Dynamic acoustic field activated cell separation (DAFACS)

Advances in diagnostics, cell and stem cell technologies drive the development of application-specific tools for cell and particle separation. Acoustic micro-particle separation offers a promising avenue for highthroughput, label-free, high recovery, cell and particle separation and isolation in regenerative medicine. Here, we demonstrate a novel approach utilizing a dynamic acoustic field that is capable of separating an arbitrary size range of cells. We first demonstrate the method for the separation of particles with different diameters between 6 and 45 μm and secondly particles of different densities in a heterogeneous medium. The dynamic acoustic field is then used to separate dorsal root ganglion cells. The shearless, label-free and low damage characteristics make this method of manipulation particularly suited for biological applications. Advantages of using a dynamic acoustic field for the separation of cells include its inherent safety and biocompatibility, the possibility to operate over large distances (centimetres), high purity (ratio of particle population, up to 100%), and high efficiency (ratio of separated particles over total number of particles to separate, up to 100%)

Point interactions in acoustics: one dimensional models

A one dimensional system made up of a compressible fluid and several mechanical oscillators, coupled to the acoustic field in the fluid, is analyzed for different settings of the oscillators array. The dynamical models are formulated in terms of singular perturbations of the decoupled dynamics of the acoustic field and the mechanical oscillators. Detailed spectral properties of the generators of the dynamics are given for each model we consider. In the case of a periodic array of mechanical oscillators it is shown that the energy spectrum presents a band structure.Comment: revised version, 30 pages, 2 figure

Modeling the effects of wind tunnel wall absorption on the acoustic radiation characteristics of propellers

Finite element theory is used to calculate the acoustic field of a propeller in a soft walled circular wind tunnel and to compare the radiation patterns to the same propeller in free space. Parametric solutions are present for a "Gutin" propeller for a variety of flow Mach numbers, admittance values at the wall, microphone position locations, and propeller to duct radius ratios. Wind tunnel boundary layer is not included in this analysis. For wall admittance nearly equal to the characteristic value of free space, the free field and ducted propeller models agree in pressure level and directionality. In addition, the need for experimentally mapping the acoustic field is discussed

High-overtone Bulk-Acoustic Resonator gravimetric sensitivity: towards wideband acoustic spectroscopy

In the context of direct detection sensors with compact dimensions, we investigate the gravimetric sensitivity of High-overtone Bulk Acoustic Resonators, through modeling of their acoustic characteristics and experiment. The high frequency characterizing such devices is expected to induce a significant effect when the acoustic field boundary conditions are modified by a thin adlayer. Furthermore, the multimode spectral characteristics is considered for wideband acoustic spectroscopy of the adlayer, once the gravimetric sensitivity dependence of the various overtones is established. Finally, means of improving the gravimetric sensitivity by confining the acoustic field in a low acoustic-impedance layer is theoretically established.Comment: 9 pages, 10 figures in J. Appl. Phys. 201

Observations of clustering inside oceanic bubble clouds and the effect on short-range acoustic propagation

It has recently been shown [Weber, T. C. et al. (Year: 2007). “Acoustic propagation through clustered bubble clouds,” IEEE J. Ocean. Eng.32, 513–523] that gas bubble clustering plays a role in determining the acoustic field characteristics of bubbly fluids. In particular, it has been shown that clustering changes the bubble-induced attenuation as well as the ping-to-ping variability in the acoustic field. The degree to which bubble clustering exists in nature, however, is unknown. This paper describes a method for quantifying bubble clustering using a high frequency (400kHz) multibeam sonar, and reports on observations of near-surface bubbleclustering during a storm ( 14.6m∕s wind speed) in the Gulf of Maine. The multibeam sonardata are analyzed to estimate the pair correlation function, a measure of bubble clustering. In order to account for clustering in the mean acoustic field, a modification to the effective mediumwave number is made. With this modification, the multibeam sonar observations are used to predict the effect of clustering on the attenuation of the mean field for short-range propagation (1 m) at frequencies between 10 and 350kHz . Results for this specific case show that clusteringcan cause the attenuation to change by 20%–80% over this frequency range

Experimental study of vibro-acoustic response of stiffened cylindrical shells

Vibrational response of stiffened cylindrical shell to reverberant acoustic field

Behavior of nozzles and acoustic liners in three-dimensional acoustic fields Quarterly report, 1 Sep. - 31 Nov. 1970

Behavior of nozzles and acoustic liners in three dimensional acoustic field

Behavior of nozzles and acoustic liners in three-dimensional acoustic fields Quarterly report, 1 Mar. - 31 May 1969

Behavior of convergent-divergent nozzles and acoustic liners in three dimensional acoustic field