Comparison of Participation Constraints in Recreational Physical Activity of the Teachers Working Iğdir and Erzurum Provinces

The aim of the study is to compare the participation constraint for leisure activities of teachers who working In the Ministry of National Education (MNE). In order to determine the leisure time constraints leisure constraints scales (LCS) were applied to the participants. A total of 413 teachers (258 male and 155 female) working in the Ministry of National Education participated in the study in Iğdır and Erzurum provinces. No statistically significant difference was found at the level of p <0,05 in any of the sub-dimensions as a result of the inter-city comparison of the female participants. While there was a significant difference in the lack of information, facilities and time sub-dimensions of the male participants in the study, p <0.05 was not significant difference in the other sub-dimensions. It is seen that the difference is in favor of the participants from the province of Erzurum. There was no statistically significant difference between the two cities in terms of lack of information, facilities and time (p <0,05) in the subscales, as in male participants. The female teachers who working Iğdır and Erzurum province there was no difference between the scores in the LCS and the mean scores of both groups were close to each other. For the male participants working in Iğdır province, it was determined that the lack of information, facilities and time constraints were more effective in participation of recreational physical activities than male participants working in Erzurum province

A computational approach to decipher chromatosome structure determinants

In eukaryotic cells, DNA transcription, replication and repair events are controlled by the regulation of DNA compaction mechanisms that determine the open and closed chromatin states. Nucleosomes are the basic DNA packaging units of chromatin. The nucleosome core (NC) consists of a core histone protein octamer with approximately two tight superhelical turns of DNA wrapped around it. The NC is extended at its entry and exit points by linker DNA (L-DNA) and a linker histone (LH) protein binds between the two L-DNA arms to form a chromatosome. The dyad is the single DNA base pair between the nucleosome entry and exit points determining the symmetry axis and is used to define the position of LH binding to a nucleosome. For LH - nucleosome binding, previous studies indicate both on- and off-dyad binding modes, as well as different LH orientations. Thus, the molecular determinants of the structure of LH – nucleosome complex and the dynamics of LH – nucleosome binding are not fully understood. The aim of the research described here was to obtain an atomic-detail level understanding of chromatosome formation. Analysis of the experimentally determined structures of LH – nucleosome complexes showed that instead of a single 3D structure, an ensemble of structures of LH – nucleosome complexes exists. To understand the distribution of these ensembles, normal mode analysis (NMA), standard and accelerated molecular dynamics (MD & AMD) and Brownian dynamics (BD) simulations were applied to LH, nucleosome and chromatosome systems. MD and AMD simulations showed that the globular domain of the LH (LH GD) prefers to be in its closed form in solution. Upon nucleosome binding, the LH GD structure transformed to an open structure due to hydrophobic interactions with the L-DNA of the nucleosome. Additionally, LH GD binding constrained the flexibility of the L-DNA and affected the directions of movement of the L-DNA arms. BD simulations indicated that various chromatosome configurations were possible depending on LH GD sequence and L-DNA opening angles. These findings suggest that LH – nucleosome binding is mediated by a combination of conformational selection and induced fit mechanisms. Further BD simulations show that chromatosome configurations were affected by single point mutations in the LH GD and varied for different LH isoforms. My results indicate that by making specific single point mutation exchanges, it is possible to swap LH – nucleosome configurations among different LH GD isoforms. Similar shifts were observed in chromatosome configuration upon introduction of post translational modifications (PTMs) in the LH GD. I applied BD simulations to compute dissociation rate constant (koff) values and compare them with previously reported fluorescence recovery after photobleaching (FRAP) data on the binding of various LH mutants to chromatin. The results of the BD simulations correspond with the relative trends in measured FRAP recovery half-times (t50) of LH – chromatin binding of various LH mutants. The results thus enable the interpretation of the FRAP data in terms of a physical model of LH – nucleosome binding. My thesis provides detailed insights into the structure, dynamics and kinetics of chromatosome formation in eukaryotes. The results presented in this work can guide further experiments on the sequence determinants of LH – nucleosome binding

Effects of Long Term Volleyball Training on Mineral Metabolism and Hematological Parameters

Objective: Following acute exercises, changes in mineral and hematological concentrations are observed in the organism depending on the exercise, but regular and submaximal exercises do not fully reveal the mineral and hematological effects. This study was conducted to determine the effects of volleyball training on mineral metabolism and hematological parameters over a period of three months. Method:The research group included 20 healthy male volunteers in the 13-16 age group. A training program of 80 minutes per day, 4 days per week, for three months was applied to the athletes who participated in the study. Blood samples were taken twice before and at the end of the training sessions from the resting athletes. Sodium, potassium, chlorine, calcium, magnesium, WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, MPV and PDW levels were determined in the blood samples collected. The data were analyzed using the SPSS 22 package program. The Paired Samples t-test was used to compare the research group's pre-final test data. Significance was considered to be p>0.05. Finding: The analysis showed that the research group had statistically significant differences in sodium, chlorine, phosphorus, magnesium, RBC, HCT, MCV, MCH, MCHC, MPV and PCT levels (p<0.05) and no statistically significant differences in potassium, calcium, WBC, HGB, PLT, RDW-CV and PDW levels (p>0.05). Conclusion: As a result, volleyball training over a long period of time has been observed to cause changes in mineral metabolism and some hematological parameters. It was found that the regular training schedule caused differences in some mineral and whole blood values. In the light of this information, we believe that long-term adolescent training will have a positive impact on the health of athletes

Problems in the participatory approach for the watershed management: an evaluation related to the forestry sector

Bu bildiride, ormancılık sektörüyle doğrudan ilişkili olan havza yönetimi sürecinde katılımcı planlamaya geçiş ve planlamanın etkin bir şekilde yürütülmesinde yaşanan sorunların değerlendirilmesi amaçlanmaktadır. Bu amaçla öncelikle havza yönetimi kavramı ve orman kaynakları yönetimiyle olan ilişkisi üzerinde durulmaktadır. Ardından, Ülkemiz ormancılık sektörünün diğer sektör ve kurumlarla olan etkileşimi; özellikle kırsal kalkınma ve ormancılık faaliyetleri planlama çalışmaları kapsamında ele alınarak irdelenmektedir.In this paper, it is aimed that the factors which are effective on the passing into participatory planning in watershed management process and its implementation effectively are evaluated. For this purpose, firstly watershed management concept and its relations with the management of forest resources will be reviewed and then the interaction of forestry sector with other sectors, rural development and forestry activities will be evaluated in the scope of planning activities

The Role of EREG, PTPN1, and SERPINB7 Genes in the Pathogenesis of Psoriasis: May SERPINB7 Be Protective and a Marker of Severity for Psoriasis?

Introduction: Psoriasis is a chronic inflammatory dermatological disease with complex pathogenesis in which many immune system cells, including keratinocytes, play a role. Many genes regulate the proliferation of keratinocytes and other immune cells that have essential roles in the pathogenesis of psoriasis.The expressions of EREG, PTPN1, and SERPINB7 genes were shown as upregulated in psoriatic skins in a few studies previously. Objectives: We aimed to evaluate the expressions of these genes in psoriatic lesional skin and com-pared them with non-lesional adjacent skin of the same patients and normal skin of healthy controls. Results: Our results revealed that the expressions of EREG and PTPN1 genes were upregulated,whereas the SERPINB7 gene expression was down regulated in the psoriatic skin of the patients than normal skin of controls. Moreover, the expression level of the SERPINB7 gene was also negatively correlated with the severity of the disease among patients. Conclusions: According to our results, overexpression of EREG and PTPN1 genes, and decreased expression of SERPINB7 gene may lead to the development of psoriasis

Putative second hit rare genetic variants in families with seemingly GBA-associated Parkinson's disease

Rare variants in the beta-glucocerebrosidase gene (GBA1) are common genetic risk factors for alpha synucleinopathy, which often manifests clinically as GBA-associated Parkinson's disease (GBA-PD). Clinically, GBA-PD closely mimics idiopathic PD, but it may present at a younger age and often aggregates in families. Most carriers of GBA variants are, however, asymptomatic. Moreover, symptomatic PD patients without GBA variant have been reported in families with seemingly GBA-PD. These observations obscure the link between GBA variants and PD pathogenesis and point towards a role for unidentified additional genetic and/or environmental risk factors or second hits in GBA-PD. In this study, we explored whether rare genetic variants may be additional risk factors for PD in two families segregating the PD-associated GBA1 variants c.115+1G&amp;gt;A (ClinVar ID: 93445) and p.L444P (ClinVar ID: 4288). Our analysis identified rare genetic variants of the HSP70 co-chaperone DnaJ homolog subfamily B member 6 (DNAJB6) and lysosomal protein prosaposin (PSAP) as additional factors possibly influencing PD risk in the two families. In comparison to the wild-type proteins, variant DNAJB6 and PSAP proteins show altered functions in the context of cellular alpha-synuclein homeostasis when expressed in reporter cells. Furthermore, the segregation pattern of the rare variants in the genes encoding DNAJB6 and PSAP indicated a possible association with PD in the respective families. The occurrence of second hits or additional PD cosegregating rare variants has important implications for genetic counseling in PD families with GBA1 variant carriers and for the selection of PD patients for GBA targeted treatments