Cultures of Sustainability: ‘Ways of doing’ cooking

In our research, we have been expanding our conceptual and methodological frames of reference as designers, in order to explore the complexity of factors involved in environmental sustainability and the consequent challenges posed for design research. In this paper, we discuss some of these issues in user-centered and sustainable design, drawing out and developing relations to concepts from other fields of study, such as the sociology of consumption and material culture. In order to better understand the role that (sustainable) design products might play within people’s everyday lives and lifestyles, we interpret and discuss notions of ‘socio-cultural practices’ of consumption and frame an approach to studying people’s ‘ways of doing’ with artifacts. We point to two examples from our previous research on designing for energy awareness and for sustainable bathing practices. A current study is presented in depth, in which families and singles, resident in The Netherlands but originating from different countries, have been observed and interviewed during preparation of a meal, eating and clearing up afterwards. Through studying and reflecting on the different ‘ways of doing’ cooking, we gained insights into how cooking and a range of associated practices and artifacts are deeply embedded in traditions, meanings and aspirations. Issues of environmental consumption, such as water, energy and waste, are at stake in such design research but, as we argue, so is attention and sensitivity to how these are interwoven in meaningful socio-cultural practices. The setup and findings are presented, as a point of departure for raising conceptual and methodological questions to be developed in future work

Logical operators for ontological modeling

We show that logic has more to offer to ontologists than standard first order and modal operators. We first describe some operators of linear logic which we believe are particularly suitable for ontological modeling, and suggest how to interpret them within an ontological framework. After showing how they can coexist with those of classical logic, we analyze three notions of artifact from the literature to conclude that these linear operators allow for reducing the ontological commitment needed for their formalization, and even simplify their logical formulation

RNA-Seq optimization with eQTL gold standards.

BackgroundRNA-Sequencing (RNA-Seq) experiments have been optimized for library preparation, mapping, and gene expression estimation. These methods, however, have revealed weaknesses in the next stages of analysis of differential expression, with results sensitive to systematic sample stratification or, in more extreme cases, to outliers. Further, a method to assess normalization and adjustment measures imposed on the data is lacking.ResultsTo address these issues, we utilize previously published eQTLs as a novel gold standard at the center of a framework that integrates DNA genotypes and RNA-Seq data to optimize analysis and aid in the understanding of genetic variation and gene expression. After detecting sample contamination and sequencing outliers in RNA-Seq data, a set of previously published brain eQTLs was used to determine if sample outlier removal was appropriate. Improved replication of known eQTLs supported removal of these samples in downstream analyses. eQTL replication was further employed to assess normalization methods, covariate inclusion, and gene annotation. This method was validated in an independent RNA-Seq blood data set from the GTEx project and a tissue-appropriate set of eQTLs. eQTL replication in both data sets highlights the necessity of accounting for unknown covariates in RNA-Seq data analysis.ConclusionAs each RNA-Seq experiment is unique with its own experiment-specific limitations, we offer an easily-implementable method that uses the replication of known eQTLs to guide each step in one's data analysis pipeline. In the two data sets presented herein, we highlight not only the necessity of careful outlier detection but also the need to account for unknown covariates in RNA-Seq experiments

The Red Sea, Coastal Landscapes, and Hominin Dispersals

This chapter provides a critical assessment of environment, landscape and resources in the Red Sea region over the past five million years in relation to archaeological evidence of hominin settlement, and of current hypotheses about the role of the region as a pathway or obstacle to population dispersals between Africa and Asia and the possible significance of coastal colonization. The discussion assesses the impact of factors such as topography and the distribution of resources on land and on the seacoast, taking account of geographical variation and changes in geology, sea levels and palaeoclimate. The merits of northern and southern routes of movement at either end of the Red Sea are compared. All the evidence indicates that there has been no land connection at the southern end since the beginning of the Pliocene period, but that short sea crossings would have been possible at lowest sea-level stands with little or no technical aids. More important than the possibilities of crossing the southern channel is the nature of the resources available in the adjacent coastal zones. There were many climatic episodes wetter than today, and during these periods water draining from the Arabian escarpment provided productive conditions for large mammals and human populations in coastal regions and eastwards into the desert. During drier episodes the coastal region would have provided important refugia both in upland areas and on the emerged shelves exposed by lowered sea level, especially in the southern sector and on both sides of the Red Sea. Marine resources may have offered an added advantage in coastal areas, but evidence for their exploitation is very limited, and their role has been over-exaggerated in hypotheses of coastal colonization