Improved Spectrum Usage with Multi-RF Channel Aggregation Technologies for the Next-Generation Terrestrial Broadcasting

[EN] Next-generation terrestrial broadcasting targets at enhancing spectral efficiency to overcome the challenges derived from the spectrum shortage as a result of the progressive allocation of frequencies - the so-called Digital Dividend - to satisfy the growing demands for wireless broadband capacity. Advances in both transmission standards and video coding are paramount to enable the progressive roll-out of high video quality services such as HDTV (High Definition Televison) or Ultra HDTV. The transition to the second generation European terrestrial standard DVB-T2 and the introduction of MPEG-4/AVC video coding already enables the transmission of 4-5 HDTV services per RF (Radio Frequency) channel. However, the impossibility to allocate higher bit-rate within the remaining spectrum could jeopardize the evolution of the DTT platforms in favour of other high-capacity systems such as the satellite or cable distribution platforms. Next steps are focused on the deployment of the recently released High Efficiency Video Coding (HEVC) standard, which provides more than 50% coding gain with respect to AVC, with the next-generation terrestrial standards. This could ensure the competitiveness of the DTT. This dissertation addresses the use of multi-RF channel aggregation technologies to increase the spectral efficiency of future DTT networks. The core of the Thesis are two technologies: Time Frequency Slicing (TFS) and Channel Bonding (CB). TFS and CB consist in the transmission of the data of a TV service across multiple RF channels instead of using a single channel. CB spreads data of a service over multiple classical RF channels (RF-Mux). TFS spreads the data by time-slicing (slot-by-slot) across multiple RF channels which are sequentially recovered at the receiver by frequency hopping. Transmissions using these features can benefit from capacity and coverage gains. The first one comes from a more efficient statistical multiplexing (StatMux) for Variable Bit Rate (VBR) services due to a StatMux pool over a higher number of services. Furthermore, CB allows increasing service data rate with the number of bonded RF channels and also advantages when combined with SVC (Scalable Video Coding). The coverage gain comes from the increased RF performance due to the reception of the data of a service from different RF channels rather that a single one that could be, eventually, degraded. Robustness against interferences is also improved since the received signal does not depend on a unique potentially interfered RF channel. TFS was firstly introduced as an informative annex in DVB-T2 (not normative) and adopted in DVB-NGH (Next Generation Handheld). TFS and CB are proposed for inclusion in ATSC 3.0. However, they have never been implemented. The investigations carried out in this dissertation employ an information-theoretical approach to obtain their upper bounds, physical layer simulations to evaluate the performance in real systems and the analysis of field measurements that approach realistic conditions of the network deployments. The analysis report coverage gains about 4-5 dB with 4 RF channels and high capacity gains already with 2 RF channels. This dissertation also focuses on implementation aspects. Channel bonding receivers require one tuner per bonded RF channel. The implementation of TFS with a single tuner demands the fulfilment of several timing requirements. However, the use of just two tuners would still allow for a good performance with a cost-effective implementation by the reuse of existing chipsets or the sharing of existing architectures with dual tuner operation such as MIMO (Multiple Input Multiple Output).[ES] La televisión digital terrestre (TDT) de última generación está orientada a una necesaria mejora de la eficiencia espectral con el fin de abordar los desafíos derivados de la escasez de espectro como resultado de la progresiva asignación de frecuencias - el llamado Dividendo Digital - para satisfacer la creciente demanda de capacidad para la banda ancha inalámbrica. Los avances tanto en los estándares de transmisión como de codificación de vídeo son de suma importancia para la progresiva puesta en marcha de servicios de alta calidad como la televisión de Ultra AD (Alta Definición). La transición al estándar europeo de segunda generación DVB-T2 y la introducción de la codificación de vídeo MPEG-4 / AVC ya permite la transmisión de 4-5 servicios de televisión de AD por canal RF (Radiofrecuencia). Sin embargo, la imposibilidad de asignar una mayor tasa de bit sobre el espectro restante podría poner en peligro la evolución de las plataformas de TDT en favor de otros sistemas de alta capacidad tales como el satélite o las distribuidoras de cable. El siguiente paso se centra en el despliegue del reciente estándar HEVC (High Efficiency Video Coding), que ofrece un 50% de ganancia de codificación con respecto a AVC, junto con los estándares terrestres de próxima generación, lo que podría garantizar la competitividad de la TDT en un futuro cercano. Esta tesis aborda el uso de tecnologías de agregación de canales RF que permitan incrementar la eficiencia espectral de las futuras redes. La tesis se centra en torno a dos tecnologías: Time Frequency Slicing (TFS) y Channel Bonding (CB). TFS y CB consisten en la transmisión de los datos de un servicio de televisión a través de múltiples canales RF en lugar de utilizar un solo canal. CB difunde los datos de un servicio a través de varios canales RF convencionales formando un RF-Mux. TFS difunde los datos a través de ranuras temporales en diferentes canales RF. Los datos son recuperados de forma secuencial en el receptor mediante saltos en frecuencia. La implementación de estas técnicas permite obtener ganancias en capacidad y cobertura. La primera de ellas proviene de una multiplexación estadística (StatMux) de servicios de tasa variable (VBR) más eficiente. Además, CB permite aumentar la tasa de pico de un servicio de forma proporcional al número de canales así como ventajas al combinarla con codificación de vídeo escalable. La ganancia en cobertura proviene de un mejor rendimiento RF debido a la recepción de los datos de un servicio desde diferentes canales en lugar uno sólo que podría estar degradado. Del mismo modo, es posible obtener una mayor robustez frente a interferencias ya que la recepción o no de un servicio no depende de si el canal que lo alberga está o no interferido. TFS fue introducido en primer lugar como un anexo informativo en DVB-T2 (no normativo) y posteriormente fue adoptado en DVB-NGH (Next Generation Handheld). TFS y CB han sido propuestos para su inclusión en ATSC 3.0. Aún así, nunca han sido implementados. Las investigaciones llevadas a cabo en esta Tesis emplean diversos enfoques basados en teoría de la información para obtener los límites de ganancia, en simulaciones de capa física para evaluar el rendimiento en sistemas reales y en el análisis de medidas de campo. Estos estudios reportan ganancias en cobertura en torno a 4-5 dB con 4 canales e importantes ganancias en capacidad aún con sólo 2 canales RF. Esta tesis también se centra en los aspectos de implementación. Los receptores para CB requieren un sintonizador por canal RF agregado. La implementación de TFS con un solo sintonizador exige el cumplimiento de varios requisito temporales. Sin embargo, el uso de dos sintonizadores permitiría un buen rendimiento con una implementación más rentable con la reutilización de los actuales chips o su introducción junto con las arquitecturas existentes que operan con un doble sintonizador tales como[CA] La televisió digital terrestre (TDT) d'última generació està orientada a una necessària millora de l'eficiència espectral a fi d'abordar els desafiaments derivats de l'escassetat d'espectre com a resultat de la progressiva assignació de freqüències - l'anomenat Dividend Digital - per a satisfer la creixent demanda de capacitat per a la banda ampla sense fil. Els avanços tant en els estàndards de transmissió com de codificació de vídeo són de la màxima importància per a la progressiva posada en marxa de serveis d'alta qualitat com la televisió d'Ultra AD (Alta Definició). La transició a l'estàndard europeu de segona generació DVB-T2 i la introducció de la codificació de vídeo MPEG-4/AVC ja permet la transmissió de 4-5 serveis de televisió d'AD per canal RF (Radiofreqüència). No obstant això, la impossibilitat d'assignar una major taxa de bit sobre l'espectre restant podria posar en perill l'evolució de les plataformes de TDT en favor d'altres sistemes d'alta capacitat com ara el satèl·lit o les distribuïdores de cable. El següent pas se centra en el desplegament del recent estàndard HEVC (High Efficiency Vídeo Coding), que oferix un 50% de guany de codificació respecte a AVC, junt amb els estàndards terrestres de pròxima generació, la qual cosa podria garantir la competitivitat de la TDT en un futur pròxim. Aquesta tesi aborda l'ús de tecnologies d'agregació de canals RF que permeten incrementar l'eficiència espectral de les futures xarxes. La tesi se centra entorn de dues tecnologies: Time Frequency Slicing (TFS) i Channel Bonding (CB). TFS i CB consistixen en la transmissió de les dades d'un servei de televisió a través de múltiples canals RF en compte d'utilitzar un sol canal. CB difon les dades d'un servei a través d'uns quants canals RF convencionals formant un RF-Mux. TFS difon les dades a través de ranures temporals en diferents canals RF. Les dades són recuperades de forma seqüencial en el receptor per mitjà de salts en freqüència. La implementació d'aquestes tècniques permet obtindre guanys en capacitat i cobertura. La primera d'elles prové d'una multiplexació estadística (StatMux) de serveis de taxa variable (VBR) més eficient. A més, CB permet augmentar la taxa de pic d'un servei de forma proporcional al nombre de canals així com avantatges al combinar-la amb codificació de vídeo escalable. El guany en cobertura prové d'un millor rendiment RF a causa de la recepció de les dades d'un servei des de diferents canals en lloc de només un que podria estar degradat. De la mateixa manera, és possible obtindre una major robustesa enfront d'interferències ja que la recepció o no d'un servei no depén de si el canal que l'allotja està o no interferit. TFS va ser introduït en primer lloc com un annex informatiu en DVB-T2 (no normatiu) i posteriorment va ser adoptat en DVB-NGH (Next Generation Handheld). TFS i CB han sigut proposades per a la seva inclusió en ATSC 3.0. Encara així, mai han sigut implementades. Les investigacions dutes a terme en esta Tesi empren diverses vessants basades en teoria de la informació per a obtindre els límits de guany, en simulacions de capa física per a avaluar el rendiment en sistemes reals i en l'anàlisi de mesures de camp. Aquestos estudis reporten guanys en cobertura entorn als 4-5 dB amb 4 canals i importants guanys en capacitat encara amb només 2 canals RF. Esta tesi també se centra en els aspectes d'implementació. Els receptors per a CB requerixen un sintonitzador per canal RF agregat. La implementació de TFS amb un sol sintonitzador exigix el compliment de diversos requisit temporals. No obstant això, l'ús de dos sintonitzadors permetria un bon rendiment amb una implementació més rendible amb la reutilització dels actuals xips o la seua introducció junt amb les arquitectures existents que operen amb un doble sintonitzador com ara MIMO (Multiple Input Multiple Output).Giménez Gandia, JJ. (2015). Improved Spectrum Usage with Multi-RF Channel Aggregation Technologies for the Next-Generation Terrestrial Broadcasting [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/52520TESI

Human central nervous system (CNS) ApoE isoforms are increased by age, differentially altered by amyloidosis, and relative amounts reversed in the CNS compared with plasma

The risk of Alzheimer's disease (AD) is highly dependent on apolipoprotein-E (apoE) genotype. The reasons for apoE isoform-selective risk are uncertain; however, both the amounts and structure of human apoE isoforms have been hypothesized to lead to amyloidosis increasing the risk for AD. To address the hypothesis that amounts of apoE isoforms are different in the human CNS, we developed a novel isoform-specific method to accurately quantify apoE isoforms in clinically relevant samples. The method utilizes an antibody-free enrichment step and isotope-labeled physiologically relevant lipoprotein particle standards produced by immortalized astrocytes. We applied this method to a cohort of well characterized clinical samples and observed the following findings. The apoE isoform amounts are not different in cerebrospinal fluid (CSF) from young normal controls, suggesting that the amount of apoE isoforms is not the reason for risk of amyloidosis prior to the onset of advanced age. We did, however, observe an age-related increase in both apoE isoforms. In contrast to normal aging, the presence of amyloid increased apoE3, whereas apoE4 was unchanged or decreased. Importantly, for heterozygotes, the apoE4/apoE3 isoform ratio was increased in the CNS, although the reverse was true in the periphery. Finally, CSF apoE levels, but not plasma apoE levels, correlated with CSF β-amyloid levels. Collectively, these findings support the hypothesis that CNS and peripheral apoE are separate pools and differentially regulated. Furthermore, these results suggest that apoE mechanisms for the risk of amyloidosis and AD are related to an interaction between apoE, aging, and the amount of amyloid burden

Analytical Methods to Support Design and Optimization of Protein Drug Conjugate: Focusing on Haptoglobin-hemoglobin Complex as a Drug Carrier

Acquired immunodeficiency syndrome (AIDS) remains one of the most serious public health challenges and a significant cause of mortality for certain populations. Despite the large number of antiretrovirals (mostly nucleotide and nucleoside analogs) developed in the past two decades, the inability of small molecule therapeutics to target HIV reservoirs directly creates a significant obstacle to their effective utilization. Indeed, achieving the desired therapeutic effect in the absence of the effective targeted delivery must rely on dosage escalation, which frequently causes severe toxicity. This problem may be solved by conjugation of antiretroviral agents to endogenous proteins (e.g., hemoglobin haptoglobin complex) that are specifically recognized by HIV reservoirs (such as macrophages) for internalization and catabolism. However, conjugation of a large class of antiretroviral agents (acyclic nucleoside phosphonates, such as adefovir) to a protein is challenging due to the rapid decay (including hydrolysis and dimerization) of the activated form of the drug (adefovir phosphonoimidazolide) during transition (ether precipitation) from organic phase to aqueous phase. This work introduces a novel synthetic strategy which overcomes the instability of the activated form of adefovir by emulating the first step of its metabolic pathway (phosphorylation), making it highly reactive towards primary amine groups of proteins. The effective conjugation of phosphorylated form of adefovir to protein via an imidazolide based intermediate was demonstrated using lysozyme as a model carrier protein. Mass spectrometry (MS) based analytical methods were used to support design and optimization of all those conjugations. Further optimization of adefovir’s conjugation with hemoglobin (Hb), a drug carrier which targets macrophage via haptoglobin (Hp)-CD163 mediated heme scavenging system, was pursued using another novel linker, phosphonoacetate, which allows reactions to be performed at neutral pH with a satisfactory yield. Successful loading of adefovir to Hp, the obligatory partner of Hb for targeted drug delivery, via Hb·Hp binding was demonstrated by MS. Lastly, a new strategy was developed for detecting and quantitating exogenous Hp·Hb complex with high sensitivity in complex biological samples using gallium as a tracer of this protein and inductively coupled plasma mass spectrometry (ICP MS) as a method of detection

Energy Efficient Small Cell Planning For High Capacity Wireless Networks

This thesis presents a new strategy to densify Small Cells (i.e., add more low powered base stations within macro networks) and enhance the coverage and capacity of Heterogeneous Networks. This is accomplished by designing Micro Cell for outdoor applications, Pico and Femtocell for indoor applications. It is shown that, there exists a free space propagation medium in all propagation environments due to Fresnel zones, and the path loss slope within this zone is similar to free space propagation medium. This forms the basis of our development of the present work. The salient feature of the proposed work has two main considerations (a) The cell radius of Small Cells must be within the first Fresnel zone break point, and (b) The minimum inter-cell distance must be greater than twice of Small Cell radius. The proposed network is simulated in real a radio network simulator called ATOLL. The simulation results showed that densify Small Cells not only enhanced the capacity and coverage of Heterogeneous Networks but also improved the carrier to interference ratio significantly. Since the proposed work allows UE (user equipment) to have Line of Sight (LOS) communication with the serving cell, and UE can have higher uplink (UL) signal to interference plus noise ratio (SINR) that will further allow UE to reduce its transmission power, which will consequently lead to a longer battery life for the UE and reduce the interference in the system

Contribution to the optimization of 4G mobile communications by means of advanced carrier aggregation strategies

Mobile broadband subscriptions and data traffic have increasingly grown in the past years with the deployment of the 3G and 4G technologies and the massive use of mobile devices. In this sense, LTE-A has been presented as the next step in wireless communications where higher data rates are targeted and fully packet switched services are held. The ultimate goal of 4G and the forthcoming 5G technology is to increase the Quality of Experience (QoE) of users. In this context, several challenges open up to face the increased bandwidth demands in both uplink (UL) and downlink (DL). To this end, LTE-A has proposed the use of Carrier Aggregation (CA) which allows the simultaneous data transmission in separate fragments of spectrum. The improvements brought by CA in the DL can be almost straightforward appreciable, since the evolved Node B (eNB) is in charge of transmissions, and power availability is not typically an issue. Conversely, the UL presents many open challenges to introduce aggregated transmissions, since it relies on the user terminal for transmission procedures. Lower transmission power and increased interference variability turn the UL more complex than the DL. For this reason, this Ph.D. thesis provides a contribution to the field of CA for UL mobile systems. The novelties here presented address the main limitations the UL encounters when introducing CA; new methods and strategies are proposed with the final aim of enhancing the UL communications with the use of increased bandwidth transmissions, and reducing the unbalanced data rate between the UL and DL. Throughout an exhaustive literature review, the main research opportunities to successfully implement CA in the UL were identified. In particular, three main blocks can be recognized. First, the need for introducing intelligent Radio Resource Management procedures that provide the user with increased QoE, specially in the cell edge, where users are more likely to be power limited, and CA is typically discarded. Consequently, the first part of this dissertation places emphasis on topics related to scheduling and the power limitations to face the increased bandwidth. In this sense, mechanisms that tackle the throughput improvement are proposed and scheduling schemes that specifically assess the gain or deterioration of CA are designed. Indeed, these strategies strongly rely on an accurate Channel State Information (CSI); it is of utmost importance to possess precise CSI to effectively support these assessments. In this line, the second part deals with the imperfect CSI where the efficient use of reference signals provides a high value. Channel prediction techniques have been proposed with the use of the splines method. However, the increased variability of interferences and the high delay in measurements still impairs the CSI accuracy. In this manner, interference management methods are introduced to support the CSI acquisition process. Finally, since CA constitutes the most transverse topic of the new features added to the 4G standard, the last block of research focuses on the opportunities that emerge with the use of CA in the context of heterogeneous networks, and new system designs are addressed. It is proposed to use dual connectivity in the form of decoupled uplink and downlink connections in a CA context, where aggregated carriers may have different coverage footprints. An analysis of two different cell association cases that arise has been driven. Stochastic geometry is used to study the system analytically, propagation conditions in the different tiers and frequencies are considered and the different association cases are compared to a classical downlink received power association rule. Conclusions show that decoupling the uplink provides the system with outstanding gains, however, being connected to the cell that receives the highest received power may not always be profitable, since issues like interferences or load conditions shall be also considered.El número de usuarios móviles y el tráfico de datos generado han aumentado en los últimos años con el despliegue de redes 3G y 4G y el uso masivo de dispositivos móviles. De este modo, LTE-A surge como el siguiente escalón de las comunicaciones móviles, dónde se apunta a mayores velocidades de transmisión y los servicios se basan en la conmutación de paquetes. El objetivo principal de las redes 4G y de la inminente red 5G es mejorar la experiencia del usuario. En este contexto, se presentan nuevos retos para hacer frente a las demandas de incrementar el ancho de banda en ambos enlaces: ascendente (UL) y descendente (DL). Por ello, LTE-A propone el uso de portadoras agregadas (Carrier Aggregation (CA)), tecnología que permite la transmisión simultánea en dos fragmentos del espectro. Las mejoras que aporta CA en el DL son casi inmediatas dado que las transmisiones corren a cargo de la base, la cual no sufre la falta de potencia. Al contrario, el UL presenta más retos para introducir CA, ya que es el terminal quién se encarga de la transmisión. La baja disponibilidad de potencia y la alta variabilidad de la interferencia lo convierten en un entorno mucho más complejo. Por ello, esta disertación presenta una contribución al campo de CA en el UL de comunicaciones móviles. Las novedades presentadas tratan las principales limitaciones para incorporar CA; se proponen nuevos métodos y estrategias con el objetivo de mejorar las comunicaciones en el UL mediante el uso de CA; todo ello, para reducir el desajuste que existe entre la velocidad de transmisión del UL y DL. Mediante una extensa revisión de la literatura, se han detectado las principales líneas de investigación y potenciales mejoras para incorporar CA exitosamente. Se han identificado tres grandes bloques de investigación. Primero, la necesidad de introducir estrategias de gestión de recursos inteligentes, que proporcionen al usuario una mejora de la experiencia, especialmente en el límite de la celda. Es allí donde los usuarios tienen una mayor probabilidad de estar limitados en potencia, razón por la que se les aparta de CA. Consecuentemente, la primera parte de esta tesis pone énfasis en la asignación de recursos y las limitaciones en potencia por parte del usuario para hacer frente a un incremento del ancho de banda. Se proponen mecanismos que mejoran la velocidad de transmisión evaluando las ganancias o pérdidas de incorporar CA a la transmisión. Para apoyar el funcionamiento de estas estrategias de asignación, y asegurar su máximo rendimiento, es necesario un método que proporcione un conocimiento preciso y fidedigno del estado del canal (Channel State Information (CSI)). De este modo, la segunda parte de la investigación lidia con el CSI, donde el uso eficiente de las señales de referencia es de gran importancia. Se proponen técnicas de predicción de señal mediante el uso de Splines; sin embargo, la alta variabilidad de las interferencias y el gran retardo entre dos muestras de CSI perjudican la precisión. Por ello, se introducen métodos de gestión de interferencias que apoyan el proceso de adquisición del CSI. Finalmente, dado que CA es una de las funciones más transversales de las introducidas por el estándar 4G, la última parte de investigación se centra en las oportunidades que surgen con su uso en las redes heterogéneas. Se propone el uso de la conectividad dual, desacoplando el UL del DL junto con CA, donde el área de cobertura de las portadoras puede ser diferente. Se analizan dos escenarios de asociación posibles. Con el uso de geometría estocástica se estudia analíticamente el sistema, considerando diferentes condiciones de propagación en los distintos tipos de celda y frecuencias; los escenarios de asociación se comparan a uno tradicional, en el cual los usuarios se asocian en función de la potencia recibida de las bases. Las conclusiones destacan que el desacoplo aporta mejoras en el UL. Sin embargo, temas como interferencias o carga deben también considera

Oligopolies in private spectrum commons: analysis and regulatory implications

In an effort to make more spectrum available, recent initiatives by the FCC let mobile providers offer spot service of their licensed spectrum to secondary users, hence paving the way to dynamic secondary spectrum markets. This dissertation investigates secondary spectrum markets under different regulatory regimes by identifying profitability conditions and possible competitive outcomes in an oligopoly model. We consider pricing in a market where multiple providers compete for secondary demand. First, we analyze the market outcomes when providers adopt a coordinated access policy, where, besides pricing, a provider can elect to apply admission control on secondary users based on the state of its network. We next consider a competition when providers implement an uncoordinated access policy (i.e., no admission control). Through our analysis, we identify profitability conditions and fundamental price thresholds, including break-even and market sharing prices. We prove that regardless of the specific form of the secondary demand function, competition under coordinated access always leads to a price war outcome. In contrast, under uncoordinated access, market sharing becomes a viable market outcome if the intervals of prices for which the providers are willing to share the market overlap. We then turn our attention to how a network provider use carrier (spectrum) aggregation in order to lower its break-even price and gain an edge over its competition. To this end, we determine the optimal (minimum) level of carrier aggregation that a smaller provider needs. Under a quality-driven (QD) regime, we establish an efficient way of numerically calculating the optimal carrier aggregation and derive scaling laws. We extend the results to delay-related metrics and show their applications to profitable pricing in secondary spectrum markets. Finally, we consider the problem of profitability over a spatial topology, where identifying system behavior suffers from the curse of dimensionality. Hence, we propose an approximation model that captures system behavior to the first-order and provide an expression to calculate the break-even price at each network location and provide simulation results for accuracy comparison. All of our results hold for general forms of demand, thus avoid restricting assumptions of customer preferences and the valuation of the spectrum

Applications of Paper Microfluidic Systems in the Field Detection of Drugs of Abuse

Over the years, colorimetric reagents and immunology have been widely used in screening tests for illicit drugs; however, the test kits are not always convenient for field use and often require the user to mix and develop a specific set of reagents. In our project, we have been working on alternative platforms based on paper microfluidic devices (uPADs) for field testing. These devices utilize wax channels printed on paper to direct the analyte towards a specific set of chemical reagents. Using the procedure, we have developed a six-channel chip that adapts known colorimetric reagents targeting cocaine, opiates, amphetamines and ketamine for multiplex detection. For more sensitive and specific determinations than the colorimetric reagents, we have also developed a paper device that utilizes the interaction between gold nanoparticles and drug specific aptamers. The µPADs using colorimetric reagent are designed as a six-channel multiplexed system. Sequences of different reagents applied to each channel to produce a series of reactions and the color changes appear at the end of each channel. The entire process takes less than five minutes. The adjusted reagents produce specific color changes for seized drugs on the paper microfluidic devices. Procedures have been developed for the detection of cocaine, ketamine, codeine, ephedrine, morphine, amphetamine, methamphetamine, and MDMA. These devices have been tested for sensitivity, specificity and stability against a variety of potential interferences and test conditions. Gold nanoparticles (AuNPs)/ aptamers µPADs were developed to detect cocaine. The presence of cocaine cause the binding with aptamers, and the gold nanoparitcles produced a salt-indicated aggregations and gave a color change of AuNPs from red to black. The absence of cocaine allowed the aptamers freely to bind gold nanoparticles, and no color change occured. The device had a preliminary validation of sensitvity and specificity against a variety of potential interferences. The use of paper microfluidic devices permits the development of rapid, inexpensive and easily operated tests for drug samples in the field. They present a safe and convenient presumptive tool that can be used in the field

Polymeric versus lipid nanoparticles: comparative study of nanoparticulate systems as indomethacin carriers

Encapsulation of nonsteroidal or non-steroidal anti-inflammatory drugs (NSAID) in nanocarrier systems aims to enhance bioavailability and to decrease toxicity of these drugs and thus improve the efficacy of treatments. With this aim two types of nanoparticles were prepared and compared: lipid nanoparticles, made of cetyl palmitate and Miglyol 812 which were uncoated or coated with chitosan; or polymeric nanoparticles, made of poly (DL-lactic-co-glycolic acid) (PLGA) for which different emulsion stabilizers were also tested (poly (vinyl alcohol) (PVA), and Pluronic F68). Nanoparticles were characterized for drug content and for particle size, charge and morphology. The lipid matrix was analyzed regarding its crystallinity by differential scanning calorimetry (DSC). The size of the nanoparticles was measured by dynamic light scattering (DLS) which indicated a unimodal particle size distribution in all systems. Nanoparticles’ stability was confirmed by their highly negative surface charge in the case of polymeric and uncoated lipid nanoparticles, as analyzed by zeta potential measurements using electrophoretic light scattering (ELS). Lipid chitosan coated nanoparticles have also shown to be stable presenting highly positive surface charge. Results have further demonstrated that indomethacin is highly encapsulated regardless the type of particles. Morphological analysis by scanning electron microscopy has shown that the nanoparticles were smooth and spherical. The results gathered within the current study point to the conclusion that the proposed formulations provide nanoparticles of satisfactory quality to encapsulate indomethacin, which might be used to improve bioavailability of other NSAID in the treatment of inflammation.This work was supported by FEDER through POFC – COMPETE and by national funds from FCT through the projects PEst-C/FIS/UI0607/2013 (CFUM). Marlene Lucio holds a position of Researcher FCT with the reference IF/00498/2012. We acknowledge NanoDelivery-I&D em Bionanotecnologia, Lda for access to their equipment.info:eu-repo/semantics/publishedVersio

Cooperative Spectrum Sharing of Cellular LTE-Advanced and Broadcast DVB-T2 Systems

The allocation of parts of the Ultra High Frequency (UHF) band to International Mobile Telecommunications (IMT) technologies on a co-primary basis with terrestrial broadcasting technologies has been the major change in worldwide spectrum allocation in recent years. Nowadays, thanks to the Second Generation for Terrestrial Digital Video Broadcasting (DVB-T2) and the Long Term Evolution Advanced (LTE-A) technologies a new model of cooperation between cellular and broadcasting systems arises, where the cellular network can use of broadcast spectrum using time multiplexing. This paper proposes the cooperative spectrum sharing of DTT spectrum between DVB-T2 systems and LTE-A cellular networks by means of the use of DVB-T2 FEF for LTE-A analyzing the potential benefit.Calabuig Gaspar, J.; Monserrat Del Río, JF.; Gómez Barquero, D.; Cardona Marcet, N. (2013). Cooperative Spectrum Sharing of Cellular LTE-Advanced and Broadcast DVB-T2 Systems. Transaction on IoT and Cloud Computing. 1(1):1-16. http://hdl.handle.net/10251/46750S1161