Accurate detection of dysmorphic nuclei using dynamic programming and supervised classification

A vast array of pathologies is typified by the presence of nuclei with an abnormal morphology. Dysmorphic nuclear phenotypes feature dramatic size changes or foldings, but also entail much subtler deviations such as nuclear protrusions called blebs. Due to their unpredictable size, shape and intensity, dysmorphic nuclei are often not accurately detected in standard image analysis routines. To enable accurate detection of dysmorphic nuclei in confocal and widefield fluorescence microscopy images, we have developed an automated segmentation algorithm, called Blebbed Nuclei Detector (BleND), which relies on two-pass thresholding for initial nuclear contour detection, and an optimal path finding algorithm, based on dynamic programming, for refining these contours. Using a robust error metric, we show that our method matches manual segmentation in terms of precision and outperforms state-of-the-art nuclear segmentation methods. Its high performance allowed for building and integrating a robust classifier that recognizes dysmorphic nuclei with an accuracy above 95%. The combined segmentation-classification routine is bound to facilitate nucleus-based diagnostics and enable real-time recognition of dysmorphic nuclei in intelligent microscopy workflows

Comparison of Support Vector Machine and Back Propagation Neural Network in Evaluating the Enterprise Financial Distress

Recently, applying the novel data mining techniques for evaluating enterprise financial distress has received much research alternation. Support Vector Machine (SVM) and back propagation neural (BPN) network has been applied successfully in many areas with excellent generalization results, such as rule extraction, classification and evaluation. In this paper, a model based on SVM with Gaussian RBF kernel is proposed here for enterprise financial distress evaluation. BPN network is considered one of the simplest and are most general methods used for supervised training of multilayered neural network. The comparative results show that through the difference between the performance measures is marginal; SVM gives higher precision and lower error rates.Comment: 13 pages, 1 figur

Vision-based neural network classifiers and their applications

A thesis submitted for the degree of Doctor of Philosophy of University of LutonVisual inspection of defects is an important part of quality assurance in many fields of production. It plays a very useful role in industrial applications in order to relieve human inspectors and improve the inspection accuracy and hence increasing productivity. Research has previously been done in defect classification of wood veneers using techniques such as neural networks, and a certain degree of success has been achieved. However, to improve results in tenus of both classification accuracy and running time are necessary if the techniques are to be widely adopted in industry, which has motivated this research. This research presents a method using rough sets based neural network with fuzzy input (RNNFI). Variable precision rough set (VPRS) method is proposed to remove redundant features utilising the characteristics of VPRS for data analysis and processing. The reduced data is fuzzified to represent the feature data in a more suitable foml for input to an improved BP neural network classifier. The improved BP neural network classifier is improved in three aspects: additional momentum, self-adaptive learning rates and dynamic error segmenting. Finally, to further consummate the classifier, a uniform design CUD) approach is introduced to optimise the key parameters because UD can generate a minimal set of uniform and representative design points scattered within the experiment domain. Optimal factor settings are achieved using a response surface (RSM) model and the nonlinear quadratic programming algorithm (NLPQL). Experiments have shown that the hybrid method is capable of classifying the defects of wood veneers with a fast convergence speed and high classification accuracy, comparing with other methods such as a neural network with fuzzy input and a rough sets based neural network. The research has demonstrated a methodology for visual inspection of defects, especially for situations where there is a large amount of data and a fast running speed is required. It is expected that this method can be applied to automatic visual inspection for production lines of other products such as ceramic tiles and strip steel

Multiorder polygonal approximation of digital curves

In this paper, we propose a quick threshold-free algorithm, which computes the angular shape of a 2D object from the points of its contour. For that, we have extended the method defined in [4, 5] to a multiorder analysis. It is based on the arithmetical definition of discrete lines [11] with variable thickness. We provide a framework to analyse a digital curve at different levels of thickness. The extremities of a segment provided at a high resolution are tracked at lower resolution in order to refine their location. The method is thresholdfree and automatically provides a partitioning of a digital curve into its meaningful parts