Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation

Protein phosphorylation and membrane proteins play an important role in the infection of plants by phytopathogenic fungi, given their involvement in signal transduction cascades. Botrytis cinerea is a well-studied necrotrophic fungus taken as a model organism in fungal plant pathology, given its broad host range and adverse economic impact. To elucidate relevant events during infection, several proteomics analyses have been performed in B. cinerea, but they cover only 10% of the total proteins predicted in the genome database of this fungus. To increase coverage, we analysed by LC-MS/MS the first-reported overlapped proteome in phytopathogenic fungi, the “phosphomembranome” of B. cinerea, combining the two most important signal transduction subproteomes. Of the 1112 membrane-associated phosphoproteins identified, 64 and 243 were classified as exclusively identified or overexpressed under glucose and deproteinized tomato cell wall conditions, respectively. Seven proteins were found under both conditions, but these presented a specific phosphorylation pattern, so they were considered as exclusively identified or overexpressed proteins. From bioinformatics analysis, those differences in the membrane-associated phosphoproteins composition were associated with various processes, including pyruvate metabolism, unfolded protein response, oxidative stress response, autophagy and cell death. Our results suggest these proteins play a significant role in the B. cinerea pathogenic cycl

Coarse-to-Fine Adaptive People Detection for Video Sequences by Maximizing Mutual Information

Applying people detectors to unseen data is challenging since patterns distributions, such as viewpoints, motion, poses, backgrounds, occlusions and people sizes, may significantly differ from the ones of the training dataset. In this paper, we propose a coarse-to-fine framework to adapt frame by frame people detectors during runtime classification, without requiring any additional manually labeled ground truth apart from the offline training of the detection model. Such adaptation make use of multiple detectors mutual information, i.e., similarities and dissimilarities of detectors estimated and agreed by pair-wise correlating their outputs. Globally, the proposed adaptation discriminates between relevant instants in a video sequence, i.e., identifies the representative frames for an adaptation of the system. Locally, the proposed adaptation identifies the best configuration (i.e., detection threshold) of each detector under analysis, maximizing the mutual information to obtain the detection threshold of each detector. The proposed coarse-to-fine approach does not require training the detectors for each new scenario and uses standard people detector outputs, i.e., bounding boxes. The experimental results demonstrate that the proposed approach outperforms state-of-the-art detectors whose optimal threshold configurations are previously determined and fixed from offline training dataThis work has been partially supported by the Spanish government under the project TEC2014-53176-R (HAVideo

Curriculum Domain Adaptation for Semantic Segmentation of Urban Scenes

During the last half decade, convolutional neural networks (CNNs) have triumphed over semantic segmentation, which is one of the core tasks in many applications such as autonomous driving. However, to train CNNs requires a considerable amount of data, which is difficult to collect and laborious to annotate. Recent advances in computer graphics make it possible to train CNNs on photo-realistic synthetic imagery with computer-generated annotations. Despite this, the domain mismatch between the real images and the synthetic data cripples the models' performance. Hence, we propose a curriculum-style learning approach to minimize the domain gap in urban scenery semantic segmentation. The curriculum domain adaptation solves easy tasks first to infer necessary properties about the target domain; in particular, the first task is to learn global label distributions over images and local distributions over landmark superpixels. These are easy to estimate because images of urban scenes have strong idiosyncrasies (e.g., the size and spatial relations of buildings, streets, cars, etc.). We then train a segmentation network while regularizing its predictions in the target domain to follow those inferred properties. In experiments, our method outperforms the baselines on two datasets and two backbone networks. We also report extensive ablation studies about our approach.Comment: This is the extended version of the ICCV 2017 paper "Curriculum Domain Adaptation for Semantic Segmentation of Urban Scenes" with additional GTA experimen

A survey of simulation techniques in commerce and defence

Despite the developments in Modelling and Simulation (M&S) tools and techniques over the past years, there has been a gap in the M&S research and practice in healthcare on developing a toolkit to assist the modellers and simulation practitioners with selecting an appropriate set of techniques. This study is a preliminary step towards this goal. This paper presents some results from a systematic literature survey on applications of M&S in the commerce and defence domains that could inspire some improvements in the healthcare. Interim results show that in the commercial sector Discrete-Event Simulation (DES) has been the most widely used technique with System Dynamics (SD) in second place. However in the defence sector, SD has gained relatively more attention. SD has been found quite useful for qualitative and soft factors analysis. From both the surveys it becomes clear that there is a growing trend towards using hybrid M&S approaches

Online Domain Adaptation for Multi-Object Tracking

Automatically detecting, labeling, and tracking objects in videos depends first and foremost on accurate category-level object detectors. These might, however, not always be available in practice, as acquiring high-quality large scale labeled training datasets is either too costly or impractical for all possible real-world application scenarios. A scalable solution consists in re-using object detectors pre-trained on generic datasets. This work is the first to investigate the problem of on-line domain adaptation of object detectors for causal multi-object tracking (MOT). We propose to alleviate the dataset bias by adapting detectors from category to instances, and back: (i) we jointly learn all target models by adapting them from the pre-trained one, and (ii) we also adapt the pre-trained model on-line. We introduce an on-line multi-task learning algorithm to efficiently share parameters and reduce drift, while gradually improving recall. Our approach is applicable to any linear object detector, and we evaluate both cheap "mini-Fisher Vectors" and expensive "off-the-shelf" ConvNet features. We quantitatively measure the benefit of our domain adaptation strategy on the KITTI tracking benchmark and on a new dataset (PASCAL-to-KITTI) we introduce to study the domain mismatch problem in MOT.Comment: To appear at BMVC 201