Capacitance immunosensors for the early detection of circulating cancer biomarkers

I have been successful in improving and developing a homemade device based on a three-electrodes electrochemical redout setup thanks to it, we are able to perform measurements of DNA-hybridization, in real-time, from probe ssDNA-SAMs coupled to gold coated sensor surfaces. The measurements were carried out, in pure saline buffer solution, on a large range of concentrations of complementary-DNA strands (from 1 pM to 100 nM), monitoring the differential capacitance at the Working Electrode versus the incubation time. The studies on kinetics, modeled using the Langmuir adsorption model, not only give us important information on the kinetics itself but they allow us to detect eventual mismatches along the DNA-sequence target proving to be sensitive to the position of the mismatch with respect to the surface of the device or to define, in human extract and plasma, the unknown concentration of a specific miRNA-target connected to the heart failure taking into account the hindrances carried by the Argonaute proteins in which the miRNA are inglobed. This goal was achieved by performing a calibration curve on experiments of DNA/DNA hybridization performed in a simple saline buffer. The results were then confirmed using a real time qPCR by our partners in MD D. Cesselli's and MD A.P. Beltrami's group at University of Udine. Another strand of my PhD project concerns the detection of more complex components such as proteins or single-domain antibodies (e.g. VHH fragments)\u2013DNA conjugates, with the final purpose of the detection of circulating tumor cells (CTCs) not only in pure saline buffer but also in human serum. In particular, we have focused on the detection of the protein HER2 whose overexpression is connected to certain aggressive types of breast cancer. In addition, the systematic characterization of the device caught our attention, and it was developed by performing measurements of Self Assembled Monolayer (SAM) detection, carried out in different physiological buffers (KCl, NaCl, MgCl2, PBS, etc.), in different probe-density conditions and applying different potential in order to have a more comprehensive understanding of the phenomena occurring at the electrode/electrolyte interface. Studies that have led to the implementation of a theoretical model, able to provide an acceptable physical explanation of the biorecognition events of interest

Constraint analysis for DSP code generation

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Visualization of dynamic multidimensional and hierarchical datasets

When it comes to tools and techniques designed to help understanding complex abstract data, visualization methods play a prominent role. They enable human operators to lever age their pattern finding, outlier detection, and questioning abilities to visually reason about a given dataset. Many methods exist that create suitable and useful visual represen tations of static abstract, non-spatial, data. However, for temporal abstract, non-spatial, datasets, in which the data changes and evolves through time, far fewer visualization tech niques exist. This thesis focuses on the particular cases of temporal hierarchical data representation via dynamic treemaps, and temporal high-dimensional data visualization via dynamic projec tions. We tackle the joint question of how to extend projections and treemaps to stably, accurately, and scalably handle temporal multivariate and hierarchical data. The literature for static visualization techniques is rich and the state-of-the-art methods have proven to be valuable tools in data analysis. Their temporal/dynamic counterparts, however, are not as well studied, and, until recently, there were few hierarchical and high-dimensional methods that explicitly took into consideration the temporal aspect of the data. In addi tion, there are few or no metrics to assess the quality of these temporal mappings, and even fewer comprehensive benchmarks to compare these methods. This thesis addresses the abovementioned shortcomings. For both dynamic treemaps and dynamic projections, we propose ways to accurately measure temporal stability; we eval uate existing methods considering the tradeoff between stability and visual quality; and we propose new methods that strike a better balance between stability and visual quality than existing state-of-the-art techniques. We demonstrate our methods with a wide range of real-world data, including an application of our new dynamic projection methods to support the analysis and classification of hyperkinetic movement disorder data.Quando se trata de ferramentas e técnicas projetadas para ajudar na compreensão dados abstratos complexos, métodos de visualização desempenham um papel proeminente. Eles permitem que os operadores humanos alavanquem suas habilidades de descoberta de padrões, detecção de valores discrepantes, e questionamento visual para a raciocinar sobre um determinado conjunto de dados. Existem muitos métodos que criam representações visuais adequadas e úteis de para dados estáticos, abstratos, e não-espaciais. No entanto, para dados temporais, abstratos, e não-espaciais, isto é, dados que mudam e evoluem no tempo, existem poucas técnicas apropriadas. Esta tese concentra-se nos casos específicos de representação temporal de dados hierárquicos por meio de treemaps dinâmicos, e visualização temporal de dados de alta dimen sionalidade via projeções dinâmicas. Nós abordar a questão conjunta de como estender projeções e treemaps de forma estável, precisa e escalável para lidar com conjuntos de dados hierárquico-temporais e multivariado-temporais. Em ambos os casos, a literatura para técnicas estáticas é rica e os métodos estado da arte provam ser ferramentas valiosas em análise de dados. Suas contrapartes temporais/dinâmicas, no entanto, não são tão bem estudadas e, até recentemente, existiam poucos métodos hierárquicos e de alta dimensão que explicitamente levavam em consideração o aspecto temporal dos dados. Além disso, existiam poucas métricas para avaliar a qualidade desses mapeamentos visuais temporais, e ainda menos benchmarks abrangentes para comparação esses métodos. Esta tese aborda as deficiências acima mencionadas para treemaps dinâmicos e projeções dinâmicas. Propomos maneiras de medir com precisão a estabilidade temporal; avalia mos os métodos existentes, considerando o compromisso entre estabilidade e qualidade visual; e propomos novos métodos que atingem um melhor equilíbrio entre estabilidade e a qualidade visual do que as técnicas estado da arte atuais. Demonstramos nossos mé todos com uma ampla gama de dados do mundo real, incluindo uma aplicação de nossos novos métodos de projeção dinâmica para apoiar a análise e classificação dos dados de transtorno de movimentos

The Second Conference on Lunar Bases and Space Activities of the 21st Century, volume 1

These papers comprise a peer-review selection of presentations by authors from NASA, LPI industry, and academia at the Second Conference (April 1988) on Lunar Bases and Space Activities of the 21st Century, sponsored by the NASA Office of Exploration and the Lunar Planetary Institute. These papers go into more technical depth than did those published from the first NASA-sponsored symposium on the topic, held in 1984. Session topics covered by this volume include (1) design and operation of transportation systems to, in orbit around, and on the Moon, (2) lunar base site selection, (3) design, architecture, construction, and operation of lunar bases and human habitats, and (4) lunar-based scientific research and experimentation in astronomy, exobiology, and lunar geology

The Second Conference on Lunar Bases and Space Activities of the 21st Century, volume 2

These 92 papers comprise a peer-reviewed selection of presentations by authors from NASA, the Lunar and Planetary Institute (LPI), industry, and academia at the Second Conference on Lunar Bases and Space Activities of the 21st Century. These papers go into more technical depth than did those published from the first NASA-sponsored symposium on the topic, held in 1984. Session topics included the following: (1) design and operation of transportation systems to, in orbit around, and on the Moon; (2) lunar base site selection; (3) design, architecture, construction, and operation of lunar bases and human habitats; (4) lunar-based scientific research and experimentation in astronomy, exobiology, and lunar geology; (5) recovery and use of lunar resources; (6) environmental and human factors of and life support technology for human presence on the Moon; and (7) program management of human exploration of the Moon and space

Canada's Residential Schools: The History, Part 2 1939 to 2000

Summary of the final report of the Truth and Reconciliation of Canada