Application of additive manufacturing to the digital restoration of archaeological artefacts

A review of literature showed that published applications of Additive Manufacturing (AM) to digital restoration of archaeological artefacts was rather limited. This paper reports a substantial body of work that has been done in this area. It has been used to determine how AM and subsequent processes should be optimally applied, and introduces a series of process maps that have been generated to guide future practical work. The research methodology employed was predominantly action research, where the researcher undertakes practical work in a reflective manner to develop answers to specific research questions, with a combination of questionnaires and expert interviews used for validating the process maps. The results generated from the work indicated that archaeological artefacts can be characterised according to subject, material, complexity of shape, overall size, minimum feature size, and surface finish. The optimised application of AM and subsequent processes can then be specified in response to these requirements. The outputs from the research should prove to be valuable to anyone working in the field of digital restoration and fine art sculpture, particularly when digital capture of shape and the creation of physical replicas are required. The main contribution to knowledge is the characterisation of archaeological artefacts and the resultant process maps derived from this characterisation. However, the range of projects undertaken was not representative of every combination of artefact characteristics, and some requirements could not be met fully by current AM capabilities, so there remains a need for further research on process development

Scalable Interactive Volume Rendering Using Off-the-shelf Components

This paper describes an application of a second generation implementation of the Sepia architecture (Sepia-2) to interactive volu-metric visualization of large rectilinear scalar fields. By employingpipelined associative blending operators in a sort-last configuration a demonstration system with 8 rendering computers sustains 24 to 28 frames per second while interactively rendering large data volumes (1024x256x256 voxels, and 512x512x512 voxels). We believe interactive performance at these frame rates and data sizes is unprecedented. We also believe these results can be extended to other types of structured and unstructured grids and a variety of GL rendering techniques including surface rendering and shadow map-ping. We show how to extend our single-stage crossbar demonstration system to multi-stage networks in order to support much larger data sizes and higher image resolutions. This requires solving a dynamic mapping problem for a class of blending operators that includes Porter-Duff compositing operators

On virtual auras:The cultural heritage object in the age of 3D digital reproduction

Making three-dimensional (3D) models for public-facing cultural heritage applications currently concentrates on creating digitised models that are as photo-realistic as possible. The virtual model should have, if possible, the same informational content as its subject, in order to act as a ‘digital surrogate’. Many cultural heritage objects have, to borrow an old term, aura: an affectual power to engender an emotional response in the viewer. The term ‘digital surrogate’ is commonly used when talking about 3D models in cultural heritage applications. Exploring the meaning of this term is highly valuable. The E-Curator project and subsequent work by Mona Hess has demonstrated the potential for 3D scanning technology for professional cultural heritage (CH) purposes and there are clear advantages to working in the digital realm. Visitors to CH institutions—members of the public with no privileged access to the exhibits—will have a very different experience to that of a CH professional

Evolution and Adaptation in Pseudomonas aeruginosa Biofilms Driven by Mismatch Repair System-Deficient Mutators

Pseudomonas aeruginosa is an important opportunistic pathogen causing chronic airway infections, especially in cystic fibrosis (CF) patients. The majority of the CF patients acquire P. aeruginosa during early childhood, and most of them develop chronic infections resulting in severe lung disease, which are rarely eradicated despite intensive antibiotic therapy. Current knowledge indicates that three major adaptive strategies, biofilm development, phenotypic diversification, and mutator phenotypes [driven by a defective mismatch repair system (MRS)], play important roles in P. aeruginosa chronic infections, but the relationship between these strategies is still poorly understood. We have used the flow-cell biofilm model system to investigate the impact of the mutS associated mutator phenotype on development, dynamics, diversification and adaptation of P. aeruginosa biofilms. Through competition experiments we demonstrate for the first time that P. aeruginosa MRS-deficient mutators had enhanced adaptability over wild-type strains when grown in structured biofilms but not as planktonic cells. This advantage was associated with enhanced micro-colony development and increased rates of phenotypic diversification, evidenced by biofilm architecture features and by a wider range and proportion of morphotypic colony variants, respectively. Additionally, morphotypic variants generated in mutator biofilms showed increased competitiveness, providing further evidence for mutator-driven adaptive evolution in the biofilm mode of growth. This work helps to understand the basis for the specific high proportion and role of mutators in chronic infections, where P. aeruginosa develops in biofilm communities

Evolution and adaptation in pseudomonas aeruginosa biofilms driven by mismatch repair system-deficient mutators

Pseudomonas aeruginosa is an important opportunistic pathogen causing chronic airway infections, especially in cystic fibrosis (CF) patients. The majority of the CF patients acquire P. aeruginosa during early childhood, and most of them develop chronic infections resulting in severe lung disease, which are rarely eradicated despite intensive antibiotic therapy. Current knowledge indicates that three major adaptive strategies, biofilm development, phenotypic diversification, and mutator phenotypes [driven by a defective mismatch repair system (MRS)], play important roles in P. aeruginosa chronic infections, but the relationship between these strategies is still poorly understood. We have used the flow-cell biofilm model system to investigate the impact of the mutS associated mutator phenotype on development, dynamics, diversification and adaptation of P. aeruginosa biofilms. Through competition experiments we demonstrate for the first time that P. aeruginosa MRS-deficient mutators had enhanced adaptability over wild-type strains when grown in structured biofilms but not as planktonic cells. This advantage was associated with enhanced micro-colony development and increased rates of phenotypic diversification, evidenced by biofilm architecture features and by a wider range and proportion of morphotypic colony variants, respectively. Additionally, morphotypic variants generated in mutator biofilms showed increased competitiveness, providing further evidence for mutator-driven adaptive evolution in the biofilm mode of growth. This work helps to understand the basis for the specific high proportion and role of mutators in chronic infections, where P. aeruginosa develops in biofilm communities.Fil: Lujan, Adela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Católica de Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J.; ArgentinaFil: Maciá, María D.. Instituto de Investigación Sanitaria Illes Balears; EspañaFil: Yang, Liang. Technical University of Denmark; DinamarcaFil: Molin, Søren. Technical University of Denmark; DinamarcaFil: Oliver, Antonio. Instituto de Investigación Sanitaria Illes Balears; EspañaFil: Smania, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentin

Three-Dimensional Analysis of a Viral RNA Replication Complex Reveals a Virus-Induced Mini-Organelle

Positive-strand RNA viruses are the largest genetic class of viruses and include many serious human pathogens. All positive-strand RNA viruses replicate their genomes in association with intracellular membrane rearrangements such as single- or double-membrane vesicles. However, the exact sites of RNA synthesis and crucial topological relationships between relevant membranes, vesicle interiors, surrounding lumens, and cytoplasm generally are poorly defined. We applied electron microscope tomography and complementary approaches to flock house virus (FHV)–infected Drosophila cells to provide the first 3-D analysis of such replication complexes. The sole FHV RNA replication factor, protein A, and FHV-specific 5-bromouridine 5'-triphosphate incorporation localized between inner and outer mitochondrial membranes inside ∼50-nm vesicles (spherules), which thus are FHV-induced compartments for viral RNA synthesis. All such FHV spherules were outer mitochondrial membrane invaginations with interiors connected to the cytoplasm by a necked channel of ∼10-nm diameter, which is sufficient for ribonucleotide import and product RNA export. Tomographic, biochemical, and other results imply that FHV spherules contain, on average, three RNA replication intermediates and an interior shell of ∼100 membrane-spanning, self-interacting protein As. The results identify spherules as the site of protein A and nascent RNA accumulation and define spherule topology, dimensions, and stoichiometry to reveal the nature and many details of the organization and function of the FHV RNA replication complex. The resulting insights appear relevant to many other positive-strand RNA viruses and support recently proposed structural and likely evolutionary parallels with retrovirus and double-stranded RNA virus virions

Instruments of Commerce and Knowledge: Probe Microscopy, 1980-2000

Longstanding debates about the role of the university in national culture and the global economy have entered a new phase in the past decade in most industrialized, and several industrializing, countries. One important focus of this debate is corporate involvement in academic scientific research. Proponents of the academic capitalism say that corporate involvement makes the university leaner, more agile, better able to respond to the needs of the day. Critics say that corporate involvement leaves society without the independent, critical voices traditionally lodged in universities. I argue that a science and technology studies perspective, using case studies of research communities, can push this debate in directions envisioned by neither proponents nor critics. I use the development and commercialization of the scanning tunneling microscope and the atomic force microscope as an example of how research communities continually redraw the line between corporate and academic institutions.

Building-integrated rooftop greenhouses: an energy and environmental assessment in the mediterranean context

A sustainable and secure food supply within a low-carbon and resilient infrastructure is encapsulated in several of The United Nations’ 17 sustainable development goals. The integration of urban agriculture in buildings can offer improved efficiencies; in recognition of this, the first south European example of a fully integrated rooftop greenhouse (iRTG) was designed and incorporated into the ICTA-ICP building by the Autonomous University of Barcelona. This design seeks to interchange heat, CO2 and rainwater between the building and its rooftop greenhouse. Average air temperatures for 2015 in the iRTG were 16.5 °C (winter) and 25.79 °C (summer), making the iRTG an ideal growing environment. Using detailed thermophysical fabric properties, 2015 site-specific weather data, exact control strategies and dynamic soil temperatures, the iRTG was modelled in EnergyPlus to assess the performance of an equivalent ‘freestanding’ greenhouse. The validated result shows that the thermal interchange between the iRTG and the ICTA-ICP building has considerable moderating effects on the iRTG’s indoor climate; since average hourly temperatures in an equivalent freestanding greenhouse would have been 4.1 °C colder in winter and 4.4 °C warmer in summer under the 2015 climatic conditions. The simulation results demonstrate that the iRTG case study recycled 43.78 MWh of thermal energy (or 341.93 kWh/m2/yr) from the main building in 2015. Assuming 100% energy conversion efficiency, compared to freestanding greenhouses heated with oil, gas or biomass systems, the iRTG delivered an equivalent carbon savings of 113.8, 82.4 or 5.5 kg CO2(eq)/m2/yr, respectively, and economic savings of 19.63, 15.88 or 17.33 €/m2/yr, respectively. Under similar climatic conditions, this symbiosis between buildings and urban agriculture makes an iRTG an efficient resource-management model and supports the promotion of a new typology or concept of buildings with a nexus or symbiosis between energy efficiency and food production.Postprint (published version