1,625 research outputs found
Regulation of Op18 during Spindle Assembly in Xenopus Egg Extracts
Oncoprotein 18 (Op18) is a microtubule-destabilizing protein that is negatively regulated by phosphorylation. To evaluate the role of the three Op18 phosphorylation sites in Xenopus (Ser 16, 25, and 39), we added wild-type Op18, a nonphosphorylatable triple Ser to Ala mutant (Op18-AAA), and to mimic phosphorylation, a triple Ser to Glu mutant (Op18-EEE) to egg extracts and monitored spindle assembly. Op18-AAA dramatically decreased microtubule length and density, while Op18-EEE did not significantly affect spindle microtubules. Affinity chromatography with these proteins revealed that the microtubule-destabilizing activity correlated with the ability of Op18 to bind tubulin. Since hyperphosphorylation of Op18 is observed upon addition of mitotic chromatin to extracts, we reasoned that chromatin-associated proteins might play a role in Op18 regulation. We have performed a preliminary characterization of the chromatin proteins recruited to DNA beads, and identified the Xenopus polo-like kinase Plx1 as a chromatin-associated kinase that regulates Op18 phosphorylation. Depletion of Plx1 inhibits chromatin-induced Op18 hyperphosphorylation and spindle assembly in extracts. Therefore, Plx1 may promote microtubule stabilization and spindle assembly by inhibiting Op18
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Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration.
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are associated with loss of nuclear transactive response DNA-binding protein 43 (TDP-43). Here we identify that TDP-43 regulates expression of the neuronal growth-associated factor stathmin-2. Lowered TDP-43 levels, which reduce its binding to sites within the first intron of stathmin-2 pre-messenger RNA, uncover a cryptic polyadenylation site whose utilization produces a truncated, non-functional mRNA. Reduced stathmin-2 expression is found in neurons trans-differentiated from patient fibroblasts expressing an ALS-causing TDP-43 mutation, in motor cortex and spinal motor neurons from patients with sporadic ALS and familial ALS with GGGGCC repeat expansion in the C9orf72 gene, and in induced pluripotent stem cell (iPSC)-derived motor neurons depleted of TDP-43. Remarkably, while reduction in TDP-43 is shown to inhibit axonal regeneration of iPSC-derived motor neurons, rescue of stathmin-2 expression restores axonal regenerative capacity. Thus, premature polyadenylation-mediated reduction in stathmin-2 is a hallmark of ALS-FTD that functionally links reduced nuclear TDP-43 function to enhanced neuronal vulnerability
Spatial and Temporal Sensing Limits of Microtubule Polarization in Neuronal Growth Cones by Intracellular Gradients and Forces
Neuronal growth cones are the most sensitive amongst eukaryotic cells in
responding to directional chemical cues. Although a dynamic microtubule
cytoskeleton has been shown to be essential for growth cone turning, the
precise nature of coupling of the spatial cue with microtubule polarization is
less understood. Here we present a computational model of microtubule
polarization in a turning neuronal growth cone (GC). We explore the limits of
directional cues in modifying the spatial polarization of microtubules by
testing the role of microtubule dynamics, gradients of regulators and
retrograde forces along filopodia. We analyze the steady state and transition
behavior of microtubules on being presented with a directional stimulus. The
model makes novel predictions about the minimal angular spread of the chemical
signal at the growth cone and the fastest polarization times. A regulatory
reaction-diffusion network based on the cyclic
phosphorylation-dephosphorylation of a regulator predicts that the receptor
signal magnitude can generate the maximal polarization of microtubules and not
feedback loops or amplifications in the network. Using both the
phenomenological and network models we have demonstrated some of the physical
limits within which the MT polarization system works in turning neuron.Comment: 7 figures and supplementary materia
Intrinsic structural disorder in cytoskeletal proteins.
Cytoskeleton, the internal scaffold of the cell, displays an exceptional combination of stability and dynamics. It is composed of three major filamentous networks, microfilaments (actin filaments), intermediate filaments (neurofilaments), and microtubules. Together, they ensure the physical and structural stability of the cell, whereby also mediating its large-scale structural rearrangements, motility, stress response, division, and internal transport. All three cytoskeletal systems are built upon the same basic design: they have a central repetitive scaffold assembled from folded building elements, surrounded and regulated by accessory regions/proteins that regulate its formation and mediate its countless interactions with its environment, serving to send regulatory signals to and from the cytoskeleton. Here, we elaborate on the idea that the opposing features of stability and dynamics are also manifest in the dichotomy of the structural status of its components, the core being highly structured and the accessory proteins/regions being highly disordered, and are responsible for most of the regulatory (post-translational) input promoting adaptive responses and providing dynamics necessary for each of the cytoskeletal systems. This pattern entails special consequences, in which the manifold functional advantages of structural disorder, most pronounced in regulatory and signaling functions, are all exploited by nature. (c) 2013 Wiley Periodicals, Inc
WAVE2 Protein Complex Coupled to Membrane and Microtubules
E-cadherin is one of the key molecules in the formation of cell-cell adhesion and interacts intracellularly with a group of proteins collectively named catenins, through which the E-cadherin-catenin complex is anchored to actin-based cytoskeletal components. Although cell-cell adhesion is often disrupted in cancer cells by either genetic or epigenetic alterations in cell adhesion molecules, disruption of cell-cell adhesion alone seems to be insufficient for the induction of cancer cell migration and invasion. A small GTP-binding protein, Rac1, induces the specific cellular protrusions lamellipodia via WAVE2, a member of WASP/WAVE family of the actin cytoskeletal regulatory proteins. Biochemical and pharmacological investigations have revealed that WAVE2 interacts with many proteins that regulate microtubule growth, actin assembly, and membrane targeting of proteins, all of which are necessary for directional cell migration through lamellipodia formation. These findings might have important implications for the development of effective therapeutic agents against cancer cell migration and invasion
Feedback Mechanism for Microtubule Length Regulation by Stathmin Gradients
We formulate and analyze a theoretical model for the regulation of
microtubule (MT) polymerization dynamics by the signaling proteins Rac1 and
stathmin. In cells, the MT growth rate is inhibited by cytosolic stathmin,
which, in turn, is inactivated by Rac1. Growing MTs activate Rac1 at the cell
edge, which closes a positive feedback loop. We investigate both tubulin
sequestering and catastrophe promotion as mechanisms for MT growth inhibition
by stathmin. For a homogeneous stathmin concentration in the absence of Rac1,
we find a switch-like regulation of the MT mean length by stathmin. For
constitutively active Rac1 at the cell edge, stathmin is deactivated locally,
which establishes a spatial gradient of active stathmin. In this gradient, we
find a stationary bimodal MT length distributions for both mechanisms of MT
growth inhibition by stathmin. One subpopulation of the bimodal length
distribution can be identified with fast growing and long pioneering MTs in the
region near the cell edge, which have been observed experimentally. The
feedback loop is closed through Rac1 activation by MTs. For tubulin
sequestering by stathmin, this establishes a bistable switch with two stable
states: one stable state corresponds to upregulated MT mean length and bimodal
MT length distributions, i.e., pioneering MTs; the other stable state
corresponds to an interrupted feedback with short MTs. Stochastic effects as
well as external perturbations can trigger switching events. For catastrophe
promoting stathmin we do not find bistability
Understanding the role of Merkel cell polyomavirus oncoproteins in the cellular transformation of mammalian Merkel cells
Merkel cell carcinoma (MCC) is a highly aggressive skin cancer of neuroendocrine origin with a high propensity for metastasis through the dermal lymphatic system. In 2008, Merkel cell polyomavirus (MCPyV) was discovered monoclonally integrated within the host genome of more than 80% of MCC tumours. MCPyV is known to contribute to the transformation and maintenance of MCC tumour cells through the expression of the Large and Small Tumour (LT and ST) antigens. To date, a number of mechanisms by which MCPyV T antigen expression promotes cell transformation and viral replication have been elucidated. Although, no studies have addressed the molecular mechanisms associated with MCPyV T antigen expression and the highly aggressive, metastatic nature of MCC.
Herein, a quantitative proteomic approach has been used to identify cellular proteins and gene ontology pathways that are differentially expressed upon MCPyV ST expression. This highlighted that MCPyV ST expression promotes the upregulation of cellular proteins involved in microtubule-associated cytoskeletal organisation and dynamics.
Further analysis, has demonstrated that MCPyV ST promotes cell motility, migration and invasion and that MCPyV ST-induced microtubule destabilisation is fundamental for this phenotype. Bioinformatical analysis highlighted the upregulation of stathmin, a microtubule destabilising protein, which is required for MCPyV ST-induced cell motility. Furthermore, through protein interaction studies and cell motility assays, the cellular phosphatase catalytic subunit PP4C has been implicated in the regulation of this process.
Herein, these findings present the first study into the metastatic nature of MCC and the underlying mechanisms by which MCPyV T antigen expression can promote cell migration. This in turn should allow for enhanced therapeutic studies and chemotherapy regimes, in order to improve current treatments of MCC
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