The chemopreventive polyphenol Curcumin prevents hematogenous breast cancer metastases in immunodeficient mice

Dissemination of metastatic cells probably occurs long before diagnosis of the primary tumor. Metastasis during early phases of carcinogenesis in high risk patients is therefore a potential prevention target. The plant polyphenol Curcumin has been proposed for dietary prevention of cancer. We therefore examined its effects on the human breast cancer cell line MDA-MB-231 in vitro and in a mouse metastasis model. Curcumin strongly induces apoptosis in MDA- MB- 231 cells in correlation with reduced activation of the survival pathway NF kappa B, as a consequence of diminished I kappa B and p65 phosphorylation. Curcumin also reduces the expression of major matrix metalloproteinases (MMPs) due to reduced NF kappa B activity and transcriptional downregulation of AP-1. NF kappa B/p65 silencing is sufficient to downregulate c-jun and MMP expression. Reduced NF kappa B/AP-1 activity and MMP expression lead to diminished invasion through a reconstituted basement membrane and to a significantly lower number of lung metastases in immunodeficient mice after intercardiac injection of 231 cells (p=0.0035). 68% of Curcumin treated but only 17% of untreated animals showed no or very few lung metastases, most likely as a consequence of down-regulation of NF kappa B/AP-1 dependent MMP expression and direct apoptotic effects on circulating tumor cells but not on established metastases. Dietary chemoprevention of metastases appears therefore feasible. Copyright (c) 2007 S. Karger AG, Basel

CIXL2: A Crossover Operator for Evolutionary Algorithms Based on Population Features

In this paper we propose a crossover operator for evolutionary algorithms with real values that is based on the statistical theory of population distributions. The operator is based on the theoretical distribution of the values of the genes of the best individuals in the population. The proposed operator takes into account the localization and dispersion features of the best individuals of the population with the objective that these features would be inherited by the offspring. Our aim is the optimization of the balance between exploration and exploitation in the search process. In order to test the efficiency and robustness of this crossover, we have used a set of functions to be optimized with regard to different criteria, such as, multimodality, separability, regularity and epistasis. With this set of functions we can extract conclusions in function of the problem at hand. We analyze the results using ANOVA and multiple comparison statistical tests. As an example of how our crossover can be used to solve artificial intelligence problems, we have applied the proposed model to the problem of obtaining the weight of each network in a ensemble of neural networks. The results obtained are above the performance of standard methods

A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI).

This work was supported by: Associazione Italiana Ricerca sul Cancro (AIRC) [Grant 5 x mille n.9980, (to M.F., F.M. and A. N.)]; AIRC I.G. [n. 14,326 (to M.F.)], [n.10136 and 16,722 (A.N.)], [n.15426 (to F.F.)]. AIRC and Fondazione CaRiCal co-financed Multi Unit Regional Grant 2014 [n.16695 (to F.M.)]. Italian Ministry of Health 5 × 1000 funds (to F.F). A.G R. was supported by Associazione Italiana contro le Leucemie-Linfomi-Mielomi (AIL) Cosenza - Fondazione Amelia Scorza (FAS). S.M. C.M., F.V., L. E., S. B., were supported by AIRC.Peer reviewedPostprin

Specificity Between Lactobacilli And Hymenopteran Hosts Is The Exception Rather Than The Rule

Lactobacilli (Lactobacillales: Lactobacillaceae) are well known for their roles in food fermentation, as probiotics, and in human health, but they can also be dominant members of the microbiota of some species of Hymenoptera (ants, bees, and wasps). Honey bees and bumble bees associate with host-specific lactobacilli, and some evidence suggests that these lactobacilli are important for bee health. Social transmission helps maintain associations between these bees and their respective microbiota. To determine whether lactobacilli associated with social hymenopteran hosts are generally host specific, we gathered publicly available Lactobacillus 16S rRNA gene sequences, along with Lactobacillus sequences from 454 pyrosequencing surveys of six other hymenopteran species (three sweat bees and three ants). We determined the comparative secondary structural models of 16S rRNA, which allowed us to accurately align the entire 16S rRNA gene, including fast-evolving regions. BLAST searches and maximum-likelihood phylogenetic reconstructions confirmed that honey and bumble bees have host-specific Lactobacillus associates. Regardless of colony size or within-colony oral sharing of food (trophallaxis), sweat bees and ants associate with lactobacilli that are closely related to those found in vertebrate hosts or in diverse environments. Why honey and bumble bees associate with host-specific lactobacilli while other social Hymenoptera do not remains an open question. Lactobacilli are known to inhibit the growth of other microbes and can be beneficial whether they are coevolved with their host or are recruited by the host from environmental sources through mechanisms of partner choice.National Science Foundation PRFB-1003133, DEB-0919519Texas Higher Education Coordinating Board 01923, National Institutes of Health GM067317Integrative BiologyCellular and Molecular BiologyCenter for Computational Biology and BioinformaticsBrackenridge Field Laborator

The prevalence of trunk asymmetries in the small island state of Malta

Background: Malta, the smallest member state of the European Union is constituted of two inhabited islands Malta and Gozo. In the Maltese islands there has not been any large population size definitive study concerning the incidence of trunk asymmetries that may indicate Adolescent Idiopathic Scoliosis (AIS) amongst the general population. Scoliosis is one of the most deforming orthopaedic conditions confronting children. To confirm the orthopaedic condition of scoliosis one has to carry out a visual examination that usually consists of the Adam’s Forward Bend test, this is followed by the measurement of trunk rotation with the use of a Bunnell Scoliometer. Should the angle of trunk rotation be more than five degrees then the positively screened student be referred for x-Rays and a 10 degree Cobb angle taken as being required to confirm the diagnosis of Scoliosis. The lack of a full scale study together with the apparent lack of awareness regarding the condition has prompted the authors to research the situation on all Gozitan children aged between 13 and 15 years of age. It was decided to measure Trunk Asymmetry and the aim of the study was to obtain statistical data on the occurrence of trunk asymmetries amongst the Gozitan population, to further analyse the ratio of distribution of trunk asymmetries between female and male students and finally to refer the positively screened students to the relevant medical authorities for x-ray to confirm a scoliosis diagnosis. Methods: This quantitative study design was carried out on all children aged between 13 to 15 years old over a five-month period. An Adam’s forward bend test and Scoliometer reading were taken for each participant consenting to this study. To minimse bias a qualified full time Physiotherapist graduated with a Bachelor of Science Honours degree in Physiotherapy since 2012, carried out these tests in the selected schools. Results: The results of the study concluded that 5.3% of the adolescent population in Gozo suffer from trunk asymmetries (13 out of 245). Prevalence of trunk asymmetry was calculated using the 95% confidence interval and the Chi square tests had a significant p-value. Further analysis showed that 69% of these were female and 31% were male. These results demonstrate that the prevalence of trunk asymmetries in Gozitan adolescents is comparable to that stated within the current literature. Conclusions: Results from the study confirms that trunk asymmetry is relatively common within the Maltese population. This might be indicative that a significant portion of the Maltese adolescent population might suffer from Adolescent Idiopathic Scoliosis. The intention of this research is to increase the general public’s awareness of the condition AIS, to make this condition more prominent to members of the allied professions, to reinforce the need for school screening projects and finally to ensure that the condition Trunk Asymmetry and Scoliosis is given the importance that it requires in the curriculum of study for physiotherapists.peer-reviewe

Re veri e fasulli zar. Intesa ed antagonismo polacco moscovita nella historia di moscovia dell'abate pistoiese Alessandro Cilli

Review question/objectives: The objective of this review is to synthesize the evidence of registered nurses’ and midwives’ experiences of videoconferencing in clinical and professional practice. For the purpose of this review, professional practice is defined as activities including education and training, maintaining competencies, networking and peer support. Clinical practice includes any activities directly related to patient/client care. The primary questions to be answered are: What are the the perceptions and experiences of nurses and midwives in relation to the appropriateness and meaningfulness of videoconferencing; how do they perceive its use in professional and clinical practice? A secondary question to be addressed in this review is: What are nurse and midwife accounts of the factors that influence the use of videoconferencing in professional and clinical practice? Center conducting the review: Centre for Evidence-based Healthy Ageing – an Affiliate Center of the Joanna Briggs Institute; Queensland University of Technology, Brisbane, Queensland

Spatial Training and Calculus Ability: Investigating Impacts on Student Performance and Cognitive Style

Undergraduate calculus is a foundational mathematics sequence that previews the sophistication students will need to succeed in higher-level courses. However, students often struggle with concepts in calculus because they are more abstract and visual than those in other foundational mathematics courses. Additionally, women continue to be underrepresented in the STEM fields. This study builds on previous work indicating a malleability in spatial ability by testing whether improvement occurs in students’ spatial and mathematics ability after implementing spatial training in calculus courses. The researchers also measured associations between spatial training and self-reported cognitive style. While spatial training did not significantly improve calculus and spatial skills, the researchers measured impacts on the psychological correlate of cognitive learning style. This is important as non-spatial cognitive preferences can result in students not recognizing the dynamic information encapsulated in graphical images, which is a large component of calculus. These results may have practical implications for teaching calculus at the undergraduate level and may, with further research, help to narrow the gender gap in the STEM fields