Estimation of magnitudes of debris flows in selected torrential watersheds in Slovenia

In this paper the application of different methods for estimation of magnitudes of rainfall-induced debris flows in 18 torrents in the Upper Sava River valley, NW Slovenia, and in 2 torrents in Pohorje, N Slovenia is described. Additional verification of the methods was performed in the torrential watersheds with active debris flows in the recent past (Predelica and Brusnik in the Soca River basin, W Slovenia). For some of the methods, the knowledge of morphometric characteristics of a torrential watershed, torrential channel and torrential fan is enough. For other methods, a mathematical tool (HEC-HMS) had to be applied in order to develop a hydrologic run-off model of precipitation that can trigger debris flows. Computed debris-flow magnitudes were of the order between 6,500 m(3) and 340,000 m(3). Their values are a function of torrential watershed parameters, such as: watershed area, Melton number, fan gradient, and torrential channel gradient. The investigated fans were classified into 3 groups with regard to the debris-flow hazard: debris-flow fans (hazard exists), torrential fans (no hazard), and transitional fans (debris flows are possible, but with low possibility). A limit between debris-flow fans and torrential fans is proposed: Melton number 0.3 and torrential fan gradient 4 degrees, that is, 7%. Out of 24 investigated torrential fans, 13 fans were classified into the group of debris-flow fans, 5 fans were classified into the group of torrential fans, and the rest 6 fans were classified into the group of transitional fans

Connexin-mediated signaling in nonsensory cells is crucial for the development of sensory inner hair cells in the mouse cochlea

open9siMutations in the genes encoding for gap junction proteins connexin 26 (Cx26) and connexin 30 (Cx30) have been linked to syndromic and nonsyndromic hearing loss in mice and humans. The release of ATP from connexin hemichannels in cochlear nonsensory cells has been proposed to be the main trigger for action potential activity in immature sensory inner hair cells (IHCs), which is crucial for the refinement of the developing auditory circuitry. Using connexin knock-out mice, we show that IHCs fire spontaneous action potentials even in the absence of ATP-dependent intercellular Ca 2 signaling in the nonsensory cells. However, this signaling from nonsensory cells was able to increase the intrinsic IHC firing frequency. We also found that connexin expression is key to IHC functional maturation. In Cx26 conditional knock-out mice (Cx26Sox10-Cre), the maturation of IHCs, which normally occurs at approximately postnatal day 12, was partially prevented. Although Cx30 has been shown not to be required for hearing in young adult mice, IHCs from Cx30 knock-out mice exhibited a comprehensive brake in their development, such that their basolateral membrane currents and synaptic machinery retain a prehearing phenotype. We propose that IHC functional differentiation into mature sensory receptors is initiated in the prehearing cochlea provided that the expression of either connexin reaches a threshold level. As such, connexins regulate one of the most crucial functional refinements in the mammalian cochlea, the disruption of which contributes to the deafness phenotype observed in mice and DFNB1 patients.SIGNIFICANCE STATEMENT The correct development and function of the mammalian cochlea relies not only on the sensory hair cells, but also on the surrounding nonsensory cells. Although the nonsensory cells have been largely implicated in the general homeostasis in the mature cochlea, their involvement in the initial functional differentiation of the sensory inner hair cells is less clear. Using mutant mouse models for the most common form of congenital deafness in humans, which are knock-outs for the gap-junction channels connexin 26 and connexin 30 genes, we show that defects in nonsensory cells prevented the functional maturation of inner hair cells. In connexin knock-outs, inner hair cells remained stuck at a prehearing stage of development and, as such, are unable to process sound information.openJohnson, Stuart L.; Ceriani, Federico; Houston, Oliver; Polishchuk, Roman; Polishchuk, Elena; Crispino, Giulia; Zorzi, Veronica; Mammano, Fabio; Marcotti, WalterJohnson, Stuart L.; Ceriani, Federico; Houston, Oliver; Polishchuk, Roman; Polishchuk, Elena; Crispino, Giulia; Zorzi, Veronica; Mammano, Fabio; Marcotti, Walte

Hair cell maturation is differentially regulated along the tonotopic axis of the mammalian cochlea

Sound amplification within the mammalian cochlea depends upon specialized hair cells, the outer hair cells (OHCs), which possess both sensory and motile capabilities. In various altricial rodents, OHCs become functionally competent from around postnatal day 7 (P7), before the primary sensory inner hair cells (IHCs), which become competent at about the onset of hearing (P12). The mechanisms responsible for the maturation of OHCs and their synaptic specialization remain poorly understood. We report that spontaneous Ca2+ activity in the immature cochlea, which is generated by CaV1.3 Ca2+ channels, differentially regulates the maturation of hair cells along the cochlea. Under near‐physiological recording conditions we found that, similar to IHCs, immature OHCs elicited spontaneous Ca2+ action potentials (APs), but only during the first few postnatal days. Genetic ablation of these APs in vivo, using CaV1.3−/− mice, prevented the normal developmental acquisition of mature‐like basolateral membrane currents in low‐frequency (apical) hair cells, such as IK,n (carried by KCNQ4 channels), ISK2 and IACh (α9α10nAChRs) in OHCs and IK,n and IK,f (BK channels) in IHCs. Electromotility and prestin expression in OHCs were normal in CaV1.3−/− mice. The maturation of high‐frequency (basal) hair cells was also affected in CaV1.3−/− mice, but to a much lesser extent than apical cells. However, a characteristic feature in CaV1.3−/− mice was the reduced hair cell size irrespective of their cochlear location. We conclude that the development of low‐ and high‐frequency hair cells is differentially regulated during development, with apical cells being more strongly dependent on experience‐independent Ca2+ APs

A dataflow platform for applications based on Linked Data

Modern software applications increasingly benefit from accessing the multifarious and heterogeneous Web of Data, thanks to the use of web APIs and Linked Data principles. In previous work, the authors proposed a platform to develop applications consuming Linked Data in a declarative and modular way. This paper describes in detail the functional language the platform gives access to, which is based on SPARQL (the standard query language for Linked Data) and on the dataflow paradigm. The language features interactive and meta-programming capabilities so that complex modules/applications can be developed. By adopting a declarative style, it favours the development of modules that can be reused in various specific execution context

Going to the churches of the Evangelio: Children's Perspectives on Religion in an Indigenous Urban Setting in Buenos Aires

The aim of this article is to reconstruct child perspectives on religion and to analyze them in the context of their participation in social practices (such as family transmission, peer and sibling relationships, and practices within the evangelical community´s churches and a catholic school). For my research, I articulate an ethnographic approach using other methods and fieldwork techniques (usually used in anthropology of childhood, as are certain psychological approaches to children´s points of view), that became fundamental strategies for approaching child voices and practices. In doing so, issues of method and epistemology were contemplated, seeking for ways to critically analyze the link between religious and ethnic identifications. By considering the ways children articulate their own perspectives on the social processes they are involved in, I will specially focus on how Toba children constitute the meaning of the Evangelio (Gospel) religious movement over time.Fil: Garcia Palacios, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

SPARQL Playground: A block programming tool to experiment with SPARQL

SPARQL is a powerful query language for SemanticWeb data sources but one which is quite complex to master. As the block programming paradigm has been succesfully used to teach programming skills, we propose a tool that allows users to build and run SPARQL queries on an endpoint without previous knowledge of the syntax of SPARQL and the model of the data in the endpoint (vocabularies and semantics). This user interface attempts to close the gap between tools for the lay user that do not allow to express complex queries and overtly complex technical tools

Striking circadian neuron diversity and cycling of Drosophila alternative splicing.

Although alternative pre-mRNA splicing (AS) significantly diversifies the neuronal proteome, the extent of AS is still unknown due in part to the large number of diverse cell types in the brain. To address this complexity issue, we used an annotation-free computational method to analyze and compare the AS profiles between small specific groups of Drosophila circadian neurons. The method, the Junction Usage Model (JUM), allows the comprehensive profiling of both known and novel AS events from specific RNA-seq libraries. The results show that many diverse and novel pre-mRNA isoforms are preferentially expressed in one class of clock neuron and also absent from the more standard Drosophila head RNA preparation. These AS events are enriched in potassium channels important for neuronal firing, and there are also cycling isoforms with no detectable underlying transcriptional oscillations. The results suggest massive AS regulation in the brain that is also likely important for circadian regulation

Safety-related Tasks within the Set-Based Task-Priority Inverse Kinematics Framework

In this paper we present a framework that allows the motion control of a robotic arm automatically handling different kinds of safety-related tasks. The developed controller is based on a Task-Priority Inverse Kinematics algorithm that allows the manipulator's motion while respecting constraints defined either in the joint or in the operational space in the form of equality-based or set-based tasks. This gives the possibility to define, among the others, tasks as joint-limits, obstacle avoidance or limiting the workspace in the operational space. Additionally, an algorithm for the real-time computation of the minimum distance between the manipulator and other objects in the environment using depth measurements has been implemented, effectively allowing obstacle avoidance tasks. Experiments with a Jaco$^2$ manipulator, operating in an environment where an RGB-D sensor is used for the obstacles detection, show the effectiveness of the developed system