Design automation in synthetic biology : a dual evolutionary strategy

PhD ThesisSynthetic biology o ers a new horizon in designing complex systems. However, unprecedented complexity hinders the development of biological systems to its full potential. Mitigating complexity via adopting design principles from engineering and computer science elds has resulted in some success. For example, modularisation to foster reuse of design elements, and using computer assisted design tools have helped contain complexity to an extent. Nevertheless, these design practices are still limited, due to their heavy dependence on rational decision making by human designers. The issue with rational design approaches here arises from the challenging nature of dealing with highly complex biological systems of which we currently do not have complete understanding. Systematic processes that can algorithmically nd design solutions would be better able to cope with uncertainties posed by high levels of design complexity. A new framework for enabling design automation in synthetic biology was investigated. The framework works by projecting design problems into search problems, and by searching for design solutions based on the dual-evolutionary approach to combine the respective power of design domains in vivo and in silico. Proof-of-concept ideas, software, and hardware were developed to exemplify key technologies necessary in realising the dual evolutionary approach. Some of the areas investigated as part of this research included single-cell-level micro uidics, programmatic data collection, processing and analysis, molecular devices supporting solution search in vivo, and mathematical modelling. These somewhat eclectic collection of research themes were shown to work together to provide necessary means with which to design and characterise biological systems in a systematic fashion

Knowledge Capturing in Design Briefing Process for Requirement Elicitation and Validation

Knowledge capturing and reusing are major processes of knowledge management that deal with the elicitation of valuable knowledge via some techniques and methods for use in actual and further studies, projects, services, or products. The construction industry, as well, adopts and uses some of these concepts to improve various construction processes and stages. From pre-design to building delivery knowledge management principles and briefing frameworks have been implemented across project stakeholders: client, design teams, construction teams, consultants, and facility management teams. At pre-design and design stages, understanding the client’s needs and users’ knowledge are crucial for identifying and articulating the expected requirements and objectives. Due to underperforming results and missed goals and objectives, many projects finish with highly dissatisfied clients and loss of contracts for some organizations. Knowledge capturing has beneficial effects via its principles and methods on requirement elicitation and validation at the briefing stage between user, client and designer. This paper presents the importance and usage of knowledge capturing and reusing in briefing process at pre-design and design stages especially the involvement of client and user, and explores the techniques and technologies that are usable in briefing process for requirement elicitation

An Investigation on Benefit-Cost Analysis of Greenhouse Structures in Antalya

Significant population increase across the world, loss of cultivable land and increasing demand for food put pressure on agriculture. To meet the demand, greenhouses are built, which are, light structures with transparent cladding material in order to provide controlled microclimatic environment proper for plant production. Conceptually, greenhouses are similar with manufacturing buildings where a controlled environment for manufacturing and production have been provided and proper spaces for standardized production processes have been enabled. Parallel with the trends in the world, particularly in southern regions, greenhouse structures have been increasingly constructed and operated in Turkey. A significant number of greenhouses are located at Antalya. The satellite images demonstrated that for over last three decades, there has been a continuous invasion of greenhouses on all cultivable land. There are various researches and attempts for the improvement of greenhouse design and for increasing food production by decreasing required energy consumption. However, the majority of greenhouses in Turkey are very rudimentary structures where capital required for investment is low, but maintenance requirements are high when compared with new generation greenhouse structures. In this research paper, life-long capital requirements for construction and operation of greenhouse buildings in Antalya has been investigated by using benefit-cost analysis study

A Holmes and Doyle Bibliography, Volume 5: Periodical Articles--Secondary References, Alphabetical Listing

This bibliography is a work in progress. It attempts to update Ronald B. De Waal’s comprehensive bibliography, The Universal Sherlock Holmes, but does not claim to be exhaustive in content. New works are continually discovered and added to this bibliography. Readers and researchers are invited to suggest additional content. Volume 5 includes "passing" or "secondary" references, i.e. those entries that are passing in nature or contain very brief information or content

A Holmes and Doyle Bibliography, Volume 9: All Formats—Combined Alphabetical Listing

This bibliography is a work in progress. It attempts to update Ronald B. De Waal’s comprehensive bibliography, The Universal Sherlock Holmes, but does not claim to be exhaustive in content. New works are continually discovered and added to this bibliography. Readers and researchers are invited to suggest additional content. This volume contains all listings in all formats, arranged alphabetically by author or main entry. In other words, it combines the listings from Volume 1 (Monograph and Serial Titles), Volume 3 (Periodical Articles), and Volume 7 (Audio/Visual Materials) into a comprehensive bibliography. (There may be additional materials included in this list, e.g. duplicate items and items not yet fully edited.) As in the other volumes, coverage of this material begins around 1994, the final year covered by De Waal's bibliography, but may not yet be totally up-to-date (given the ongoing nature of this bibliography). It is hoped that other titles will be added at a later date. At present, this bibliography includes 12,594 items

2023, UMaine News Press Releases

This is a catalog of press releases put out by the University of Maine Division of Marketing and Communications between January 3, 2023 and November 1, 2023

A Holmes and Doyle Bibliography, Volume 6: Periodical Articles, Subject Listing, By De Waal Category

This bibliography is a work in progress. It attempts to update Ronald B. De Waal’s comprehensive bibliography, The Universal Sherlock Holmes, but does not claim to be exhaustive in content. New works are continually discovered and added to this bibliography. Readers and researchers are invited to suggest additional content. Volume 6 presents the periodical literature arranged by subject categories (as originally devised for the De Waal bibliography and slightly modified here)

The HBP1 tumor suppressor is a negative epigenetic regulator of MYCN driven neuroblastoma through interaction with the PRC2 complex

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