Sphingomyelin metabolism is involved in the differentiation of MDCK cells induced by environmental hypertonicity

Sphingolipids (SLs) are relevant lipid components of eukaryotic cells. Besides regulating various cellular processes, SLs provide the structural framework for plasma membrane organization. Particularly, SM is associated with detergent-resistant microdomains. We have previously shown that the adherens junction (AJ) complex, the relevant cell-cell adhesion structure involved in cell differentiation and tissue organization, is located in an SM-rich membrane lipid domain. We have also demonstrated that under hypertonic conditions, Madin-Darby canine kidney (MDCK) cells acquire a differentiated phenotype with changes in SL metabolism. For these reasons, we decided to evaluate whether SM metabolism is involved in the acquisition of the differentiated phenotype of MDCK cells. We found that SM synthesis mediated by SM synthase 1 is involved in hypertonicity-induced formation of mature AJs, necessary for correct epithelial cell differentiation. Inhibition of SM synthesis impaired the acquisition of mature AJs, evoking a disintegration-like process reflected by the dissipation of E-cadherin and β- and α-catenins from the AJ complex. As a consequence, MDCK cells did not develop the hypertonicity-induced differentiated epithelial cell phenotype.Fil: Favale, Nicolas Octavio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Santacreu, Bruno Jaime. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Pescio, Lucila Gisele. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Marquez, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Rioja. Departamento de Ciencias de la Salud y Educación; ArgentinaFil: Sterin, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentin

The European Commission's public consultation on the review of EU copyright rules: a response by the CREATe Centre

No abstract available

On the Xiao conjecture for plane curves

Let $f: S\longrightarrow B$ be a non-trivial fibration from a complex projective smooth surface $S$ to a smooth curve $B$ of genus $b$. Let $c_f$ the Clifford index of the generic fibre $F$ of $f$. In [arXiv:1401.7502v4] it is proved that the relative irregularity of $f$, $q_f=h^{1,0}(S)-b$ is less than or equal to $g(F)-c_f$. In particular this proves the (modified) Xiao's conjecture: $q_f\le 1+g(F)/2$ for fibrations of general Clifford index. In this short note we assume that the generic fiber of $f$ is a plane curve of degree $d\ge 5$ and we prove that $q_f\le g(F)-c_f-1$. In particular we obtain the conjecture for families of quintic plane curves. This theorem is implied for the following result on infinitesimal deformations: let $F$ a smooth plane curve of degree $d\ge 5$ and let $\xi$ be an infinitesimal deformation of $F$ preserving the planarity of the curve. Then the rank of the cup-product map $\cdot \xi: H^0(F,\omega_F) \rightarrow H^1(F,O_F)$ is at least $d-3$. We also show that this bound is sharp.Comment: 8 pages. Some typos have been corrected. To appear in "Geometriae Dedicata

Calabi-Yau quotients with terminal singularities

In this paper we are interested in quotients of Calabi-Yau threefolds with isolated singularities. In particular, we analyze the case when $X/G$ has terminal singularities. We prove that, if $G$ is cyclic of prime order and $X/G$ has terminal singularities, then $G$ has order $2,3$ or $5$.Comment: 15 pages, no figures. Current version has some changes in the structure of the article, some corrected typos and an up-to-date bibliograph

New examples of Calabi-Yau threefolds and genus zero surfaces

We classify the subgroups of the automorphism group of the product of 4 projective lines admitting an invariant anticanonical smooth divisor on which the action is free. As a first application, we describe new examples of Calabi-Yau 3-folds with small Hodge numbers. In particular, the Picard number is 1 and the number of moduli is 5. Furthermore, the fundamental group is non-trivial. We also construct a new family of minimal surfaces of general type with geometric genus zero, K^2=3 and fundamental group of order 16. We show that this family dominates an irreducible component of dimension 4 of the moduli space of the surfaces of general type.Comment: 18 pages; v2: simplified some arguments in the last section, final version to appear on Communications in Contemporary Mathematic

Resolución alternativa de conflitos entre cultura, normas y tutela del consumidor en Europa

Il contributo offre alcune riflessioni sugli strumenti alternativi di soluzione dei conflitti, la loro efficacia e la loro adeguatezza. Ci si chiede se l'autonomia contrattuale delle parti garantisca la realizzazione degli interessi perseguiti dagli interessati anche sul versante delle composizioni delle liti. Allo stesso tempo, vengono evidenziati alcuni punti deboli di siffatti strumenti soprattutto in relazione alle strade giurisdizionali