Automatic behavior recognition in laboratory animals using kinect

Tese de Mestrado Integrado. Bioengenharia. Faculdade de Engenharia. Universidade do Porto. 201

Effect of lethal yellow (AY) mutation and photoperiod alterations on mouse behavior

Decrease in natural illumination in fall/winter months causes depressive-like seasonal affective disorders in vulnerable individuals. Obesity is another risk factor of depression. The lethal yellow (AY) mutation causes ectopic expression of agouti protein in the brain. Mice heterozygous for AY mutation (AY/a) are obese compared to their wild-type littermates (a/a). The main aims of the study were to investigate the effects of AY mutation, photoperiod and the interaction between these factors on daily activity dynamics, feeding, locomotor and exploratory activities, anxiety-related and depressive-like behaviors in mild stress condition. Six weeks old mouse males of AY/a and a/a lines were divided into four groups eight animals each and exposed to long- (14 h light and 10 h darkness) or short- (4 h light and 20 h darkness) day conditions for 28 days. Then the behavior of these mice was successively investigated in the home cage, open field, elevated plus-maze and forced swim tests. We did not observed any effect of AY mutation on the general activity, water and food consumption in the home cage; locomotion and exploration in the open field test; anxiety-related behavior in the open field and elevated plus-maze tests. At the same time, AY mutation increased depressive-like immobility time in the forced swim test (F1.28 = 20.03, p = 0.00012). Shortday conditions decreased nocturnal activity in the home cage, as well as locomotion (F1.28 = 16.33, p = 0.0004) and exploration (F1.28 = 16.24, p < 0.0004) in the open field test. Moreover, short-day exposition decreased time spent in the center of the open field (F1.28 = 6.57, p = 0.016) and in the open arms of the elevated plus-maze (F1.28 = 12.08, p = 0.0017) tests and increased immobility time in the forced swim test (F1.28 = 9.95, p = 0.0038). However, no effect of the interaction between AY mutation and photoperiod on immobility time in the forced swim test was observed. Therefore, short-day photoperiod and AY mutation increased depressive-like behavior in the forced swim test by means of different mechanisms

Information Technologies for Cognitive Decline

Information technology (IT) is used to establish a diagnosis and provide treatment for people with cognitive decline. The condition affects many before it becomes clear that more permanent changes, like dementia, could be noticed. Those who search for information are exposed to lots of information and different technologies which they need to make sense of and eventually use to help themselves. In this research literature and information available on the Internet were systematically analyzed to present methods used for diagnosis and treatment. Methods used for diagnosis are self-testing, sensors, Virtual Reality (VR), and brain imaging. Methods used for treatment are games, websites with information and media, Virtual Reality (VR), sensors, and robots. The resulting concept of knowledge was the basis of an artifact whose main goal was to present the facts to the broad public. This implied that a user-friendly artifact was developed through three iterations using the Design Science framework. A total of nine users and IT usability experts have evaluated the artifact returning the SUS score of 85,83 for users and 87,5 for IT usability experts. Nielsen´s heuristics were assessed by IT usability experts only, returning an average score of 4,28. The general response was positive regarding both the content and the attempt to present methods used in cognitive decline. It reminds to be seen how to bring this knowledge to those who are most affected by the decline.Masteroppgave i informasjonsvitenskapINFO390MASV-INF

Technology assisted screening and balance training systems for stroke patients

by Deepesh KumarPh.D

Interactive exploration of historic information via gesture recognition

Developers of interactive exhibits often struggle to �nd appropriate input devices that enable intuitive control, permitting the visitors to engage e�ectively with the content. Recently motion sensing input devices like the Microsoft Kinect or Panasonic D-Imager have become available enabling gesture based control of computer systems. These devices present an attractive input device for exhibits since the user can interact with their hands and they are not required to physically touch any part of the system. In this thesis we investigate techniques to enable the raw data coming from these types of devices to be used to control an interactive exhibit. Object recognition and tracking techniques are used to analyse the user's hand where movement and clicks are processed. To show the e�ectiveness of the techniques the gesture system is used to control an interactive system designed to inform the public about iconic buildings in the centre of Norwich, UK. We evaluate two methods of making selections in the test environment. At the time of experimentation the technologies were relatively new to the image processing environment. As a result of the research presented in this thesis, the techniques and methods used have been detailed and published [3] at the VSMM (Virtual Systems and Multimedia 2012) conference with the intention of further forwarding the area

Neural dynamic adaptation of extended amygdala to stress and anxiety

Anxiety disorders constitute changes across behavioral, physiological, and psychological dimensions that are driven by expanse neural circuits. However, the bed nucleus of the stria terminalis (BNST), a component of the extended amygdala, plays a crucial role in regulating many aspects of these changes. For instance, activation of specific cells within BNST can drive freezing behavior and while others control physiological arousal. Still, many studies using rodent models have focused on only a handful of behavioral changes. Much of our understanding of the processes related to anxiety, consequently, have been largely based on a few phenotypes, such as avoidance of open and well-lit spaces. Here, I highlight experiments we performed to broaden our understanding of anxiety-related phenotypes and the specific BNST cell types involved. In the first aim, we measure changes in pupil size in the mouse as a proxy for arousal and find that prepronociceptin (Pnoc)-expressing neurons within BNST encode and drive these physiological changes. Further, calcium imaging indicates the cellular responses of these cells to both anxiogenic stimuli and changes in arousal are varied. This underlying heterogeneity may be the means for a broad role for BNST in controlling aspects of anxiety-related processes. For the second aim, we investigate how anxiety-like states can induce changes in behavior using more quantitative, computational analysis. We administer the alpha 2-adrenergic antagonist yohimbine to induce anxiety-like states in mice and find that these states produces a wealth of changes in behavioral expression. Further using traditional behavioral analysis would have failed to appreciate these alterations. Together, these studies highlight the advantages of taking a diverse approach to understanding anxiety-related phenotypes. The information we gain will likely accelerate our understanding of anxiety disorders and ultimately drive us toward better treatment for the disease.Doctor of Philosoph

Measuring Behavior 2018 Conference Proceedings

These proceedings contain the papers presented at Measuring Behavior 2018, the 11th International Conference on Methods and Techniques in Behavioral Research. The conference was organised by Manchester Metropolitan University, in collaboration with Noldus Information Technology. The conference was held during June 5th – 8th, 2018 in Manchester, UK. Building on the format that has emerged from previous meetings, we hosted a fascinating program about a wide variety of methodological aspects of the behavioral sciences. We had scientific presentations scheduled into seven general oral sessions and fifteen symposia, which covered a topical spread from rodent to human behavior. We had fourteen demonstrations, in which academics and companies demonstrated their latest prototypes. The scientific program also contained three workshops, one tutorial and a number of scientific discussion sessions. We also had scientific tours of our facilities at Manchester Metropolitan Univeristy, and the nearby British Cycling Velodrome. We hope this proceedings caters for many of your interests and we look forward to seeing and hearing more of your contributions