278,308 research outputs found
Comparing temporal behavior of fast objective video quality measures on a large-scale database
In many application scenarios, video quality assessment is required to be fast and reasonably accurate. The characterisation of objective algorithms by subjective assessment is well established but limited due to the small number of test samples. Verification using large-scale objectively annotated databases provides a complementary solution. In this contribution, three simple but fast measures are compared regarding their agreement on a large-scale database. In contrast to subjective experiments, not only sequence-wise but also framewise agreement can be analyzed. Insight is gained into the behavior of the measures with respect to 5952 different coding configurations of High Efficiency Video Coding (HEVC). Consistency within a video sequence is analyzed as well as across video sequences. The results show that the occurrence of discrepancies depends mostly on the configured coding structure and the source content. The detailed observations stimulate questions on the combined usage of several video quality measures for encoder optimization
Full Reference Objective Quality Assessment for Reconstructed Background Images
With an increased interest in applications that require a clean background
image, such as video surveillance, object tracking, street view imaging and
location-based services on web-based maps, multiple algorithms have been
developed to reconstruct a background image from cluttered scenes.
Traditionally, statistical measures and existing image quality techniques have
been applied for evaluating the quality of the reconstructed background images.
Though these quality assessment methods have been widely used in the past,
their performance in evaluating the perceived quality of the reconstructed
background image has not been verified. In this work, we discuss the
shortcomings in existing metrics and propose a full reference Reconstructed
Background image Quality Index (RBQI) that combines color and structural
information at multiple scales using a probability summation model to predict
the perceived quality in the reconstructed background image given a reference
image. To compare the performance of the proposed quality index with existing
image quality assessment measures, we construct two different datasets
consisting of reconstructed background images and corresponding subjective
scores. The quality assessment measures are evaluated by correlating their
objective scores with human subjective ratings. The correlation results show
that the proposed RBQI outperforms all the existing approaches. Additionally,
the constructed datasets and the corresponding subjective scores provide a
benchmark to evaluate the performance of future metrics that are developed to
evaluate the perceived quality of reconstructed background images.Comment: Associated source code: https://github.com/ashrotre/RBQI, Associated
Database:
https://drive.google.com/drive/folders/1bg8YRPIBcxpKIF9BIPisULPBPcA5x-Bk?usp=sharing
(Email for permissions at: ashrotreasuedu
Structural RNA Homology Search and Alignment Using Covariance Models
Functional RNA elements do not encode proteins, but rather function directly as RNAs. Many different types of RNAs play important roles in a wide range of cellular processes, including protein synthesis, gene regulation, protein transport, splicing, and more. Because important sequence and structural features tend to be evolutionarily conserved, one way to learn about functional RNAs is through comparative sequence analysis - by collecting and aligning examples of homologous RNAs and comparing them. Covariance models: CMs) are powerful computational tools for homology search and alignment that score both the conserved sequence and secondary structure of an RNA family. However, due to the high computational complexity of their search and alignment algorithms, searches against large databases and alignment of large RNAs like small subunit ribosomal RNA: SSU rRNA) are prohibitively slow. Large-scale alignment of SSU rRNA is of particular utility for environmental survey studies of microbial diversity which often use the rRNA as a phylogenetic marker of microorganisms. In this work, we improve CM methods by making them faster and more sensitive to remote homology. To accelerate searches, we introduce a query-dependent banding: QDB) technique that makes scoring sequences more efficient by restricting the possible lengths of structural elements based on their probability given the model. We combine QDB with a complementary filtering method that quickly prunes away database subsequences deemed unlikely to receive high CM scores based on sequence conservation alone. To increase search sensitivity, we apply two model parameterization strategies from protein homology search tools to CMs. As judged by our benchmark, these combined approaches yield about a 250-fold speedup and significant increase in search sensitivity compared with previous implementations. To accelerate alignment, we apply a method that uses a fast sequence-based alignment of a target sequence to determine constraints for the more expensive CM sequence- and structure-based alignment. This technique reduces the time required to align one SSU rRNA sequence from about 15 minutes to 1 second with a negligible effect on alignment accuracy. Collectively, these improvements make CMs more powerful and practical tools for RNA homology search and alignment
The Parallelism Motifs of Genomic Data Analysis
Genomic data sets are growing dramatically as the cost of sequencing
continues to decline and small sequencing devices become available. Enormous
community databases store and share this data with the research community, but
some of these genomic data analysis problems require large scale computational
platforms to meet both the memory and computational requirements. These
applications differ from scientific simulations that dominate the workload on
high end parallel systems today and place different requirements on programming
support, software libraries, and parallel architectural design. For example,
they involve irregular communication patterns such as asynchronous updates to
shared data structures. We consider several problems in high performance
genomics analysis, including alignment, profiling, clustering, and assembly for
both single genomes and metagenomes. We identify some of the common
computational patterns or motifs that help inform parallelization strategies
and compare our motifs to some of the established lists, arguing that at least
two key patterns, sorting and hashing, are missing
Likelihood-based inference of B-cell clonal families
The human immune system depends on a highly diverse collection of
antibody-making B cells. B cell receptor sequence diversity is generated by a
random recombination process called "rearrangement" forming progenitor B cells,
then a Darwinian process of lineage diversification and selection called
"affinity maturation." The resulting receptors can be sequenced in high
throughput for research and diagnostics. Such a collection of sequences
contains a mixture of various lineages, each of which may be quite numerous, or
may consist of only a single member. As a step to understanding the process and
result of this diversification, one may wish to reconstruct lineage membership,
i.e. to cluster sampled sequences according to which came from the same
rearrangement events. We call this clustering problem "clonal family
inference." In this paper we describe and validate a likelihood-based framework
for clonal family inference based on a multi-hidden Markov Model (multi-HMM)
framework for B cell receptor sequences. We describe an agglomerative algorithm
to find a maximum likelihood clustering, two approximate algorithms with
various trade-offs of speed versus accuracy, and a third, fast algorithm for
finding specific lineages. We show that under simulation these algorithms
greatly improve upon existing clonal family inference methods, and that they
also give significantly different clusters than previous methods when applied
to two real data sets
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