A century of enzyme kinetics. Should we believe in the Km and vmax estimates?

The application of the quasi-steady-state approximation (QSSA) in biochemical kinetics allows the reduction of a complex biochemical system with an initial fast transient into a simpler system. The simplified system yields insights into the behavior of the biochemical reaction, and analytical approximations can be obtained to determine its kinetic parameters. However, this process can lead to inaccuracies due to the inappropriate application of the QSSA. Here we present a number of approximate solutions and determine in which regions of the initial enzyme and substrate concentration parameter space they are valid. In particular, this illustrates that experimentalists must be careful to use the correct approximation appropriate to the initial conditions within the parameter space

Analog, hybrid, and digital simulation

Analog, hybrid, and digital computerized simulation technique

Reliability in Constrained Gauss-Markov Models: An Analytical and Differential Approach with Applications in Photogrammetry

This report was prepared by Jackson Cothren, a graduate research associate in the Department of Civil and Environmental Engineering and Geodetic Science at the Ohio State University, under the supervision of Professor Burkhard Schaffrin.This report was also submitted to the Graduate School of the Ohio State University as a dissertation in partial fulfillment of the requirements for the Ph.D. degree.Reliability analysis explains the contribution of each observation in an estimation model to the overall redundancy of the model, taking into account the geometry of the network as well as the precision of the observations themselves. It is principally used to design networks resistant to outliers in the observations by making the outliers more detectible using standard statistical tests.It has been studied extensively, and principally, in Gauss- Markov models. We show how the same analysis may be extended to various constrained Gauss-Markov models and present preliminary work for its use in unconstrained Gauss-Helmert models. In particular, we analyze the prominent reliability matrix of the constrained model to separate the contribution of the constraints to the redundancy of the observations from the observations themselves. In addition, we make extensive use of matrix differential calculus to find the Jacobian of the reliability matrix with respect to the parameters that define the network through both the original design and constraint matrices. The resulting Jacobian matrix reveals the sensitivity of reliability matrix elements highlighting weak areas in the network where changes in observations may result in unreliable observations. We apply the analytical framework to photogrammetric networks in which exterior orientation parameters are directly observed by GPS/INS systems. Tie-point observations provide some redundancy and even a few collinear tie-point and tie-point distance constraints improve the reliability of these direct observations by as much as 33%. Using the same theory we compare networks in which tie-points are observed on multiple images (n-fold points) and tie-points are observed in photo pairs only (two-fold points). Apparently, the use of two-fold tiepoints does not significantly degrade the reliability of the direct exterior observation observations. Coplanarity constraints added to the common two-fold points do not add significantly to the reliability of the direct exterior orientation observations. The differential calculus results may also be used to provide a new measure of redundancy number stability in networks. We show that a typical photogrammetric network with n-fold tie-points was less stable with respect to at least some tie-point movement than an equivalent network with n-fold tie-points decomposed into many two-fold tie-points

Algorithms and Data Structures for Multi-Adaptive Time-Stepping

Multi-adaptive Galerkin methods are extensions of the standard continuous and discontinuous Galerkin methods for the numerical solution of initial value problems for ordinary or partial differential equations. In particular, the multi-adaptive methods allow individual and adaptive time steps to be used for different components or in different regions of space. We present algorithms for efficient multi-adaptive time-stepping, including the recursive construction of time slabs and adaptive time step selection. We also present data structures for efficient storage and interpolation of the multi-adaptive solution. The efficiency of the proposed algorithms and data structures is demonstrated for a series of benchmark problems.Comment: ACM Transactions on Mathematical Software 35(3), 24 pages (2008

Discrete spherical means of directional derivatives and Veronese maps

We describe and study geometric properties of discrete circular and spherical means of directional derivatives of functions, as well as discrete approximations of higher order differential operators. For an arbitrary dimension we present a general construction for obtaining discrete spherical means of directional derivatives. The construction is based on using the Minkowski's existence theorem and Veronese maps. Approximating the directional derivatives by appropriate finite differences allows one to obtain finite difference operators with good rotation invariance properties. In particular, we use discrete circular and spherical means to derive discrete approximations of various linear and nonlinear first- and second-order differential operators, including discrete Laplacians. A practical potential of our approach is demonstrated by considering applications to nonlinear filtering of digital images and surface curvature estimation

On the Szeg\"o-Asymptotics for Doubly-Dispersive Gaussian Channels

We consider the time-continuous doubly-dispersive channel with additive Gaussian noise and establish a capacity formula for the case where the channel correlation operator is represented by a symbol which is periodic in time and fulfills some further integrability and smoothness conditions. The key to this result is a new Szeg\"o formula for certain pseudo-differential operators. The formula justifies the water-filling principle along time and frequency in terms of the time--continuous time-varying transfer function (the symbol).Comment: 5 pages, to be presented at ISIT 2011, minor typos corrected, references update