Automatic Detection of Calibration Grids in Time-of-Flight Images

It is convenient to calibrate time-of-flight cameras by established methods, using images of a chequerboard pattern. The low resolution of the amplitude image, however, makes it difficult to detect the board reliably. Heuristic detection methods, based on connected image-components, perform very poorly on this data. An alternative, geometrically-principled method is introduced here, based on the Hough transform. The projection of a chequerboard is represented by two pencils of lines, which are identified as oriented clusters in the gradient-data of the image. A projective Hough transform is applied to each of the two clusters, in axis-aligned coordinates. The range of each transform is properly bounded, because the corresponding gradient vectors are approximately parallel. Each of the two transforms contains a series of collinear peaks; one for every line in the given pencil. This pattern is easily detected, by sweeping a dual line through the transform. The proposed Hough-based method is compared to the standard OpenCV detection routine, by application to several hundred time-of-flight images. It is shown that the new method detects significantly more calibration boards, over a greater variety of poses, without any overall loss of accuracy. This conclusion is based on an analysis of both geometric and photometric error.Comment: 11 pages, 11 figures, 1 tabl

A Patch-Based Method for Repetitive and Transient Event Detection in Fluorescence Imaging

Automatic detection and characterization of molecular behavior in large data sets obtained by fast imaging in advanced light microscopy become key issues to decipher the dynamic architectures and their coordination in the living cell. Automatic quantification of the number of sudden and transient events observed in fluorescence microscopy is discussed in this paper. We propose a calibrated method based on the comparison of image patches expected to distinguish sudden appearing/vanishing fluorescent spots from other motion behaviors such as lateral movements. We analyze the performances of two statistical control procedures and compare the proposed approach to a frame difference approach using the same controls on a benchmark of synthetic image sequences. We have then selected a molecular model related to membrane trafficking and considered real image sequences obtained in cells stably expressing an endocytic-recycling trans-membrane protein, the Langerin-YFP, for validation. With this model, we targeted the efficient detection of fast and transient local fluorescence concentration arising in image sequences from a data base provided by two different microscopy modalities, wide field (WF) video microscopy using maximum intensity projection along the axial direction and total internal reflection fluorescence microscopy. Finally, the proposed detection method is briefly used to statistically explore the effect of several perturbations on the rate of transient events detected on the pilot biological model

Calibration and Sensitivity Analysis of a Stereo Vision-Based Driver Assistance System

Az http://intechweb.org/ alatti "Books" fül alatt kell rákeresni a "Stereo Vision" címre és az 1. fejezetre

Analysis of Signal Decomposition and Stain Separation methods for biomedical applications

Nowadays, the biomedical signal processing and classification and medical image interpretation play an essential role in the detection and diagnosis of several human diseases. The problem of high variability and heterogeneity of information, which is extracted from digital data, can be addressed with signal decomposition and stain separation techniques which can be useful approaches to highlight hidden patterns or rhythms in biological signals and specific cellular structures in histological color images, respectively. This thesis work can be divided into two macro-sections. In the first part (Part I), a novel cascaded RNN model based on long short-term memory (LSTM) blocks is presented with the aim to classify sleep stages automatically. A general workflow based on single-channel EEG signals is developed to enhance the low performance in staging N1 sleep without reducing the performances in the other sleep stages (i.e. Wake, N2, N3 and REM). In the same context, several signal decomposition techniques and time-frequency representations are deployed for the analysis of EEG signals. All extracted features are analyzed by using a novel correlation-based timestep feature selection and finally the selected features are fed to a bidirectional RNN model. In the second part (Part II), a fully automated method named SCAN (Stain Color Adaptive Normalization) is proposed for the separation and normalization of staining in digital pathology. This normalization system allows to standardize digitally, automatically and in a few seconds, the color intensity of a tissue slide with respect to that of a target image, in order to improve the pathologist’s diagnosis and increase the accuracy of computer-assisted diagnosis (CAD) systems. Multiscale evaluation and multi-tissue comparison are performed for assessing the robustness of the proposed method. In addition, a stain normalization based on a novel mathematical technique, named ICD (Inverse Color Deconvolution) is developed for immunohistochemical (IHC) staining in histopathological images. In conclusion, the proposed techniques achieve satisfactory results compared to state-of-the-art methods in the same research field. The workflow proposed in this thesis work and the developed algorithms can be employed for the analysis and interpretation of other biomedical signals and for digital medical image analysis

Optimal state estimation for cavity optomechanical systems

We demonstrate optimal state estimation for a cavity optomechanical system through Kalman filtering. By taking into account nontrivial experimental noise sources, such as colored laser noise and spurious mechanical modes, we implement a realistic state-space model. This allows us to obtain the conditional system state, i.e., conditioned on previous measurements, with minimal least-square estimation error. We apply this method for estimating the mechanical state, as well as optomechanical correlations both in the weak and strong coupling regime. The application of the Kalman filter is an important next step for achieving real-time optimal (classical and quantum) control of cavity optomechanical systems.Comment: replaced with published version, 5+12 page

Road environment modeling using robust perspective analysis and recursive Bayesian segmentation

Recently, vision-based advanced driver-assistance systems (ADAS) have received a new increased interest to enhance driving safety. In particular, due to its high performance–cost ratio, mono-camera systems are arising as the main focus of this field of work. In this paper we present a novel on-board road modeling and vehicle detection system, which is a part of the result of the European I-WAY project. The system relies on a robust estimation of the perspective of the scene, which adapts to the dynamics of the vehicle and generates a stabilized rectified image of the road plane. This rectified plane is used by a recursive Bayesian classi- fier, which classifies pixels as belonging to different classes corresponding to the elements of interest of the scenario. This stage works as an intermediate layer that isolates subsequent modules since it absorbs the inherent variability of the scene. The system has been tested on-road, in different scenarios, including varied illumination and adverse weather conditions, and the results have been proved to be remarkable even for such complex scenarios