Advances in Raman and Surface-Enhanced Raman Spectroscopy: Instrumentation, Plasmonic Engineering and Biomolecular Sensing

Raman spectroscopy is a powerful technique for label-free molecular sensing and imaging in various fields. High molecular specificity, non-invasive sampling approach and the need for little or no sample preparation make Raman spectroscopy uniquely advantageous compared to other analytical techniques. However, Raman spectroscopy suffers from the intrinsic limitation of weak signal intensity. Therefore, time-sensitive studies such as diagnosis and clinical applications require improving the throughput of Raman instrumentation. Alternatively, surface-enhanced Raman scattering (SERS) improves the sensitivity by 10^6 to 10^14 times, making the weak Raman intensity no longer a limitation. Nevertheless, it is still a big challenge to engineer plasmonic substrates with high SERS enhancement, good uniformity and reproducibility. This thesis presents advances in: (1) Raman instrumentation towards high-throughput, environmental, biological and biomedical analysis; (2) SERS substrates with high enhancement factor (EF), uniformity and reproducibility; (3) biosensing applications including imaging of cell population and detection of biomolecules towards high time efficiency and sensitivity. In Raman instrumentation, we have built a high-throughput line-scan Raman microscope system and a novel parallel Raman microscope based on multiple-point active-illumination and wide-field hyperspectral data collection. Using the line-scan Raman microscope, we have performed chemical imaging of intact biological cells at the cell population level. We have also demonstrated more flexibility and throughput from the active-illumination Raman microscope in rapid chemical identification and screening of micro and nanoparticles and bacterial spores. Both Raman microscopes have been used to evaluate the large-area SERS uniformity of DC-sputtered gold nanoislands, a low-cost means to fabricate plasmonic substrates. In plasmonic engineering, we have introduced patterned nanoporous gold nanoparticles that feature 3-dimensional mesoporous network with pore size on the order of 10 nm throughput the sub-wavelength nanoparticles. We showed that the plasmonic resonance can be tuned by geometrical engineering of either the external nanoparticle size and shape or the nanoporous network. As an example, we have developed disk-shaped entities, also known as nanoporous gold disks (NPGD) with highly uniform and reproducible SERS EF exceeding 10^8. Label-free, multiplexed molecular sensing and imaging has been demonstrated on NPGD substrates. Using the line-scan Raman microscope and the NPGD substrates, we have successfully developed a label-free DNA hybridization sensor at the single-molecule level in microfluidics. We have observed discrete, individual DNA hybridization events by in situ monitoring the hybridization process using SERS. The advances and promising results presented in this thesis demonstrate potential impact in Raman/SERS imaging and sensing in environmental, biological and biomedical applications.Electrical and Computer Engineering, Department o

Novel haptic interface For viewing 3D images

In recent years there has been an explosion of devices and systems capable of displaying stereoscopic 3D images. While these systems provide an improved experience over traditional bidimensional displays they often fall short on user immersion. Usually these systems only improve depth perception by relying on the stereopsis phenomenon. We propose a system that improves the user experience and immersion by having a position dependent rendering of the scene and the ability to touch the scene. This system uses depth maps to represent the geometry of the scene. Depth maps can be easily obtained on the rendering process or can be derived from the binocular-stereo images by calculating their horizontal disparity. This geometry is then used as an input to be rendered in a 3D display, do the haptic rendering calculations and have a position depending render of the scene. The author presents two main contributions. First, since the haptic devices have a finite work space and limited resolution, we used what we call detail mapping algorithms. These algorithms compress geometry information contained in a depth map, by reducing the contrast among pixels, in such a way that it can be rendered into a limited resolution display medium without losing any detail. Second, the unique combination of a depth camera as a motion capturing system, a 3D display and haptic device to enhance user experience. While developing this system we put special attention on the cost and availability of the hardware. We decided to use only off-the-shelf, mass consumer oriented hardware so our experiments can be easily implemented and replicated. As an additional benefit the total cost of the hardware did not exceed the one thousand dollars mark making it affordable for many individuals and institutions

Publications of the Jet Propulsion Laboratory, 1977

This bibliography cites 900 externally distributed technical reports released during calendar year 1977, that resulted from scientific and engineering work performed, or managed, by the Jet Propulsion Laboratory. Report topics cover 81 subject areas related in some way to the various NASA programs. The publications are indexed by: (1) author, (2) subject, and (3) publication type and number. A descriptive entry appears under the name of each author of each publication; an abstract is included with the entry for the primary (first-listed) author

High-speed surface profilometry based on an adaptive microscope with axial chromatic encoding

An adaptive microscope with axial chromatic encoding is designed and developed, namely the AdaScope. With the ability to confocally address any locations within the measurement volume, the AdaScope provides the hardware foundation for a cascade measurement strategy to be developed, dramatically accelerating the speed of 3D confocal microscopy

Sustainable Agriculture and Advances of Remote Sensing (Volume 1)

Agriculture, as the main source of alimentation and the most important economic activity globally, is being affected by the impacts of climate change. To maintain and increase our global food system production, to reduce biodiversity loss and preserve our natural ecosystem, new practices and technologies are required. This book focuses on the latest advances in remote sensing technology and agricultural engineering leading to the sustainable agriculture practices. Earth observation data, in situ and proxy-remote sensing data are the main source of information for monitoring and analyzing agriculture activities. Particular attention is given to earth observation satellites and the Internet of Things for data collection, to multispectral and hyperspectral data analysis using machine learning and deep learning, to WebGIS and the Internet of Things for sharing and publishing the results, among others

Liquid Crystal on Silicon Devices: Modeling and Advanced Spatial Light Modulation Applications

Liquid Crystal on Silicon (LCoS) has become one of the most widespread technologies for spatial light modulation in optics and photonics applications. These reflective microdisplays are composed of a high-performance silicon complementary metal oxide semiconductor (CMOS) backplane, which controls the light-modulating properties of the liquid crystal layer. State-of-the-art LCoS microdisplays may exhibit a very small pixel pitch (below 4 ?m), a very large number of pixels (resolutions larger than 4K), and high fill factors (larger than 90%). They modulate illumination sources covering the UV, visible, and far IR. LCoS are used not only as displays but also as polarization, amplitude, and phase-only spatial light modulators, where they achieve full phase modulation. Due to their excellent modulating properties and high degree of flexibility, they are found in all sorts of spatial light modulation applications, such as in LCOS-based display systems for augmented and virtual reality, true holographic displays, digital holography, diffractive optical elements, superresolution optical systems, beam-steering devices, holographic optical traps, and quantum optical computing. In order to fulfil the requirements in this extensive range of applications, specific models and characterization techniques are proposed. These devices may exhibit a number of degradation effects such as interpixel cross-talk and fringing field, and time flicker, which may also depend on the analog or digital backplane of the corresponding LCoS device. The use of appropriate characterization and compensation techniques is then necessary

Research and technology, 1992

Selected research and technology activities at Ames Research Center, including the Moffett Field site and the Dryden Flight Research Facility, are summarized. These activities exemplify the Center's varied and productive research efforts for 1992

Earth Resources: A continuing bibliography with indexes, issue 17

This bibliography lists 775 reports, articles, and other documents introduced into the NASA scientific and technical information system between January 1 and March 31, 1978. Emphasis is placed on the use of remote sensing and geophysical instrumentation in spacecraft and aircraft to survey and inventory natural resources and urban areas. Subject matter is grouped according to agriculture and forestry, environmental changes and cultural resources, geodesy and cartography, geology and mineral resources, hydrology and water management, data processing and distribution systems, instrumentation and sensors, and economic analysis

3D multiple description coding for error resilience over wireless networks

This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Mobile communications has gained a growing interest from both customers and service providers alike in the last 1-2 decades. Visual information is used in many application domains such as remote health care, video –on demand, broadcasting, video surveillance etc. In order to enhance the visual effects of digital video content, the depth perception needs to be provided with the actual visual content. 3D video has earned a significant interest from the research community in recent years, due to the tremendous impact it leaves on viewers and its enhancement of the user’s quality of experience (QoE). In the near future, 3D video is likely to be used in most video applications, as it offers a greater sense of immersion and perceptual experience. When 3D video is compressed and transmitted over error prone channels, the associated packet loss leads to visual quality degradation. When a picture is lost or corrupted so severely that the concealment result is not acceptable, the receiver typically pauses video playback and waits for the next INTRA picture to resume decoding. Error propagation caused by employing predictive coding may degrade the video quality severely. There are several ways used to mitigate the effects of such transmission errors. One widely used technique in International Video Coding Standards is error resilience. The motivation behind this research work is that, existing schemes for 2D colour video compression such as MPEG, JPEG and H.263 cannot be applied to 3D video content. 3D video signals contain depth as well as colour information and are bandwidth demanding, as they require the transmission of multiple high-bandwidth 3D video streams. On the other hand, the capacity of wireless channels is limited and wireless links are prone to various types of errors caused by noise, interference, fading, handoff, error burst and network congestion. Given the maximum bit rate budget to represent the 3D scene, optimal bit-rate allocation between texture and depth information rendering distortion/losses should be minimised. To mitigate the effect of these errors on the perceptual 3D video quality, error resilience video coding needs to be investigated further to offer better quality of experience (QoE) to end users. This research work aims at enhancing the error resilience capability of compressed 3D video, when transmitted over mobile channels, using Multiple Description Coding (MDC) in order to improve better user’s quality of experience (QoE). Furthermore, this thesis examines the sensitivity of the human visual system (HVS) when employed to view 3D video scenes. The approach used in this study is to use subjective testing in order to rate people’s perception of 3D video under error free and error prone conditions through the use of a carefully designed bespoke questionnaire.Petroleum Technology Development Fund (PTDF