Detection of HPV in cancerous and non-cancerous esophageal tissues from Turkmen-Sahra, Iran

The aim of this study were to assess the presence of HPV esophageal infection among Iranian Turkmen who live in an area located in the cancer belt in Asia. The specimens derived from 120 patients previously diagnosed for Squamous Cell Carcinoma (SCC) non-cancerous tissue derived from esophagus. All specimens were examined for the presence of HPV DNA PCR was utilized to amplify a 150 bp segment of HPV L1 gene using the consensus primers. The amplified region was subsequently sequenced to identify the HPV genotypes. The HPV DNA was detected in 49.4% of patients with SCC, and 58% of non-cancerous tissue of esophagus. The positive samples included HPV-16 (46.6%), HPV-6 (24.6%), HPV-66 (8.2%), HPV-52 (4.1%), HPV-18 (2.7%); 14% of cases were positive for more than one type of HPV. The results confirm the presence of HPV in both esophageal cancerous and non-cancerous tissues. These results imply two different interpretations: 1) Due to non-significant difference between the rate of HPV positive in cancerous and non-cancerous tissues, HPV has no important role in esophageal cancers, which is less probable. 2). Because of a highly incidence of esophageal cancer in Turkmen-Sahra region, the HPV is a possible etiologic agent in esophageal carcinogenesis, most probably acting synergistically with physical, chemical, and/or nutritional factors that have previously been found to be related to this malignancy in Turkmen-Sahra. © 2006 Academic Journals Inc., USA

Instructions for laboratory training in Special Microbiology and Virology for students of Faculty of Dentistry

МИКРОБИОЛОГИЯМЕТОДИЧЕСКИЕ УКАЗАНИЯЛАБОРАТОРНЫЕ РАБОТЫВИРУСОЛОГИЯИНОСТРАННЫЕ СТУДЕНТЫПРАКТИЧЕСКИЕ НАВЫКИСодержатся план, программа практических занятий и основные практические навыки по общей микробиологии и вирусологии

Medical Microbiology, Virology & Immunology. Pt. 1. General Microbiology & Medical Immunology

КУРСЫ ЛЕКЦИЙМИКРОБИОЛОГИЯВИРУСОЛОГИЯИММУНОЛОГИЯАЛЛЕРГОЛОГИЯ И ИММУНОЛОГИЯВ первую часть курса лекций включены разделы по общей микробиологии и медицинской иммунологии

Medical Microbiology, Virology & Immunology. Pt. 1. General Microbiology & Medical Immunology

КУРСЫ ЛЕКЦИЙМИКРОБИОЛОГИЯВИРУСОЛОГИЯИММУНОЛОГИЯАЛЛЕРГОЛОГИЯ И ИММУНОЛОГИЯВ первую часть курса лекций включены разделы по общей микробиологии и медицинской иммунологии

Selective inhibition of T suppressor-cell function by a monosaccharide

Interactions between regulatory T lymphocytes and other cells are assumed to occur at the level of the cell surface. T cells which suppress the generation of specifically effector cells have been described as having antigenic, idiotypic, allotypic and I-region specificity1−4. Other T suppressor cells generated by in vitro cultivation with or without mitogenic stimulation5,6 have suppressive activity for T and B cells but no specificity can be assigned to them. These T suppressor cells (Ts) inhibit various lymphoid functions—this either reflects their polyclonal origin or indicates that the structures recognized by the Ts receptors must be common for many cell types. Carbohydrates on cell membrane-inserted glycoproteins or glycolipids might function as specific ligands for recognition by cellular receptors or soluble factors. Almost all cell-surface proteins of mammalian cells are glycosylated. There is evidence for lectin-like carbohydrate binding proteins not only in plants7 but also in toxins8, viruses9, prokaryotic cells10 and even mammalian cells, including T cells11. A functional role for these lectin-like proteins has been described for slime moulds and suggested for the selective association of embryonic cells12,13. We report here that addition of a monosaccharide can counteract the effect of T suppressor cells during the generation of alloreactive cytotoxic T cells (CTLs) in vitro

Pengaruh Pemberian Ekstrak Etanol Daun Mirabilis jalapa terhadap Pertumbuhan Vibrio cholerae (The Effect of Mirabilis jalapa's Leaves Ethanolic Extract against the Growth of Vibrio cholerae)

Vibrio cholerae is one of the most common bacteria causing acute diarrhea. First-line treatment of diarrhea is tetracycline. However, the drug has some side effects. Therefore it is necessary to have an alternative therapy for V. cholerae especially from herbs. The purpose of this study was to determine the ability and the minimal inhibitory concentration (MIC) of Mirabilis jalapa leaves extract in inhibiting the growth of V. cholerae in in vitro method. This research used a Quasi Experimental Design with Post Test Only Control Group Design. The method of this study used diffusion method on Mueller Hinton Agar. The sample used was V. cholerae culture from the Laboratory of Microbiology Medical Faculty Brawijaya University. The inhibition growth of V. cholerae was observed start at a concentration of 1 mg/ml and increased by increasing concentrations of the extract. Spearman correlation test showed value p=0.000 and r=0.993, indicating significant correlation between the leaves extract of M. jalapa and V. cholerae diameter inhibition zone. These results indicated that M. jalapa leaves extract could inhibit the growth of V. cholerae and had qualitatively MIC 1 mg/ml, while quantitatively 0.2 mg/ml.   Keywords: microbiology, Mirabilis jalapa, Vibrio cholerae, in vitro &nbsp