Parasitbekämpning och renarnas kondition

Av de parasitmedel som man anvandt hade Ivomec den basta effekten på hudkorm (Oedemagena tarandi). Hos renar som behandlats med denna medicin kunde man observera varken larvar av hudkorm eller svalgkorm (Cephenemyia trompe)

Biomarkers and fluctuating asymmetry as indicators of pollution-induced stress in two hole-nesting passerines.

Summary 1. The stress caused by air pollutants was studied at biochemical, morphological and ecological levels in the Pied Flycatcher, Ficedula hypoleuca , and Great Tit, Parus major , nestlings in 10 study sites along the pollution gradient of a copper smelter. 2. First, stress was measured using four biomarkers from blood and liver: ethoxyresorufin O -deethylase (EROD) enzyme, haemoglobin, stress protein Hsp70 and deltaaminolevulinic acid dehydratase (ALA-d) enzyme. Second, the amount of fluctuating asymmetry (FA) in the length of the 3rd primary and the outermost rectrix (in P. major ) and the length and thickness of the tarsus were measured. These stress indicators were further examined in relation to the breeding performance of birds, i.e. the ecological response. 3. EROD activity was increased in F. hypoleuca nestlings near the pollution source. In P. major , it was not related to the pollution gradient but correlated well with the proportion of starved nestlings, i.e. the nutritional stress during the nestling period. The variations in haemoglobin, Hsp70 and ALA-d enzyme activity were not significantly related to pollution gradient. 4. The tarsus length of F. hypoleuca nestlings and primary length of P. major nestlings showed increased asymmetry in the vicinity of the pollution source. Breeding success decreased towards the pollution source in both species. 5. In F. hypoleuca , the pollution-related stress was verified at all three target levels. In P. major , pollution-related effects were found at morphological and ecological levels but not at the biochemical level, which suggests that direct toxic effect of heavy metals was not the main mechanism in this species

Integration of Solexa sequences on an ultradense genetic map in Brassica rapa L.

<p>Abstract</p> <p>Background</p> <p>Sequence related amplified polymorphism (SRAP) is commonly used to construct high density genetic maps, map genes and QTL of important agronomic traits in crops and perform genetic diversity analysis without knowing sequence information. To combine next generation sequencing technology with SRAP, Illumina's Solexa sequencing was used to sequence tagged SRAP PCR products.</p> <p>Results</p> <p>Three sets of SRAP primers and three sets of tagging primers were used in 77,568 SRAP PCR reactions and the same number of tagging PCR reactions respectively to produce a pooled sample for Illumina's Solexa sequencing. After sequencing, 1.28 GB of sequence with over 13 million paired-end sequences was obtained and used to match Solexa sequences with their corresponding SRAP markers and to integrate Solexa sequences on an ultradense genetic map. The ultradense genetic bin map with 465 bins was constructed using a recombinant inbred (RI) line mapping population in <it>B. rapa</it>. For this ultradense genetic bin map, 9,177 SRAP markers, 1,737 integrated unique Solexa paired-end sequences and 46 SSR markers representing 10,960 independent genetic loci were assembled and 141 unique Solexa paired-end sequences were matched with their corresponding SRAP markers. The genetic map in <it>B. rapa </it>was aligned with the previous ultradense genetic map in <it>B. napus </it>through common SRAP markers in these two species. Additionally, SSR markers were used to perform alignment of the current genetic map with other five genetic maps in <it>B. rapa </it>and <it>B. napus</it>.</p> <p>Conclusion</p> <p>We used SRAP to construct an ultradense genetic map with 10,960 independent genetic loci in <it>B. rapa </it>that is the most saturated genetic map ever constructed in this species. Using next generation sequencing, we integrated 1,878 Solexa sequences on the genetic map. These integrated sequences will be used to assemble the scaffolds in the <it>B. rapa </it>genome. Additionally, this genetic map may be used for gene cloning and marker development in <it>B. rapa </it>and <it>B. napus</it>.</p

OSBPL10, a novel candidate gene for high triglyceride trait in dyslipidemic Finnish subjects, regulates cellular lipid metabolism

Analysis of variants in three genes encoding oxysterol-binding protein (OSBP) homologues (OSBPL2, OSBPL9, OSBPL10) in Finnish families with familial low high-density lipoprotein (HDL) levels (N = 426) or familial combined hyperlipidemia (N = 684) revealed suggestive linkage of OSBPL10 single-nucleotide polymorphisms (SNPs) with extreme end high triglyceride (TG; >90th percentile) trait. Prompted by this initial finding, we carried out association analysis in a metabolic syndrome subcohort (Genmets) of Health2000 examination survey (N = 2,138), revealing association of multiple OSBPL10 SNPs with high serum TG levels (>95th percentile). To investigate whether OSBPL10 could be the gene underlying the observed linkage and association, we carried out functional experiments in the human hepatoma cell line Huh7. Silencing of OSBPL10 increased the incorporation of [3H]acetate into cholesterol and both [3H]acetate and [3H]oleate into triglycerides and enhanced the accumulation of secreted apolipoprotein B100 in growth medium, suggesting that the encoded protein ORP10 suppresses hepatic lipogenesis and very-low-density lipoprotein production. ORP10 was shown to associate dynamically with microtubules, consistent with its involvement in intracellular transport or organelle positioning. The data introduces OSBPL10 as a gene whose variation may contribute to high triglyceride levels in dyslipidemic Finnish subjects and provides evidence for ORP10 as a regulator of cellular lipid metabolism

Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes.

Fourier transform infrared (FTIR) microspectroscopy and confocal imaging have been used to demonstrate that the neutral rhenium(i) tricarbonyl 1,10-phenanthroline complex bound to 4-cyanophenyltetrazolate as the ancillary ligand is able to localise in regions with high concentrations of polar lipids such as phosphatidylethanolamine (PE), sphingomyelin, sphingosphine and lysophosphatidic acid (LPA) in mammalian adipocytes

Infångande, hantering och transport av renar med hjälp av Domedesan R preparat

Tama renar och vilda skogsrenar var låtta att hantera ungefar 6 minuter efter Domosedaninjectionen och preparatet inverkade i medeltal 2,5 timmars tid, varierande från 30 minuter till 4 timmar beroande på dosens storlek