An Unsupervised Approach for Overlapping Cervical Cell Cytoplasm Segmentation

The poor contrast and the overlapping of cervical cell cytoplasm are the major issues in the accurate segmentation of cervical cell cytoplasm. This paper presents an automated unsupervised cytoplasm segmentation approach which can effectively find the cytoplasm boundaries in overlapping cells. The proposed approach first segments the cell clumps from the cervical smear image and detects the nuclei in each cell clump. A modified Otsu method with prior class probability is proposed for accurate segmentation of nuclei from the cell clumps. Using distance regularized level set evolution, the contour around each nucleus is evolved until it reaches the cytoplasm boundaries. Promising results were obtained by experimenting on ISBI 2015 challenge dataset.Comment: 4 pages, 4 figures, Biomedical Engineering and Sciences (IECBES), 2016 IEEE EMBS Conference on. IEEE, 201

Accurate detection of dysmorphic nuclei using dynamic programming and supervised classification

A vast array of pathologies is typified by the presence of nuclei with an abnormal morphology. Dysmorphic nuclear phenotypes feature dramatic size changes or foldings, but also entail much subtler deviations such as nuclear protrusions called blebs. Due to their unpredictable size, shape and intensity, dysmorphic nuclei are often not accurately detected in standard image analysis routines. To enable accurate detection of dysmorphic nuclei in confocal and widefield fluorescence microscopy images, we have developed an automated segmentation algorithm, called Blebbed Nuclei Detector (BleND), which relies on two-pass thresholding for initial nuclear contour detection, and an optimal path finding algorithm, based on dynamic programming, for refining these contours. Using a robust error metric, we show that our method matches manual segmentation in terms of precision and outperforms state-of-the-art nuclear segmentation methods. Its high performance allowed for building and integrating a robust classifier that recognizes dysmorphic nuclei with an accuracy above 95%. The combined segmentation-classification routine is bound to facilitate nucleus-based diagnostics and enable real-time recognition of dysmorphic nuclei in intelligent microscopy workflows

Motion Segmentation Aided Super Resolution Image Reconstruction

This dissertation addresses Super Resolution (SR) Image Reconstruction focusing on motion segmentation. The main thrust is Information Complexity guided Gaussian Mixture Models (GMMs) for Statistical Background Modeling. In the process of developing our framework we also focus on two other topics; motion trajectories estimation toward global and local scene change detections and image reconstruction to have high resolution (HR) representations of the moving regions. Such a framework is used for dynamic scene understanding and recognition of individuals and threats with the help of the image sequences recorded with either stationary or non-stationary camera systems. We introduce a new technique called Information Complexity guided Statistical Background Modeling. Thus, we successfully employ GMMs, which are optimal with respect to information complexity criteria. Moving objects are segmented out through background subtraction which utilizes the computed background model. This technique produces superior results to competing background modeling strategies. The state-of-the-art SR Image Reconstruction studies combine the information from a set of unremarkably different low resolution (LR) images of static scene to construct an HR representation. The crucial challenge not handled in these studies is accumulating the corresponding information from highly displaced moving objects. In this aspect, a framework of SR Image Reconstruction of the moving objects with such high level of displacements is developed. Our assumption is that LR images are different from each other due to local motion of the objects and the global motion of the scene imposed by non-stationary imaging system. Contrary to traditional SR approaches, we employed several steps. These steps are; the suppression of the global motion, motion segmentation accompanied by background subtraction to extract moving objects, suppression of the local motion of the segmented out regions, and super-resolving accumulated information coming from moving objects rather than the whole scene. This results in a reliable offline SR Image Reconstruction tool which handles several types of dynamic scene changes, compensates the impacts of camera systems, and provides data redundancy through removing the background. The framework proved to be superior to the state-of-the-art algorithms which put no significant effort toward dynamic scene representation of non-stationary camera systems

Computer Vision and Medical Image Processing: a brief survey of application areas

Every day is greater the number of images obtained to characterize the anatomy and functions of the human body, because of this the automation of the medical image processing has become a practice to improve the diagnosis and treatment of certain diseases. In this study the main areas of application of computer vision to the digital processing of medical images are reviewed. It begins with the selection of the three edges with more publications available in Springer, ScienceDirect, Wiley, and IEEE which are: segmentation of organs and lesions, feature extraction in optical images and labelling machine on x-ray images. Over them, latest algorithms, techniques and methods for medical imaging processing are analyzed exposing its main characteristics and ways of use.Sociedad Argentina de Informática e Investigación Operativa (SADIO

Computer Vision and Medical Image Processing: a brief survey of application areas

Every day is greater the number of images obtained to characterize the anatomy and functions of the human body, because of this the automation of the medical image processing has become a practice to improve the diagnosis and treatment of certain diseases. In this study the main areas of application of computer vision to the digital processing of medical images are reviewed. It begins with the selection of the three edges with more publications available in Springer, ScienceDirect, Wiley, and IEEE which are: segmentation of organs and lesions, feature extraction in optical images and labelling machine on x-ray images. Over them, latest algorithms, techniques and methods for medical imaging processing are analyzed exposing its main characteristics and ways of use.Sociedad Argentina de Informática e Investigación Operativa (SADIO

Generalized Kernel-based Visual Tracking

In this work we generalize the plain MS trackers and attempt to overcome standard mean shift trackers' two limitations. It is well known that modeling and maintaining a representation of a target object is an important component of a successful visual tracker. However, little work has been done on building a robust template model for kernel-based MS tracking. In contrast to building a template from a single frame, we train a robust object representation model from a large amount of data. Tracking is viewed as a binary classification problem, and a discriminative classification rule is learned to distinguish between the object and background. We adopt a support vector machine (SVM) for training. The tracker is then implemented by maximizing the classification score. An iterative optimization scheme very similar to MS is derived for this purpose.Comment: 12 page

Learning an Interactive Segmentation System

Many successful applications of computer vision to image or video manipulation are interactive by nature. However, parameters of such systems are often trained neglecting the user. Traditionally, interactive systems have been treated in the same manner as their fully automatic counterparts. Their performance is evaluated by computing the accuracy of their solutions under some fixed set of user interactions. This paper proposes a new evaluation and learning method which brings the user in the loop. It is based on the use of an active robot user - a simulated model of a human user. We show how this approach can be used to evaluate and learn parameters of state-of-the-art interactive segmentation systems. We also show how simulated user models can be integrated into the popular max-margin method for parameter learning and propose an algorithm to solve the resulting optimisation problem.Comment: 11 pages, 7 figures, 4 table