Correlated Multimodal Imaging in Life Sciences:Expanding the Biomedical Horizon

International audienceThe frontiers of bioimaging are currently being pushed toward the integration and correlation of several modalities to tackle biomedical research questions holistically and across multiple scales. Correlated Multimodal Imaging (CMI) gathers information about exactly the same specimen with two or more complementary modalities that-in combination-create a composite and complementary view of the sample (including insights into structure, function, dynamics and molecular composition). CMI allows to describe biomedical processes within their overall spatio-temporal context and gain a mechanistic understanding of cells, tissues, diseases or organisms by untangling their molecular mechanisms within their native environment. The two best-established CMI implementations for small animals and model organisms are hardware-fused platforms in preclinical imaging (Hybrid Imaging) and Correlated Light and Electron Microscopy (CLEM) in biological imaging. Although the merits of Preclinical Hybrid Imaging (PHI) and CLEM are well-established, both approaches would benefit from standardization of protocols, ontologies and data handling, and the development of optimized and advanced implementations. Specifically, CMI pipelines that aim at bridging preclinical and biological imaging beyond CLEM and PHI are rare but bear great potential to substantially advance both bioimaging and biomedical research. CMI faces three mai

Integration of MALDI FTICR MSI data with the open-source software Cytomine

editorial reviewedOur skin is constantly exposed to solar radiation, high oxygen levels, and environmental pollutants. Our study aims to target specific native (LipS) and oxidized phospholipids (oxLipS) that are known to be senescence-associated secretory phenotype (SASP) related (i.e., LysoPC, oxPAPC). The production of these oxidized species is obtained after exposure to UV light. Here, we employ MALDI FTICR mass spectrometry imaging (MSI) to visualize and identify lipid species of interest in an organotypic model system and to integrate this data with the open-source software Cytomine.COMULIS COST ACTIO

PITANJE IDENTITETA FRA BERNARDINA SPLIĆANINA, IZDAVAČA HRVATSKOG LEKCIONARA (1495.).

Proslavili smo 500. obljetnicu Lekcionara fra Bernardina Splićanina, prve hrvatske latiničke knjige tiskane u Veneciji 1495. Glavna proslava tog jubileja održana je od 1. do 4. listopada 1995. u samostanu sv. Frane u Splitu, uz sudjelovanje brojnih i uglednih predavatča koji su svestrano rasvijetlili taj izvanredan pothvat naše crkvene i kulturne povijesti.In occasione del 500. anniversario del primo libro in lingua croata in caratteri latini, Il lezionario di Fra Bernardino di Spalato, stampato a Venezia nel 1495. di nuovo è sottolineata l\u27importanza di questa opera importane per la cultura croata. Da tempo si disputa sull identita di questo celebre francescano, se era di famiglia osservante e apparteneva al convento di Pa/udi a Spalato, oppure di famiglia osservante del convento di S. Francesco di Spalato. L\u27autore dimostra che la tradizione di essere osservante ha più appoggio nei documenti. Tral\u27altro fra Bernardino quale il redattore del Lelzionario e da molti anni segnalato nel necrologio dei padri osservanti della provincia di S. Girolamo con la datta della morte, 27. luglio 1500. (oggi si sa che lo era esatamente nel 1499. e che portava il cognome Drivodilić). Inoltre nel convento di Paludi (Split) c\u27e un incunabulo con la nota: Hic liber concessus est fratri Bernardino di Spaleto. Questo frate in quell\u27 epoca e segnalato nei conventi osservanti di Zara, Lesina e Spalato. In fine l\u27autore documenta la tradizione diffusa nei conventi osservanti di tradurre testi liturgici latini in lingua vernacol

PITANJE IDENTITETA FRA BERNARDINA SPLIĆANINA, IZDAVAČA HRVATSKOG LEKCIONARA (1495.).

Proslavili smo 500. obljetnicu Lekcionara fra Bernardina Splićanina, prve hrvatske latiničke knjige tiskane u Veneciji 1495. Glavna proslava tog jubileja održana je od 1. do 4. listopada 1995. u samostanu sv. Frane u Splitu, uz sudjelovanje brojnih i uglednih predavatča koji su svestrano rasvijetlili taj izvanredan pothvat naše crkvene i kulturne povijesti.In occasione del 500. anniversario del primo libro in lingua croata in caratteri latini, Il lezionario di Fra Bernardino di Spalato, stampato a Venezia nel 1495. di nuovo è sottolineata l\u27importanza di questa opera importane per la cultura croata. Da tempo si disputa sull identita di questo celebre francescano, se era di famiglia osservante e apparteneva al convento di Pa/udi a Spalato, oppure di famiglia osservante del convento di S. Francesco di Spalato. L\u27autore dimostra che la tradizione di essere osservante ha più appoggio nei documenti. Tral\u27altro fra Bernardino quale il redattore del Lelzionario e da molti anni segnalato nel necrologio dei padri osservanti della provincia di S. Girolamo con la datta della morte, 27. luglio 1500. (oggi si sa che lo era esatamente nel 1499. e che portava il cognome Drivodilić). Inoltre nel convento di Paludi (Split) c\u27e un incunabulo con la nota: Hic liber concessus est fratri Bernardino di Spaleto. Questo frate in quell\u27 epoca e segnalato nei conventi osservanti di Zara, Lesina e Spalato. In fine l\u27autore documenta la tradizione diffusa nei conventi osservanti di tradurre testi liturgici latini in lingua vernacol

Characterisation of the antibiotic profile of Lysobacter capsici AZ78, an effective biological control agent of plant pathogenic microorganisms

Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium Lysobacter capsici AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against Pythium ultimum and Rhodococcus fascians in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by Lysobacter spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of L. capsici species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling Plasmopara viticola on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agricultur

Insights into Growth Factors in Liver Carcinogenesis and Regeneration: An Ongoing Debate on Minimizing Cancer Recurrence after Liver Resection

This research was funded by the Ministerio de Ciencia, Innovacion y Universidades (Project Grant RTI2018-095114-B-I00) Madrid, Spain; European Union (Fondos FEDER, "una manera de hacer Europa"); CERCA Program/Generalitat de Catalunya; the Secretaria d' Universitats I Recerca del Departament d' Economia I Coneixement (Project Grant 2017_SGR_551) Barcelona, Spain, by the COST action Programs CA17103 (DARTER), CA17112 (PRO-EURO-DILI-NET), CA17121 (COMULIS) and CA17126 (TUMIEE), and by the Consejo Nacional de Ciencia y Tecnologia (CONACYT), Fondo Sectorial de Investigacion para la Educacion (Project grant 257743), Mexico. Marc Mico-Carnero is the recipient of a fellowship from FCT (Fundacio Catalana de Trasplantament), Spain.Hepatocellular carcinoma has become a leading cause of cancer-associated mortality throughout the world, and is of great concern. Currently used chemotherapeutic drugs in the treatment of hepatocellular carcinoma lead to severe side effects, thus underscoring the need for further research to develop novel and safer therapies. Liver resection in cancer patients is routinely performed. After partial resection, liver regeneration is a perfectly calibrated response apparently sensed by the body’s required liver function. This process hinges on the effect of several growth factors, among other molecules. However, dysregulation of growth factor signals also leads to growth signaling autonomy and tumor progression, so control of growth factor expression may prevent tumor progression. This review describes the role of some of the main growth factors whose dysregulation promotes liver tumor progression, and are also key in regenerating the remaining liver following resection. We herein summarize and discuss studies focused on partial hepatectomy and liver carcinogenesis, referring to hepatocyte growth factor, insulin-like growth factor, and epidermal growth factor, as well as their suitability as targets in the treatment of hepatocellular carcinoma. Finally, and given that drugs remain one of the mainstay treatment options in liver carcinogenesis, we have reviewed the current pharmacological approaches approved for clinical use or research targeting these factors.Ministerio de Ciencia, Innovacion y Universidades Madrid, Spain RTI2018-095114-B-I00European Union (Fondos FEDER, "una manera de hacer Europa")General ElectricSecretaria d' Universitats I Recerca del Departament d' Economia I Coneixement Barcelona, Spain 2017_SGR_551COST action Programs CA17103 CA17112 CA17121 CA17126Consejo Nacional de Ciencia y Tecnologia (CONACyT)Fondo Sectorial de Investigacion para la Educacion, Mexico 257743FCT (Fundacio Catalana de Trasplantament), Spai

Marulić\u27s Six Friends

Concluding his renowned Latin epistle in verse, addressed to Franjo Božićević Natalis, the poet, who at that time was staying at Nečujam, sends his regards to some of his Split friends, inviting them all to his quiet cove on the island of Šolta: ... Ipsos nunc Thalia nostra salutat, Intima quos nobis iungit amicitia, Hyeronimum cythara clarum, gravitate Marinum, Ruris et agrorum cultibus Alvisium, Nicoleon risu facilem, sed ut ipse iubemus Ante salutetur Barbarus usque meus. Ille licet Musis et sanguine praestet avorum, Non tamen invitus carmina nostra legit. The author undertakes to find out the true identity of these personages, the only clue to it being their first names and the poet’s brief remarks on their characters. Hieronymus cythara clarus is most probably Jerolim Papalić (Hieronymus Papalis), son of Matija, who, according to the poet’s biographer F. Natalis, sang Marulić’s poems with great expressiveness, accompanied by the lyre (carmina sua ad lyram argutissimis modulis decantare solebat). Nicoleos risu facilis could refer to a certain Nikola Alberti, known for having sent his juvenile poems to Marulić and to whom the poet replied in a short epistle, praising his exceptional talent. The most difficult task was, however, to identify Marinus, whose characteristic, according to Marulić, was gravitas. The author suggests that this personage could be recognized in the figure of Marin Domić, bringing forth new evidence to complete the picture of that rather obscure contemporary of Marulić. Mentioned in archival docu-ments as ser Marinus Domitius, Domić was on terms of personal friends with the il-lustrious Toma Niger (Thomas Nigris), a close friend of Marulić. Toma’s younger brother Kristofor (Christoforus Nigris), to whom the poet dedicated an epitaph, prob-ably also belonged to Marulić’s humanist circle. Alvisius, good at cultivating fields, is probably Alviz Papalić (Alvisius Papalis), whom Marulić appointed to act as the executor of his will. Special greetings are sent to a certain Barbarus, distinguished, according to the poet, for his verse and his old stock. He is probably Ermolao Barbaro (Hermolaus Barbarus), who, at the beginning of his career, held the office of treasurer and castellan at Split and who came from an illustrious Venetian family conspicuous for propagating the ideas and habits of the Renaissance Humanism in the 15th and 16th centuries. The poet was undoubtedly well acquainted with the names and the prominence of Ermolao’s illustrious ancestors, since his library contained a work by Francesco Barbaro, entitled De re uxoria. Also, the poet probably kept in high regard the role that Ermolao, as the castellan of Split, played in the defence of the town against the Turks. The same grievous circumstances were the reason of Marulić’s invitation to his friends, who he wished to distract from concerns and sufferings brought about by Turkish bloody pillage, persecutions and violence, that were taking place in the surroundings of Split. Marulić’s epistle is an exhortation to his friends to forget all the miseries of the time and enjoy a fugitive moment of Renaissance convivality in his calm port

Bulloch Times

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