ASTErIsM - Application of topometric clustering algorithms in automatic galaxy detection and classification

We present a study on galaxy detection and shape classification using topometric clustering algorithms. We first use the DBSCAN algorithm to extract, from CCD frames, groups of adjacent pixels with significant fluxes and we then apply the DENCLUE algorithm to separate the contributions of overlapping sources. The DENCLUE separation is based on the localization of pattern of local maxima, through an iterative algorithm which associates each pixel to the closest local maximum. Our main classification goal is to take apart elliptical from spiral galaxies. We introduce new sets of features derived from the computation of geometrical invariant moments of the pixel group shape and from the statistics of the spatial distribution of the DENCLUE local maxima patterns. Ellipticals are characterized by a single group of local maxima, related to the galaxy core, while spiral galaxies have additional ones related to segments of spiral arms. We use two different supervised ensemble classification algorithms, Random Forest, and Gradient Boosting. Using a sample of ~ 24000 galaxies taken from the Galaxy Zoo 2 main sample with spectroscopic redshifts, and we test our classification against the Galaxy Zoo 2 catalog. We find that features extracted from our pipeline give on average an accuracy of ~ 93%, when testing on a test set with a size of 20% of our full data set, with features deriving from the angular distribution of density attractor ranking at the top of the discrimination power.Comment: 20 pages, 13 Figures, 8 Tables, Accepted for publication in the Monthly Notices of the Royal Astronomical Societ

Transcription Factor-DNA Binding Via Machine Learning Ensembles

We present ensemble methods in a machine learning (ML) framework combining predictions from five known motif/binding site exploration algorithms. For a given TF the ensemble starts with position weight matrices (PWM's) for the motif, collected from the component algorithms. Using dimension reduction, we identify significant PWM-based subspaces for analysis. Within each subspace a machine classifier is built for identifying the TF's gene (promoter) targets (Problem 1). These PWM-based subspaces form an ML-based sequence analysis tool. Problem 2 (finding binding motifs) is solved by agglomerating k-mer (string) feature PWM-based subspaces that stand out in identifying gene targets. We approach Problem 3 (binding sites) with a novel machine learning approach that uses promoter string features and ML importance scores in a classification algorithm locating binding sites across the genome. For target gene identification this method improves performance (measured by the F1 score) by about 10 percentage points over the (a) motif scanning method and (b) the coexpression-based association method. Top motif outperformed 5 component algorithms as well as two other common algorithms (BEST and DEME). For identifying individual binding sites on a benchmark cross species database (Tompa et al., 2005) we match the best performer without much human intervention. It also improved the performance on mammalian TFs. The ensemble can integrate orthogonal information from different weak learners (potentially using entirely different types of features) into a machine learner that can perform consistently better for more TFs. The TF gene target identification component (problem 1 above) is useful in constructing a transcriptional regulatory network from known TF-target associations. The ensemble is easily extendable to include more tools as well as future PWM-based information.Comment: 33 page

Phenotypic landscape inference reveals multiple evolutionary paths to C4_4 photosynthesis

C$_4$ photosynthesis has independently evolved from the ancestral C$_3$ pathway in at least 60 plant lineages, but, as with other complex traits, how it evolved is unclear. Here we show that the polyphyletic appearance of C$_4$ photosynthesis is associated with diverse and flexible evolutionary paths that group into four major trajectories. We conducted a meta-analysis of 18 lineages containing species that use C$_3$, C$_4$, or intermediate C$_3$-C$_4$ forms of photosynthesis to parameterise a 16-dimensional phenotypic landscape. We then developed and experimentally verified a novel Bayesian approach based on a hidden Markov model that predicts how the C$_4$ phenotype evolved. The alternative evolutionary histories underlying the appearance of C$_4$ photosynthesis were determined by ancestral lineage and initial phenotypic alterations unrelated to photosynthesis. We conclude that the order of C$_4$ trait acquisition is flexible and driven by non-photosynthetic drivers. This flexibility will have facilitated the convergent evolution of this complex trait