This work was supported by: Associazione Italiana Ricerca sul Cancro (AIRC) [Grant 5 x mille n.9980, (to M.F., F.M. and A. N.)]; AIRC I.G. [n. 14,326 (to M.F.)], [n.10136 and 16,722 (A.N.)], [n.15426 (to F.F.)]. AIRC and Fondazione CaRiCal co-financed Multi Unit Regional Grant 2014 [n.16695 (to F.M.)]. Italian Ministry of Health 5 × 1000 funds (to F.F). A.G R. was supported by Associazione Italiana contro le Leucemie-Linfomi-Mielomi (AIL) Cosenza - Fondazione Amelia Scorza (FAS). S.M. C.M., F.V., L. E., S. B., were supported by AIRC.Peer reviewedPostprin

Baio, Gabriella

Basso, Luca

Boccardo, Simona

Calabrese, Massimo

Castronovo, Angela

Cilli, Michele

Cutrona, Giovanna

Emionite, Laura

Fais, Franco

Ferrarini, Manlio

Gentile, Massimo

Massucco, Carlotta

Matis, Serena

Morabito, Fortunato

Neri, Antonino

Neumaier, Carlo Emanuele

Pace, Daniele

Ravina, Marco

Recchia, Anna Grazia

Rosa, Francesca

Salvi, Sandra

Truini, Mauro

Valdora, Francesca

Clinical Immunology

English

Nerih, Antonino

Research Archive

A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI).

Chronic lymphocytic leukemia (CLL) is the most prevalent leukemia among adults. Despite its indolent nature, CLL remains an incurable disease. Herein we aimed to monitor CLL disease engraftment and,progression/regression in a xenograft CLL mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). Spleen contrast enhancement, quantified as percentage change in signal intensity upon USPIO administration, demonstrated a difference due to a reduced USPIO uptake, in the spleens of mice injected with CLL cells (NSG-CLL, n=71) compared to controls (NSG-CTR, n=17). These differences were statistically significant both after 2 and 4weeks from CLL cells injection. In addition comparison of mice treated with rituximab with untreated controls for changes in spleen iron uptake confirmed that it is possible to monitor treatment efficacy in this mouse model of CLL using USPIO-enhanced MRI. Further applications could include the preclinical in vivo monitoring of new therapies and the clinical evaluation of CLL patients

F. Valdora

G. Cutrona

S. Matis

F. Morabito

C. Massucco

L. Emionite

S. Boccardo

L. Basso

A.G. Recchia

S. Salvi

F. Rosa

M. Gentile

M. Ravina

D. Pace

A. Castronovo

M. Cilli

M. Truini

M. Calabrese

A. Neri

C.E. Neumaier

F. Fais

G. Baio

M. Ferrarini

AIR Universita degli studi di Milano

A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI)

Chronic lymphocytic leukemia (CLL) is the most prevalent leukemia among adults. Despite its indolent nature, CLL remains an incurable disease. Herein we aimed to monitor CLL disease engraftment and, progression/regression in a xenograft CLL mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI). Spleen contrast enhancement, quantified as percentage change in signal intensity upon USPIO administration, demonstrated a difference due to a reduced USPIO uptake, in the spleens of mice injected with CLL cells (NSG-CLL, n = 71) compared to controls (NSG-CTR, n = 17). These differences were statistically significant both after 2 and 4 weeks from CLL cells injection. In addition comparison of mice treated with rituximab with untreated controls for changes in spleen iron uptake confirmed that it is possible to monitor treatment efficacy in this mouse model of CLL using USPIO-enhanced MRI. Further applications could include the preclinical in vivo monitoring of new therapies and the clinical evaluation of CLL patients

VALDORA, FRANCESCA

CUTRONA, GIOVANNA

EMIONITE, LAURA

TRUINI, MAURO

FAIS, FRANCO

FERRARINI, MANLIO

Archivio istituzionale della ricerca - Università di Genova

https://air.unimi.it/retrieve/handle/2434/423553/656912/Valdora_Clin%20Immunol_2016_manuscript.pdf

A non-invasive approach to monitor chronic lymphocytic leukemia engraftment in a xenograft mouse model using ultra-small superparamagnetic iron oxide-magnetic resonance imaging (USPIO-MRI).

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AIR Universita degli studi di Milano

Archivio istituzionale della ricerca - Università di Genova