in situ experiment

The increasing amount of plastic littered into the sea may provide a new substratum for benthic organisms. These marine fouling communities on plastic have not received much scientific attention. We present, to our knowledge, the first comprehensive analysis of their macroscopic community composition, their primary production and the polymer degradation comparing conventional polyethylene (PE) and a biodegradable starch-based plastic blend in coastal benthic and pelagic habitats in the Mediterranean Sea. The biomass of the fouling layer increased significantly over time and all samples became heavy enough to sink to the seafloor. The fouling communities, consisting of 21 families, were distinct between habitats, but not between polymer types. Positive primary production was measured in the pelagic, but not in the benthic habitat, suggesting that large accumulations of floating plastic could pose a source of oxygen for local ecosystems, as well as a carbon sink. Contrary to PE, the biodegradable plastic showed a significant loss of tensile strength and disintegrated over time in both habitats. These results indicate that in the marine environment, biodegradable polymers may disintegrate at higher rates than conventional polymers. This should be considered for the development of new materials, environmental risk assessment and waste management strategies

Influence of bioturbation on denitrification activity in Mediterranean coastal sediments:an in situ experimental approach

An in situ experiment was conducted in the French Mediterranean littoral (Gulf of Fos) from July 1993 to January 1994 using controls without macrofauna or natural sediments. After 1, 4 and 6 mo, sediment reworking and denitrification activities (natural and potential rates) were studied. The bacterial processes were stimulated by the bioturbating activity of the autochthonous infauna. The natural and potential denitrification rates were 160 and 280% higher, respectively, than in the controls. The increase of denitrification, occurring at different depths in the sediment with respect to time, was directly dependent on the macrofaunal activity

The Laboratory of Intervention in Architecture in situ / experiment

UIDB/04647/2020 UIDP/04647/2020This chapter discusses the role of collaborative practices for the production of public spaces. The growing complexity of urban contexts, due to changes in the production model and in urban activities is a challenge for scholars, technicians and residents. In this context collaborative practices have been a common approach in urban interventions in the last decades. The text presents eight editions of the project Laboratory of Intervention in Architecture in situ/, from 2012 to 2018. It is a project of intervention in local public spaces with the partnerships of the Municipality of Almada and numerous local institutions, using collaborative practices as methodological approach. In situ/ is also a learning process, as it is coordinated by a research centre, and has a strong concern with the educational aspects of these living labs and with urban research. Different research themes have been addressed – such as informal neighbourhoods, old industrial areas or coastal environmental protection – but they all have a common ground: how can communities (be they academic, local or institutional) contribute to solve territorial problems in transitional urban contexts using public space as a mediation and negotiation tool? In situ/ projects aim to promote innovative teaching and learning experiences outside the academic context, in multidisciplinary and diverse social and cultural contexts, but also to investigate issues of the city in transition and intervene in real contexts of action, promoting diversified partnerships with local entities, designing and building solutions to the challenges of contemporary metropolitan contexts, thus contributing to improve the quality of life of the populations. Along the same lines, this chapter aims to present the laboratories’ methodological approach, focusing on the organizational aspects, the importance of all the actors involved and of the learning experience.publishersversionpublishe

Can We Model Driver Perceptions? An In-Situ Experiment in Real-World Conditions

ABSTRACTIt is clear that perceptions play a significant role in traveler decisions. Consequently, traveler perceptions are a corner stone in the feasibility of traveler information systems; for traveler information systems are only valuable if the drivers are incapable of accurately acquiring the provided information on their own, and if the provided information is relevant for the drivers' decision criteria. Accuracy of traveler perceptions has been repeatedly researched in public transportation, and has been found to vary according to different reasons. However, in spite of the clear significance of traveler perceptions, minimal effort has been put into modeling it. Almost all travel behavior models are based on traveler experiences, which are assumed to reflect traveler perceptions via the addition of some random error component. This works introduces an alternative approach: instead of adding an error component to represent driver perceptions, it proposes to model driver perceptions. This work is based on a real-world route choice experiment of a sample of 20 drivers who made more than 2,000 real-world route choices. Each of the drivers' experiences, perceptions, and choices were recorded, analyzed and cross examined. The paper demonstrates that: i) driver experiences are different from driver perceptions, ii) driver perceptions explain driver choices better than driver experiences, iii) it is possible to model and predict driver perceptions of travel distance, time and speed

Hydrocarbon influence on denitrification in bioturbated Mediterranean coastal sediments

An in situ experiment has been carried out inbioturbated Mediterranean coastal marine sediments (Gulfof Fos) in order to study the influence of hydrocarbons ondenitrification after 1, 4 and 6 months. In theabsence of hydrocarbons in the control sediments, the presenceof macrofauna stimulated denitrificationby 160%. This stimulation is induced by sediment reworkingthat favours both direct NO-3 supply fromthe water column and the penetration of O{2}, which in turnstimulated nitrification, the other source ofNO-3 in sediment. The presence of hydrocarbons in theexperimental sediments either stimulated orinhibited the denitrification. The denitrification response tothe presence of hydrocarbon is dependent onthe quantity of matter buried by the macrofauna activity. Insmall quantities, the organic matter relatedto hydrocarbons 120% enhanced the denitrification compared tothe controls. On the other hand, whenburied hydrocarbon concentrations were higher (>100 mgsaturated hydrocarbon fraction kg-1 drysediment), the denitrification was inhibited.On the basis of the results obtained, a descriptive model ofthe patterns of denitrification in relation to the presence ofmacrofauna and the distribution of hydrocarbons in sediments is proposed

In Vitro Versus in Situ Ruminal Biohydrogenation of Unsaturated Fatty Acids from a Raw or Extruded Mixture of Ground Canola Seed/Canola Meal

Raw or extruded blends of ground canola seeds and canola meal were used to compare in vitro and in situ lag times and rates of disappearance due to ruminal biohydrogenation of unsaturated fatty acids. The in situ study resulted in higher lag times for biohydrogenation for polyunsaturated fatty acids and lower rates of biohydrogenation of unsaturated fatty acids than the in vitro study, so the in situ biohydrogenation of polyunsaturated fatty acids was not complete at 24 h of incubation. With both methods, rates of biohydrogenation of polyunsaturated fatty acids were higher than for cis-9C18:1. Extrusion did not affect the rate of biohydrogenation of cis-9C18:1, but resulted in higher rates of biohydrogenation of polyunsaturated fatty acids with higher proportions of trans intermediates of biohydrogenation at 4 h of incubation in vitro and at 8 h of incubation in situ. These results suggest that extrusion affects the isomerization of polyunsaturated fatty acids, rather than the hydrogenation steps. In conclusion, in vitro and in situ methods can both show differences of ruminal metabolism of unsaturated fatty acids due to processing, but the methods provide very different estimates of the rates of disappearance due to biohydrogenation

In Situ Experiment and Modelling of RC-Structure using Ambient vibration and Timoshenko Beam

International audienceRecently, several experiments were reported using ambient vibration surveys in buildings to estimate the modal parameters of buildings. Their modal properties are full of relevant information concerning its dynamic behaviour in its elastic domain. The main scope of this paper is to determine relevant, though simple, beam modelling whose validity could be easily checked with experimental data. In this study, we recorded ambient vibrations in 3 buildings in Grenoble selected because of their vertical structural homogeneity. First, a set of recordings was done using a 18 channels digital acquisition system (CityShark) connected to six 3C Lennartz 5s sensors. We used the Frequency Domain Decomposition (FDD) technique to extract the modal parameters of these buildings. Second, it is shown in the following that the experimental quasi-elastic behaviour of such structure can be reduced to the behaviour of a vertical continuous Timoshenko beam. A parametric study of this beam shows that a bijective relation exists between the beam parameters and its eigenfrequencies distribution. Consequently, the Timoshenko beam parameters can be estimated from the experimental sequence of eigenfrequencies. Having the beam parameters calibrated by the in situ data, the reliability of the modelling is checked by complementary comparisons. For this purpose, the mode shapes and eigenfrequencies of higher modes are calculated and compared to the experimental data. A good agreement is also obtained. In addition, the beam model integrates in a very synthetic way the essential parameters of the dynamic behaviour

Influence of experimental conditions on sound pleasantness evaluations

ICA 2016, 22nd International Congress on Acoustics, BUENOS AIRES, ARGENTINE, 05-/09/2016 - 09/09/2016Being able to characterize and estimate the urban sound perception is a key point to improve the city dwellers environmental quality. In the past decade, various studies have focused on collecting perceived global sound pleasantness at specific locations. Some of them were carried out on field in order to evaluate the soundscape perception of the participants directly in their context. Other studies were realized in laboratory to better control the stimuli and to increase the number of participants who were subjected to the same sound environment. Most of the laboratory experiments are done in large or semi-anechoic chamber with calibrated and highly realistic audio reproduction in order to respect the ecological validity of the experiment. On one hand, even with a high immersive level, the laboratory context is not as rich as the field context and the two types of experiment could lead to different results. On the other hand, few studies exist showing the influence of decreasing ecological validity for the same experience. This work presents a short statistical analysis of perceptive evaluations of ten urban locations under 4 different test conditions. First, evaluations are carried out directly in-situ in the city of Paris. Then audio-visual recordings of these locations are evaluated in three different experimental conditions: (i) in a well-controlled acoustic laboratory in Paris region with French people, (ii) in an acoustic laboratory in Buenos Aires with Argentinean participants and lowest immersive conditions, (iii) in a habitational room with Argentinean participants and subjective calibration. The study reveals that both the 'country' factor and the experimental conditions in laboratory do not show any significant impact on the perceived sound pleasantness and perceived loudness assessments