Digital twins: a survey on enabling technologies, challenges, trends and future prospects

Digital Twin (DT) is an emerging technology surrounded by many promises, and potentials to reshape the future of industries and society overall. A DT is a system-of-systems which goes far beyond the traditional computer-based simulations and analysis. It is a replication of all the elements, processes, dynamics, and firmware of a physical system into a digital counterpart. The two systems (physical and digital) exist side by side, sharing all the inputs and operations using real-time data communications and information transfer. With the incorporation of Internet of Things (IoT), Artificial Intelligence (AI), 3D models, next generation mobile communications (5G/6G), Augmented Reality (AR), Virtual Reality (VR), distributed computing, Transfer Learning (TL), and electronic sensors, the digital/virtual counterpart of the real-world system is able to provide seamless monitoring, analysis, evaluation and predictions. The DT offers a platform for the testing and analysing of complex systems, which would be impossible in traditional simulations and modular evaluations. However, the development of this technology faces many challenges including the complexities in effective communication and data accumulation, data unavailability to train Machine Learning (ML) models, lack of processing power to support high fidelity twins, the high need for interdisciplinary collaboration, and the absence of standardized development methodologies and validation measures. Being in the early stages of development, DTs lack sufficient documentation. In this context, this survey paper aims to cover the important aspects in realization of the technology. The key enabling technologies, challenges and prospects of DTs are highlighted. The paper provides a deep insight into the technology, lists design goals and objectives, highlights design challenges and limitations across industries, discusses research and commercial developments, provides its applications and use cases, offers case studies in industry, infrastructure and healthcare, lists main service providers and stakeholders, and covers developments to date, as well as viable research dimensions for future developments in DTs

Molecular phylogeny of horseshoe crab using mitochondrial Cox1 gene as a benchmark sequence

An effort to assess the utility of 650 bp Cytochrome C oxidase subunit I (DNA barcode) gene in delineating the members horseshoe crabs (Family: xiphosura) with closely related sister taxa was made. A total of 33 sequences were extracted from National Center for Biotechnological Information (NCBI) which include horseshoe crabs, beetles, common crabs and scorpion sequences. Constructed phylogram showed beetles are closely related with horseshoe crabs than common crabs. Scorpion spp were distantly related to xiphosurans. Phylogram and observed genetic distance (GD) date were also revealed that Limulus polyphemus was closely related with Tachypleus tridentatus than with T.gigas. Carcinoscorpius rotundicauda was distantly related with L.polyphemus. The observed mean Genetic Distance (GD) value was higher in 3rd codon position in all the selected group of organisms. Among the horseshoe crabs high GC content was observed in L.polyphemus (38.32%) and lowest was observed in T.tridentatus (32.35%). We conclude that COI sequencing (barcoding) could be used in identifying and delineating evolutionary relatedness with closely related specie

Crab and cockle shells as heterogeneous catalysts in the production of biodiesel

In the present study, the waste crab and cockle shells were utilized as source of calcium oxide to transesterify palm olein into methyl esters (biodiesel). Characterization results revealed that the main component of the shells are calcium carbonate which transformed into calcium oxide upon activated above 700 °C for 2 h. Parametric studies have been investigated and optimal conditions were found to be catalyst amount, 5 wt.% and methanol/oil mass ratio, 0.5:1. The waste catalysts perform equally well as laboratory CaO, thus creating another low-cost catalyst source for producing biodiesel. Reusability results confirmed that the prepared catalyst is able to be reemployed up to five times. Statistical analysis has been performed using a Central Composite Design to evaluate the contribution and performance of the parameters on biodiesel purity

Smart Urban Water Networks

This book presents the paper form of the Special Issue (SI) on Smart Urban Water Networks. The number and topics of the papers in the SI confirm the growing interest of operators and researchers for the new paradigm of smart networks, as part of the more general smart city. The SI showed that digital information and communication technology (ICT), with the implementation of smart meters and other digital devices, can significantly improve the modelling and the management of urban water networks, contributing to a radical transformation of the traditional paradigm of water utilities. The paper collection in this SI includes different crucial topics such as the reliability, resilience, and performance of water networks, innovative demand management, and the novel challenge of real-time control and operation, along with their implications for cyber-security. The SI collected fourteen papers that provide a wide perspective of solutions, trends, and challenges in the contest of smart urban water networks. Some solutions have already been implemented in pilot sites (i.e., for water network partitioning, cyber-security, and water demand disaggregation and forecasting), while further investigations are required for other methods, e.g., the data-driven approaches for real time control. In all cases, a new deal between academia, industry, and governments must be embraced to start the new era of smart urban water systems

Optimisation, Optimal Control and Nonlinear Dynamics in Electrical Power, Energy Storage and Renewable Energy Systems

The electrical power system is undergoing a revolution enabled by advances in telecommunications, computer hardware and software, measurement, metering systems, IoT, and power electronics. Furthermore, the increasing integration of intermittent renewable energy sources, energy storage devices, and electric vehicles and the drive for energy efficiency have pushed power systems to modernise and adopt new technologies. The resulting smart grid is characterised, in part, by a bi-directional flow of energy and information. The evolution of the power grid, as well as its interconnection with energy storage systems and renewable energy sources, has created new opportunities for optimising not only their techno-economic aspects at the planning stages but also their control and operation. However, new challenges emerge in the optimization of these systems due to their complexity and nonlinear dynamic behaviour as well as the uncertainties involved.This volume is a selection of 20 papers carefully made by the editors from the MDPI topic “Optimisation, Optimal Control and Nonlinear Dynamics in Electrical Power, Energy Storage and Renewable Energy Systems”, which was closed in April 2022. The selected papers address the above challenges and exemplify the significant benefits that optimisation and nonlinear control techniques can bring to modern power and energy systems

Factories of the Future

Engineering; Industrial engineering; Production engineerin

Comparative secretomics and functional analysis of effectors utilized by the Microbotryum genus of anther-smut fungal pathogens, and their role in host-specificity.

Understanding how pathogens evolve in response to changes in their host is paramount to combating the spread of emergent strains of disease. This is particularly true for plant pathogens that cause billions of dollars of damages to crops globally, every year. Understanding the molecular interactions between pathogens and their hosts therefore sheds light on the coevolutionary arms race that can result in host-specificity and host-shifts in plant pathogens. This research approaches the question of how fungal pathogens interact with their plant hosts utilizing both unique and shared arsenals of secreted proteins (SPs) during infection, and addresses the question of whether alterations to shared SPs or species-specific SPs play a more important role in host-specificity. To answer these questions, we annotated and compared the secretomes of three species from the Microbotryum genus of anther smuts, two closely related sister species that are able to infect each other’s hosts, albeit to reduced degrees, M. lychnidis-dioicae and M. silenes-dioicae, and one distantly related species that is unable to infect either of the other two species’ host plants and vice versa, M. violaceum var. paradoxa. We then characterized the function of the core SP MVLG_02245, an SP found in all three species with differing levels of conservation at the amino acid sequence level, and tested the importance of two species-specific SPs in host specificity, MvSl_01693 and MvSd_09295, via heterologous expression in each sister species. Finally, for future research into the role of SPs in host pathogenicity, we established a site-specific knockout system in Microbotryum using CRISPR Cas9 technology. Our results demonstrate that while host specificity in the Microbotryum genus is likely the result of alterations to the amino acid sequence of several core SPs, expression of novel SPs can have dramatic effects on pathogenicity. The research is therefore the first to identify key proteins involved in host specificity of the Microbotryum genus, and the first to establish a means of site-specific gene modification and knockout in the Microbotryum system using a CRISPR Cas9