on- and off-dyad binding modes, as well as different LH orientations. Thus, the molecular determinants of the structure of LH – nucleosome complex and the dynamics of LH – nucleosome binding are not fully understood.
The aim of the research described here was to obtain an atomic-detail level understanding of chromatosome formation. Analysis of the experimentally determined structures of LH – nucleosome complexes showed that instead of a single 3D structure, an ensemble of structures of LH – nucleosome complexes exists. To understand the distribution of these ensembles, normal mode analysis (NMA), standard and accelerated molecular dynamics (MD & AMD) and Brownian dynamics (BD) simulations were applied to LH, nucleosome and chromatosome systems. MD and AMD simulations showed that the globular domain of the LH (LH GD) prefers to be in its closed form in solution. Upon nucleosome binding, the LH GD structure transformed to an open structure due to hydrophobic interactions with the L-DNA of the nucleosome. Additionally, LH GD binding constrained the flexibility of the L-DNA and affected the directions of movement of the L-DNA arms. BD simulations indicated that various chromatosome configurations were possible depending on LH GD sequence and L-DNA opening angles. These findings suggest that LH – nucleosome binding is mediated by a combination of conformational selection and induced fit mechanisms.
Further BD simulations show that chromatosome configurations were affected by single point mutations in the LH GD and varied for different LH isoforms. My results indicate that by making specific single point mutation exchanges, it is possible to swap LH – nucleosome configurations among different LH GD isoforms. Similar shifts were observed in chromatosome configuration upon introduction of post translational modifications (PTMs) in the LH GD.
I applied BD simulations to compute dissociation rate constant (koff) values and compare them with previously reported fluorescence recovery after photobleaching (FRAP) data on the binding of various LH mutants to chromatin. The results of the BD simulations correspond with the relative trends in measured FRAP recovery half-times (t50) of LH – chromatin binding of various LH
mutants. The results thus enable the interpretation of the FRAP data in terms of a physical model of LH – nucleosome binding.
My thesis provides detailed insights into the structure, dynamics and kinetics of chromatosome formation in eukaryotes. The results presented in this work can guide further experiments on the sequence determinants of LH – nucleosome binding
Effects of Long Term Volleyball Training on Mineral Metabolism and Hematological Parameters
Objective: Following acute exercises, changes in mineral and hematological concentrations are
observed in the organism depending on the exercise, but regular and submaximal exercises do not
fully reveal the mineral and hematological effects. This study was conducted to determine the effects
of volleyball training on mineral metabolism and hematological parameters over a period of three
months.
Method:The research group included 20 healthy male volunteers in the 13-16 age group. A training
program of 80 minutes per day, 4 days per week, for three months was applied to the athletes who
participated in the study. Blood samples were taken twice before and at the end of the training
sessions from the resting athletes. Sodium, potassium, chlorine, calcium, magnesium, WBC, RBC,
HGB, HCT, MCV, MCH, MCHC, PLT, MPV and PDW levels were determined in the blood samples
collected. The data were analyzed using the SPSS 22 package program. The Paired Samples t-test was
used to compare the research group's pre-final test data. Significance was considered to be p>0.05.
Finding: The analysis showed that the research group had statistically significant differences in
sodium, chlorine, phosphorus, magnesium, RBC, HCT, MCV, MCH, MCHC, MPV and PCT levels
(p<0.05) and no statistically significant differences in potassium, calcium, WBC, HGB, PLT, RDW-CV
and PDW levels (p>0.05).
Conclusion: As a result, volleyball training over a long period of time has been observed to cause
changes in mineral metabolism and some hematological parameters. It was found that the regular
training schedule caused differences in some mineral and whole blood values. In the light of this
information, we believe that long-term adolescent training will have a positive impact on the health of
athletes
Problems in the participatory approach for the watershed management: an evaluation related to the forestry sector
Bu bildiride, ormancılık sektörüyle doğrudan ilişkili olan havza yönetimi sürecinde katılımcı planlamaya geçiş ve planlamanın etkin bir şekilde yürütülmesinde yaşanan sorunların değerlendirilmesi amaçlanmaktadır. Bu amaçla öncelikle havza yönetimi kavramı ve orman kaynakları yönetimiyle olan ilişkisi üzerinde durulmaktadır. Ardından, Ülkemiz ormancılık sektörünün diğer sektör ve kurumlarla olan etkileşimi; özellikle kırsal kalkınma ve ormancılık faaliyetleri planlama çalışmaları kapsamında ele alınarak irdelenmektedir.In this paper, it is aimed that the factors which are effective on the passing into participatory planning in watershed management process and its implementation effectively are evaluated. For this purpose, firstly watershed management concept and its relations with the management of forest resources will be reviewed and then the interaction of forestry sector with other sectors, rural development and forestry activities will be evaluated in the scope of planning activities
The Role of EREG, PTPN1, and SERPINB7 Genes in the Pathogenesis of Psoriasis: May SERPINB7 Be Protective and a Marker of Severity for Psoriasis?
Introduction: Psoriasis is a chronic inflammatory dermatological disease with complex pathogenesis in which many immune system cells, including keratinocytes, play a role. Many genes regulate the proliferation of keratinocytes and other immune cells that have essential roles in the pathogenesis of psoriasis.The expressions of EREG, PTPN1, and SERPINB7 genes were shown as upregulated in psoriatic skins in a few studies previously.
Objectives: We aimed to evaluate the expressions of these genes in psoriatic lesional skin and com-pared them with non-lesional adjacent skin of the same patients and normal skin of healthy controls.
Results: Our results revealed that the expressions of EREG and PTPN1 genes were upregulated,whereas the SERPINB7 gene expression was down regulated in the psoriatic skin of the patients than normal skin of controls. Moreover, the expression level of the SERPINB7 gene was also negatively correlated with the severity of the disease among patients.
Conclusions: According to our results, overexpression of EREG and PTPN1 genes, and decreased expression of SERPINB7 gene may lead to the development of psoriasis
İnfektif endokarditin epidemiyolojik, klinik ve prognostik sonuçları: 90 atağın retrospektif kohortu
To evaluate the epidemiological, clinical, microbiological, and echocardiographic features, as well as the prognosis and long- term outcome of patients with infective endocarditis. Methods: The clinical records and follow-up data of 90 endocarditis episodes in 86 patients diagnosed with definite and possible infec- tive endocarditis according to the modified Duke criteria in a tertiary university hospital, between 1998 and 2016, were reviewed. Results: Fifty-six patients were male (65.1%), and the mean age was 49.9 ± 14.3. Native valve endocarditis constituted 62.2% of the cases, while the remaining patients had prosthetic valve endocarditis. The aortic (34.4%) and mitral (24.4%) valves were infected more frequently. Streptococci (27.7%) and staphylococci (24.4%) were the most frequently isolated microorganisms. Embolic complications (35.5%) were the leading cause of morbidity, followed by valve insufficiency (28.8%) and heart failure (21.1%). Valve replacement surgery was performed in 28 patients (31%). The in-hospital mortality rate was 15.1% (n = 13). Chronic renal failure (P = .042) and degenerative valves (P = .036) were significantly associated with mortality. Among 43 of the 73 cases available for telephonic survey, 36 (83.7%) patients were alive and without disease, with a median follow-up of 52.9 (4-163) months. Twenty-five (69.4%) of these patients were younger than 55 years, and 24 (66.6%) had native valve endocarditis. Conclusion: Underlying cardiac conditions and chronic renal failure increase mortality in infective endocarditis, regardless of the patho- gen. Long-term survival seems promising in cases with native valve endocarditis and in younger patients with low rates of comorbidities.İnfektif endokardit tanısıyla izlenen hastaların epidemiyolojik, klinik, mikrobiyolojik, ekokardiyografik özellikleri, prognozu ve uzun dönem sonuçlarını değerlendirmek. Yöntemler: 1998 ve 2016 yılları arasında bir üniversite hastanesinde modifiye Duke kriterlerine göre kesin ve olası infektif endokardit tanısı konan 86 hastanın 90 endokardit atağının klinik kayıtları ve takip verileri retrospektif olarak incelendi. Bulgular: Çalışmamızda hastaların 56’sı (%65,1) erkek ve ortalama yaş 49,9 ± 14,3 idi. Doğal kapak endokarditi olguların %62,2’sini oluştururken, diğerlerinde protez kapak mevcuttu. En sık aort (%34,4) ve mitral (% 24,4) kapak tutulumu saptandı. Etken olarak strep- tokoklar (%27,7) ve stafilokoklar (%24,4) en sık izole edilen mikroorganizmalardı. Tüm komplikasyonlar içinde embolik komplikasyonlar (%35,5) ilk sırada yer almış, bunu kapak yetmezlikleri (%28,8) ve kalp yetmezliği (%21,1) izlemiştir. Hastaların 28’ine (%31) kapak replasman operasyonu yapılmış ve tüm hastalar içinde 13 hasta (%15,1) hastane takibinde kaybedilmiştir. Mortalite kronik böbrek yetmezliği (P = ,042) ve dejeneratif kapak hastalarında (P = ,036) istatistiksel olarak daha anlamlı bulundu. Taburculuk sonrası prognoz ve uzun dönem sonuçlar değerlendirildiğinde sağ kalan 73 hastanın 43’üne ulaşılabilmiş ve bu hastalardan 36’sının (%83,7) ortalama 52,9 ay (4-163) hayatta ve genel durumlarının iyi olduğu, 25 hastanın (%69,4) <55 yaş, 24’ünün (%66,6) doğal kapak endokarditi tanısıyla izlenmiş olduğu görülmüştür. Sonuç: İnfektif endokardit hastalarında altta yatan kalp hastalıkları ve kronik böbrek yetmezliği patojenden bağımsız olarak mortalit- eyi arttırmaktadır. Doğal kapak endokarditi ve komorbiditeleri daha az olan genç yaş hastalarda ise uzun dönem sağkalım umut verici görünmektedir
The role of coronary CT angiography in diagnosis of patent foramen ovale
PURPOSE:We aimed to examine the incidence of patent foramen ovale (PFO) and atrial septal aneurysms (ASA) in the Turkish population using coronary computed tomography angiography (CTA); assess the feasibility of coronary CTA for PFO diagnosis by conducting a comparison with transthoracic echocardiography (TTE); and determine the diagnostic role and characteristics of the interatrial tunnel, free flap valve (FFV), and shunts.METHODS:The present study was conducted retrospectively and included a sample of 782 patients. Coronary CTA results for all patients were evaluated for the following parameters: the presence of PFO, the degree of contrast jet (if present due to PFO), ASA existence, free flap valve (FFV) length, and PFO tunnel diameters (1 and 2). Coronary CTA and TTE results for PFO detection were also compared for 19 patients who underwent both procedures.RESULTS:PFO was present in 118 patients (15%). In 19 patients who underwent both CTA and TTE, the shunt was present in 15 patients on TTE compared with nine patients on CTA. The sensitivity and specificity of CTA for shunt existence were 53% (8/15) and 75% (3/4), respectively. FFV was observed on CTA in 118 patients (15%). No significant relationship was observed between shunt existence and FFV length (P = 0.148), or between shunt existence and tunnel diameter-1 (P = 0.638) or diameter-2 (P = 0.058). ASAs were present in 16 patients (2%), while accompanying PFO was present in three patients (2.4%) .CONCLUSION:Coronary CTA constitutes a more practical and efficient alternative to TTE for PFO diagnosis. Further, it allows the clear visualization of anatomical details of the interatrial tunnel, shunts, and associated abnormalities and detects ASAs
Putative second hit rare genetic variants in families with seemingly GBA-associated Parkinson's disease
Rare variants in the beta-glucocerebrosidase gene (GBA1) are common genetic risk factors for alpha synucleinopathy, which often manifests clinically as GBA-associated Parkinson's disease (GBA-PD). Clinically, GBA-PD closely mimics idiopathic PD, but it may present at a younger age and often aggregates in families. Most carriers of GBA variants are, however, asymptomatic. Moreover, symptomatic PD patients without GBA variant have been reported in families with seemingly GBA-PD. These observations obscure the link between GBA variants and PD pathogenesis and point towards a role for unidentified additional genetic and/or environmental risk factors or second hits in GBA-PD. In this study, we explored whether rare genetic variants may be additional risk factors for PD in two families segregating the PD-associated GBA1 variants c.115+1G&gt;A (ClinVar ID: 93445) and p.L444P (ClinVar ID: 4288). Our analysis identified rare genetic variants of the HSP70 co-chaperone DnaJ homolog subfamily B member 6 (DNAJB6) and lysosomal protein prosaposin (PSAP) as additional factors possibly influencing PD risk in the two families. In comparison to the wild-type proteins, variant DNAJB6 and PSAP proteins show altered functions in the context of cellular alpha-synuclein homeostasis when expressed in reporter cells. Furthermore, the segregation pattern of the rare variants in the genes encoding DNAJB6 and PSAP indicated a possible association with PD in the respective families. The occurrence of second hits or additional PD cosegregating rare variants has important implications for genetic counseling in PD families with GBA1 variant carriers and for the selection of PD patients for GBA targeted treatments
The epidemiology, Clinical Manifestations, radiology, microbiology, treatment, and prognosis of echinococcosis: Results of NENEHATUN study
Aim: Echinococcosis, caused by Echinococcus species, is an important zoonotic disease causing major health problems in humans and animals. Herein, we aimed to evaluate the epidemiology, clinical and laboratory parameters, radiological, serological, pathological, and treatment protocols of followed-up cases of hydatidosis.
Methods: A total of 550 patients diagnosed with hydatid cyst disease were included in this study. Patients who were positive for one or more of the enzyme-linked immunosorbent assay or indirect hemagglutination test, pathological results, or radiological findings were examined. The data analyzed were collected from nine centers between 2008 and 2020. Records were examined retrospectively.
Results: Among the patients, 292 (53.1%) were women and 258 (46.9%) were men. The patients' mean age was 44.4 +/- 17.4 years. A history of living in rural areas was recorded in 57.4% of the patients. A total of 435 (79.1%) patients were symptomatic. The most common symptoms were abdominal pain in 277 (50.4%), listlessness in 244 (44.4%), and cough in 140 (25.5%) patients. Hepatomegaly was found in 147 (26.7%), and decreased breath sounds were observed in 124 (22.5%) patients. Radiological examination was performed in all cases and serological methods were also applied to 428 (77.8%) patients. The most frequently applied serological test was IHA (37.8%). A single cyst has been found in 66% patients. Hepatic involvement occurred in 327 (59.4%), pulmonary involvement was found in 128 (23.3%), whereas both of them were recorded in 43 (7.8%) patients. Splenic involvement was only detected in nine (1.6%) patients. Echinococcus granulosus (72.5%) was most frequently detected. Cyst diameters of 56.9% of the patients were in the range of 5-10 cm. A total of 414 (75.2%) patients received albendazole as an antiparasitic. Mortality was noted in nine (1.6%) patients.
Conclusion: Echinococcosis is an important public health problem in Turkey. It can affect the social, economic, and political structures of the community. Public education and awareness are extremely important
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